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Searching for up to 100 curated homologs for 16871 FitnessBrowser__Keio:16871 (446 a.a.)

Found high-coverage hits (≥70%) to 26 curated proteins.

Removed hits that are identical to the query, leaving 22

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

1jctA Glucarate dehydratase, n341l mutant orthorhombic form
    100% identity, 99% coverage of query (920 bits)

gudD / Q6FFQ2 D-glucarate dehydratase subunit (EC 4.2.1.40) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
GUDD_ACIAD / Q6FFQ2 Glucarate dehydratase; GDH; GlucD; EC 4.2.1.40 from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
Q6FFQ2 glucarate dehydratase (EC 4.2.1.40) from Acinetobacter baylyi (see paper)
    70% identity, 99% coverage of query (689 bits)

gudD / P42206 D-glucarate dehydratase subunit (EC 4.2.1.40) from Pseudomonas putida (see paper)
GUDD_PSEPU / P42206 Glucarate dehydratase; GDH; GlucD; EC 4.2.1.40 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
    72% identity, 98% coverage of query (663 bits)

3p0wB / B2UIZ1 Crystal structure of d-glucarate dehydratase from ralstonia solanacearum complexed with mg and d-glucarate
    69% identity, 98% coverage of query (590 bits)

GudX / b2788 glucarate dehydratase-related protein from Escherichia coli K-12 substr. MG1655 (see 2 papers)
GUDX_ECOLI / Q46915 Glucarate dehydratase-related protein; GDH-RP; GlucDRP; EC 4.2.1.- from Escherichia coli (strain K12) (see paper)
gudX / RF|NP_417268 glucarate dehydratase-related protein from Escherichia coli K12
    63% identity, 100% coverage of query (570 bits)

3nxlC / Q39KL8 Crystal structure of glucarate dehydratase from burkholderia cepacia complexed with magnesium
    65% identity, 98% coverage of query (553 bits)

3nfuA / Q1QUN0 Crystal structure of probable glucarate dehydratase from chromohalobacter salexigens dsm 3043 complexed with magnesium
    59% identity, 98% coverage of query (546 bits)

3n6hB / A6VQF6 Crystal structure of mandelate racemase/muconate lactonizing protein from actinobacillus succinogenes 130z complexed with magnesium/sulfate
    60% identity, 99% coverage of query (543 bits)

3pfrA Crystal structure of d-glucarate dehydratase related protein from actinobacillus succinogenes complexed with d-glucarate
    60% identity, 99% coverage of query (541 bits)

4it1D / Q3KB33 Crystal structure of enolase pfl01_3283 (target efi-502286) from pseudomonas fluorescens pf0-1 with bound magnesium, potassium and tartrate
    35% identity, 97% coverage of query (227 bits)

3va8A Crystal structure of enolase fg03645.1 (target efi-502278) from gibberella zeae ph-1 complexed with magnesium, formate and sulfate
    34% identity, 97% coverage of query (223 bits)

3vc6A / D6Y7Y6 Crystal structure of enolase tbis_1083(target efi-502310) from thermobispora bispora dsm 43833 complexed with magnesium and formate
    34% identity, 97% coverage of query (223 bits)

Build an alignment

Build an alignment for 16871 and 12 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

D4GYD4 gluconate dehydratase (EC 4.2.1.39) from Haloferax volcanii (see paper)
    23% identity, 83% coverage of query (70.5 bits)

gci / A9CEQ8 D-galactarolactone cycloisomerase monomer (EC 5.5.1.27) from Agrobacterium fabrum (strain C58 / ATCC 33970) (see paper)
GCI_AGRFC / A9CEQ8 D-galactarolactone cycloisomerase; EC 5.5.1.27 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see 2 papers)
A9CEQ8 D-galactarolactone cycloisomerase (EC 5.5.1.27) from Agrobacterium tumefaciens (see paper)
4hpnA / A9CEQ8 Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
    25% identity, 83% coverage of query (66.2 bits)

catB / BAE46388.1 cis,cis-muconate cycloisomerase from Rhodococcus sp. AN-22 (see paper)
    24% identity, 83% coverage of query (63.2 bits)

2gghC / Q9RYA6 The mutant a68c-d72c-nlq of deinococcus radiodurans nacylamino acid racemase (see paper)
    24% identity, 74% coverage of query (55.8 bits)

xacD / D4GP40 D-xylonate dehydratase subunit (EC 4.2.1.25; EC 4.2.1.82) from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (see 3 papers)
XAD_HALVD / D4GP40 D-xylonate dehydratase; XAD; EC 4.2.1.82 from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see 2 papers)
D4GP40 xylonate dehydratase (EC 4.2.1.82) from Haloferax volcanii (see paper)
    23% identity, 83% coverage of query (55.1 bits)

3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
    23% identity, 82% coverage of query (52.4 bits)

3ozmA / A0A0H3LT39 Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
    24% identity, 86% coverage of query (50.8 bits)

3ozmD Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
    24% identity, 83% coverage of query (50.4 bits)

3op2A Crystal structure of putative mandelate racemase from bordetella bronchiseptica rb50 complexed with 2-oxoglutarate/phosphate
    25% identity, 83% coverage of query (47.8 bits)

3i6eA / Q5LM96 Crystal structure of muconate lactonizing enzyme from ruegeria pomeroyi.
    21% identity, 76% coverage of query (46.6 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory