Sites on a Tree

 

Searching for up to 100 curated homologs for 17517 b3456 leucine/isoleucine/valine transporter subunit (NCBI) (425 a.a.)

Found high-coverage hits (≥70%) to 12 curated proteins.

Removed hits that are identical to the query, leaving 11

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Pf6N2E2_2924 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, permease component 2 LivM from Pseudomonas fluorescens FW300-N2E2
    61% identity, 97% coverage of query (466 bits)

TC 3.A.1.4.8 / P21628 High-affinity branched-chain amino acid transport system permease protein BraE, component of Branched chain amino acid uptake transporter. Transports alanine from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    62% identity, 96% coverage of query (432 bits)

AZOBR_RS08240 L-proline and D-alanine ABC transporter, permease component 2 from Azospirillum brasilense Sp245
    58% identity, 76% coverage of query (360 bits)

braE / CAB75550.1 integral membrane protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    55% identity, 71% coverage of query (310 bits)

HSERO_RS00890 L-proline ABC transporter, permease component 2 from Herbaspirillum seropedicae SmR1
    40% identity, 73% coverage of query (213 bits)

TC 3.A.1.4.3 / Q8VM84 BraE aka Bra2E, component of General L- (and D-)amino acid uptake porter (transports acidic, basic, polar, semipolar and hydrophobic amino acids). The amino and carboxyl groups do not need to be α since γ-aminobutyric acid (GABA) is a substrate. The system may function with additional binding proteins since L-alanine uptake is not dependent on BraC from Rhizobium leguminosarum (biovar viciae) (see paper)
    33% identity, 72% coverage of query (136 bits)

Build an alignment

Build an alignment for 17517 and 6 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

TC 3.A.1.4.7 / Q92TN2 ABC transporter, permease, component of The protocatechuate (3,4-dihydroxybenzoate) uptake porter, PcaMNVWX from Rhizobium meliloti (strain 1021)
    28% identity, 73% coverage of query (94.4 bits)

TC 3.A.1.4.4 / Q9L3S4 UrtC, component of The high-affinity (<1 μM) urea porter (see paper)
    24% identity, 74% coverage of query (77.4 bits)

TC 3.A.1.4.5 / Q8NRV9 UrtC, component of The high affinity urea/thiourea/hydroxyurea porter from Corynebacterium glutamicum (see paper)
    29% identity, 70% coverage of query (71.6 bits)

braD / CAB75549.1 integral membrane protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    25% identity, 70% coverage of query (48.9 bits)

LivH / b3457 branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH (EC 7.4.2.2) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
LivH / P0AEX7 branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH (EC 7.4.2.2) from Escherichia coli (strain K12) (see 4 papers)
TC 3.A.1.4.1 / P0AEX7 High-affinity branched-chain amino acid transport system permease protein LivH aka B3457, component of Leucine; leucine/isoleucine/valine porter from Escherichia coli (see 6 papers)
livH high-affinity branched-chain amino acid ABC transporter, permease protein LivH from Escherichia coli K12 (see 6 papers)
    27% identity, 70% coverage of query (46.6 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory