Searching for up to 100 curated homologs for 18321 b4297 KpLE2 phage-like element; predicted dehydratase (NCBI) (655 a.a.)

Found high-coverage hits (≥70%) to 31 curated proteins.

Removed hits that are identical to the query, leaving 30

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

YagF / b0269 CP4-6 prophage; D-xylonate dehydratase (EC 4.2.1.82) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
YAGF_ECOLI / P77596 D-xylonate dehydratase YagF; EC 4.2.1.82 from Escherichia coli (strain K12) (see 2 papers)
yagF / P77596 CP4-6 prophage; D-xylonate dehydratase (EC 4.2.1.82) from Escherichia coli (strain K12) (see 4 papers)
    68% identity, 98% coverage of query (899 bits)

6ovtA / P9WKJ5 Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
    34% identity, 78% coverage of query (224 bits)

ILVD_MYCTU / P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
    34% identity, 78% coverage of query (224 bits)

Q0K7F8 dihydroxy-acid dehydratase (EC 4.2.1.9) from Cupriavidus necator (see paper)
ilvD / Q0K7F8 dihydroxyacid dehydratase (EC 4.2.1.9) from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (see paper)
    35% identity, 72% coverage of query (205 bits)

ilvDD / O27498 dihydroxy-acid dehydratase subunit (EC 4.2.1.9) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (see 4 papers)
    32% identity, 77% coverage of query (195 bits)

ILVD_ARATH / Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q9LIR4 dihydroxy-acid dehydratase (EC 4.2.1.9) from Arabidopsis thaliana (see 2 papers)
    32% identity, 71% coverage of query (194 bits)

5ze4A / Q9LIR4 The structure of holo- structure of dhad complex with [2fe-2s] cluster (see paper)
    32% identity, 71% coverage of query (194 bits)

SM_b20890 L-arabonate dehydratase (EC 4.2.1.25) from Sinorhizobium meliloti 1021
    32% identity, 71% coverage of query (192 bits)

HSERO_RS05205 L-arabonate dehydratase (EC 4.2.1.25) from Herbaspirillum seropedicae SmR1
    34% identity, 71% coverage of query (189 bits)

BPHYT_RS19730 L-arabonate dehydratase (EC 4.2.1.25) from Burkholderia phytofirmans PsJN
    33% identity, 71% coverage of query (189 bits)

IlvD / b3771 dihydroxy-acid dehydratase (EC 4.2.1.9) from Escherichia coli K-12 substr. MG1655 (see paper)
ILVD_ECOLI / P05791 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Escherichia coli (strain K12) (see 4 papers)
ilvD / P05791 dihydroxy-acid dehydratase (EC 4.2.1.9) from Escherichia coli (strain K12) (see 14 papers)
ilvD / EW|b3771 dihydroxyacid dehydratase from Escherichia coli K12 (see 2 papers)
    30% identity, 80% coverage of query (188 bits)

ARAD_RHILW / B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
I9XDU6 L-Arabinonate dehydratase (EC 4.2.1.25) from Rhizobium leguminosarum bv. trifolii (see 2 papers)
    31% identity, 81% coverage of query (187 bits)

5j85A / I9XDU6 Ser480ala mutant of l-arabinonate dehydratase (see paper)
    30% identity, 81% coverage of query (185 bits)

EDD_ZYMMO / P21909 Phosphogluconate dehydratase; 6-phosphogluconate dehydratase; EC 4.2.1.12 from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) (see paper)
    31% identity, 76% coverage of query (182 bits)

ILVD_CAUVC / P55186 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
    31% identity, 75% coverage of query (182 bits)

ILVD_RHIL3 / Q1MIB2 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Rhizobium leguminosarum bv. viciae (strain 3841) (see paper)
    30% identity, 79% coverage of query (182 bits)

8epzA / A0A4R3LQ44 Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
    31% identity, 82% coverage of query (181 bits)

8ej0A Dihydroxyacid dehydratase
    31% identity, 82% coverage of query (181 bits)

Pf6N2E2_609 L-arabonate dehydratase (EC 4.2.1.25) from Pseudomonas fluorescens FW300-N2E2
    32% identity, 70% coverage of query (177 bits)

AO356_24585 L-arabonate dehydratase (EC 4.2.1.25) from Pseudomonas fluorescens FW300-N2C3
    32% identity, 73% coverage of query (174 bits)

Q8NQZ9 dihydroxy-acid dehydratase (EC 4.2.1.9) from Corynebacterium glutamicum (see 2 papers)
    30% identity, 79% coverage of query (172 bits)

Shewana3_2070 L-arabonate dehydratase (EC 4.2.1.25) from Shewanella sp. ANA-3
    31% identity, 72% coverage of query (164 bits)

ILVD1_RHIME / Q92RP0 Putative dehydratase IlvD1; EC 4.2.1.- from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
    31% identity, 71% coverage of query (158 bits)

ILVC_ASPFU / Q4X099 Dihydroxy-acid dehydratase ilvC, mitochondrial; DHAD ilvC; EC 4.2.1.9 from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    31% identity, 71% coverage of query (152 bits)

salH / B0L7G4 dihydroxy-acid dehydratase from Salinispora tropica (see paper)
    30% identity, 72% coverage of query (150 bits)

edd / CAE53634.1 6-phosphogluconate dehydratase from Nonomuraea gerenzanensis (see paper)
    31% identity, 74% coverage of query (146 bits)

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Build an alignment for 18321 and 26 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

SGD_PSEPU / P0DOV7 6-deoxy-6-sulfo-D-gluconate dehydratase; SG dehydratase; EC 4.2.1.162 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
    28% identity, 78% coverage of query (142 bits)

7m3kA / Q2YJ21 Crystal structure of galactonate dehydratase from brucella melitensis biovar abortus 2308
    29% identity, 77% coverage of query (132 bits)

Pf6N2E2_1668 Xylonate dehydratase (EC 4.2.1.82) from Pseudomonas fluorescens FW300-N2E2
    27% identity, 75% coverage of query (128 bits)

AO356_28760 Xylonate dehydratase (EC 4.2.1.82) from Pseudomonas fluorescens FW300-N2C3
    27% identity, 75% coverage of query (128 bits)

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