Sites on a Tree

 

Searching for up to 100 curated homologs for 208241 MicrobesOnline__882:208241 (432 a.a.)

Found high-coverage hits (≥70%) to 40 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

4r1mA / Q8AAN6 Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
    60% identity, 98% coverage of query (544 bits)

4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
    59% identity, 98% coverage of query (535 bits)

paaF / CAD76926.1 phenylacetate-CoA ligase from Pseudomonas sp. Y2 (see paper)
    48% identity, 99% coverage of query (402 bits)

PAAK_THET2 / Q72K16 Phenylacetate-coenzyme A ligase; Phenylacetyl-CoA ligase; PA-CoA ligase; EC 6.2.1.30 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
Q72K16 phenylacetate-CoA ligase (EC 6.2.1.30) from Thermus thermophilus (see paper)
    49% identity, 98% coverage of query (392 bits)

PAAK_PSEPU / O33469 Phenylacetate-coenzyme A ligase; Phenylacetyl-CoA ligase; PA-CoA ligase; EC 6.2.1.30 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
    47% identity, 99% coverage of query (390 bits)

B4EL89 phenylacetate-CoA ligase (EC 6.2.1.30) from Burkholderia cenocepacia (see 2 papers)
    47% identity, 98% coverage of query (385 bits)

2y4oA / B4EL89 Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
    47% identity, 98% coverage of query (385 bits)

2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate
    47% identity, 98% coverage of query (384 bits)

B4E7B5 phenylacetate-CoA ligase (EC 6.2.1.30) from Burkholderia cenocepacia (see 2 papers)
    46% identity, 98% coverage of query (378 bits)

2y27B / B4E7B5 Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
    46% identity, 98% coverage of query (378 bits)

2y4nA Paak1 in complex with phenylacetyl adenylate
    46% identity, 98% coverage of query (370 bits)

PaaK / b1398 phenylacetate-CoA ligase (EC 6.2.1.30) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
PAAK_ECOLI / P76085 Phenylacetate-coenzyme A ligase; Phenylacetyl-CoA ligase; PA-CoA ligase; EC 6.2.1.30 from Escherichia coli (strain K12) (see 3 papers)
    45% identity, 99% coverage of query (364 bits)

paaK / Q9L9C1 phenylacetate-CoA ligase (aerobic) (EC 6.2.1.30) from Aromatoleum evansii (see paper)
PAAK_AROEV / Q9L9C1 Phenylacetate-coenzyme A ligase; Phenylacetyl-CoA ligase; PA-CoA ligase; EC 6.2.1.30 from Aromatoleum evansii (Azoarcus evansii) (see 3 papers)
pacD / CAC10605.1 aerobic phenylacetate-CoA ligase from Aromatoleum evansii (see paper)
    44% identity, 98% coverage of query (359 bits)

mdpP / A2SP20 2-hydroxyisobutanoate-CoA ligase from Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1) (see paper)
    33% identity, 99% coverage of query (208 bits)

6he0A / I3VE75 Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
    33% identity, 99% coverage of query (208 bits)

6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp
    32% identity, 99% coverage of query (197 bits)

6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp
    32% identity, 99% coverage of query (197 bits)

bal / Q5P5G7 benzoylacetate-CoA ligase from Aromatoleum aromaticum (strain EbN1) (see paper)
    35% identity, 86% coverage of query (183 bits)

ftsA / Q50751 factor 390 synthetase from Methanothermobacter thermautotrophicus (see 3 papers)
    33% identity, 72% coverage of query (152 bits)

6sixB / A0A022MRT4 Paak family amp-ligase with anp (see paper)
    33% identity, 78% coverage of query (138 bits)

6siyA Paak family amp-ligase with amp and substrate
    33% identity, 78% coverage of query (138 bits)

6siwA Paak family amp-ligase with amp
    33% identity, 78% coverage of query (138 bits)

Build an alignment

Build an alignment for 208241 and 22 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

ptmA1 / D8L2T7 7α-hydroxy-ent-kauranol-carboxylate adenylase from Streptomyces platensis (see paper)
    28% identity, 98% coverage of query (122 bits)

ind3 / A0A0D4BST1 4,5-dehydro-L-arginine--indolmycenate ligase from Streptomyces griseus subsp. griseus (see paper)
    29% identity, 74% coverage of query (104 bits)

bcl / BAE91925.1 benzoate-CoA ligase from Magnetospirillum sp. TS-6 (see paper)
    26% identity, 78% coverage of query (62.4 bits)

RR42_RS13535 2-aminobenzoate-CoA ligase (EC 6.2.1.32) from Cupriavidus basilensis FW507-4G11
    25% identity, 82% coverage of query (58.5 bits)

PKSJ_BACSU / P40806 Polyketide synthase PksJ; PKS from Bacillus subtilis (strain 168) (see 3 papers)
    27% identity, 78% coverage of query (58.2 bits)

Q3LB11 benzoate-CoA ligase (EC 6.2.1.25) from Rhodopseudomonas palustris (see paper)
    26% identity, 79% coverage of query (57.0 bits)

6e8oA Crystal structure of aryl acid adenylating enzyme fscc from fuscachelin nrps in complex with amp
    26% identity, 79% coverage of query (57.0 bits)

hbaA / Q53005 4-hydroxybenzoate-CoA ligase subunit (EC 6.2.1.25; EC 6.2.1.27) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) (see paper)
4HBCL_RHOPA / Q53005 4-hydroxybenzoate--CoA/benzoate--CoA ligase; 4-hydroxybenzoyl-CoA synthetase; EC 6.2.1.25; EC 6.2.1.27 from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) (see paper)
    25% identity, 81% coverage of query (56.6 bits)

6e97B / Q47NR5 Crystal structure of the aryl acid adenylating enzyme fscc from fuscachelin nrps in complex with dhb-adenylate
    26% identity, 79% coverage of query (56.6 bits)

LCFB_BACSU / O07610 Long-chain-fatty-acid--CoA ligase; Long-chain acyl-CoA synthetase; EC 6.2.1.3 from Bacillus subtilis (strain 168) (see paper)
    25% identity, 76% coverage of query (56.6 bits)

6m2uA The crystal structure of benzoate coenzyme a ligase double mutant (h333a/i334a) in complex with 2-chloro-1,3-thiazole-5-carboxylate-amp
    28% identity, 79% coverage of query (56.2 bits)

6m2tA The crystal structure of benzoate coenzyme a ligase double mutant (h333a/i334a) in complex with 2-methyl-thiazole-5 carboxylate-amp
    28% identity, 79% coverage of query (56.2 bits)

badA / Q59760 benzoate-CoA ligase (EC 6.2.1.25) from Rhodopseudomonas palustris (see 2 papers)
    26% identity, 79% coverage of query (54.7 bits)

4rlfB / Q93TK0 Crystal structure of a benzoate coenzyme a ligase with p-toluic acid and o-toluic acid (see paper)
    26% identity, 79% coverage of query (54.7 bits)

4rm2A Crystal structure of a benzoate coenzyme a ligase with 2-fluoro benzoic acid
    26% identity, 79% coverage of query (54.7 bits)

4zjzA Crystal structure of a benzoate coenzyme a ligase with benzoyl-amp
    26% identity, 79% coverage of query (54.7 bits)

4rm3A Crystal structure of a benzoate coenzyme a ligase with 2-furoic acid
    26% identity, 79% coverage of query (54.7 bits)

4rmnA Crystal structure of a benzoate coenzyme a ligase with 2-thiophene carboxylic acid
    26% identity, 79% coverage of query (54.3 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory