Sites on a Tree

 

Searching for up to 100 curated homologs for 3610098 FitnessBrowser__Dino:3610098 (389 a.a.)

Found high-coverage hits (≥70%) to 42 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

pobA / AAA73519.1 4-hydroxybenzoate hydroxylase from Rhizobium leguminosarum (see paper)
    61% identity, 100% coverage of query (492 bits)

PHHY_PSEAE / P20586 p-hydroxybenzoate hydroxylase; PHBH; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 7 papers)
1d7lA / P20586 Structure-function correlations of the reaction of reduced nicotinamide analogs with p-hydroxybenzoate hydroxylase substituted with a series of 8-substituted flavins (see paper)
    58% identity, 100% coverage of query (472 bits)

1iusA P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate at ph 5.0
    58% identity, 100% coverage of query (472 bits)

1dodA The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis
    58% identity, 100% coverage of query (472 bits)

pobA / P00438 p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens (see 2 papers)
PHHY_PSEFL / P00438 p-hydroxybenzoate hydroxylase; PHBH; PHBHase; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 from Pseudomonas fluorescens (see 13 papers)
    58% identity, 100% coverage of query (471 bits)

1pbcA Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring
    58% identity, 100% coverage of query (471 bits)

2phhA The coenzyme analogue adenosine 5-diphosphoribose displaces fad in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation
    58% identity, 100% coverage of query (471 bits)

1pdhA Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified fad present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin
    58% identity, 100% coverage of query (471 bits)

1bf3A P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 replaced by ser (c116s) and arg 42 replaced by lys (r42k), in complex with fad and 4-hydroxybenzoic acid
    58% identity, 100% coverage of query (470 bits)

1k0lA Pseudomonas aeruginosa phbh r220q free of p-ohb
    58% identity, 100% coverage of query (470 bits)

1k0jA Pseudomonas aeruginosa phbh r220q in complex with NADPH and free of p- ohb
    58% identity, 100% coverage of query (470 bits)

RR42_RS21940 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Cupriavidus basilensis FW507-4G11
    60% identity, 100% coverage of query (469 bits)

Pf6N2E2_2914 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Pseudomonas fluorescens FW300-N2E2
    57% identity, 100% coverage of query (463 bits)

1ykjB A45g p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bound
    58% identity, 100% coverage of query (462 bits)

pobA / Q9R9T1 p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas putida (see paper)
Q9R9T1 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Pseudomonas putida (see paper)
    57% identity, 100% coverage of query (454 bits)

6dllB / Q88H28 2.2 angstrom resolution crystal structure of p-hydroxybenzoate hydroxylase from pseudomonas putida in complex with fad. (see paper)
    56% identity, 100% coverage of query (454 bits)

pobA / F2JUE7 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1) (see paper)
    55% identity, 100% coverage of query (448 bits)

7on9A / C4TP09 Crystal structure of para-hydroxybenzoate-3-hydroxylase prai (see paper)
    50% identity, 100% coverage of query (381 bits)

PRAI_PAESP / C4TP09 4-hydroxybenzoate 3-monooxygenase (NAD(P)H); 4-hydroxybenzoate 3-hydroxylase; 4HB 3-hydroxylase; EC 1.14.13.33 from Paenibacillus sp. (see paper)
praI / BAH79107.1 4-hydroxybenzoate 3-hydroxylase from Paenibacillus sp. JJ-1b (see paper)
    50% identity, 100% coverage of query (381 bits)

8jqoA Protocatecuate hydroxylase from xylophilus ampelinus complexed with imidazole (see paper)
    44% identity, 100% coverage of query (349 bits)

8jqoD Protocatecuate hydroxylase from xylophilus ampelinus complexed with imidazole (see paper)
    37% identity, 99% coverage of query (251 bits)

Build an alignment

Build an alignment for 3610098 and 21 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

SDNN_SORAA / A0A1B4XBH7 FAD-dependent monooxygenase sdnN; Sordarin/hypoxysordarin biosynthesis cluster protein N; EC 1.-.-.- from Sordaria araneosa (Pleurage araneosa) (see paper)
    24% identity, 92% coverage of query (63.2 bits)

7yj0D Structural basis of oxepinone formation by a flavin-monooxygenase vibo
    26% identity, 79% coverage of query (63.2 bits)

vppD / A0A075XAG5 6-hydroxy-3-succinoyl-pyridine hydroxylase (EC 1.14.13.163) from Ochrobactrum sp. SJY1 (see 2 papers)
A0A075XAG5 6-hydroxy-3-succinoylpyridine 3-monooxygenase (EC 1.14.13.163) from Ochrobactrum sp. (see paper)
    26% identity, 80% coverage of query (58.2 bits)

W8E0Y0 6-hydroxy-3-succinoylpyridine 3-monooxygenase (EC 1.14.13.163) from Agrobacterium tumefaciens (see 2 papers)
    26% identity, 80% coverage of query (57.4 bits)

ROX_NOCFA / Q5YTV5 Rifampicin monooxygenase; RIFMO; EC 1.14.13.211 from Nocardia farcinica (strain IFM 10152) (see 3 papers)
Q5YTV5 rifampicin monooxygenase (EC 1.14.13.211) from Nocardia farcinica (see paper)
    26% identity, 78% coverage of query (57.4 bits)

6c7sA Structure of rifampicin monooxygenase with product bound
    26% identity, 78% coverage of query (57.4 bits)

5koxA Structure of rifampicin monooxygenase complexed with rifampicin
    26% identity, 78% coverage of query (57.4 bits)

5kowA / Q5YTV5 Structure of rifampicin monooxygenase (see paper)
    26% identity, 78% coverage of query (57.0 bits)

PYRE3_STRRG / K7QRJ5 Dialkyldecalin synthase; FAD-dependent [4+2] cyclase; EC 5.5.1.- from Streptomyces rugosporus (see 2 papers)
    25% identity, 79% coverage of query (55.8 bits)

hspB / F8G0M4 6-hydroxy-3-succinoyl-pyridine hydroxylase monomer (EC 1.14.13.163) from Pseudomonas putida (strain DSM 28022 / S16) (see paper)
HSPB_PSEP6 / F8G0M4 6-hydroxy-3-succinoylpyridine 3-monooxygenase HspB; 6-hydroxy-3-succinoylpyridine hydroxylase; HSP hydroxylase; EC 1.14.13.163 from Pseudomonas putida (strain DSM 28022 / S16) (see 2 papers)
F8G0M4 6-hydroxy-3-succinoylpyridine 3-monooxygenase (EC 1.14.13.163) from Pseudomonas putida (see paper)
    26% identity, 83% coverage of query (55.1 bits)

oxyS / L8EUQ6 anhydrotetracycline monooxygenase monomer (EC 1.14.13.234; EC 1.14.13.38) from Streptomyces rimosus subsp. rimosus (strain ATCC 10970 / DSM 40260 / JCM 4667 / NRRL 2234) (see 6 papers)
OXYS_STRR1 / L8EUQ6 12-dehydrotetracycline 5-monooxygenase/anhydrotetracycline 6-monooxygenase; EC 1.14.13.234; EC 1.14.13.38 from Streptomyces rimosus subsp. rimosus (strain ATCC 10970 / DSM 40260 / JCM 4667 / NRRL 2234) (see paper)
L8EUQ6 5a,11a-dehydrotetracycline 5-monooxygenase (EC 1.14.13.234) from Streptomyces rimosus subsp. rimosus (see paper)
    27% identity, 78% coverage of query (54.7 bits)

5xgvA / K7QRJ5 The structure of diels-alderase pyre3 in the biosynthetic pathway of pyrroindomycins (see paper)
    25% identity, 78% coverage of query (54.7 bits)

8fhjC Crystal structure of a fad monooxygenease from methylocystis sp. Strain sb2 (see paper)
    25% identity, 79% coverage of query (53.9 bits)

4k2xB / L8EUQ6 Oxys anhydrotetracycline hydroxylase from streptomyces rimosus (see paper)
    26% identity, 78% coverage of query (52.8 bits)

NODM_HYPPI / A0A2I6PIZ2 FAD-dependent monooxygenase nodM; Nodulisporic acid biosynthesis cluster protein M; EC 1.-.-.- from Hypoxylon pulicicidum (see paper)
    22% identity, 89% coverage of query (51.2 bits)

6j0zC / Q6VMI4 Crystal structure of alpk (see paper)
    27% identity, 79% coverage of query (50.4 bits)

OTCC_STRRM / Q58PK7 Anhydrotetracycline monooxygenase; EC 1.14.13.38 from Streptomyces rimosus (see paper)
Q58PK7 anhydrotetracycline 6-monooxygenase (EC 1.14.13.38) from Streptomyces rimosus (see paper)
    26% identity, 78% coverage of query (50.4 bits)

7oujAAA / Q8KY42 7oujAAA (see paper)
    26% identity, 78% coverage of query (50.1 bits)

DPMAE_METAN / P9WEY1 FAD-dependent monooxygenase dpmaE; Diterpenoid pyrone biosynthesis cluster protein E; EC 1.-.-.- from Metarhizium anisopliae (Entomophthora anisopliae) (see paper)
    22% identity, 81% coverage of query (49.3 bits)

NTNK_NECSZ / A0A455M7R7 FAD-dependent monooxygenase ntnK; Nectripenoid biosynthesis cluster protein K; EC 1.-.-.- from Nectria sp. (see paper)
    23% identity, 80% coverage of query (48.5 bits)

7vwpB / A0A136PK59 Structure of the flavin-dependent monooxygenase flso1 from the biosynthesis of fluostatinsin
    26% identity, 79% coverage of query (47.8 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory