Sites on a Tree

 

Searching for up to 100 curated homologs for 7026499 FitnessBrowser__ANA3:7026499 (264 a.a.)

Found high-coverage hits (≥70%) to 59 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

NIT1_YEREN / P0DP68 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; yeNit1; EC 3.5.1.128 from Yersinia enterocolitica (see paper)
P0DP68 deaminated glutathione amidase (EC 3.5.1.128) from Yersinia enterocolitica (see paper)
    44% identity, 97% coverage of query (240 bits)

Q9HVU6 deaminated glutathione amidase (EC 3.5.1.128) from Pseudomonas aeruginosa (see paper)
    50% identity, 90% coverage of query (227 bits)

NILP2_ARATH / Q94JV5 Deaminated glutathione amidase, chloroplastic/cytosolic; dGSH amidase; Nitrilase-like protein 2; Protein nitrilase 1 homolog; AtNit1; Protein Nit1 homolog; EC 3.5.1.128 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q94JV5 deaminated glutathione amidase (EC 3.5.1.128) from Arabidopsis thaliana (see paper)
    42% identity, 88% coverage of query (190 bits)

NIT1_SYNYG / P0DP66 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; syNit1; EC 3.5.1.128 from Synechocystis sp. (strain PCC 6803 / GT-S) (see paper)
P0DP66 deaminated glutathione amidase (EC 3.5.1.128) from Synechocystis sp. PCC 6803 (see paper)
    41% identity, 91% coverage of query (174 bits)

NFT1_DROME / O76464 Nitrilase and fragile histidine triad fusion protein NitFhit; NFT-1 protein; EC 3.6.1.29; EC 3.5.-.- from Drosophila melanogaster (Fruit fly) (see paper)
    40% identity, 91% coverage of query (169 bits)

NFT1_CAEEL / O76463 Nitrilase and fragile histidine triad fusion protein NitFhit; EC 3.6.1.29; EC 3.5.-.- from Caenorhabditis elegans (see 2 papers)
    38% identity, 88% coverage of query (164 bits)

NIT2_YEAST / P47016 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
P47016 deaminated glutathione amidase (EC 3.5.1.128) from Saccharomyces cerevisiae (see paper)
    36% identity, 95% coverage of query (163 bits)

4hg3A / P47016 Structural insights into yeast nit2: wild-type yeast nit2 in complex with alpha-ketoglutarate (see paper)
    36% identity, 95% coverage of query (162 bits)

4hg5A Structural insights into yeast nit2: wild-type yeast nit2 in complex with oxaloacetate
    36% identity, 95% coverage of query (162 bits)

NIT2_HUMAN / Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 4 papers)
Q9NQR4 omega-amidase (EC 3.5.1.3) from Homo sapiens (see paper)
    36% identity, 89% coverage of query (161 bits)

NIT2_RAT / Q497B0 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Rattus norvegicus (Rat) (see 2 papers)
    34% identity, 90% coverage of query (160 bits)

4hgdA Structural insights into yeast nit2: c169s mutant of yeast nit2 in complex with an endogenous peptide-like ligand
    36% identity, 95% coverage of query (160 bits)

NIT3_YEAST / P49954 Omega-amidase NIT3; Nitrilase homolog 2; EC 3.5.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
    34% identity, 93% coverage of query (157 bits)

Nit2 / Q9JHW2 ω-amidase NIT2 monomer (EC 3.5.1.3) from Mus musculus (see 2 papers)
NIT2_MOUSE / Q9JHW2 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Mus musculus (Mouse) (see 3 papers)
    33% identity, 93% coverage of query (157 bits)

B8C1M9 nitrilase (EC 3.5.5.1) from Thalassiosira pseudonana (see paper)
    33% identity, 93% coverage of query (141 bits)

A0A0E3D8K7 nitrilase (EC 3.5.5.1); Aliphatic nitrilase (EC 3.5.5.7) from Fusarium proliferatum (see paper)
    34% identity, 89% coverage of query (134 bits)

B8A0E5 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    35% identity, 90% coverage of query (132 bits)

NILP3_ARATH / Q8RUF8 Omega-amidase, chloroplastic; Nitrilase-like protein 3; EC 3.5.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q8RUF8 omega-amidase (EC 3.5.1.3) from Arabidopsis thaliana (see paper)
    32% identity, 83% coverage of query (131 bits)

B8A2V8 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    33% identity, 83% coverage of query (125 bits)

mtnU / O31664 2-oxoglutaramate:glutamine aminotransferase (EC 3.5.1.3) from Bacillus subtilis (strain 168) (see 4 papers)
MTNU_BACSU / O31664 2-oxoglutaramate amidase; EC 3.5.1.111 from Bacillus subtilis (strain 168) (see 2 papers)
    32% identity, 92% coverage of query (107 bits)

Build an alignment

Build an alignment for 7026499 and 20 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

YAFV_STAA4 / P0DP65 Omega-amidase YafV; saYafV; EC 3.5.1.3 from Staphylococcus aureus (strain 04-02981) (see paper)
    28% identity, 94% coverage of query (100 bits)

B3IVI7 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (see 4 papers)
    33% identity, 86% coverage of query (93.6 bits)

davA / Q88QV2 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
Q88QV2 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (see paper)
    35% identity, 80% coverage of query (93.6 bits)

YCU9_SCHPO / O59829 Probable nitrilase C965.09; EC 3.5.-.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    30% identity, 83% coverage of query (89.0 bits)

A3DHT2 nitrilase (EC 3.5.5.1) from Acetivibrio thermocellus (see paper)
    32% identity, 90% coverage of query (88.6 bits)

Pf1N1B4_2504 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N1B4
    30% identity, 85% coverage of query (88.6 bits)

AO353_07425 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2E3
    32% identity, 81% coverage of query (85.9 bits)

NRL4A_TOBAC / Q42965 Bifunctional nitrilase/nitrile hydratase NIT4A; TNIT4A; 3-cyanoalanine hydratase; Cyanoalanine nitrilase A; Nitrilase 4A; EC 3.5.5.1; EC 3.5.5.4; EC 4.2.1.65 from Nicotiana tabacum (Common tobacco) (see 3 papers)
    31% identity, 75% coverage of query (82.8 bits)

ctu / Q5NHL7 citrullinase (EC 3.5.1.20) from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
CTU_FRATT / Q5NHL7 Citrullinase; Citrulline ureidase; CTU; EC 3.5.1.20 from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
Q5NHL7 citrullinase (EC 3.5.1.20) from Francisella tularensis subsp. tularensis (see paper)
    27% identity, 91% coverage of query (82.4 bits)

NRL4B_TOBAC / Q42966 Bifunctional nitrilase/nitrile hydratase NIT4B; TNIT4B; Cyanoalanine nitrilase B; Nitrilase 4B; EC 3.5.5.1; EC 3.5.5.4 from Nicotiana tabacum (Common tobacco) (see 2 papers)
    30% identity, 75% coverage of query (81.3 bits)

D7B8P3 nitrilase (EC 3.5.5.1) from Nocardiopsis dassonvillei ATCC 23218 (see paper)
    29% identity, 78% coverage of query (81.3 bits)

ORF77 / Q93NG1 2-ketoglutaramate amidase monomer (EC 3.5.1.3) from Paenarthrobacter nicotinovorans (see 2 papers)
NIT_PAENI / Q93NG1 2-oxoglutaramate amidase; Omega-amidase; EC 3.5.1.111 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
Q93NG1 2-oxoglutaramate amidase (EC 3.5.1.111) from Paenarthrobacter nicotinovorans (see paper)
    29% identity, 88% coverage of query (81.3 bits)

NIT4A / Q5QGZ8 β-cyano-L-alanine nitrilase (EC 3.5.5.4; EC 4.2.1.65) from Lupinus angustifolius (see paper)
NRL4A_LUPAN / Q5QGZ8 Bifunctional nitrilase/nitrile hydratase NIT4A; LaNIT4A; 3-cyanoalanine hydratase; Cyanoalanine nitrilase A; EC 3.5.5.4; EC 4.2.1.65 from Lupinus angustifolius (Narrow-leaved blue lupine) (see paper)
Q5QGZ8 cyanoalanine nitrilase (EC 3.5.5.4); 3-Cyanoalanine hydratase (EC 4.2.1.65) from Lupinus angustifolius (see paper)
    31% identity, 75% coverage of query (80.1 bits)

6ypaB / O58376 The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
    29% identity, 89% coverage of query (79.7 bits)

NRL4_ARATH / P46011 Bifunctional nitrilase/nitrile hydratase NIT4; Cyanoalanine nitrilase; Nitrilase 4; EC 3.5.5.1; EC 3.5.5.4; EC 4.2.1.65 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
    30% identity, 77% coverage of query (79.7 bits)

7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide
    29% identity, 89% coverage of query (79.7 bits)

NIT4B / Q3LRV4 β-cyano-L-alanine nitrilase (EC 3.5.5.4; EC 4.2.1.65) from Lupinus angustifolius (see paper)
NRL4B_LUPAN / Q3LRV4 Bifunctional nitrilase/nitrile hydratase NIT4B; LaNIT4B; 3-cyanoalanine hydratase; Cyanoalanine nitrilase B; EC 3.5.5.4; EC 4.2.1.65 from Lupinus angustifolius (Narrow-leaved blue lupine) (see paper)
Q3LRV4 cyanoalanine nitrilase (EC 3.5.5.4) from Lupinus angustifolius (see paper)
    30% identity, 75% coverage of query (79.0 bits)

F8AEP0 amidase (EC 3.5.1.4) from Pyrococcus yayanosii (see paper)
    31% identity, 89% coverage of query (76.6 bits)

3klcA / Q9UYV8 Crystal structure of hyperthermophilic nitrilase (see paper)
    29% identity, 92% coverage of query (76.3 bits)

3klcB Crystal structure of hyperthermophilic nitrilase
    29% identity, 92% coverage of query (76.3 bits)

NITR_PYRAB / Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see 2 papers)
    29% identity, 92% coverage of query (75.1 bits)

YafV / b0219 2-oxoglutaramate amidase (EC 3.5.1.3; EC 3.5.1.111) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
yafV / Q47679 2-oxoglutaramate amidase (EC 3.5.1.3) from Escherichia coli (strain K12) (see paper)
    30% identity, 87% coverage of query (74.7 bits)

D2SGH7 nitrilase (EC 3.5.5.1) from Geodermatophilus obscurus (see paper)
    31% identity, 77% coverage of query (74.3 bits)

A3QYW3 nitrilase (EC 3.5.5.1) from Brassica rapa (see paper)
    29% identity, 77% coverage of query (74.3 bits)

R0GJB9 nitrilase (EC 3.5.5.1) from Capsella rubella (see paper)
    29% identity, 80% coverage of query (74.3 bits)

YAFV_ECOBD / A0A140NDS5 Omega-amidase YafV; ecYafV; EC 3.5.1.3 from Escherichia coli (strain B / BL21-DE3) (see paper)
    29% identity, 87% coverage of query (73.9 bits)

ramA / Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
RSAM_PSESP / Q75SP7 (R)-stereoselective amidase; EC 3.5.1.100 from Pseudomonas sp. (see paper)
Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
    32% identity, 77% coverage of query (73.6 bits)

NIT2 / P32962 Nit2 (EC 3.5.5.1; EC 4.2.1.84) from Arabidopsis thaliana (see 4 papers)
NRL2_ARATH / P32962 Nitrilase 2; EC 3.5.5.1 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
P32962 nitrilase (EC 3.5.5.1) from Arabidopsis thaliana (see paper)
    29% identity, 80% coverage of query (72.8 bits)

R0H4W3 nitrilase (EC 3.5.5.1) from Capsella rubella (see paper)
    29% identity, 80% coverage of query (70.9 bits)

NIT1 / P32961 Nit1 (EC 3.5.5.1; EC 4.2.1.84) from Arabidopsis thaliana (see 5 papers)
NRL1_ARATH / P32961 Nitrilase 1; EC 3.5.5.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
P32961 deaminated glutathione amidase (EC 3.5.1.128); nitrilase (EC 3.5.5.1) from Arabidopsis thaliana (see 6 papers)
    29% identity, 80% coverage of query (70.1 bits)

NIT3 / P46010 Nit3 (EC 3.5.5.1; EC 4.2.1.84) from Arabidopsis thaliana (see 4 papers)
P46010 nitrilase (EC 3.5.5.1) from Arabidopsis thaliana (see 2 papers)
    28% identity, 80% coverage of query (68.2 bits)

W6R265 cyanoalanine nitrilase (EC 3.5.5.4) from Pseudomonas oleovorans (see paper)
    27% identity, 77% coverage of query (68.2 bits)

Q0PIV8 nitrilase (EC 3.5.5.1) from Aeribacillus pallidus (see 2 papers)
    27% identity, 70% coverage of query (63.5 bits)

Q4KCL8 Aliphatic nitrilase (EC 3.5.5.7) from Pseudomonas fluorescens (see paper)
    28% identity, 75% coverage of query (61.2 bits)

A0A0H3LIT0 nitrilase (EC 3.5.5.1) from Bordetella bronchiseptica (see paper)
    25% identity, 86% coverage of query (56.6 bits)

K9NKH3 nitrilase (EC 3.5.5.1) from Pseudomonas sp. (see paper)
    26% identity, 75% coverage of query (54.7 bits)

D6B7V7 nitrilase (EC 3.5.5.1) from Streptomyces albus (see paper)
    26% identity, 77% coverage of query (54.3 bits)

Q1I7X1 nitrilase (EC 3.5.5.1) from Pseudomonas entomophila (see paper)
    25% identity, 76% coverage of query (54.3 bits)

A1ZD79 nitrilase (EC 3.5.5.1) from Microscilla marina (see paper)
    27% identity, 72% coverage of query (53.9 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory