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Searching for up to 100 curated homologs for AO353_04615 FitnessBrowser__pseudo3_N2E3:AO353_04615 (343 a.a.)

Found high-coverage hits (≥70%) to 10 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Pf6N2E2_5402 ABC transporter for D-Alanine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    95% identity, 100% coverage of query (674 bits)

AAPJ_RHIJ3 / Q52812 General L-amino acid-binding periplasmic protein AapJ from Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841) (Rhizobium leguminosarum bv. viciae) (see 2 papers)
TC 3.A.1.3.8 / Q52812 AapJ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) from Rhizobium leguminosarum (biovar viciae) (see 2 papers)
aapJ / CAA57933.1 general amino acid ABC type transporter from Rhizobium leguminosarum (see paper)
    56% identity, 99% coverage of query (405 bits)

SMc02118 ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, periplasmic substrate-binding component from Sinorhizobium meliloti 1021
    55% identity, 99% coverage of query (405 bits)

4z9nB / A0A0M3KL33 Abc transporter / periplasmic binding protein from brucella ovis with glutathione bound
    60% identity, 92% coverage of query (392 bits)

TC 3.A.1.3.7 / Q52663 BztA, component of Glutamate/glutamine/aspartate/asparagine porter from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (see paper)
    53% identity, 92% coverage of query (325 bits)

TC 3.A.1.3.18 / Q8YPM9 NatF, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (see paper)
    44% identity, 93% coverage of query (294 bits)

Build an alignment

Build an alignment for AO353_04615 and 6 homologs with ≥ 30% identity

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Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Additional hits (identity < 30%)

AO353_21710 ABC transporter for D-glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E3
    27% identity, 73% coverage of query (72.8 bits)

TC 2.A.56.3.1 / P74223 GtrC aka GLNH aka SLL1104, component of Tripartite glutamate:Na+ symporter (see paper)
    25% identity, 76% coverage of query (63.9 bits)

GASBP_PSEAE / Q9I402 L-glutamate/L-aspartate-binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
TC 3.A.1.3.22 / Q9I402 Probable binding protein component of ABC transporter, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    26% identity, 71% coverage of query (62.8 bits)

TC 3.A.1.3.25 / Q9CES5 Glutamine ABC transporter permease and substrate binding protein protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity from Lactococcus lactis subsp. lactis (strain IL1403)
    26% identity, 72% coverage of query (45.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory