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Searching for up to 100 curated homologs for AO353_19350 FitnessBrowser__pseudo3_N2E3:AO353_19350 (270 a.a.)

Found high-coverage hits (≥70%) to 75 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    30% identity, 100% coverage of query (111 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    30% identity, 99% coverage of query (110 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    34% identity, 92% coverage of query (103 bits)

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    35% identity, 91% coverage of query (101 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    33% identity, 96% coverage of query (88.2 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    30% identity, 95% coverage of query (87.0 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    30% identity, 95% coverage of query (87.0 bits)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    30% identity, 95% coverage of query (86.7 bits)

Build an alignment

Build an alignment for AO353_19350 and 8 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    29% identity, 98% coverage of query (85.1 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    24% identity, 92% coverage of query (79.3 bits)

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    27% identity, 91% coverage of query (78.2 bits)

hppC / AAB81313.1 2-hydroxy-6-ketonona-2,4-dienoate hydrolase from Rhodococcus globerulus (see paper)
    29% identity, 98% coverage of query (74.3 bits)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    28% identity, 99% coverage of query (73.6 bits)

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    28% identity, 99% coverage of query (71.6 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    28% identity, 96% coverage of query (71.6 bits)

BPOC_MYCTU / P9WNH1 Putative non-heme bromoperoxidase BpoC from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    30% identity, 96% coverage of query (68.9 bits)

8agsAAA / A0A1U9WZ52 8agsAAA
    26% identity, 98% coverage of query (68.9 bits)

5ng7B Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments
    26% identity, 98% coverage of query (68.9 bits)

8agpAAA Alpha/beta epoxide hydrolase
    26% identity, 98% coverage of query (68.6 bits)

8agnAAA Alpha/beta epoxide hydrolase
    26% identity, 98% coverage of query (68.6 bits)

8agmAAA Alpha/beta epoxide hydrolase
    26% identity, 98% coverage of query (68.6 bits)

A4Q9R7 haloalkane dehalogenase (EC 3.8.1.5) from Mycobacterium tuberculosis variant bovis (see paper)
    28% identity, 94% coverage of query (66.6 bits)

3ia2A Pseudomonas fluorescens esterase complexed to the r-enantiomer of a sulfonate transition state analog
    28% identity, 98% coverage of query (66.2 bits)

ESTE_PSEFL / P22862 Arylesterase; Aryl-ester hydrolase; Carboxylic acid perhydrolase; PFE; Putative bromoperoxidase; EC 3.1.1.2; EC 1.-.-.- from Pseudomonas fluorescens (see 8 papers)
GI|951089 arylesterase; EC 1.-.-.-; EC 3.1.1.2 from Pseudomonas fluorescens (see 3 papers)
AAB60168.1 esterase from Pseudomonas fluorescens (see 2 papers)
    28% identity, 98% coverage of query (65.9 bits)

G3KFX4 2-hydroxymuconate-6-semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas sp. (see paper)
    27% identity, 94% coverage of query (65.1 bits)

8pi1B / P22862 Bicyclic incypro pseudomonas fluorescens esterase (see paper)
    27% identity, 98% coverage of query (63.9 bits)

2d0dA Crystal structure of a meta-cleavage product hydrolase (cumd) a129v mutant
    26% identity, 97% coverage of query (63.5 bits)

3hi4A Switching catalysis from hydrolysis to perhydrolysis in p. Fluorescens esterase
    27% identity, 98% coverage of query (63.2 bits)

3heaA The l29p/l124i mutation of pseudomonas fluorescens esterase
    27% identity, 98% coverage of query (63.2 bits)

P96965 2-hydroxymuconate-6-semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas fluorescens (see 2 papers)
cumD / BAA12150.1 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase from Pseudomonas fluorescens (see paper)
    25% identity, 97% coverage of query (62.0 bits)

carC / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)-hexa-2,4dienoate hydrolase monomer (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
CARC_PSERE / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase; HOPDA; EC 3.7.1.13 from Pseudomonas resinovorans (see 3 papers)
Q9AQM4 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
carC / BAC41548.1 meta cleavage compound hydrolase from Pseudomonas resinovorans (see 9 papers)
    25% identity, 99% coverage of query (62.0 bits)

MhpC / b0349 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
mhpC / P77044 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli (strain K12) (see 10 papers)
MHPC_ECOLI / P77044 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase; 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; EC 3.7.1.14 from Escherichia coli (strain K12) (see 4 papers)
P77044 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli (see 4 papers)
    28% identity, 95% coverage of query (61.2 bits)

nahN / BAE92169.1 2-hydroxymuconic semialdehyde hydrolase NahN from Pseudomonas putida (see 2 papers)
    28% identity, 97% coverage of query (61.2 bits)

1iunB / P96965 Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant hexagonal (see paper)
    24% identity, 97% coverage of query (60.8 bits)

1ukaA Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with (s)-2-methylbutyrate
    24% identity, 97% coverage of query (60.8 bits)

1uk9A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with isovalerate
    24% identity, 97% coverage of query (60.8 bits)

1uk8A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-valerate
    24% identity, 97% coverage of query (60.8 bits)

1uk7A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-butyrate
    24% identity, 97% coverage of query (60.8 bits)

1iupA Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant complexed with isobutyrates
    24% identity, 97% coverage of query (60.8 bits)

Q84II3 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13) from Janthinobacterium sp. J3 (see paper)
    25% identity, 99% coverage of query (59.3 bits)

TGND_ACIAD / Q6F9F4 (E)-2-((N-methylformamido)methylene)succinate hydrolase; MFMS hydrolase; EC 3.5.1.- from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
    25% identity, 96% coverage of query (59.3 bits)

xylF / P23106 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas putida (see paper)
XYLF_PSEPU / P23106 2-hydroxymuconate semialdehyde hydrolase; HMSH; 2-hydroxymuconic semialdehyde hydrolase; EC 3.7.1.9 from Pseudomonas putida (Arthrobacter siderocapsulatus)
    28% identity, 84% coverage of query (58.9 bits)

PHYLLO / Q15KI9 PHYLLO [multifunctional] (EC 4.2.99.20; EC 3.1.2.28; EC 4.2.1.113; EC 2.2.1.9) from Arabidopsis thaliana (see 6 papers)
PHYLO_ARATH / Q15KI9 Protein PHYLLO, chloroplastic; EC 2.2.1.9; EC 4.2.1.113; EC 4.2.99.20 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    24% identity, 94% coverage of query (58.5 bits)

D0EPY0 carboxylesterase (EC 3.1.1.1) from Bacillus subtilis (see paper)
    23% identity, 93% coverage of query (58.5 bits)

NAP_BACSU / P96688 Uncharacterized carboxylesterase nap; EC 3.1.1.1 from Bacillus subtilis (strain 168)
    23% identity, 93% coverage of query (58.5 bits)

4ccwA / Q59248 Crystal structure of naproxen esterase (carboxylesterase np) from bacillus subtilis (see paper)
    23% identity, 91% coverage of query (58.2 bits)

O73957 carboxylesterase (EC 3.1.1.1) from Sulfolobus acidocaldarius (see paper)
    25% identity, 91% coverage of query (58.2 bits)

A0A0H2WW38 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (EC 4.2.99.20) from Staphylococcus aureus (see paper)
    23% identity, 93% coverage of query (57.8 bits)

YBFK_BACSU / O31452 Carboxylesterase YbfK; EC 3.1.1.1 from Bacillus subtilis (strain 168) (see paper)
    25% identity, 92% coverage of query (57.0 bits)

6i8wB / Q9KJG6 Crystal structure of a membrane phospholipase a, a novel bacterial virulence factor (see paper)
    24% identity, 97% coverage of query (57.0 bits)

Q9KJG6 triacylglycerol lipase (EC 3.1.1.3) from Pseudomonas aeruginosa (see paper)
    24% identity, 97% coverage of query (56.6 bits)

AGH13449.1 hybrid C-C meta-cleavage hydrolase-carboxylesterase from Cycloclasticus zancles (see paper)
    23% identity, 95% coverage of query (56.2 bits)

bpdF / AAB17100.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus sp. M5 (see paper)
    27% identity, 96% coverage of query (55.1 bits)

cbzF / AAX50133.1 CbzF from Pseudomonas putida (see paper)
    26% identity, 82% coverage of query (54.7 bits)

1b6gA / P22643 Haloalkane dehalogenase at ph 5.0 containing chloride (see paper)
    26% identity, 97% coverage of query (53.9 bits)

dhlA / P22643 1,2-dichloroethane dehalogenase (EC 3.8.1.5) from Xanthobacter autotrophicus (see 6 papers)
B8YPW8 haloalkane dehalogenase (EC 3.8.1.5) from Ancylobacter aquaticus (see paper)
P22643 haloalkane dehalogenase (EC 3.8.1.5) from Xanthobacter autotrophicus (see 16 papers)
    26% identity, 97% coverage of query (53.9 bits)

1cijA Haloalkane dehalogenase soaked with high concentration of bromide
    26% identity, 97% coverage of query (53.9 bits)

1be0A Haloalkane dehalogenase at ph 5.0 containing acetic acid
    26% identity, 97% coverage of query (53.9 bits)

2edaA Crystallographic and fluorescence studies of the interaction of haloalkane dehalogenase with halide ions: studies with halide compounds reveal a halide binding site in the active site
    26% identity, 97% coverage of query (53.9 bits)

2dhcA Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase
    26% identity, 97% coverage of query (53.9 bits)

P22643 Haloalkane dehalogenase; EC 3.8.1.5 from Xanthobacter autotrophicus
    26% identity, 97% coverage of query (53.9 bits)

BPHD_PARXL / P47229 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; 2,6-dioxo-6-phenylhexa-3-enoate hydrolase; EC 3.7.1.8 from Paraburkholderia xenovorans (strain LB400) (see 2 papers)
P47229 2,6-dioxo-6-phenylhexa-3-enoate hydrolase (EC 3.7.1.8) from Paraburkholderia xenovorans (see 2 papers)
BphD / CAA46911.1 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase from Pseudomonas sp (see paper)
    24% identity, 95% coverage of query (52.8 bits)

2og1A / P47229 Crystal structure of bphd, a c-c hydrolase from burkholderia xenovorans lb400 (see paper)
    24% identity, 95% coverage of query (52.8 bits)

aamsh1 / Q988D4 2-(acetamidomethylene)succinate hydrolase monomer (EC 3.5.1.29) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (see 7 papers)
AAMHY_RHILO / Q988D4 2-(acetamidomethylene)succinate hydrolase; alpha-(N-acetylaminomethylene)succinic acid amidohydrolase; AAMS amidohydrolase; EC 3.5.1.29 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
Q988D4 2-(acetamidomethylene)succinate hydrolase (EC 3.5.1.29) from Mesorhizobium loti (see 2 papers)
    25% identity, 95% coverage of query (52.0 bits)

2rhwA Crystal structure of the s112a mutant of a c-c hydrolase, bphd from burkholderia xenovorans lb400, in complex with 3,10-di-fluoro hopda
    24% identity, 95% coverage of query (51.6 bits)

2rhtA Crystal structure of the s112a mutant of a c-c hydrolase, bphd from burkholderia xenovorans lb400, in complex with 3-cl hopda
    24% identity, 95% coverage of query (51.6 bits)

2puhA Crystal structure of the s112a mutant of a c-c hydrolase, bphd from burkholderia xenovorans lb400, in complex with its substrate hopda
    24% identity, 95% coverage of query (51.6 bits)

5cbkA / A0A0M5I297 Crystal structure of the strigolactone receptor shhtl5 from striga hermonthica (see paper)
    22% identity, 93% coverage of query (50.8 bits)

8dvcA Receptor shhtl5 from striga hermonthica in complex with strigolactone agonist gr24
    22% identity, 93% coverage of query (49.7 bits)

2pujA Crystal structure of the s112a/h265a double mutant of a c-c hydrolase, bphd from burkholderia xenovorans lb400, in complex with its substrate hopda
    24% identity, 80% coverage of query (49.3 bits)

7k38A Crystal structure of pisum sativum kai2 in complex with gr24-ent5ds product (see paper)
    24% identity, 93% coverage of query (49.3 bits)

5z7xA / A0A0M4AMQ0 Crystal structure of striga hermonthica htl4 (shhtl4) (see paper)
    23% identity, 93% coverage of query (47.0 bits)

4jymA / Q9SZU7 Crystal structure of kai2 in complex with 3-methyl-2h-furo[2,3- c]pyran-2-one (see paper)
    23% identity, 93% coverage of query (46.6 bits)

KAI2_ARATH / Q9SZU7 Probable esterase KAI2; Protein DWARF-14-like; Protein D14-like; Protein HYPOSENSITIVE TO LIGHT; Protein KARRIKIN INSENSITIVE 2 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
    23% identity, 93% coverage of query (46.6 bits)

5w15D / B1YPY7 Crystal structure of an alpha/beta hydrolase fold protein from burkholderia ambifaria.
    23% identity, 96% coverage of query (43.9 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory