Sites on a Tree

 

Searching for up to 100 curated homologs for AZOBR_RS10265 FitnessBrowser__azobra:AZOBR_RS10265 (279 a.a.)

Found high-coverage hits (≥70%) to 32 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
    34% identity, 80% coverage of query (129 bits)

PHEC_PSEAE / Q01269 Cyclohexadienyl dehydratase; EC 4.2.1.51; EC 4.2.1.91 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
Q01269 arogenate dehydratase (EC 4.2.1.91) from Pseudomonas aeruginosa (see 3 papers)
    30% identity, 81% coverage of query (105 bits)

Build an alignment

Build an alignment for AZOBR_RS10265 and 2 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

3k4uE / Q7MAG0 Crystal structure of putative binding component of abc transporter from wolinella succinogenes dsm 1740 complexed with lysine
    25% identity, 80% coverage of query (97.4 bits)

5kkwA / Q4FLR5 Crystal structure of sar11_1068 bound to a sulfobetaine (3-(1- methylpiperidinium-1-yl)propane-1-sulfonate)
    25% identity, 85% coverage of query (92.4 bits)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
    28% identity, 76% coverage of query (92.0 bits)

4zv2A An ancestral arginine-binding protein bound to glutamine
    28% identity, 76% coverage of query (91.7 bits)

AO353_21710 ABC transporter for D-glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E3
    30% identity, 94% coverage of query (83.6 bits)

AO356_00480 ABC transporter for D-Glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2C3
    30% identity, 94% coverage of query (79.3 bits)

Pf6N2E2_2053 ABC transporter for D-Glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    30% identity, 84% coverage of query (75.9 bits)

6svfA / Q9WZ62 Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
    26% identity, 79% coverage of query (74.7 bits)

TcyJ / b1920 cystine ABC transporter periplasmic binding protein (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 12 papers)
tcyJ / P0AEM9 cystine ABC transporter periplasmic binding protein (EC 7.4.2.12) from Escherichia coli (strain K12) (see 13 papers)
TCYJ_ECOLI / P0AEM9 L-cystine-binding protein TcyJ; CBP; Protein FliY; Sulfate starvation-induced protein 7; SSI7 from Escherichia coli (strain K12) (see 6 papers)
TC 3.A.1.3.10 / P0AEM9 Cystine-binding periplasmic protein FLIY aka CysX aka B1920, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 7 papers)
    26% identity, 83% coverage of query (69.3 bits)

3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec)
    25% identity, 79% coverage of query (67.0 bits)

TC 3.A.1.3.17 / Q8YSA2 Basic amino acid uptake transporter, BgtAB (see paper)
    28% identity, 76% coverage of query (66.6 bits)

3vvdA / Q72JG5 Crystal structure of ttc0807 complexed with ornithine (see paper)
    25% identity, 79% coverage of query (66.2 bits)

3vvfA Crystal structure of ttc0807 complexed with arginine
    25% identity, 79% coverage of query (66.2 bits)

3vveA Crystal structure of ttc0807 complexed with lysine
    25% identity, 79% coverage of query (66.2 bits)

GlnH / b0811 L-glutamine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 13 papers)
GlnH / P0AEQ3 L-glutamine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli (strain K12) (see 13 papers)
GLNH_ECOLI / P0AEQ3 Glutamine-binding periplasmic protein; GlnBP from Escherichia coli (strain K12) (see paper)
TC 3.A.1.3.2 / P0AEQ3 Glutamine-binding periplasmic protein GlnH aka B0811 aka GlnBP, component of Three component ABC L-glutamine porter. The basal ATPase activity (ATP hydrolysis in the absence of substrate) is mainly caused by the docking of the closed-unliganded state of GlnH onto the transporter domain of GlnPQ. Unlike glutamine, arginine binds both GlnH domains, but does not trigger their closing. Comparison of the ATPase activity in nanodiscs with glutamine transport in proteoliposomes suggested that the stoichiometry of ATP per substrate is close to two from Escherichia coli (see 7 papers)
glnH / GB|AAN79369.1 glutamine ABC transporter, periplasmic glutamine-binding protein GlnH from Escherichia coli (see 7 papers)
    25% identity, 75% coverage of query (64.3 bits)

4ymxA / Q8RCC4 Crystal structure of the substrate binding protein of an amino acid abc transporter (see paper)
    26% identity, 77% coverage of query (63.9 bits)

TC 3.A.1.3.25 / Q9CES5 Glutamine ABC transporter permease and substrate binding protein protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity from Lactococcus lactis subsp. lactis (strain IL1403)
    23% identity, 72% coverage of query (62.4 bits)

4zefA / Q837S0 Crystal structure of substrate binding domain 2 (sbd2) of abc transporter glnpq from enterococcus faecalis
    26% identity, 77% coverage of query (62.0 bits)

2yjpA / Q5F5B5 Crystal structure of the solute receptors for l-cysteine of neisseria gonorrhoeae (see paper)
    25% identity, 83% coverage of query (62.0 bits)

4i62A / A0A0H2ZN67 1.05 angstrom crystal structure of an amino acid abc transporter substrate-binding protein abpa from streptococcus pneumoniae canada mdr_19a bound to l-arginine
    23% identity, 80% coverage of query (62.0 bits)

4g4pA / Q837S0 Crystal structure of glutamine-binding protein from enterococcus faecalis at 1.5 a (see paper)
    25% identity, 72% coverage of query (59.7 bits)

SMc00140 L-lysine ABC transporter, substrate-binding component from Sinorhizobium meliloti 1021
    21% identity, 72% coverage of query (57.8 bits)

GLNH_BACSU / O34563 ABC transporter glutamine-binding protein GlnH from Bacillus subtilis (strain 168) (see paper)
    25% identity, 90% coverage of query (57.4 bits)

BPHYT_RS07735 ABC transporter for L-Arginine, periplasmic substrate-binding component from Burkholderia phytofirmans PsJN
    25% identity, 78% coverage of query (56.2 bits)

8gu1A Crystal structure of putative amino acid binding periplasmic abc transporter protein from candidatus liberibacter asiaticus in complex with pimozide
    24% identity, 75% coverage of query (51.6 bits)

6aalA Crystal structure of putative amino acid binding periplasmic abc transporter protein from candidatus liberibacter asiaticus in complex with arginine
    24% identity, 75% coverage of query (51.6 bits)

6a80A Crystal structure of putative amino acid binding periplasmic abc transporter protein from candidatus liberibacter asiaticus in complex with cystine
    24% identity, 75% coverage of query (51.6 bits)

8gtuA / C6XGT2 Crystal structure of putative amino acid binding periplasmic abc transporter protein from candidatus liberibacter asiaticus in complex with clidinium (see paper)
    24% identity, 75% coverage of query (51.2 bits)

ARTJ_CHLPN / Q9Z869 Probable ABC transporter arginine-binding protein ArtJ from Chlamydia pneumoniae (Chlamydophila pneumoniae) (see 2 papers)
    24% identity, 74% coverage of query (47.4 bits)

3qaxB / Q9Z869 Crystal structure analysis of the cpb0502
    24% identity, 74% coverage of query (47.0 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory