Sites on a Tree

 

Searching for up to 100 curated homologs for AZOBR_RS18070 FitnessBrowser__azobra:AZOBR_RS18070 (223 a.a.)

Found high-coverage hits (≥70%) to 32 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

4ymtC / Q8RCC3 Crystal structure of an amino acid abc transporter complex with arginines (see paper)
    36% identity, 96% coverage of query (142 bits)

4ymwC Crystal structure of an amino acid abc transporter with histidines
    36% identity, 96% coverage of query (142 bits)

TC 3.A.1.3.25 / Q9CES5 Glutamine ABC transporter permease and substrate binding protein protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity from Lactococcus lactis subsp. lactis (strain IL1403)
    33% identity, 91% coverage of query (114 bits)

TC 3.A.1.3.12 / P73544 BgtB aka GLNH aka SLL1270, component of Arginine/lysine/histidine/glutamine porter (see paper)
    35% identity, 83% coverage of query (111 bits)

Build an alignment

Build an alignment for AZOBR_RS18070 and 4 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

AO353_21720 ABC transporter for D-glucosamine, permease component 2 from Pseudomonas fluorescens FW300-N2E3
    29% identity, 93% coverage of query (111 bits)

TC 3.A.1.3.17 / Q8YSA2 Basic amino acid uptake transporter, BgtAB (see paper)
    33% identity, 91% coverage of query (111 bits)

Ac3H11_2554 ABC transporter for L-Histidine, permease component 1 from Acidovorax sp. GW101-3H11
    33% identity, 93% coverage of query (111 bits)

AO356_00470 ABC transporter for D-Glucosamine, permease component 1 from Pseudomonas fluorescens FW300-N2C3
Pf6N2E2_2051 ABC transporter for D-Glucosamine Hydrochloride, permease component 1 from Pseudomonas fluorescens FW300-N2E2
    29% identity, 93% coverage of query (110 bits)

TCYB_BACSU / P42200 L-cystine transport system permease protein TcyB from Bacillus subtilis (strain 168) (see paper)
TC 3.A.1.3.14 / P42200 TcyB (YckJ), component of Uptake system for L-cystine from Bacillus subtilis (see 4 papers)
    36% identity, 78% coverage of query (110 bits)

TcyL / b1918 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
tcyL / P0AFT2 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli (strain K12) (see 3 papers)
TCYL_ECOLI / P0AFT2 L-cystine transport system permease protein TcyL from Escherichia coli (strain K12) (see 5 papers)
TC 3.A.1.3.10 / P0AFT2 Inner membrane amino-acid ABC transporter permease protein YECS CysY aka B1918, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 4 papers)
    32% identity, 86% coverage of query (107 bits)

GlnP / b0810 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
GlnP / P0AEQ6 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.3.2 / P0AEQ6 Glutamine transport system permease protein GlnP aka B0810, component of Three component ABC L-glutamine porter. The basal ATPase activity (ATP hydrolysis in the absence of substrate) is mainly caused by the docking of the closed-unliganded state of GlnH onto the transporter domain of GlnPQ. Unlike glutamine, arginine binds both GlnH domains, but does not trigger their closing. Comparison of the ATPase activity in nanodiscs with glutamine transport in proteoliposomes suggested that the stoichiometry of ATP per substrate is close to two from Escherichia coli (see 5 papers)
    31% identity, 93% coverage of query (106 bits)

GLNM_BACSU / O34671 Probable glutamine ABC transporter permease protein GlnM from Bacillus subtilis (strain 168) (see paper)
    29% identity, 95% coverage of query (101 bits)

Pf1N1B4_3433 ABC transporter for L-Arginine and L-Citrulline, permease component 2 from Pseudomonas fluorescens FW300-N1B4
    31% identity, 90% coverage of query (99.4 bits)

AO356_00475 ABC transporter for D-Glucosamine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    31% identity, 77% coverage of query (99.0 bits)

Pf6N2E2_2052 ABC transporter for D-Glucosamine Hydrochloride, permease component 2 from Pseudomonas fluorescens FW300-N2E2
    31% identity, 77% coverage of query (98.6 bits)

ARTQ_BACSU / P54536 Arginine transport system permease protein ArtQ from Bacillus subtilis (strain 168) (see paper)
    32% identity, 91% coverage of query (98.2 bits)

TC 3.A.1.3.16 / A1VZQ3 PEP1B, component of Uptake system for glutamate and aspartate from Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
    31% identity, 84% coverage of query (97.4 bits)

TC 3.A.1.3.23 / Q9HU29 Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    29% identity, 95% coverage of query (97.1 bits)

AO353_21715 ABC transporter for D-glucosamine, permease component 1 from Pseudomonas fluorescens FW300-N2E3
    29% identity, 83% coverage of query (97.1 bits)

TC 3.A.1.3.11 / O50183 AotP aka AotM aka PA0890, component of Arginine/ornithine (but not lysine) porter from Pseudomonas aeruginosa (see 2 papers)
    32% identity, 88% coverage of query (95.9 bits)

TC 3.A.1.3.6 / P35113 NocM aka ATU6025 aka AGR_PTI_65, component of Nopaline porter from Agrobacterium tumefaciens (see 3 papers)
    28% identity, 98% coverage of query (95.5 bits)

AO356_18710 L-Arginine ABC transporter, permease component 1 from Pseudomonas fluorescens FW300-N2C3
    30% identity, 90% coverage of query (95.1 bits)

AO353_03045 ABC transporter for L-Arginine and L-Citrulline, permease component 1 from Pseudomonas fluorescens FW300-N2E3
    31% identity, 90% coverage of query (95.1 bits)

AO356_09910 ABC transporter for L-Lysine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    29% identity, 95% coverage of query (92.8 bits)

PS417_21735 ABC transporter for L-Arginine, permease component 2 from Pseudomonas simiae WCS417
    32% identity, 70% coverage of query (92.0 bits)

Pf6N2E2_5662 L-Arginine ABC transporter, permease protein AotM from Pseudomonas fluorescens FW300-N2E2
    30% identity, 90% coverage of query (90.5 bits)

TC 3.A.1.3.5 / P35114 OCM1 aka OccM, component of Octopine porter from Agrobacterium tumefaciens (see 2 papers)
    25% identity, 97% coverage of query (89.4 bits)

BPHYT_RS07680 ABC transporter for L-Arginine, permease component 1 from Burkholderia phytofirmans PsJN
    29% identity, 84% coverage of query (89.0 bits)

BPHYT_RS24010 L-histidine ABC transporter, permease component 2 from Burkholderia phytofirmans PsJN
    31% identity, 70% coverage of query (85.1 bits)

TC 3.A.1.3.18 / Q8YPM8 NatG, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (see paper)
    31% identity, 76% coverage of query (84.3 bits)

SMc00138 L-lysine ABC transporter, permease component 2 from Sinorhizobium meliloti 1021
    30% identity, 91% coverage of query (83.6 bits)

TCYL_BACSU / O34315 L-cystine transport system permease protein TcyL from Bacillus subtilis (strain 168) (see 2 papers)
TC 3.A.1.3.13 / O34315 TcyL (YtmL), component of Uptake system for L-cystine (Km=2.5 μM), L-cystathionine, L-djenkolate ( 2-amino-3-[(2-amino-3-hydroxy-3-oxopropyl)sulfanylmethylsulfanyl] propanoic acid), and S-methyl-L-cysteine from Bacillus subtilis (see 4 papers)
    27% identity, 91% coverage of query (83.2 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory