Sites on a Tree

 

Searching for up to 100 curated homologs for AZOBR_RS20010 FitnessBrowser__azobra:AZOBR_RS20010 (365 a.a.)

Found high-coverage hits (≥70%) to 19 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

2hzlB / Q3J1R2 Crystal structures of a sodium-alpha-keto acid binding subunit from a trap transporter in its closed forms (see paper)
    60% identity, 90% coverage of query (422 bits)

TAKP_CERS4 / Q3J1R2 Alpha-keto acid-binding periplasmic protein TakP; Extracytoplasmic solute receptor protein TakP; TRAP transporter alpha-keto acid-binding subunit P; TRAP-T family sorbitol/mannitol transporter, periplasmic binding protein, SmoM from Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter sphaeroides) (see paper)
    58% identity, 97% coverage of query (421 bits)

TC 2.A.56.3.4 / D5ALT6 TRAP dicarboxylate transporter, DctP-2 subunit, component of The 2-ketomonocarboxylate transporter (presented in order of affinity - 2-oxovalerate [highest affinity, KD=0.1 μM], 2-oxoisovalerate, 2-oxobutyrate, 2-oxoisocaproate, 2-oxo-3-methylvalerate, pyruvate [lowest affinity, KD=3 μM]) from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) (see paper)
    58% identity, 97% coverage of query (419 bits)

TMBP_NOSS1 / Q8YSQ6 Monocarboxylate 2-oxoacid-binding periplasmic protein all3028; Extracellular solute-binding protein; Extracytoplasmic solute receptor protein all3028; TRAP transporter monocarboxylate 2-oxoacid-binding subunit P from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
TC 2.A.56.3.3 / Q8YSQ6 Extracellular solute-binding protein, component of The 2-oxo monocarboxylate transporter (Pernil et al., 2010). Transports pyruvate which is inhibited by various 2-ketoacids from Nostoc sp. (strain PCC 7120 / UTEX 2576) (see paper)
    52% identity, 98% coverage of query (405 bits)

4yicA / A0A0J9X284 Crystal structure of a trap transporter solute binding protein (ipr025997) from bordetella bronchiseptica rb50 (bb0280, target efi- 500035) with bound picolinic acid
    53% identity, 90% coverage of query (398 bits)

7ug8B / Q0I6F8 Crystal structure of a solute receptor from synechococcus cc9311 in complex with alpha-ketovaleric and calcium
    51% identity, 89% coverage of query (348 bits)

4petA / Q48AL6 Crystal structure of a trap periplasmic solute binding protein from colwellia psychrerythraea (cps_0129, target efi-510097) with bound calcium and pyruvate (see paper)
    38% identity, 88% coverage of query (241 bits)

5cm6A Crystal structure of a trap periplasmic solute binding protein from pseudoalteromonas atlantica t6c(patl_2292, target efi-510180) with bound sodium and pyruvate
    38% identity, 88% coverage of query (238 bits)

TLBP_THET8 / Q5SK82 Lactate-binding periplasmic protein TTHA0766; ABC transporter, solute-binding protein; Extracytoplasmic solute receptor protein TTHA0766; TRAP transporter lactate-binding subunit P from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
    30% identity, 74% coverage of query (127 bits)

Build an alignment

Build an alignment for AZOBR_RS20010 and 9 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

2zzvA / Q5SK82 Crystal structure of a periplasmic substrate binding protein in complex with calcium and lactate (see paper)
    28% identity, 72% coverage of query (111 bits)

2zzwA Crystal structure of a periplasmic substrate binding protein in complex with zinc and lactate
    28% identity, 72% coverage of query (111 bits)

4xf5A / Q1QZR9 Crystal structure of a trap periplasmic solute binding protein from chromohalobacter salexigens dsm 3043 (csal_0678), target efi-501078, with bound (s)-(+)-2-amino-1-propanol.
    24% identity, 84% coverage of query (77.8 bits)

4uabA Crystal structure of a trap periplasmic solute binding protein from chromohalobacter salexigens dsm 3043 (csal_0678), target efi-501078, with bound ethanolamine
    24% identity, 84% coverage of query (75.5 bits)

TC 2.A.56.3.2 / Q9RBR1 FcbT1, component of Tripartite 4-chlorobenzoate symporter (also binds and may transport 4-bromo-, 4-iodo-, and 4-fluorobenzoate and with a lower affinity, 3-chlorobenzoate, 2-chlorobenzoate, 4-hydroxybenzoate, 3-hydroxybenzoate, and benzoate) (see paper)
    26% identity, 73% coverage of query (72.4 bits)

DCTP2_POLSJ / Q122C7 Solute-binding protein Bpro_4736 from Polaromonas sp. (strain JS666 / ATCC BAA-500) (see paper)
    24% identity, 70% coverage of query (58.9 bits)

4n8yA / A5E8D2 Crystal structure of a trap periplasmic solute binding protein from bradyrhizobium sp. Btai1 b (bbta_0128), target efi-510056 (bbta_0128), complex with alpha/beta-d-galacturonate (see paper)
    24% identity, 72% coverage of query (54.7 bits)

4p8bA / Q0KC03 Crystal structure of a trap periplasmic solute binding protein from ralstonia eutropha h16 (h16_a1328), target efi-510189, with bound (s)-2-hydroxy-2-methyl-3-oxobutanoate ((s)-2-acetolactate) (see paper)
    23% identity, 79% coverage of query (52.8 bits)

PP_1169 D-glucuronate / D-galacturonate TRAP transporter, solute receptor component from Pseudomonas putida KT2440
    24% identity, 77% coverage of query (52.4 bits)

4xfeA / I3UZ52 Crystal structure of a trap periplasmic solute binding protein from pseudomonas putida f1 (pput_1203), target efi-500184, with bound d- glucuronate
    24% identity, 75% coverage of query (49.7 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory