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Searching for up to 100 curated homologs for Ac3H11_2306 FitnessBrowser__acidovorax_3H11:Ac3H11_2306 (222 a.a.)

Found high-coverage hits (≥70%) to 32 curated proteins.

Removed hits that are identical to the query, leaving 31

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Psest_3511 Maleylacetoacetate isomerase (EC 5.2.1.2) from Pseudomonas stutzeri RCH2
    51% identity, 99% coverage of query (216 bits)

SO1671 Maleylacetoacetate isomerase (EC 5.2.1.2) from Shewanella oneidensis MR-1
    50% identity, 99% coverage of query (212 bits)

2v6kA / O86043 Structure of maleyl pyruvate isomerase, a bacterial glutathione-s- transferase in zeta class, in complex with substrate analogue dicarboxyethyl glutathione (see paper)
    49% identity, 99% coverage of query (191 bits)

nagL / O86043 glutathion-dependent maleylpyruvate isomerase (EC 5.2.1.4) from Ralstonia sp. (see paper)
NAGL_RALSP / O86043 Maleylpyruvate isomerase; MPI; Naphthalene degradation protein L; EC 5.2.1.4 from Ralstonia sp. (see 2 papers)
    49% identity, 99% coverage of query (191 bits)

2jl4A Holo structure of maleyl pyruvate isomerase, a bacterial glutathione- s-transferase in zeta class
    49% identity, 99% coverage of query (191 bits)

Q5K5T6 glutathione transferase subunit (EC 2.5.1.18) from Escherichia coli (see 8 papers)
    45% identity, 99% coverage of query (190 bits)

AO356_16835 Maleylacetoacetate isomerase (EC 5.2.1.2) from Pseudomonas fluorescens FW300-N2C3
    42% identity, 99% coverage of query (164 bits)

Pf6N2E2_5292 Maleylacetoacetate isomerase (EC 5.2.1.2) from Pseudomonas fluorescens FW300-N2E2
    42% identity, 99% coverage of query (162 bits)

AO353_15560 Maleylacetoacetate isomerase (EC 5.2.1.2) from Pseudomonas fluorescens FW300-N2E3
    41% identity, 99% coverage of query (156 bits)

1fw1A / O43708 Glutathione transferase zeta/maleylacetoacetate isomerase (see paper)
    41% identity, 98% coverage of query (151 bits)

MAAI2_DROME / Q9VHD2 Probable maleylacetoacetate isomerase 2; MAAI 2; Glutathione S-transferase zeta 2; EC 5.2.1.2; EC 2.5.1.18 from Drosophila melanogaster (Fruit fly) (see paper)
    39% identity, 98% coverage of query (150 bits)

GSTZ1 / O43708 maleylacetoacetate isomerase/glutathione transferase ζ monomer (EC 2.5.1.18; EC 5.2.1.2) from Homo sapiens (see 11 papers)
MAAI_HUMAN / O43708 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Homo sapiens (Human) (see 3 papers)
O43708 glutathione transferase (EC 2.5.1.18); maleylacetoacetate isomerase (EC 5.2.1.2) from Homo sapiens (see 8 papers)
    40% identity, 98% coverage of query (149 bits)

MAAI_RAT / P57113 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Rattus norvegicus (Rat) (see paper)
    40% identity, 99% coverage of query (149 bits)

2cz2A / Q9WVL0 Crystal structure of glutathione transferase zeta 1-1 (maleylacetoacetate isomerase) from mus musculus (form-1 crystal)
    39% identity, 99% coverage of query (148 bits)

Q9WVL0 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Mus musculus (Mouse)
    39% identity, 99% coverage of query (148 bits)

4kdyA / B8J605 Crystal structure of maleylacetoacetate isomerase from anaeromyxobacter dehalogenans 2cp-1, target efi-507175, with bound gsh in the active site
    42% identity, 99% coverage of query (140 bits)

4kaeA Crystal structure of maleylacetoacetate isomerase from anaeromyxobacter dehalogenans 2cp-1, target efi-507175, with bound dicarboxyethyl glutathione and citrate in the active site
    42% identity, 99% coverage of query (140 bits)

MAAI1_DROME / Q9VHD3 Probable maleylacetoacetate isomerase 1; MAAI 1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Drosophila melanogaster (Fruit fly) (see paper)
    35% identity, 98% coverage of query (136 bits)

GSTZ1_ARATH / Q9ZVQ3 Glutathione S-transferase Z1; AtGSTZ1; GST class-zeta member 1; Glutathione S-transferase 18; Maleylacetone isomerase; MAI; EC 2.5.1.18; EC 5.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    38% identity, 99% coverage of query (134 bits)

3n5oA / D2YW48 Crystal structure of putative glutathione transferase from coccidioides immitis bound to glutathione (see paper)
    35% identity, 96% coverage of query (125 bits)

D2YW48 Probable glutathione S-transferase; EC 2.5.1.18 from Coccidioides immitis (strain RS) (Valley fever fungus)
    35% identity, 96% coverage of query (125 bits)

MAIA_ASPFU / Q4WHT7 Maleylacetoacetate isomerase maiA; EC 5.2.1.2 from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see 3 papers)
    36% identity, 87% coverage of query (120 bits)

maiA / O43123 maleylacetoacetate isomerase (EC 5.2.1.2) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (see paper)
MAAI_EMENI / O43123 Maleylacetoacetate isomerase; MAAI; EC 5.2.1.2 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
O43123 maleylacetoacetate isomerase (EC 5.2.1.2) from Aspergillus nidulans (see paper)
GI|2832725 maleylacetoacetate isomerase; EC 5.2.1.2 from Emericella nidulans (see paper)
maiA / CAA05041.1 maleylacetoacetate isomerase from Aspergillus nidulans (see paper)
maiA / CAA05044.1 maleylacetoacetate isomerase from Aspergillus nidulans (see paper)
    35% identity, 91% coverage of query (119 bits)

4pxoA / C5ATQ9 Crystal structure of maleylacetoacetate isomerase from methylobacteriu extorquens am1 with bound malonate and gsh (target efi-507068)
    34% identity, 96% coverage of query (108 bits)

O04437 glutathione transferase (EC 2.5.1.18) from Triticum aestivum (see paper)
    31% identity, 98% coverage of query (89.4 bits)

Build an alignment

Build an alignment for Ac3H11_2306 and 25 homologs with ≥ 30% identity

Select sequences

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Additional hits (identity < 30%)

Q8GTB9 glutathione transferase (EC 2.5.1.18) from Triticum aestivum (see paper)
    25% identity, 99% coverage of query (50.1 bits)

A0A4Y5R032 glutathione transferase (EC 2.5.1.18) from Salix babylonica (see paper)
    30% identity, 71% coverage of query (49.7 bits)

A4LAG9 glutathione transferase (EC 2.5.1.18) from Vitis vinifera (see paper)
    29% identity, 74% coverage of query (48.5 bits)

7dw4A / A0A4Y5R032 Crystal structure of a glutathione s-transferase mutant sbgstu6(i55t) from salix babylonica in complex with glutathione
    30% identity, 71% coverage of query (48.1 bits)

Pog / b3229 stringent starvation protein A from Escherichia coli K-12 substr. MG1655 (see 8 papers)
TC 1.A.12.3.1 / P0ACA3 The bacterial CLIC homologue, stringent starvation protein A, SspA (212 aas; 0 TMSs) [N-terminal Trx domain; C-terminal glutathione S-transferase (GST) domain] from Escherichia coli (strain K12) (see 5 papers)
sspA stringent starvation protein A from Escherichia coli K12 (see 5 papers)
    27% identity, 82% coverage of query (47.0 bits)

3touA / Q8XVV6 Crystal structure of glutathione transferase (target efi-501058) from ralstonia solanacearum gmi1000 with gsh bound
    25% identity, 97% coverage of query (43.5 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory