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Searching for up to 100 curated homologs for Ac3H11_3993 FitnessBrowser__acidovorax_3H11:Ac3H11_3993 (394 a.a.)

Found high-coverage hits (≥70%) to 38 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

pobA / AAA73519.1 4-hydroxybenzoate hydroxylase from Rhizobium leguminosarum (see paper)
    67% identity, 99% coverage of query (547 bits)

1pbcA Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring
    65% identity, 99% coverage of query (534 bits)

pobA / P00438 p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens (see 2 papers)
PHHY_PSEFL / P00438 p-hydroxybenzoate hydroxylase; PHBH; PHBHase; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 from Pseudomonas fluorescens (see 13 papers)
    65% identity, 99% coverage of query (533 bits)

1bf3A P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 replaced by ser (c116s) and arg 42 replaced by lys (r42k), in complex with fad and 4-hydroxybenzoic acid
    65% identity, 99% coverage of query (533 bits)

2phhA The coenzyme analogue adenosine 5-diphosphoribose displaces fad in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation
    65% identity, 99% coverage of query (533 bits)

1pdhA Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified fad present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin
    65% identity, 99% coverage of query (533 bits)

PHHY_PSEAE / P20586 p-hydroxybenzoate hydroxylase; PHBH; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 7 papers)
1d7lA / P20586 Structure-function correlations of the reaction of reduced nicotinamide analogs with p-hydroxybenzoate hydroxylase substituted with a series of 8-substituted flavins (see paper)
    65% identity, 99% coverage of query (532 bits)

1iusA P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate at ph 5.0
    65% identity, 99% coverage of query (532 bits)

1dodA The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis
    65% identity, 99% coverage of query (532 bits)

1k0lA Pseudomonas aeruginosa phbh r220q free of p-ohb
    65% identity, 99% coverage of query (530 bits)

1k0jA Pseudomonas aeruginosa phbh r220q in complex with NADPH and free of p- ohb
    65% identity, 99% coverage of query (530 bits)

1ykjB A45g p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bound
    65% identity, 99% coverage of query (523 bits)

RR42_RS21940 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Cupriavidus basilensis FW507-4G11
    68% identity, 99% coverage of query (517 bits)

Pf6N2E2_2914 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Pseudomonas fluorescens FW300-N2E2
    63% identity, 99% coverage of query (509 bits)

pobA / Q9R9T1 p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas putida (see paper)
Q9R9T1 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Pseudomonas putida (see paper)
    63% identity, 99% coverage of query (507 bits)

6dllB / Q88H28 2.2 angstrom resolution crystal structure of p-hydroxybenzoate hydroxylase from pseudomonas putida in complex with fad. (see paper)
    61% identity, 99% coverage of query (497 bits)

pobA / F2JUE7 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1) (see paper)
    57% identity, 99% coverage of query (465 bits)

PRAI_PAESP / C4TP09 4-hydroxybenzoate 3-monooxygenase (NAD(P)H); 4-hydroxybenzoate 3-hydroxylase; 4HB 3-hydroxylase; EC 1.14.13.33 from Paenibacillus sp. (see paper)
praI / BAH79107.1 4-hydroxybenzoate 3-hydroxylase from Paenibacillus sp. JJ-1b (see paper)
    54% identity, 99% coverage of query (420 bits)

7on9A / C4TP09 Crystal structure of para-hydroxybenzoate-3-hydroxylase prai (see paper)
    54% identity, 99% coverage of query (417 bits)

8jqoA Protocatecuate hydroxylase from xylophilus ampelinus complexed with imidazole (see paper)
    47% identity, 99% coverage of query (382 bits)

8jqoD Protocatecuate hydroxylase from xylophilus ampelinus complexed with imidazole (see paper)
    39% identity, 99% coverage of query (273 bits)

Build an alignment

Build an alignment for Ac3H11_3993 and 21 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

6ui5A / Q1MX79 Tmn9 in complex with cofactor fad
    27% identity, 82% coverage of query (65.1 bits)

7yj0D Structural basis of oxepinone formation by a flavin-monooxygenase vibo
    24% identity, 77% coverage of query (63.9 bits)

nat2 / F8QPH2 ansamycin ring hydroxylase from Streptomyces sp. CS (see 5 papers)
    26% identity, 77% coverage of query (62.4 bits)

rif19 / Q9AE02 rifamycin ring hydroxylase from Amycolatopsis mediterranei (see 4 papers)
    26% identity, 78% coverage of query (59.3 bits)

ROX_NOCFA / Q5YTV5 Rifampicin monooxygenase; RIFMO; EC 1.14.13.211 from Nocardia farcinica (strain IFM 10152) (see 3 papers)
Q5YTV5 rifampicin monooxygenase (EC 1.14.13.211) from Nocardia farcinica (see paper)
    30% identity, 76% coverage of query (55.5 bits)

5kowA / Q5YTV5 Structure of rifampicin monooxygenase (see paper)
    30% identity, 76% coverage of query (55.5 bits)

6c7sA Structure of rifampicin monooxygenase with product bound
    30% identity, 76% coverage of query (55.5 bits)

5koxA Structure of rifampicin monooxygenase complexed with rifampicin
    30% identity, 76% coverage of query (55.5 bits)

Cgl1158 / Q8NR94 resorcinol 4-hydroxylase (NADPH) (EC 1.14.13.219) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see 2 papers)
RES4H_CORGL / Q8NR94 NADPH-dependent resorcinol 4-hydroxylase; Resorcinol hydroxylase; EC 1.14.13.219 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
    25% identity, 75% coverage of query (55.1 bits)

stiL / CAD19095.1 cytochrome P450 dependent monooxygenase from Stigmatella aurantiaca (see paper)
    23% identity, 78% coverage of query (54.3 bits)

6u0sA Crystal structure of the flavin-dependent monooxygenase piee in complex with fad and substrate
    23% identity, 84% coverage of query (52.8 bits)

6u0pC / W0C4C9 Crystal structure of piee, the flavin-dependent monooxygenase involved in the biosynthesis of piericidin a1 (see paper)
    23% identity, 84% coverage of query (52.8 bits)

8fhjC Crystal structure of a fad monooxygenease from methylocystis sp. Strain sb2 (see paper)
    27% identity, 83% coverage of query (52.4 bits)

TERD_ASPTN / Q0D1P2 FAD-dependent monooxygenase terD; Terrein biosynthesis cluster protein terD; EC 1.-.-.- from Aspergillus terreus (strain NIH 2624 / FGSC A1156) (see 13 papers)
    22% identity, 77% coverage of query (52.4 bits)

mtmOII / Q194R1 MtmOII from Streptomyces argillaceus (see 3 papers)
    23% identity, 86% coverage of query (46.6 bits)

elmG / Q9L4Y1 tetracenomycin B2 oxygenase (EC 1.14.13.200) from Streptomyces olivaceus (see 6 papers)
ELMG_STROV / Q9L4Y1 Tetracenomycin B2 monooxygenase-dioxygenase; Tetracenomycin B2 oxygenase; Tcm B2 oxygenase; EC 1.14.13.200 from Streptomyces olivaceus (see 2 papers)
Q9L4Y1 tetracenomycin A2 monooxygenase-dioxygenase (EC 1.14.13.200) from Streptomyces olivaceus (see 2 papers)
    23% identity, 82% coverage of query (46.2 bits)

2qa1A / Q93LY7 Crystal structure of pgae, an aromatic hydroxylase involved in angucycline biosynthesis (see paper)
    24% identity, 81% coverage of query (43.9 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory