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Searching for up to 100 curated homologs for BPHYT_RS16025 FitnessBrowser__BFirm:BPHYT_RS16025 (212 a.a.)

Found high-coverage hits (≥70%) to 26 curated proteins.

Removed hits that are identical to the query, leaving 25

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Q63WP3 arylformamidase (EC 3.5.1.9) from Burkholderia pseudomallei (see paper)
    80% identity, 100% coverage of query (342 bits)

KYNB_BURCJ / B4E9I9 Kynurenine formamidase; KFA; KFase; Arylformamidase; N-formylkynurenine formamidase; FKF; EC 3.5.1.9 from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)) (see paper)
B4E9I9 arylformamidase (EC 3.5.1.9) from Burkholderia cenocepacia (see paper)
    81% identity, 99% coverage of query (339 bits)

4cogA / B4E9I9 Crystal structure of kynurenine formamidase from burkholderia cenocepacia (see paper)
    81% identity, 98% coverage of query (338 bits)

KYNB_CUPMC / P0C8P4 Kynurenine formamidase; KFA; KFase; Arylformamidase; N-formylkynurenine formamidase; FKF; EC 3.5.1.9 from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans) (see 2 papers)
kynB kynurenine formamidase; EC 3.5.1.9 from Ralstonia metallidurans CH34 (see 2 papers)
    71% identity, 97% coverage of query (298 bits)

KYNB_PSEAE / Q9I234 Kynurenine formamidase; KFA; KFase; Arylformamidase; N-formylkynurenine formamidase; FKF; EC 3.5.1.9 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
Q9I234 arylformamidase (EC 3.5.1.9) from Pseudomonas aeruginosa (see paper)
kynB kynurenine formamidase; EC 3.5.1.9 from Pseudomonas aeruginosa (see 2 papers)
    68% identity, 98% coverage of query (289 bits)

4cobA / Q9I234 Crystal structure kynurenine formamidase from pseudomonas aeruginosa (see paper)
    69% identity, 97% coverage of query (288 bits)

PS417_23710 Kynurenine formamidase, bacterial (EC 3.5.1.9) from Pseudomonas simiae WCS417
    67% identity, 98% coverage of query (281 bits)

PfGW456L13_4293 Kynurenine formamidase, bacterial (EC 3.5.1.9) from Pseudomonas fluorescens GW456-L13
    65% identity, 99% coverage of query (278 bits)

AO353_05975 Kynurenine formamidase, bacterial (EC 3.5.1.9) from Pseudomonas fluorescens FW300-N2E3
    67% identity, 96% coverage of query (277 bits)

KYNB_PSEFL / Q84HF4 Kynurenine formamidase; KFA; KFase; Arylformamidase; N-formylkynurenine formamidase; FKF; EC 3.5.1.9 from Pseudomonas fluorescens (see paper)
    60% identity, 99% coverage of query (244 bits)

KYNB_BACCR / Q81CK1 Kynurenine formamidase; KFA; KFase; Arylformamidase; N-formylkynurenine formamidase; FKF; EC 3.5.1.9 from Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711) (see paper)
kynB kynurenine formamidase; EC 3.5.1.9 from Bacillus cereus ATCC 14579 (see 2 papers)
    40% identity, 95% coverage of query (160 bits)

KYNB_BACAN / Q81PP9 Kynurenine formamidase; KFA; KFase; Arylformamidase; N-formylkynurenine formamidase; FKF; EC 3.5.1.9 from Bacillus anthracis (see paper)
Q81PP9 arylformamidase (EC 3.5.1.9) from Bacillus anthracis (see paper)
    40% identity, 95% coverage of query (158 bits)

4co9A / Q81PP9 Crystal structure of kynurenine formamidase from bacillus anthracis (see paper)
    40% identity, 95% coverage of query (157 bits)

4cz1A Crystal structure of kynurenine formamidase from bacillus anthracis complexed with 2-aminoacetophenone.
    40% identity, 95% coverage of query (157 bits)

Build an alignment

Build an alignment for BPHYT_RS16025 and 14 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

1r61A / P84132 The structure of predicted metal-dependent hydrolase from bacillus stearothermophilus
    28% identity, 95% coverage of query (80.5 bits)

8hmoA / A0A0H4P0S7 Crystal structure of metal-dependent hydrolase complexed with manganese from bacillus smithii
    27% identity, 92% coverage of query (78.2 bits)

CYL2_ORYSJ / Q67WE2 Cyclase-like protein 2; OsCYL2 from Oryza sativa subsp. japonica (Rice) (see paper)
    31% identity, 95% coverage of query (72.4 bits)

CYL3_ORYSJ / Q0J6H8 Cyclase-like protein 3; OsCYL3 from Oryza sativa subsp. japonica (Rice) (see paper)
    27% identity, 94% coverage of query (69.7 bits)

CYL1_ARATH / Q93V74 Cyclase-like protein 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    29% identity, 95% coverage of query (62.8 bits)

CYL2_ARATH / Q94JT5 Cyclase-like protein 2 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    29% identity, 95% coverage of query (61.2 bits)

CYL4_ORYSJ / Q6YX89 Cyclase-like protein 4; OsCYL4 from Oryza sativa subsp. japonica (Rice) (see paper)
    29% identity, 94% coverage of query (54.7 bits)

CYL1_ORYSJ / Q6ZIF9 Cyclase-like protein 1; OsCYL1 from Oryza sativa subsp. japonica (Rice) (see paper)
    27% identity, 95% coverage of query (53.9 bits)

dpsY / O68500 12-deoxyaklanoate synthase from Streptomyces peucetius (see 2 papers)
    28% identity, 80% coverage of query (50.8 bits)

stfY / CAJ42324.1 cyclase from Streptomyces steffisburgensis (see paper)
    27% identity, 80% coverage of query (48.1 bits)

mtmY / Q194Q6 MtmY from Streptomyces argillaceus (see 3 papers)
    28% identity, 80% coverage of query (45.1 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory