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Searching for up to 100 curated homologs for BPHYT_RS16115 FitnessBrowser__BFirm:BPHYT_RS16115 (440 a.a.)

Found high-coverage hits (≥70%) to 34 curated proteins.

Removed hits that are identical to the query, leaving 33

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

mtlE / Q4KC57 polyol ABC-type transporter periplasmic substrate-binding protein from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) (see paper)
    60% identity, 100% coverage of query (549 bits)

4ryaA / B9JRF8 Crystal structure of abc transporter solute binding protein avi_3567 from agrobacterium vitis s4, target efi-510645, with bound d-mannitol
    61% identity, 94% coverage of query (545 bits)

PS417_12715 ABC transporter for D-Mannitol and D-Sorbitol, periplasmic substrate-binding protein from Pseudomonas simiae WCS417
    61% identity, 99% coverage of query (543 bits)

AO356_00025 ABC transporter for D-Sorbitol, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2C3
    61% identity, 96% coverage of query (542 bits)

TC 3.A.1.1.49 / O30491 MtlE, component of The polyol (mannitol, glucitol (sorbitol), arabitol (arabinitol; lyxitol)) uptake porter, MtlEFGK from Pseudomonas fluorescens
    61% identity, 99% coverage of query (541 bits)

Pf6N2E2_1963 ABC transporter for D-sorbitol, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    60% identity, 96% coverage of query (539 bits)

MANBP_PSEAE / Q9I1E0 Mannitol-binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    60% identity, 94% coverage of query (530 bits)

Ac3H11_2944 ABC transporter for D-Sorbitol, periplasmic substrate-binding component from Acidovorax sp. GW101-3H11
    59% identity, 94% coverage of query (511 bits)

TC 3.A.1.1.5 / O30831 SmoE, component of Hexitol (glucitol; mannitol) porter from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
    57% identity, 98% coverage of query (499 bits)

PGA1_c13210 ABC transporter for D-Sorbitol, periplasmic substrate-binding component from Phaeobacter inhibens BS107
    57% identity, 99% coverage of query (495 bits)

Build an alignment

Build an alignment for BPHYT_RS16115 and 10 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Additional hits (identity < 30%)

HSERO_RS17000 ABC transporter for D-sorbitol/xylitol, substrate-binding component from Herbaspirillum seropedicae SmR1
    29% identity, 74% coverage of query (111 bits)

5iaiA / B9JM84 Crystal structure of abc transporter solute binding protein arad_9887 from agrobacterium radiobacter k84, target efi-510945 in complex with ribitol
    27% identity, 90% coverage of query (107 bits)

TC 3.A.1.1.7 / O51923 MalE aka PF1739, component of Maltose/trehalose porter (see paper)
    27% identity, 93% coverage of query (102 bits)

1eu8A / Q7LYW7 Structure of trehalose maltose binding protein from thermococcus litoralis (see paper)
    27% identity, 93% coverage of query (102 bits)

MALE_THELN / Q7LYW7 Trehalose/maltose-binding protein MalE; TMBP from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see 4 papers)
    26% identity, 93% coverage of query (100 bits)

6dtqA / G4XU73 Maltose bound t. Maritima male3 (see paper)
    27% identity, 73% coverage of query (91.3 bits)

TC 3.A.1.1.41 / G4FGN8 Extracellular solute-binding protein family 1, component of Trehalose porter. Also binds sucrose (Boucher and Noll, 2011). Induced by glucose and trehalose. Directly regulated by trehalose-responsive regulator TreR from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    27% identity, 73% coverage of query (90.9 bits)

smoF / A9CEY9 3-(6-sulfo-α-D-quinovosyl)-sn-glycerol binding protein from Agrobacterium fabrum (strain C58 / ATCC 33970) (see paper)
SMOF_AGRFC / A9CEY9 Sulfoquinovosyl glycerol-binding protein SmoF; SQGro-binding protein SmoF; SQ monooxygenase cluster protein F from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see 2 papers)
    25% identity, 93% coverage of query (84.3 bits)

7qhvAAA / A9CEY9 7qhvAAA (see paper)
    25% identity, 88% coverage of query (83.2 bits)

7ofyA Crystal structure of sq binding protein from agrobacterium tumefaciens in complex with sulfoquinovosyl glycerol (sqgro)
    25% identity, 88% coverage of query (82.4 bits)

7yzsAAA Sulfoquinovosyl binding protein
    25% identity, 88% coverage of query (82.4 bits)

7yzuA Crystal structure of the sulfoquinovosyl binding protein smof complexed with sqme
    25% identity, 88% coverage of query (82.0 bits)

SM_b21221 ABC transporter for D-Glucosamine, periplasmic substrate-binding protein from Sinorhizobium meliloti 1021
    23% identity, 87% coverage of query (72.0 bits)

Dshi_0547 ABC transporter for Xylitol, periplasmic substrate-binding component from Dinoroseobacter shibae DFL-12
    24% identity, 93% coverage of query (69.3 bits)

TC 3.A.1.1.16 / P58300 MalE aka PF1938, component of Maltooligosaccharide porter (Maltose is not a substrate, but maltotriose is.) from Pyrococcus furiosus (see paper)
    23% identity, 73% coverage of query (68.6 bits)

5f7vA / Q8YAE9 Abc substrate-binding protein lmo0181 from listeria monocytogenes in complex with cycloalternan (see paper)
    26% identity, 73% coverage of query (68.2 bits)

1eljA / P58300 The crystal structure of liganded maltodextrin-binding protein from pyrococcus furiosus (see paper)
    22% identity, 73% coverage of query (65.9 bits)

TC 3.A.1.1.44 / Q7AKP1 Putative maltose-binding protein, component of MalEFG (K unknown), involved in maltose and maltodextrin uptake from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    25% identity, 86% coverage of query (60.5 bits)

YcjN / b1310 putative ABC transporter periplasmic binding protein YcjN from Escherichia coli K-12 substr. MG1655 (see 4 papers)
TC 3.A.1.1.46 / P76042 Putative ABC transporter periplasmic-binding protein YcjN, component of Probable glucoside uptake porter, YcjNOPV from Escherichia coli (strain K12)
    23% identity, 90% coverage of query (59.7 bits)

7ehpA Chitin oligosaccharide binding protein (see paper)
    22% identity, 87% coverage of query (57.4 bits)

MSME_STRMU / Q00749 Multiple sugar-binding protein from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see paper)
TC 3.A.1.1.28 / Q00749 MsmE aka SMU.878, component of The raffinose/stachyose transporter, MsmEFGK (MalK (3.A.1.1.27) can probably substitute for MsmK; Webb et al., 2008). This system may also transport melibiose, isomaltotriose and sucrose as well as isomaltosaccharides from Streptococcus mutans (see 2 papers)
    24% identity, 75% coverage of query (52.4 bits)

TC 3.A.1.1.33 / Q8KN19 Putative sugar transporter sugar-binding protein, component of The N,N'-diacetylchitobiose uptake transporter, DasABC/MsiK (MsiK energizes several ABC transporters (see 3.A.1.1.23)) from Streptomyces sp. 139 (see 2 papers)
    25% identity, 71% coverage of query (51.6 bits)

SM_b21103 ABC transporter for L-Fucose, periplasmic substrate-binding protein from Sinorhizobium meliloti 1021
    23% identity, 84% coverage of query (48.5 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory