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Searching for up to 100 curated homologs for BPHYT_RS34230 FitnessBrowser__BFirm:BPHYT_RS34230 (431 a.a.)

Found high-coverage hits (≥70%) to 12 curated proteins.

Removed hits that are identical to the query, leaving 11

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

lgnE / BAM68208.1 D-idonate dehydratase lgnE from Paracoccus laeviglucosivorans (see 2 papers)
    36% identity, 91% coverage of query (216 bits)

SMc02776 D-arabinoate dehydratase (EC 4.2.1.5); also involved in L-fucose catabolism from Sinorhizobium meliloti 1021
    36% identity, 92% coverage of query (205 bits)

C785_RS13685 / J3DH42 D-arabinonate dehydratase (EC 4.2.1.5) from Herbaspirillum sp. CF444 (see paper)
    35% identity, 91% coverage of query (201 bits)

suyB / Q1QWP0 R-sulfolactate sulfo-lyase B subunit (EC 4.4.1.24) from Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) (see paper)
SUYB_CHRSD / Q1QWP0 (2R)-sulfolactate sulfo-lyase subunit beta; Sulfolactate sulfo-lyase B; EC 4.4.1.24 from Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) (see paper)
    35% identity, 92% coverage of query (170 bits)

HSERO_RS15800 Galactarate dehydratase (EC 4.2.1.42) from Herbaspirillum seropedicae SmR1
    32% identity, 92% coverage of query (161 bits)

suyB / Q58Y43 3-sulfolactate sulfo-lyase large subunit (EC 4.4.1.24) from Paracoccus pantotrophus (see paper)
SUYB_PARPN / Q58Y43 (2R)-sulfolactate sulfo-lyase subunit beta; Sulfolactate sulfo-lyase B; EC 4.4.1.24 from Paracoccus pantotrophus (Thiosphaera pantotropha) (see paper)
    31% identity, 94% coverage of query (155 bits)

Build an alignment

Build an alignment for BPHYT_RS34230 and 6 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

YgjW / b3091 D-altronate dehydratase (EC 4.2.1.7) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
uxaA / P42604 D-altronate dehydratase (EC 4.2.1.7) from Escherichia coli (strain K12) (see 3 papers)
UXAA_ECOLI / P42604 Altronate dehydratase; D-altronate hydro-lyase; EC 4.2.1.7 from Escherichia coli (strain K12) (see 2 papers)
    29% identity, 93% coverage of query (151 bits)

PS417_04210 Galactarate dehydratase (EC 4.2.1.42) from Pseudomonas simiae WCS417
    31% identity, 89% coverage of query (148 bits)

YhaG / b3128 (EC 4.2.1.42) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
garD / P39829 galactarate dehydratase (EC 4.2.1.42) from Escherichia coli (strain K12) (see 5 papers)
GARD_ECOLI / P39829 Galactarate dehydratase (L-threo-forming); GalcD; EC 4.2.1.42 from Escherichia coli (strain K12) (see 3 papers)
    30% identity, 92% coverage of query (142 bits)

CA265_RS19875 Altronate dehydratase (EC 4.2.1.7) from Pedobacter sp. GW460-11-11-14-LB5
    29% identity, 76% coverage of query (135 bits)

6u7lA / P39829 2.75 angstrom crystal structure of galactarate dehydratase from escherichia coli. (see paper)
    28% identity, 92% coverage of query (123 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory