Sites on a Tree

 

Searching for up to 100 curated homologs for BWI76_RS08970 BWI76_RS08970 glutamine ABC transporter permease GlnP (219 a.a.)

Found high-coverage hits (≥70%) to 34 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

GlnP / b0810 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
GlnP / P0AEQ6 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.3.2 / P0AEQ6 Glutamine transport system permease protein GlnP aka B0810, component of Three component ABC L-glutamine porter. The basal ATPase activity (ATP hydrolysis in the absence of substrate) is mainly caused by the docking of the closed-unliganded state of GlnH onto the transporter domain of GlnPQ. Unlike glutamine, arginine binds both GlnH domains, but does not trigger their closing. Comparison of the ATPase activity in nanodiscs with glutamine transport in proteoliposomes suggested that the stoichiometry of ATP per substrate is close to two from Escherichia coli (see 5 papers)
    94% identity, 100% coverage of query (409 bits)

TC 3.A.1.3.17 / Q8YSA2 Basic amino acid uptake transporter, BgtAB (see paper)
    46% identity, 92% coverage of query (167 bits)

Ac3H11_2554 ABC transporter for L-Histidine, permease component 1 from Acidovorax sp. GW101-3H11
    39% identity, 99% coverage of query (155 bits)

TC 3.A.1.3.12 / P73544 BgtB aka GLNH aka SLL1270, component of Arginine/lysine/histidine/glutamine porter (see paper)
    43% identity, 93% coverage of query (153 bits)

TC 3.A.1.3.25 / Q9CES5 Glutamine ABC transporter permease and substrate binding protein protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity from Lactococcus lactis subsp. lactis (strain IL1403)
    35% identity, 91% coverage of query (142 bits)

4ymtC / Q8RCC3 Crystal structure of an amino acid abc transporter complex with arginines (see paper)
    36% identity, 99% coverage of query (140 bits)

AO356_00475 ABC transporter for D-Glucosamine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    38% identity, 99% coverage of query (139 bits)

4ymwC Crystal structure of an amino acid abc transporter with histidines
    36% identity, 99% coverage of query (139 bits)

TCYL_BACSU / O34315 L-cystine transport system permease protein TcyL from Bacillus subtilis (strain 168) (see 2 papers)
TC 3.A.1.3.13 / O34315 TcyL (YtmL), component of Uptake system for L-cystine (Km=2.5 μM), L-cystathionine, L-djenkolate ( 2-amino-3-[(2-amino-3-hydroxy-3-oxopropyl)sulfanylmethylsulfanyl] propanoic acid), and S-methyl-L-cysteine from Bacillus subtilis (see 4 papers)
    35% identity, 98% coverage of query (138 bits)

Pf6N2E2_2052 ABC transporter for D-Glucosamine Hydrochloride, permease component 2 from Pseudomonas fluorescens FW300-N2E2
    37% identity, 99% coverage of query (138 bits)

ARTQ_BACSU / P54536 Arginine transport system permease protein ArtQ from Bacillus subtilis (strain 168) (see paper)
    36% identity, 99% coverage of query (137 bits)

TcyL / b1918 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
tcyL / P0AFT2 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli (strain K12) (see 3 papers)
TCYL_ECOLI / P0AFT2 L-cystine transport system permease protein TcyL from Escherichia coli (strain K12) (see 5 papers)
TC 3.A.1.3.10 / P0AFT2 Inner membrane amino-acid ABC transporter permease protein YECS CysY aka B1918, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 4 papers)
    37% identity, 95% coverage of query (136 bits)

TCYB_BACSU / P42200 L-cystine transport system permease protein TcyB from Bacillus subtilis (strain 168) (see paper)
TC 3.A.1.3.14 / P42200 TcyB (YckJ), component of Uptake system for L-cystine from Bacillus subtilis (see 4 papers)
    41% identity, 94% coverage of query (135 bits)

AO353_21715 ABC transporter for D-glucosamine, permease component 1 from Pseudomonas fluorescens FW300-N2E3
    37% identity, 99% coverage of query (134 bits)

AO356_09910 ABC transporter for L-Lysine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    34% identity, 98% coverage of query (124 bits)

TC 3.A.1.3.23 / Q9HU29 Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    32% identity, 98% coverage of query (124 bits)

YXEN_BACSU / P54953 Probable amino-acid permease protein YxeN from Bacillus subtilis (strain 168) (see paper)
    32% identity, 98% coverage of query (122 bits)

TC 3.A.1.3.19 / Q88NY4 PP1069, component of Acidic amino acid uptake porter, AatJMQP from Pseudomonas putida (strain KT2440) (see paper)
    33% identity, 100% coverage of query (121 bits)

TC 3.A.1.3.22 / Q9I404 Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    33% identity, 98% coverage of query (120 bits)

PfGW456L13_4772 ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 2 from Pseudomonas fluorescens GW456-L13
    33% identity, 100% coverage of query (120 bits)

Pf1N1B4_773 ABC transporter for L-asparagine and L-glutamate, permease subunit 2 from Pseudomonas fluorescens FW300-N1B4
    32% identity, 100% coverage of query (117 bits)

AO353_16280 ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 1 from Pseudomonas fluorescens FW300-N2E3
    33% identity, 100% coverage of query (116 bits)

AO353_21720 ABC transporter for D-glucosamine, permease component 2 from Pseudomonas fluorescens FW300-N2E3
    34% identity, 96% coverage of query (113 bits)

TC 3.A.1.3.5 / P35114 OCM1 aka OccM, component of Octopine porter from Agrobacterium tumefaciens (see 2 papers)
    32% identity, 97% coverage of query (112 bits)

Pf6N2E2_2959 ABC transporter for L-Lysine, permease component 1 from Pseudomonas fluorescens FW300-N2E2
    36% identity, 74% coverage of query (112 bits)

AO356_05500 ABC transporter for L-Lysine, permease component 1 from Pseudomonas fluorescens FW300-N2C3
    36% identity, 74% coverage of query (112 bits)

AO356_00470 ABC transporter for D-Glucosamine, permease component 1 from Pseudomonas fluorescens FW300-N2C3
Pf6N2E2_2051 ABC transporter for D-Glucosamine Hydrochloride, permease component 1 from Pseudomonas fluorescens FW300-N2E2
    34% identity, 96% coverage of query (110 bits)

TC 3.A.1.3.16 / A1VZQ3 PEP1B, component of Uptake system for glutamate and aspartate from Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
    30% identity, 93% coverage of query (108 bits)

Build an alignment

Build an alignment for BWI76_RS08970 and 28 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

GLNM_BACSU / O34671 Probable glutamine ABC transporter permease protein GlnM from Bacillus subtilis (strain 168) (see paper)
    29% identity, 98% coverage of query (107 bits)

TC 3.A.1.3.18 / Q8YPM8 NatG, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (see paper)
    32% identity, 90% coverage of query (107 bits)

GLNP_BACSU / O34606 Probable glutamine ABC transporter permease protein GlnP from Bacillus subtilis (strain 168) (see paper)
    34% identity, 91% coverage of query (106 bits)

TC 3.A.1.3.6 / P35113 NocM aka ATU6025 aka AGR_PTI_65, component of Nopaline porter from Agrobacterium tumefaciens (see 3 papers)
    31% identity, 93% coverage of query (103 bits)

GltK / b0653 glutamate/aspartate ABC transporter membrane subunit GltK (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
GltK / P0AER5 glutamate/aspartate ABC transporter membrane subunit GltK (EC 7.4.2.1) from Escherichia coli (strain K12) (see 3 papers)
GLTK_ECOLI / P0AER5 Glutamate/aspartate import permease protein GltK from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.3.4 / P0AER5 Glutamate/aspartate transport system permease protein GltK aka B0653, component of Glutamate/aspartate porter from Escherichia coli (see 4 papers)
    30% identity, 98% coverage of query (102 bits)

BPHYT_RS24010 L-histidine ABC transporter, permease component 2 from Burkholderia phytofirmans PsJN
    31% identity, 99% coverage of query (101 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory