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Searching for up to 100 curated homologs for BWI76_RS16125 FitnessBrowser__Koxy:BWI76_RS16125 (255 a.a.)

Found high-coverage hits (≥70%) to 60 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    52% identity, 95% coverage of query (254 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    50% identity, 96% coverage of query (250 bits)

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    46% identity, 98% coverage of query (224 bits)

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    47% identity, 97% coverage of query (215 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    47% identity, 96% coverage of query (213 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    47% identity, 96% coverage of query (212 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    44% identity, 92% coverage of query (190 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    39% identity, 98% coverage of query (177 bits)

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    39% identity, 98% coverage of query (177 bits)

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    32% identity, 93% coverage of query (120 bits)

Build an alignment

Build an alignment for BWI76_RS16125 and 10 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

Q8KT44 carboxylesterase (EC 3.1.1.1) from Lacticaseibacillus casei (see paper)
    25% identity, 97% coverage of query (79.7 bits)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    27% identity, 90% coverage of query (77.4 bits)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    26% identity, 96% coverage of query (77.4 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    27% identity, 90% coverage of query (77.4 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    27% identity, 90% coverage of query (77.4 bits)

5cbkA / A0A0M5I297 Crystal structure of the strigolactone receptor shhtl5 from striga hermonthica (see paper)
    24% identity, 87% coverage of query (77.0 bits)

8dvcA Receptor shhtl5 from striga hermonthica in complex with strigolactone agonist gr24
    24% identity, 87% coverage of query (75.5 bits)

5z7xA / A0A0M4AMQ0 Crystal structure of striga hermonthica htl4 (shhtl4) (see paper)
    24% identity, 87% coverage of query (73.2 bits)

6eb3C Structural and enzymatic characterization of an esterase from a metagenomic library
    26% identity, 97% coverage of query (71.6 bits)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
    26% identity, 97% coverage of query (70.9 bits)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
    27% identity, 97% coverage of query (70.9 bits)

bpdF / AAB17100.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus sp. M5 (see paper)
    26% identity, 98% coverage of query (69.3 bits)

BIOH_SERMA / Q8GHL1 Pimeloyl-[acyl-carrier protein] methyl ester esterase; Biotin synthesis protein BioH; Carboxylesterase BioH; EC 3.1.1.85 from Serratia marcescens (see paper)
    27% identity, 91% coverage of query (67.4 bits)

TGND_ACIAD / Q6F9F4 (E)-2-((N-methylformamido)methylene)succinate hydrolase; MFMS hydrolase; EC 3.5.1.- from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
    24% identity, 92% coverage of query (67.0 bits)

5dnuA / A0A0M3PN85 Crystal structure of striga kai2-like protein in complex with karrikin (see paper)
    22% identity, 93% coverage of query (65.9 bits)

5z7yA Crystal structure of striga hermonthica htl7 (shhtl7)
    22% identity, 88% coverage of query (63.5 bits)

7snuA / A0A0M3PNA2 Crystal structure of shhtl7 from striga hermonthica in complex with strigolactone antagonist rg6 (see paper)
    22% identity, 88% coverage of query (63.2 bits)

RutD / b1009 putative aminoacrylate hydrolase RutD from Escherichia coli K-12 substr. MG1655 (see 2 papers)
RUTD_ECOLI / P75895 Putative carbamate hydrolase RutD; Aminohydrolase; EC 3.5.1.- from Escherichia coli (strain K12) (see 5 papers)
    26% identity, 96% coverage of query (63.2 bits)

7c8lA Hybrid designing of potent inhibitors of striga strigolactone receptor shhtl7
    22% identity, 88% coverage of query (63.2 bits)

7k38A Crystal structure of pisum sativum kai2 in complex with gr24-ent5ds product (see paper)
    21% identity, 92% coverage of query (62.0 bits)

5z89A Structural basis for specific inhibition of highly sensitive shhtl7 receptor
    21% identity, 88% coverage of query (61.6 bits)

2ocgA / Q86WA6 Crystal structure of human valacyclovir hydrolase (see paper)
    24% identity, 98% coverage of query (60.8 bits)

D14L_ORYSJ / Q10J20 Probable esterase D14L; Protein DWARF-14-like; Protein D14-like; Protein HEBIBA D14L from Oryza sativa subsp. japonica (Rice) (see paper)
    22% identity, 92% coverage of query (60.8 bits)

B0M0H6 carboxylesterase (EC 3.1.1.1) from Serratia sp. (see paper)
    28% identity, 91% coverage of query (60.8 bits)

2ociA Crystal structure of valacyclovir hydrolase complexed with a product analogue
    24% identity, 98% coverage of query (60.8 bits)

BPHL_HUMAN / Q86WA6 Valacyclovir hydrolase; VACVase; Valacyclovirase; Biphenyl hydrolase-like protein; Biphenyl hydrolase-related protein; Bph-rp; Breast epithelial mucin-associated antigen; MCNAA; EC 3.1.-.- from Homo sapiens (Human) (see 2 papers)
    24% identity, 98% coverage of query (60.5 bits)

BPOC_MYCTU / P9WNH1 Putative non-heme bromoperoxidase BpoC from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    26% identity, 99% coverage of query (58.5 bits)

5z7wB / A0A0M4AV81 Crystal structure of striga hermonthica htl1 (shhtl1) (see paper)
    20% identity, 96% coverage of query (53.9 bits)

6brtA / A0A0D9Z3K8 F-box protein cth with hydrolase (see paper)
    22% identity, 90% coverage of query (51.6 bits)

lnmJ / Q8GGP2 leinamycin polyketide synthase LnmJ from Streptomyces atroolivaceus (see 5 papers)
    22% identity, 93% coverage of query (51.6 bits)

4ihaA Crystal structure of rice dwarf14 (d14) in complex with a gr24 hydrolysis intermediate
    22% identity, 90% coverage of query (51.6 bits)

5zhsA Crystal structure of osd14 in complex with covalently bound kk052
    22% identity, 90% coverage of query (51.6 bits)

6ap8A Crystal structure of rice d14 bound to 2-(2-methyl-3-nitroanilino) benzoic acid
    22% identity, 90% coverage of query (51.6 bits)

5dj5A Crystal structure of rice dwarf14 in complex with synthetic strigolactone gr24
    22% identity, 90% coverage of query (51.6 bits)

5zhtA Crystal structure of osd14 in complex with covalently bound kk073
    22% identity, 90% coverage of query (51.6 bits)

5zhrA Crystal structure of osd14 in complex with covalently bound kk094
    22% identity, 90% coverage of query (51.6 bits)

5yz7A Crystal structure of osd14 in complex with d-ring-opened 7'-carba-4bd
    22% identity, 90% coverage of query (51.6 bits)

vppF / A0A075XAI2 N-formylmaleamate deformylase (EC 3.5.1.106) from Ochrobactrum sp. SJY1 (see paper)
    22% identity, 95% coverage of query (51.2 bits)

D14_ORYSJ / Q10QA5 Strigolactone esterase D14; Protein DWARF 14; Protein DWARF 88; Protein HIGH-TILLERING DWARF 2; EC 3.1.-.- from Oryza sativa subsp. japonica (Rice) (see 11 papers)
    22% identity, 90% coverage of query (51.2 bits)

bphD / BAM76235.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Janibacter sp. TYM3221 (see paper)
    27% identity, 97% coverage of query (50.8 bits)

4jymA / Q9SZU7 Crystal structure of kai2 in complex with 3-methyl-2h-furo[2,3- c]pyran-2-one (see paper)
    19% identity, 93% coverage of query (50.4 bits)

RSBQ_BACSU / O07015 Sigma factor SigB regulation protein RsbQ from Bacillus subtilis (strain 168) (see 2 papers)
    22% identity, 87% coverage of query (50.1 bits)

KAI2_ARATH / Q9SZU7 Probable esterase KAI2; Protein DWARF-14-like; Protein D14-like; Protein HYPOSENSITIVE TO LIGHT; Protein KARRIKIN INSENSITIVE 2 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
    19% identity, 93% coverage of query (50.1 bits)

7c4dA / A0A2S1GUX0 Marine microorganism esterase (see paper)
    21% identity, 96% coverage of query (49.7 bits)

BIOH_NEIMH / E6MWF8 Pimeloyl-[acyl-carrier protein] methyl ester esterase; Biotin synthesis protein BioH; Carboxylesterase BioH; EC 3.1.1.85 from Neisseria meningitidis serogroup B / serotype 15 (strain H44/76) (see paper)
    26% identity, 95% coverage of query (49.3 bits)

D14_ARATH / Q9SQR3 Strigolactone esterase D14; Protein DWARF 14; AtD14; EC 3.1.-.- from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
    19% identity, 90% coverage of query (48.9 bits)

RMS3_PEA / A0A109QYD3 Strigolactone esterase RMS3; Protein DWARF 14 homolog; PsD14; Protein RAMOSUS 3; EC 3.1.-.- from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see paper)
    20% identity, 94% coverage of query (48.5 bits)

1m33A / P13001 Crystal structure of bioh at 1.7 a (see paper)
    24% identity, 97% coverage of query (47.0 bits)

BioB / b3412 pimeloyl-acyl carrier protein methyl ester esterase (EC 3.1.1.85; EC 3.1.1.1) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
bioH / P13001 pimeloyl-acyl carrier protein methyl ester esterase (EC 3.1.1.85; EC 3.1.1.1) from Escherichia coli (strain K12) (see 8 papers)
BIOH_ECOLI / P13001 Pimeloyl-[acyl-carrier protein] methyl ester esterase; Biotin synthesis protein BioH; Carboxylesterase BioH; EC 3.1.1.85 from Escherichia coli (strain K12) (see 4 papers)
P13001 carboxylesterase (EC 3.1.1.1); pimelyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Escherichia coli (see paper)
    24% identity, 97% coverage of query (46.6 bits)

flnE / BAC75995.1 meta cleavage compound hydrolase from Terrabacter sp. DBF63 (see paper)
    24% identity, 93% coverage of query (45.1 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory