Searching for up to 100 curated homologs for BWI76_RS20540 BWI76_RS20540 NupC/NupG family nucleoside CNT transporter (400 a.a.)
Found high-coverage hits (≥70%) to 31 curated proteins.
You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.
Cru / b2393 nucleoside:H+ symporter NupC from Escherichia coli K-12 substr. MG1655 (see 6 papers)
nupC / P0AFF2 nucleoside:H+ symporter NupC from Escherichia coli (strain K12) (see 13 papers)
NUPC_ECOLI / P0AFF2 Nucleoside permease NupC; Nucleoside-transport system protein NupC from Escherichia coli (strain K12) (see 6 papers)
TC 2.A.41.1.1 / P0AFF2 Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017). ADP-glucose is also a substrate of this system from Escherichia coli (see 5 papers)
93% identity, 100% coverage of query (754 bits)
nupC / CAA52821.1 NupC from Escherichia coli (see paper)
93% identity, 100% coverage of query (743 bits)
NUPC_BACSU / P39141 Nucleoside permease NupC from Bacillus subtilis (strain 168) (see paper)
TC 2.A.41.1.2 / P39141 Pyrimidine-specific nucleoside:H+ symporter, NupC from Bacillus subtilis (see 4 papers)
58% identity, 98% coverage of query (434 bits)
NUPG_BACSU / P42312 Purine nucleoside transport protein NupG from Bacillus subtilis (strain 168) (see paper)
TC 2.A.41.1.3 / P42312 The purine nucleoside uptake transporter NupG (YxjA) from Bacillus subtilis (see 4 papers)
35% identity, 98% coverage of query (228 bits)
NupX / b2161 putative nucleoside transporter from Escherichia coli K-12 substr. MG1655 (see 2 papers)
nupX / P33021 putative nucleoside transporter from Escherichia coli (strain K12) (see paper)
NUPX_ECOLI / P33021 Putative nucleoside permease NupX from Escherichia coli (strain K12) (see paper)
TC 2.A.41.2.10 / P33021 Nucleoside permease NupX from Escherichia coli (strain K12) (see 4 papers)
31% identity, 99% coverage of query (172 bits)
5l26A Structure of cntnw in an inward-facing substrate-bound state
31% identity, 99% coverage of query (169 bits)
Build an alignment for BWI76_RS20540 and 6 homologs with ≥ 30% identity
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TC 2.A.41.2.11 / Q9KPL5 Concentrative nucleoside transporter, CNT, of 418 aas and 12 TMSs. A repeat-swapped model of VcCNT predicts that nucleoside transport occurs via a mechanism involving an elevator-like substrate binding domain movement across the membrane from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
28% identity, 98% coverage of query (167 bits)
PsuT / b2164 putative pseudouridine transporter from Escherichia coli K-12 substr. MG1655 (see 4 papers)
30% identity, 99% coverage of query (165 bits)
5l2aA / G4CRQ5 Structure of cntnw n149s,f366a in an outward-facing state (see paper)
30% identity, 99% coverage of query (165 bits)
4pb1A / Q9KPL5 Structure of vccnt-7c8c bound to ribavirin (see paper)
29% identity, 96% coverage of query (164 bits)
4pd5A Crystal structure of vccnt-7c8c bound to gemcitabine
29% identity, 96% coverage of query (164 bits)
4pd7A Structure of vccnt bound to zebularine
29% identity, 98% coverage of query (164 bits)
3tijA Crystal structure of a concentrative nucleoside transporter from vibrio cholerae
29% identity, 98% coverage of query (164 bits)
4pdaA Structure of vccnt-7c8c bound to cytidine
29% identity, 96% coverage of query (164 bits)
4pd9A Structure of vccnt-7c8c bound to adenosine
29% identity, 96% coverage of query (164 bits)
4pd8A Structure of vccnt-7c8c bound to pyrrolo-cytidine
29% identity, 96% coverage of query (164 bits)
4pb2A Structure of vccnt-7c8c bound to 5-fluorouridine
29% identity, 96% coverage of query (164 bits)
Shewana3_1039 nucleoside permease (cytidine, inosine, uridine, or thymidine) from Shewanella sp. ANA-3
28% identity, 100% coverage of query (163 bits)
nupC / O25792 purine nucleobase/nucleoside transporter from Helicobacter pylori (strain ATCC 700392 / 26695) (see 2 papers)
NUPC_HELPY / O25792 Nucleoside permease NupC from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
27% identity, 98% coverage of query (154 bits)
cntA nucleoside transporter (Eurofung) from Emericella nidulans (see paper)
25% identity, 100% coverage of query (126 bits)
TC 2.A.41.2.5 / Q9UA35 Broadly selective nucleoside:Na+ cotransporter, hfCNT (transports uridine, thymidine, inosine, 3'-azido-3'deoxythymidine, 2'3'dideoxycytidine, and 2'3'dideoxyinosine) (Na+/uridine = 2) from Eptatretus stoutii (Pacific hagfish) (see paper)
23% identity, 100% coverage of query (119 bits)
CNT cation-coupled nucleoside transporter (H(+)/nucleoside symporter) from Candida albicans (see 5 papers)
25% identity, 100% coverage of query (118 bits)
S28A1_HUMAN / O00337 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; hCNT1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Homo sapiens (Human) (see 9 papers)
TC 2.A.41.2.3 / O00337 Pyrimidine-preferring nucleoside:Na+ symporter, CNT1 (Na+/nucleoside = 2)(transports uridine, gemcitabine and 5'-deoxy-5'-fluorouridine) (Larráyoz et al., 2004), but in addition to pyrimidine nucelosides, it transports adenosine from Homo sapiens (Human) (see 5 papers)
26% identity, 97% coverage of query (117 bits)
S28A3_MOUSE / Q9ERH8 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; mCNT3 from Mus musculus (Mouse) (see paper)
TC 2.A.41.2.6 / Q9ERH8 Broad-specificity nucleoside:Na+, H+ and Li+ symporter, hCNT3 (Slc28a3) transports a broad range of both purine and pyrimidine nucleosides as well as anticancer and antiviral nucleoside drugs, but guanosine, 3'azido-3-deoxythymidine and 2',3'-dideoxycytidine, which are substrates with Na+, are not substrates with H+. Both of the two cation-binding sites can apparently bind Na+, but only one can bind H+, and the Na+ and H+ forms transport different ranges of substrates. (Note: Cnt1 and Cnt2 are Na+-specific.) from Homo sapiens (Human) (see 3 papers)
Slc28a3 / RF|NP_071712.3 solute carrier family 28 member 3 from Mus musculus (see paper)
25% identity, 84% coverage of query (115 bits)
S28A3_RAT / Q8VIH3 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; rCNT3 from Rattus norvegicus (Rat) (see paper)
25% identity, 84% coverage of query (113 bits)
S28A1_RAT / Q62674 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.41.2.2 / Q62674 Nonspecific nucleoside:Na+ symporter from Rattus norvegicus (Rat) (see paper)
Slc28a1 / RF|NP_446315.1 sodium/nucleoside cotransporter 1 from Rattus norvegicus
25% identity, 97% coverage of query (109 bits)
S28A1_PIG / O62667 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Sus scrofa (Pig) (see paper)
25% identity, 97% coverage of query (109 bits)
S28A3_HUMAN / Q9HAS3 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; hCNT3 from Homo sapiens (Human) (see 8 papers)
TC 2.A.41.2.8 / Q9HAS3 Solute carrier family 28 member 3 (Concentrative Na+-nucleoside cotransporter 3) (CNT 3) (hCNT3) from Homo sapiens (see 9 papers)
24% identity, 88% coverage of query (105 bits)
S28A2_HUMAN / O43868 Sodium/nucleoside cotransporter 2; Concentrative nucleoside transporter 2; CNT 2; hCNT2; Na(+)/nucleoside cotransporter 2; Sodium-coupled nucleoside transporter 2; Sodium/purine nucleoside co-transporter; SPNT; Solute carrier family 28 member 2 from Homo sapiens (Human) (see 4 papers)
TC 2.A.41.2.4 / O43868 Purine nucleoside, uridine, and 2'3'dideoxyinosine cladribrine:Na+ symporter, CNT2 (Na+/nucleoside = 1) from Homo sapiens (Human) (see 7 papers)
23% identity, 95% coverage of query (95.9 bits)
S28A1_RABIT / Q9MZT2 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Oryctolagus cuniculus (Rabbit) (see paper)
21% identity, 95% coverage of query (88.2 bits)
S28A2_RAT / Q62773 Sodium/nucleoside cotransporter 2; Concentrative nucleoside transporter 2; CNT 2; rCNT2; Na(+)/nucleoside cotransporter 2; Sodium-coupled nucleoside transporter 2; Sodium/purine nucleoside cotransporter; SPNT; Solute carrier family 28 member 2 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.41.2.1 / Q62773 Purine-specific nucleoside:Na+ symporter (see 3 papers)
21% identity, 95% coverage of query (87.4 bits)
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Lawrence Berkeley National Laboratory