Sites on a Tree

 

Searching for up to 100 curated homologs for BWI76_RS22825 FitnessBrowser__Koxy:BWI76_RS22825 (446 a.a.)

Found high-coverage hits (≥70%) to 25 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

YgcX / b2787 D-glucarate dehydratase (EC 4.2.1.40) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
gudD / P0AES2 D-glucarate dehydratase (EC 4.2.1.40) from Escherichia coli (strain K12) (see 5 papers)
GUDD_ECOLI / P0AES2 Glucarate dehydratase; GDH; GlucD; D-glucarate dehydratase; EC 4.2.1.40 from Escherichia coli (strain K12) (see 3 papers)
gudD / RF|NP_417267 glucarate dehydratase; EC 4.2.1.40 from Escherichia coli K12 (see 8 papers)
    97% identity, 100% coverage of query (911 bits)

1ec9D / P0AES2 E. Coli glucarate dehydratase bound to xylarohydroxamate (see paper)
    97% identity, 100% coverage of query (907 bits)

1ecqA E. Coli glucarate dehydratase bound to 4-deoxyglucarate
    97% identity, 100% coverage of query (907 bits)

1ec8A E. Coli glucarate dehydratase bound to product 2,3-dihydroxy-5-oxo- hexanedioate
    97% identity, 99% coverage of query (904 bits)

1jctA Glucarate dehydratase, n341l mutant orthorhombic form
    97% identity, 99% coverage of query (903 bits)

gudD / Q6FFQ2 D-glucarate dehydratase subunit (EC 4.2.1.40) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
GUDD_ACIAD / Q6FFQ2 Glucarate dehydratase; GDH; GlucD; EC 4.2.1.40 from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
Q6FFQ2 glucarate dehydratase (EC 4.2.1.40) from Acinetobacter baylyi (see paper)
    71% identity, 99% coverage of query (697 bits)

gudD / P42206 D-glucarate dehydratase subunit (EC 4.2.1.40) from Pseudomonas putida (see paper)
GUDD_PSEPU / P42206 Glucarate dehydratase; GDH; GlucD; EC 4.2.1.40 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
    71% identity, 100% coverage of query (657 bits)

3p0wB / B2UIZ1 Crystal structure of d-glucarate dehydratase from ralstonia solanacearum complexed with mg and d-glucarate
    67% identity, 98% coverage of query (581 bits)

GudX / b2788 glucarate dehydratase-related protein from Escherichia coli K-12 substr. MG1655 (see 2 papers)
GUDX_ECOLI / Q46915 Glucarate dehydratase-related protein; GDH-RP; GlucDRP; EC 4.2.1.- from Escherichia coli (strain K12) (see paper)
gudX / RF|NP_417268 glucarate dehydratase-related protein from Escherichia coli K12
    63% identity, 99% coverage of query (566 bits)

3nxlC / Q39KL8 Crystal structure of glucarate dehydratase from burkholderia cepacia complexed with magnesium
    64% identity, 98% coverage of query (550 bits)

3nfuA / Q1QUN0 Crystal structure of probable glucarate dehydratase from chromohalobacter salexigens dsm 3043 complexed with magnesium
    59% identity, 98% coverage of query (542 bits)

3n6hB / A6VQF6 Crystal structure of mandelate racemase/muconate lactonizing protein from actinobacillus succinogenes 130z complexed with magnesium/sulfate
    60% identity, 99% coverage of query (542 bits)

3pfrA Crystal structure of d-glucarate dehydratase related protein from actinobacillus succinogenes complexed with d-glucarate
    60% identity, 99% coverage of query (540 bits)

4it1D / Q3KB33 Crystal structure of enolase pfl01_3283 (target efi-502286) from pseudomonas fluorescens pf0-1 with bound magnesium, potassium and tartrate
    36% identity, 97% coverage of query (228 bits)

3va8A Crystal structure of enolase fg03645.1 (target efi-502278) from gibberella zeae ph-1 complexed with magnesium, formate and sulfate
    34% identity, 97% coverage of query (225 bits)

3vc6A / D6Y7Y6 Crystal structure of enolase tbis_1083(target efi-502310) from thermobispora bispora dsm 43833 complexed with magnesium and formate
    34% identity, 96% coverage of query (220 bits)

Build an alignment

Build an alignment for BWI76_RS22825 and 16 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

D4GYD4 gluconate dehydratase (EC 4.2.1.39) from Haloferax volcanii (see paper)
    23% identity, 83% coverage of query (66.2 bits)

gci / A9CEQ8 D-galactarolactone cycloisomerase monomer (EC 5.5.1.27) from Agrobacterium fabrum (strain C58 / ATCC 33970) (see paper)
GCI_AGRFC / A9CEQ8 D-galactarolactone cycloisomerase; EC 5.5.1.27 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see 2 papers)
A9CEQ8 D-galactarolactone cycloisomerase (EC 5.5.1.27) from Agrobacterium tumefaciens (see paper)
4hpnA / A9CEQ8 Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
    24% identity, 83% coverage of query (63.2 bits)

catB / BAE46388.1 cis,cis-muconate cycloisomerase from Rhodococcus sp. AN-22 (see paper)
    24% identity, 83% coverage of query (62.8 bits)

2gghC / Q9RYA6 The mutant a68c-d72c-nlq of deinococcus radiodurans nacylamino acid racemase (see paper)
    24% identity, 74% coverage of query (55.8 bits)

3i6eA / Q5LM96 Crystal structure of muconate lactonizing enzyme from ruegeria pomeroyi.
    22% identity, 76% coverage of query (48.1 bits)

3cb3A / Q12GE3 Crystal structure of l-talarate dehydratase from polaromonas sp. Js666 complexed with mg and l-glucarate
    22% identity, 89% coverage of query (47.0 bits)

3ozmA / A0A0H3LT39 Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
    23% identity, 93% coverage of query (46.6 bits)

3ozmD Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
    23% identity, 90% coverage of query (45.8 bits)

2og9A Crystal structure of mandelate racemase/muconate lactonizing enzyme from polaromonas sp. Js666
    22% identity, 81% coverage of query (45.4 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory