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Searching for up to 100 curated homologs for BWI76_RS25275 FitnessBrowser__Koxy:BWI76_RS25275 (162 a.a.)

Found high-coverage hits (≥70%) to 20 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

PtsN / b3204 phosphotransferase system enzyme IIA(Ntr) from Escherichia coli K-12 substr. MG1655 (see 22 papers)
ptsN / AAB60165.1 Enzyme IIANtr from Escherichia coli (see 3 papers)
    90% identity, 100% coverage of query (298 bits)

TC 4.A.2.1.14 / D2RXA7 Putative PTS IIA-like nitrogen-regulatory protein PtsN, component of Fructose Enzyme II complex (IIAFru - IIBFru - IICFru) (based on homology) from Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / NCIMB 13204 / VKM B-1734) (see paper)
    36% identity, 91% coverage of query (77.8 bits)

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Build an alignment for BWI76_RS25275 and 2 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

TC 4.A.2.1.20 / Q3K0G6 Chromosomal fructose Enzyme IIABC (Fru1) of 654 aas; in an operon with fructose-1-P kinase from Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
    29% identity, 88% coverage of query (70.9 bits)

TC 4.A.2.1.4 / P71012 The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system from Bacillus subtilis (see paper)
    26% identity, 95% coverage of query (68.6 bits)

PTFA_HALVD / D4GYE4 PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
TC 4.A.2.1.15 / D4GYE4 Sugar phosphotransferase system IIA component, component of Fructose-specific PTS, PtfABC from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (see paper)
    32% identity, 80% coverage of query (68.2 bits)

PTN3B_BACSU / O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
O31645 protein-Npi-phosphohistidine-D-mannose phosphotransferase (EC 2.7.1.191) from Bacillus subtilis (see paper)
TC 4.A.2.1.6 / O31645 The mannose porter (ManP) (37% identical to 4.A.2.1.1). It is encoded in an operon with 3 genes: from Bacillus subtilis (see 5 papers)
    28% identity, 91% coverage of query (67.8 bits)

MngA 2-O-α-mannosyl-D-glycerate specific PTS enzyme II (EC 2.7.1.195) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
MngA / P54745 2-O-α-mannosyl-D-glycerate specific PTS enzyme II (EC 2.7.1.195) from Escherichia coli (strain K12) (see 5 papers)
MNGA_ECOLI / P54745 PTS system 2-O-alpha-mannosyl-D-glycerate-specific EIIABC component; 2-O-alpha-mannosyl-D-glycerate-specific phosphotransferase enzyme MngA; Protein-Npi-phosphohistidine--2-O-alpha-mannosyl-D-glycerate phosphotransferase; EC 2.7.1.195 from Escherichia coli (strain K12) (see 2 papers)
P54745 protein-Npi-phosphohistidine-2-O-alpha-mannosyl-D-glycerate phosphotransferase (EC 2.7.1.195) from Escherichia coli (see paper)
TC 4.A.2.1.3 / P54745 The 2-O-α-mannosyl D-glycerate porter (2-O-α-mannosyl D-glycerate-6-P forming), MngA (HrsA) from Escherichia coli (see 5 papers)
    31% identity, 81% coverage of query (66.2 bits)

TC 4.A.2.1.7 / Q1LZ59 The fructose inducible fructose/xylitol porter, FruI from Streptococcus mutans UA159 (see paper)
    26% identity, 88% coverage of query (65.5 bits)

Q8DWE6 protein-Npi-phosphohistidine-D-fructose phosphotransferase (subunit 1/2) (EC 2.7.1.202) from Streptococcus mutans serotype c (see paper)
TC 4.A.2.1.8 / Q1LZ68 Constitutive fructose permease aka FruD, component of The constitutive fructose porter FruC/FruD from Streptococcus mutans UA159 (see paper)
    26% identity, 88% coverage of query (64.3 bits)

tagM / A0A1Y0YTF1 tagatose-specific PTS enzyme IIA component from Bacillus licheniformis (see 2 papers)
TC 4.A.2.1.23 / T5HNP5 PTS fructose transporter subunit IIA, component of The tagatose-1-P-forming tagatose phosphorylating Enzyme IIA/IIBC, TagM/L (Van der Heiden et al. 2015). The product is phosphorylated by tagatose-1-P kinase (TagK), and then cleaved by tagatose-1,6-bisphosphate aldolase (GatY) from Bacillus licheniformis CG-B52
    32% identity, 85% coverage of query (61.2 bits)

TC 4.A.2.1.22 / Q3JZE3 PTS system, fructose-specific, IIA component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence from Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
    27% identity, 89% coverage of query (58.2 bits)

TC 4.A.2.1.9 / Q1R4S9 Putative phosphotransferase system (PTS), fructose-specific, component of The FrzABC PTS putative transporter (promotes bacterial fitness under stress conditions and promotes fimbrial (fim) gene expression indirectly (Rouquet et al., 2009). Might transport D-tagatose, D-psicose and/or D-sorbose, or a disaccharide of these (Rouquet et al. 2009); involved in environmental sensing, host adaptation and virulence from Escherichia coli (strain UTI89 / UPEC) (see paper)
    28% identity, 94% coverage of query (55.1 bits)

PS417_03965 fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components from Pseudomonas simiae WCS417
    34% identity, 80% coverage of query (52.8 bits)

FrvA / b3900 putative PTS enzyme IIA component FrvA from Escherichia coli K-12 substr. MG1655 (see paper)
TC 4.A.2.1.25 / P32155 Fructose-like phosphotransferase enzyme IIA component, component of Fructose-like PTS Enzyme II complex, FrvA (IIA of 148 aas) - FrvB (IIBC of 483 aas and 9 predicted TMSs) from Escherichia coli (strain K12)
    28% identity, 73% coverage of query (49.7 bits)

FruB / b2169 fructose-specific PTS multiphosphoryl transfer protein FruB from Escherichia coli K-12 substr. MG1655 (see 4 papers)
FruB / P69811 fructose-specific PTS multiphosphoryl transfer protein FruB from Escherichia coli (strain K12) (see 2 papers)
PTFAH_ECOLI / P69811 Multiphosphoryl transfer protein; MTP; Diphosphoryl transfer protein; DTP; Phosphotransferase FPr protein; Pseudo-HPr from Escherichia coli (strain K12) (see paper)
TC 4.A.2.1.1 / P69811 PTFA aka FRUB aka FRUF aka FPR aka B2169, component of Fructose porter (FruAB) (fructose-1-P forming) from Escherichia coli (see 5 papers)
    31% identity, 75% coverage of query (47.8 bits)

PTFAH_VIBCH / Q9KM70 Multiphosphoryl transfer protein; MTP from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see 2 papers)
    28% identity, 81% coverage of query (46.6 bits)

PTFAH_SALTY / P17127 Multiphosphoryl transfer protein; MTP; Diphosphoryl transfer protein; DTP; Phosphotransferase FPr protein; Pseudo-HPr from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
    30% identity, 75% coverage of query (46.2 bits)

SgaR2 / VIMSS5937003 SgaR2 regulator of Ascorbate utilization, effector SgaB2, ascorbate-specific PTS system, EIIB component; HPr, phosphocarrier protein from Streptococcus uberis 0140J
    29% identity, 88% coverage of query (45.4 bits)

1j6tA / P00550 Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
    26% identity, 78% coverage of query (43.1 bits)

C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168)
    27% identity, 88% coverage of query (42.0 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory