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Searching for up to 100 curated homologs for CCNA_01001 FitnessBrowser__Caulo:CCNA_01001 (303 a.a.)

Found high-coverage hits (≥70%) to 100 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

8cqxA / A0A0B0SD75 Ribokinase from t.Sp mutant a92g
    45% identity, 99% coverage of query (204 bits)

SMc01103 Ribokinase (EC 2.7.1.15) from Sinorhizobium meliloti 1021
    41% identity, 99% coverage of query (187 bits)

BT2804 ribokinase (EC 2.7.1.15) from Bacteroides thetaiotaomicron VPI-5482
    37% identity, 99% coverage of query (168 bits)

AO356_00950 Ribokinase (EC 2.7.1.15) from Pseudomonas fluorescens FW300-N2C3
    40% identity, 98% coverage of query (163 bits)

BWI76_RS00290 Ribokinase (EC 2.7.1.15) from Klebsiella michiganensis M5al
    39% identity, 99% coverage of query (162 bits)

AO353_20835 Ribokinase (EC 2.7.1.15) from Pseudomonas fluorescens FW300-N2E3
    39% identity, 98% coverage of query (162 bits)

6znxC / A0A0B0SD75 Ribokinase from thermus species
    40% identity, 99% coverage of query (160 bits)

A0A0H2UL04 ribokinase (EC 2.7.1.15) from Vibrio cholerae (see paper)
    40% identity, 98% coverage of query (156 bits)

4xckA / A0A0H2UL04 Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
    40% identity, 98% coverage of query (156 bits)

RbsK / b3752 ribokinase (EC 2.7.1.15) from Escherichia coli K-12 substr. MG1655 (see 20 papers)
rbsK / P0A9J6 ribokinase (EC 2.7.1.15) from Escherichia coli (strain K12) (see 19 papers)
RBSK_ECOLI / P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 5 papers)
rbsK / RF|NP_418208 ribokinase from Escherichia coli K12 (see 4 papers)
    37% identity, 99% coverage of query (156 bits)

1gqtB / P0A9J6 Activation of ribokinase by monovalent cations (see paper)
    37% identity, 99% coverage of query (156 bits)

1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121
    37% identity, 99% coverage of query (156 bits)

5c41A / Q9H477 Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
    36% identity, 99% coverage of query (155 bits)

6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
    36% identity, 99% coverage of query (155 bits)

6wjzA Crystal structure of human ribokinase in complex with ampcp
    36% identity, 99% coverage of query (155 bits)

RBSK_SCHPO / O60116 Ribokinase; RK; EC 2.7.1.15 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    36% identity, 98% coverage of query (154 bits)

Pf1N1B4_6031 Ribokinase (EC 2.7.1.15) from Pseudomonas fluorescens FW300-N1B4
    39% identity, 98% coverage of query (154 bits)

5c3yA Structure of human ribokinase crystallized with amppnp
    36% identity, 97% coverage of query (154 bits)

2fv7A Crystal structure of human ribokinase
    36% identity, 97% coverage of query (154 bits)

5byfA Crystal structure of human ribokinase in complex with amp
    36% identity, 98% coverage of query (152 bits)

RBSK_HUMAN / Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human) (see paper)
Q9H477 ribokinase (EC 2.7.1.15) from Homo sapiens (see paper)
    36% identity, 96% coverage of query (151 bits)

RBSK_LEIMA / E9AD19 Ribokinase; RK; EC 2.7.1.15 from Leishmania major (see paper)
    35% identity, 98% coverage of query (149 bits)

6a8aA / A0A3S7X0F5 Ribokinase from leishmania donovani with atp (see paper)
    35% identity, 98% coverage of query (149 bits)

RBSK_STAAC / A0A0H2WZY4 Ribokinase; RK; EC 2.7.1.15 from Staphylococcus aureus (strain COL) (see paper)
A0A0H2WZY4 ribokinase (EC 2.7.1.15) from Staphylococcus aureus (see paper)
    35% identity, 97% coverage of query (149 bits)

RBSKI_FRUSL / P83534 Bifunctional ribokinase/ribose-5-phosphate isomerase A; EC 2.7.1.15; EC 5.3.1.6 from Fructilactobacillus sanfranciscensis (strain ATCC 27651 / DSM 20451 / JCM 5668 / CCUG 30143 / KCTC 3205 / NCIMB 702811 / NRRL B-3934 / L-12) (Lactobacillus sanfranciscensis) (see paper)
    35% identity, 97% coverage of query (149 bits)

6a8cA Ribokinase from leishmania donovani with adp
    35% identity, 98% coverage of query (149 bits)

6a8bA Ribokinase from leishmania donovani with amppcp
    35% identity, 98% coverage of query (149 bits)

HSERO_RS11500 Ribokinase (EC 2.7.1.15) from Herbaspirillum seropedicae SmR1
    37% identity, 97% coverage of query (148 bits)

BT2803 ribokinase (EC 2.7.1.15) from Bacteroides thetaiotaomicron VPI-5482
    33% identity, 97% coverage of query (141 bits)

deoK / Q8XGW5 deoxyribokinase monomer (EC 2.7.1.229) from Salmonella typhi (see 4 papers)
DEOK_SALTI / P0DX97 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhi (see paper)
DEOK_SALTY / Q7CPF5 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
    31% identity, 99% coverage of query (140 bits)

Q8NQR0 ribokinase (EC 2.7.1.15) from Corynebacterium glutamicum (see paper)
    36% identity, 96% coverage of query (139 bits)

BPHYT_RS25805 deoxyribose kinase (EC 2.7.1.15) from Burkholderia phytofirmans PsJN
    35% identity, 99% coverage of query (137 bits)

6ilsB / A1A6H3 Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
    32% identity, 99% coverage of query (126 bits)

RBSK / A1A6H3 ribokinase (EC 2.7.1.15) from Arabidopsis thaliana (see 2 papers)
RBSK_ARATH / A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
A1A6H3 ribokinase (EC 2.7.1.15) from Arabidopsis thaliana (see paper)
    32% identity, 98% coverage of query (125 bits)

RBSK_YEAST / P25332 Ribokinase; RK; EC 2.7.1.15 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
    31% identity, 98% coverage of query (125 bits)

H281DRAFT_01116 deoxyribose kinase (EC 2.7.1.15) from Paraburkholderia bryophila 376MFSha3.1
    37% identity, 99% coverage of query (121 bits)

P71913 ribokinase (EC 2.7.1.15) from Mycobacterium tuberculosis (see paper)
    39% identity, 85% coverage of query (112 bits)

3go6A / P71913 Crystal structure of m. Tuberculosis ribokinase (rv2436) in complex with ribose and amp-pnp
    39% identity, 85% coverage of query (112 bits)

3go6B Crystal structure of m. Tuberculosis ribokinase (rv2436) in complex with ribose and amp-pnp
    39% identity, 85% coverage of query (112 bits)

6xk2A / J9VQ51 Crystal structure of ribokinase from cryptococcus neoformans var. Grubii serotype a in complex with adp
    33% identity, 99% coverage of query (107 bits)

Build an alignment

Build an alignment for CCNA_01001 and 40 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

3ikhA / A6T989 Crystal structure of ribokinase in complex with atp and glycerol in the active site from klebsiella pneumoniae
    28% identity, 99% coverage of query (105 bits)

3i3yD Crystal structure of ribokinase in complex with d-ribose from klebsiella pneumoniae
    28% identity, 99% coverage of query (105 bits)

KDGK_THETE / Q704D0 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermoproteus tenax (see paper)
kdgK / CAF18464.1 2-Keto-3-deoxy-gluconate kinase from Thermoproteus tenax (see paper)
    30% identity, 97% coverage of query (79.7 bits)

SCRK1_ORYSJ / Q0JGZ6 Fructokinase-1; Fructokinase I; OsFKI; EC 2.7.1.4 from Oryza sativa subsp. japonica (Rice) (see paper)
    32% identity, 91% coverage of query (79.3 bits)

SCRK1_MAIZE / Q6XZ79 Fructokinase-1; ZmFRK1; EC 2.7.1.4 from Zea mays (Maize)
    31% identity, 91% coverage of query (79.3 bits)

TK2029 / Q5JDG9 α-D-ribose-1-phosphate 5-kinase (ADP) (EC 2.7.1.212) from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (see paper)
R1PKD_THEKO / Q5JDG9 ADP-dependent ribose-1-phosphate kinase; ADP-R1P kinase; ADP:alpha-D-ribose-1-phosphate 5-phosphotransferase; Alpha-D-ribose-1-phosphate 5-kinase (ADP); EC 2.7.1.212 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
    29% identity, 98% coverage of query (79.3 bits)

SCRK1_ORYSI / A2WXV8 Fructokinase-1; Fructokinase I; OsFKI; EC 2.7.1.4 from Oryza sativa subsp. indica (Rice) (see paper)
    32% identity, 91% coverage of query (78.2 bits)

7ag6A / P32143 Crystal structure of sf kinase yihv from e. Coli in complex with sulfofructose (sf), adp-mg (see paper)
    27% identity, 96% coverage of query (74.7 bits)

SquV / b3883 6-deoxy-6-sulfofructose kinase (EC 2.7.1.184) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
yihV / P32143 6-deoxy-6-sulfofructose kinase (EC 2.7.1.184) from Escherichia coli (strain K12) (see 2 papers)
SQUV_ECOLI / P32143 Sulfofructose kinase; SF kinase; EC 2.7.1.184 from Escherichia coli (strain K12) (see 2 papers)
    27% identity, 96% coverage of query (74.7 bits)

7agkA Crystal structure of e. Coli sf kinase (yihv) in complex with product sulfofructose phosphate (sfp)
    27% identity, 96% coverage of query (74.7 bits)

7aghA Crystal structure of sf kinase yihv from e. Coli in complex with amppnp-mg
    28% identity, 99% coverage of query (74.7 bits)

Q9C524 fructokinase (EC 2.7.1.4) from Arabidopsis thaliana (see 2 papers)
    28% identity, 88% coverage of query (72.8 bits)

KDGK_SULTO / Q96XN9 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxy-D-gluconate kinase; KDG kinase; KDGK; EC 2.7.1.45 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
Q96XN9 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) from Sulfurisphaera tokodaii (see paper)
    24% identity, 89% coverage of query (70.5 bits)

2dcnA / Q96XN9 Crystal structure of 2-keto-3-deoxygluconate kinase from sulfolobus tokodaii complexed with 2-keto-6-phosphogluconate (alpha-furanose form)
    24% identity, 89% coverage of query (70.5 bits)

iolC / P42414 5-dehydro-2-deoxy-D-gluconate kinase (EC 2.7.1.92) from Bacillus subtilis (strain 168) (see 2 papers)
IOLC_BACSU / P42414 5-dehydro-2-deoxygluconokinase; 2-deoxy-5-keto-D-gluconate kinase; DKG kinase; EC 2.7.1.92 from Bacillus subtilis (strain 168) (see paper)
    23% identity, 97% coverage of query (68.6 bits)

3iq0B / A0A0H2VDX9 Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
    25% identity, 84% coverage of query (67.4 bits)

1tz3A / Q8ZKR2 Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
    30% identity, 87% coverage of query (67.4 bits)

1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog
    30% identity, 87% coverage of query (67.4 bits)

Q9LNE4 fructokinase (EC 2.7.1.4) from Arabidopsis thaliana (see paper)
    29% identity, 91% coverage of query (65.9 bits)

Q9M1B9 fructokinase (EC 2.7.1.4) from Arabidopsis thaliana (see paper)
    27% identity, 88% coverage of query (65.9 bits)

Q42896 fructokinase (EC 2.7.1.4) from Solanum lycopersicum (see paper)
    28% identity, 92% coverage of query (65.9 bits)

P37829 fructokinase (EC 2.7.1.4) from Solanum tuberosum (see 2 papers)
    28% identity, 92% coverage of query (65.9 bits)

YhfQ / b3374 fructoselysine 6-kinase (EC 2.7.1.218) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
frlD / P45543 fructoselysine 6-kinase (EC 2.7.1.218) from Escherichia coli (strain K12) (see 3 papers)
FRLD_ECOLI / P45543 Fructoselysine 6-kinase; EC 2.7.1.218 from Escherichia coli (strain K12) (see 2 papers)
P45543 fructoselysine 6-kinase (EC 2.7.1.218) from Escherichia coli (see 3 papers)
    28% identity, 83% coverage of query (65.5 bits)

ADO1 putative uncharacterized protein from Candida albicans (see paper)
    27% identity, 78% coverage of query (64.3 bits)

ADK_PHYPA / O49923 Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20 from Physcomitrium patens (Spreading-leaved earth moss) (Physcomitrella patens) (see paper)
    26% identity, 94% coverage of query (63.9 bits)

O04897 fructokinase (EC 2.7.1.4) from Solanum lycopersicum (see paper)
    28% identity, 94% coverage of query (63.9 bits)

ADK_CRIGR / P55262 Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20 from Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (see paper)
    26% identity, 89% coverage of query (63.5 bits)

STM4066 / Q8ZKR2 aminoimidazole riboside kinase (EC 2.7.1.223) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
AIRSK_SALTY / Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
Q8ZKR2 aminoimidazole riboside kinase (EC 2.7.1.223) from Salmonella enterica subsp. enterica serovar Typhimurium (see 2 papers)
    29% identity, 87% coverage of query (63.5 bits)

Q9FLH8 fructokinase (EC 2.7.1.4) from Arabidopsis thaliana (see 2 papers)
    25% identity, 87% coverage of query (63.5 bits)

Q42645 fructokinase (EC 2.7.1.4) from Beta vulgaris (see paper)
    26% identity, 84% coverage of query (63.2 bits)

Q9SID0 fructokinase (EC 2.7.1.4) from Arabidopsis thaliana (see paper)
    27% identity, 88% coverage of query (62.8 bits)

KDGK_THET8 / Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
    28% identity, 92% coverage of query (62.4 bits)

1v1aA / Q53W83 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
    27% identity, 92% coverage of query (61.6 bits)

2i6aA / P55263 Human adenosine kinase in complex with 5'-deoxy-5-iodotubercidin (see paper)
    25% identity, 89% coverage of query (61.2 bits)

ADK / P55263 adenosine kinase (EC 2.7.1.20) from Homo sapiens (see 2 papers)
ADK_HUMAN / P55263 Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20 from Homo sapiens (Human) (see 4 papers)
    25% identity, 89% coverage of query (61.2 bits)

1bx4A Structure of human adenosine kinase at 1.50 angstroms
    25% identity, 89% coverage of query (61.2 bits)

HSERO_RS12125 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) from Herbaspirillum seropedicae SmR1
    22% identity, 90% coverage of query (60.8 bits)

Q9YG89 6-phosphofructokinase (EC 2.7.1.11) from Aeropyrum pernix (see 2 papers)
    27% identity, 84% coverage of query (60.5 bits)

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp
    27% identity, 91% coverage of query (60.5 bits)

5kb5A Crystal structure of the adenosine kinase from mus musculus in complex with adenosine and adenosine-diphosphate
    24% identity, 89% coverage of query (60.1 bits)

ADK_MOUSE / P55264 Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20 from Mus musculus (Mouse) (see paper)
P55264 adenosine kinase (EC 2.7.1.20) from Mus musculus (see 3 papers)
    24% identity, 89% coverage of query (59.7 bits)

Q5DKU6 adenosine kinase (EC 2.7.1.20) from Nicotiana tabacum (see paper)
    26% identity, 89% coverage of query (59.3 bits)

Pf6N2E2_512 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) from Pseudomonas fluorescens FW300-N2E2
    23% identity, 100% coverage of query (58.5 bits)

ADK1 / Q9SF85 adenosine kinase 1 (EC 2.7.1.20) from Arabidopsis thaliana (see paper)
ADK1_ARATH / Q9SF85 Adenosine kinase 1; AK 1; Adenosine 5'-phosphotransferase 1; EC 2.7.1.20 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9SF85 adenosine kinase (EC 2.7.1.20) from Arabidopsis thaliana (see paper)
    24% identity, 94% coverage of query (58.5 bits)

Q5DKU8 adenosine kinase (EC 2.7.1.20) from Nicotiana tabacum (see paper)
    25% identity, 90% coverage of query (57.8 bits)

Q5DKU9 adenosine kinase (EC 2.7.1.20) from Nicotiana tabacum (see paper)
    25% identity, 92% coverage of query (57.8 bits)

3in1A / P77493 Crystal structure of a putative ribokinase in complex with adp from e.Coli
    27% identity, 88% coverage of query (57.4 bits)

YdjH / b1772 L-glycero-L-galacto-octuluronate kinase from Escherichia coli K-12 substr. MG1655 (see 2 papers)
ydjH / P77493 L-glycero-L-galacto-octuluronate kinase from Escherichia coli (strain K12) (see 2 papers)
    27% identity, 88% coverage of query (57.4 bits)

Sama_0561 Fructokinase (EC 2.7.1.4) from Shewanella amazonensis SB2B
    28% identity, 88% coverage of query (57.4 bits)

F8D4I6 α-D-ribose-1-phosphate 5-kinase (ATP) (EC 2.7.1.239) from Halopiger xanaduensis (strain DSM 18323 / JCM 14033 / SH-6) (see 2 papers)
R1PKT_HALXS / F8D4I6 ATP-dependent ribose-1-phosphate kinase; ATP-R1P kinase; Alpha-D-ribose-1-phosphate 5-kinase (ATP); Hx-RbsK; EC 2.7.1.239 from Halopiger xanaduensis (strain DSM 18323 / JCM 14033 / SH-6) (see paper)
    29% identity, 99% coverage of query (57.0 bits)

AO356_23160 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) from Pseudomonas fluorescens FW300-N2C3
    22% identity, 100% coverage of query (57.0 bits)

O82616 fructokinase (EC 2.7.1.4) from Arabidopsis thaliana (see paper)
    26% identity, 88% coverage of query (56.2 bits)

4o1lA Human adenosine kinase in complex with inhibitor
    25% identity, 85% coverage of query (56.2 bits)

2i6bA Human adenosine kinase in complex with an acetylinic inhibitor
    25% identity, 84% coverage of query (55.8 bits)

HldE / b3052 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (EC 2.7.1.167; EC 2.7.7.70) from Escherichia coli K-12 substr. MG1655 (see 16 papers)
hldE / P76658 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (EC 2.7.1.167; EC 2.7.7.70) from Escherichia coli (strain K12) (see 15 papers)
HLDE_ECOLI / P76658 Bifunctional protein HldE; EC 2.7.1.167; EC 2.7.7.70 from Escherichia coli (strain K12) (see 2 papers)
P76658 D-glycero-beta-D-manno-heptose-7-phosphate kinase (EC 2.7.1.167); D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase (EC 2.7.7.70) from Escherichia coli (see 3 papers)
hldE / GB|AAC76088.1 D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenosyltransferase; EC 2.7.1.-; EC 2.7.7.- from Escherichia coli K12 (see 6 papers)
    30% identity, 84% coverage of query (52.8 bits)

3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
    25% identity, 88% coverage of query (51.2 bits)

3ih0A / O59128 Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
    25% identity, 88% coverage of query (51.2 bits)

3b1pA Structure of burkholderia thailandensis nucleoside kinase (bthnk) in complex with adp-inosine
    24% identity, 88% coverage of query (50.4 bits)

3b1rA Structure of burkholderia thailandensis nucleoside kinase (bthnk) in complex with amp-mg-amp
    24% identity, 88% coverage of query (50.4 bits)

3b1nB / Q2SZE4 Structure of burkholderia thailandensis nucleoside kinase (bthnk) in complex with adp-mizoribine (see paper)
    24% identity, 88% coverage of query (50.4 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory