Sites on a Tree

 

Searching for up to 100 curated homologs for Dsui_3297 FitnessBrowser__PS:Dsui_3297 (217 a.a.)

Found high-coverage hits (≥70%) to 49 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

6czpA / Q7MCD2 2.2 angstrom resolution crystal structure oxygen-insensitive NAD(p)h- dependent nitroreductase nfsb from vibrio vulnificus in complex with fmn
    53% identity, 100% coverage of query (246 bits)

7uwtB Structure of oxygen-insensitive NAD(p)h-dependent nitroreductase nfsb_vv f70a/f108y (ntr 2.0) in complex with fmn at 1.85 angstroms resolution
    52% identity, 100% coverage of query (241 bits)

P15888 nitrobenzene nitroreductase (EC 1.7.1.16) from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
    50% identity, 100% coverage of query (240 bits)

8dorA / A6T5Y2 Crystal structure of dihydropteridine reductase/oxygen-insensitive NAD(p)h nitroreductase from klebsiella pneumoniae
    50% identity, 100% coverage of query (238 bits)

NFSB_ENTCL / Q01234 Oxygen-insensitive NAD(P)H nitroreductase; NR; EC 1.-.-.- from Enterobacter cloacae (see 2 papers)
Q01234 nitrobenzene nitroreductase (EC 1.7.1.16) from Enterobacter cloacae (see paper)
    49% identity, 100% coverage of query (236 bits)

5j8dA / Q01234 Structure of nitroreductase from e. Cloacae complexed with nicotinic acid adenine dinucleotide (see paper)
    49% identity, 99% coverage of query (234 bits)

5j8gA Structure of nitroreductase from e. Cloacae complexed with para- nitrobenzoic acid
    49% identity, 99% coverage of query (234 bits)

1kqcA Structure of nitroreductase from e. Cloacae complex with inhibitor acetate
    49% identity, 99% coverage of query (234 bits)

Ntr / b0578 NAD(P)H nitroreductase NfsB (EC 1.5.1.34) from Escherichia coli K-12 substr. MG1655 (see 32 papers)
nfsB / P38489 NAD(P)H-dependent nitroreductase NfsB (EC 1.6.5.2; EC 1.5.1.34) from Escherichia coli (strain K12) (see 46 papers)
NFSB_ECOLI / P38489 Oxygen-insensitive NAD(P)H nitroreductase; Dihydropteridine reductase; FMN-dependent nitroreductase; EC 1.-.-.-; EC 1.5.1.34 from Escherichia coli (strain K12) (see 4 papers)
1ds7A / P38489 A minor fmn-dependent nitroreductase from escherichia coli b (see paper)
nfnB / PDB|1OO5_A oxygen-insensitive NAD(P)H nitroreductase; EC 1.-.-.-; EC 1.5.1.34 from Escherichia coli K12 (see 12 papers)
    48% identity, 100% coverage of query (232 bits)

1ooqA Nitroreductase from e-coli in complex with the inhibitor dicoumarol
    48% identity, 100% coverage of query (232 bits)

1oo6A Nitroreductase from e-coli in complex with the dinitrobenzamide prodrug sn23862
    48% identity, 100% coverage of query (232 bits)

1idtA Structural studies on a prodrug-activating system-cb1954 and fmn- dependent nitroreductase
    48% identity, 100% coverage of query (232 bits)

7xwwA Crystal structure of ntr in complex with bn-xb
    48% identity, 100% coverage of query (230 bits)

7x32A Crystal structure of e. Coli nfsb in complex with berberine
    48% identity, 100% coverage of query (230 bits)

1ylrA The structure of e.Coli nitroreductase with bound acetate, crystal form 1
    48% identity, 100% coverage of query (230 bits)

1ykiA The structure of e. Coli nitroreductase bound with the antibiotic nitrofurazone
    48% identity, 100% coverage of query (230 bits)

1oonA Nitroreductase from e-coli in complex with the dinitrobenzamide prodrug sn27217
    48% identity, 100% coverage of query (230 bits)

1icrA The structure of escherichia coli nitroreductase complexed with nicotinic acid
    48% identity, 100% coverage of query (230 bits)

nfnB / Q88K59 type 1 nitroaromatic nitroreductase from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 2 papers)
    50% identity, 99% coverage of query (220 bits)

FRA1_ALIFS / P46072 Major NAD(P)H-flavin oxidoreductase; FRASE I; EC 1.6.99.- from Aliivibrio fischeri (Vibrio fischeri) (see paper)
BAA04595.2 NAD(P)H-flavin oxidoreductase from Aliivibrio fischeri (see 2 papers)
    36% identity, 95% coverage of query (137 bits)

1v5yA / P46072 Binding of coumarins to NAD(p)h:fmn oxidoreductase
    36% identity, 95% coverage of query (137 bits)

1v5zA Binding of coumarins to NAD(p)h:fmn oxidoreductase
    36% identity, 95% coverage of query (137 bits)

rdxA / O25608 RdxA (EC 1.7.1.16) from Helicobacter pylori (strain ATCC 700392 / 26695) (see 6 papers)
RDXA_HELPY / O25608 Oxygen-insensitive NADPH nitroreductase; EC 1.-.-.- from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
    30% identity, 88% coverage of query (94.0 bits)

7jh4A / Q2FVA4 Crystal structure of NAD(p)h-flavin oxidoreductase (nfor) from s. Aureus complexed with reduced fmn and NAD+ (see paper)
    31% identity, 87% coverage of query (91.3 bits)

7jh4B Crystal structure of NAD(p)h-flavin oxidoreductase (nfor) from s. Aureus complexed with reduced fmn and NAD+
    31% identity, 87% coverage of query (91.3 bits)

Build an alignment

Build an alignment for Dsui_3297 and 25 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

7s14B / Q4QJZ7 Crystal structure of putative NAD(p)h-flavin oxidoreductase from haemophilus influenzae 86-028np
    29% identity, 90% coverage of query (89.4 bits)

7s14A Crystal structure of putative NAD(p)h-flavin oxidoreductase from haemophilus influenzae 86-028np
    29% identity, 90% coverage of query (89.4 bits)

7t33A The structure of haemophilus influenzae rd kw20 nitroreductase complexed with nicotinic acid
    28% identity, 90% coverage of query (89.4 bits)

Q81BW8 FMN reductase (NADPH) (EC 1.5.1.38) from Bacillus cereus (see paper)
    28% identity, 96% coverage of query (82.8 bits)

YFKO_BACSU / O34475 Putative NAD(P)H nitroreductase YfkO; EC 1.-.-.- from Bacillus subtilis (strain 168) (see paper)
    28% identity, 91% coverage of query (82.0 bits)

8q5gA / A0A6H0WK49 Crystal structure of nitroreductase from bacillus tequilensis with covalent fmn (see paper)
    28% identity, 90% coverage of query (80.1 bits)

3qdlA / O25608 Crystal structure of rdxa from helicobacter pyroli (see paper)
    27% identity, 88% coverage of query (79.0 bits)

6wt2A / A0A0U1RIB4 Crystal structure of putative NAD(p)h-flavin oxidoreductase from neisseria meningitidis
    30% identity, 87% coverage of query (76.3 bits)

3of4A / Q5R179 Crystal structure of a fmn/fad- and NAD(p)h-dependent nitroreductase (nfnb, il2077) from idiomarina loihiensis l2tr at 1.90 a resolution
    28% identity, 97% coverage of query (73.9 bits)

3of4C Crystal structure of a fmn/fad- and NAD(p)h-dependent nitroreductase (nfnb, il2077) from idiomarina loihiensis l2tr at 1.90 a resolution
    28% identity, 97% coverage of query (73.9 bits)

2h0uA / Q7BGI8 Crystal structure of NAD(p)h-flavin oxidoreductase from helicobacter pylori
    27% identity, 96% coverage of query (71.2 bits)

2hayD / Q9A120 The crystal structure of the putative NAD(p)h-flavin oxidoreductase from streptococcus pyogenes m1 gas
    29% identity, 90% coverage of query (64.3 bits)

3gbhB / A0A0H2VHN8 Crystal structure of a putative NAD(p)h:fmn oxidoreductase (se1966) from staphylococcus epidermidis atcc 12228 at 2.00 a resolution
    24% identity, 94% coverage of query (61.6 bits)

Q838N5 azobenzene reductase (EC 1.7.1.6) from Enterococcus faecalis (see paper)
    23% identity, 92% coverage of query (58.2 bits)

CLAER_LACPN / P0DX42 CLA biosynthesis enone reductase; CLA-ER; EC 1.3.1.- from Lactiplantibacillus plantarum (Lactobacillus plantarum) (see 2 papers)
4qlxA / U6C5W9 Crystal structure of cla-er with product binding (see paper)
    27% identity, 88% coverage of query (55.1 bits)

7vqkA / A0A3N6GWZ7 Catalytic manifolds of a fmn-dependent oxidoreductase rube7, expanding the functional diversity of the flavoenzyme superfamily (see paper)
    27% identity, 89% coverage of query (53.5 bits)

YODC_BACSU / P81102 Putative NAD(P)H nitroreductase YodC; EC 1.-.-.- from Bacillus subtilis (strain 168) (see paper)
    25% identity, 78% coverage of query (52.8 bits)

3ge6A / B1YG32 Crystal structure of a putative nitroreductase in complex with fmn (exig_2970) from exiguobacterium sibiricum 255-15 at 1.85 a resolution
    25% identity, 96% coverage of query (52.0 bits)

fre / A0A142JK69 FAD reductase (NADH) (EC 1.5.1.37) from Cupriavidus nantongensis (see paper)
    26% identity, 82% coverage of query (50.4 bits)

2b67A / A0A0H2UP38 Crystal structure of the nitroreductase family protein from streptococcus pneumoniae tigr4
    25% identity, 100% coverage of query (47.8 bits)

NFNB_MYCS2 / A0R6D0 Nitroreductase NfnB; NR NfnB; FMN-dependent NAD(P)H nitroreductase; EC 1.-.-.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
    26% identity, 99% coverage of query (47.8 bits)

2wzwA Crystal structure of the fmn-dependent nitroreductase nfnb from mycobacterium smegmatis in complex with NADPH
    26% identity, 95% coverage of query (47.8 bits)

2wzvB / A0R6D0 Crystal structure of the fmn-dependent nitroreductase nfnb from mycobacterium smegmatis (see paper)
    26% identity, 95% coverage of query (47.4 bits)

YcdI / b1008 putative malonic semialdehyde reductase (EC 1.1.1.298) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
rutE / P75894 putative malonic semialdehyde reductase (EC 1.1.1.298) from Escherichia coli (strain K12) (see 6 papers)
RUTE_ECOLI / P75894 Probable malonic semialdehyde reductase RutE; EC 1.1.1.298 from Escherichia coli (strain K12) (see 4 papers)
    23% identity, 88% coverage of query (44.7 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory