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Searching for up to 100 curated homologs for Echvi_4304 FitnessBrowser__Cola:Echvi_4304 (270 a.a.)

Found high-coverage hits (≥70%) to 59 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    44% identity, 95% coverage of query (217 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    46% identity, 91% coverage of query (213 bits)

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    36% identity, 95% coverage of query (169 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    36% identity, 95% coverage of query (169 bits)

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    35% identity, 94% coverage of query (166 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    36% identity, 93% coverage of query (164 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    36% identity, 91% coverage of query (163 bits)

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    36% identity, 92% coverage of query (159 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    32% identity, 96% coverage of query (142 bits)

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    33% identity, 77% coverage of query (116 bits)

Build an alignment

Build an alignment for Echvi_4304 and 10 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
    28% identity, 87% coverage of query (88.2 bits)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
    28% identity, 87% coverage of query (86.7 bits)

Q8KT44 carboxylesterase (EC 3.1.1.1) from Lacticaseibacillus casei (see paper)
    27% identity, 96% coverage of query (82.8 bits)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    27% identity, 96% coverage of query (77.0 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    27% identity, 96% coverage of query (77.0 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    27% identity, 96% coverage of query (77.0 bits)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    24% identity, 95% coverage of query (75.1 bits)

O73957 carboxylesterase (EC 3.1.1.1) from Sulfolobus acidocaldarius (see paper)
    27% identity, 73% coverage of query (74.7 bits)

ESTE_PSEFL / P22862 Arylesterase; Aryl-ester hydrolase; Carboxylic acid perhydrolase; PFE; Putative bromoperoxidase; EC 3.1.1.2; EC 1.-.-.- from Pseudomonas fluorescens (see 8 papers)
GI|951089 arylesterase; EC 1.-.-.-; EC 3.1.1.2 from Pseudomonas fluorescens (see 3 papers)
AAB60168.1 esterase from Pseudomonas fluorescens (see 2 papers)
    28% identity, 86% coverage of query (74.7 bits)

3heaA The l29p/l124i mutation of pseudomonas fluorescens esterase
    28% identity, 86% coverage of query (74.7 bits)

3hi4A Switching catalysis from hydrolysis to perhydrolysis in p. Fluorescens esterase
    28% identity, 86% coverage of query (74.7 bits)

3ia2A Pseudomonas fluorescens esterase complexed to the r-enantiomer of a sulfonate transition state analog
    28% identity, 86% coverage of query (74.7 bits)

8pi1B / P22862 Bicyclic incypro pseudomonas fluorescens esterase (see paper)
    28% identity, 86% coverage of query (73.6 bits)

P96965 2-hydroxymuconate-6-semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas fluorescens (see 2 papers)
cumD / BAA12150.1 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase from Pseudomonas fluorescens (see paper)
    26% identity, 89% coverage of query (71.2 bits)

1iunB / P96965 Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant hexagonal (see paper)
    26% identity, 89% coverage of query (70.1 bits)

2d0dA Crystal structure of a meta-cleavage product hydrolase (cumd) a129v mutant
    26% identity, 89% coverage of query (70.1 bits)

1ukaA Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with (s)-2-methylbutyrate
    26% identity, 89% coverage of query (70.1 bits)

1uk9A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with isovalerate
    26% identity, 89% coverage of query (70.1 bits)

1uk8A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-valerate
    26% identity, 89% coverage of query (70.1 bits)

1uk7A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-butyrate
    26% identity, 89% coverage of query (70.1 bits)

1iupA Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant complexed with isobutyrates
    26% identity, 89% coverage of query (70.1 bits)

5h3hB / K0ACL0 Esterase (eaest) from exiguobacterium antarcticum (see paper)
    28% identity, 71% coverage of query (66.2 bits)

cpoF / PDB|1A8S non-heme chloroperoxidase; EC 1.11.1.10 from Pseudomonas fluorescens (see 2 papers)
    27% identity, 86% coverage of query (65.1 bits)

A0A0H2WW38 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (EC 4.2.99.20) from Staphylococcus aureus (see paper)
    27% identity, 89% coverage of query (63.2 bits)

G3KFX4 2-hydroxymuconate-6-semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas sp. (see paper)
    26% identity, 88% coverage of query (62.8 bits)

xylF / P23106 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas putida (see paper)
XYLF_PSEPU / P23106 2-hydroxymuconate semialdehyde hydrolase; HMSH; 2-hydroxymuconic semialdehyde hydrolase; EC 3.7.1.9 from Pseudomonas putida (Arthrobacter siderocapsulatus)
    27% identity, 80% coverage of query (62.8 bits)

acoC / AAA21950.1 FMP from Cupriavidus necator (see paper)
    25% identity, 96% coverage of query (62.8 bits)

DCH_ACICA / Q83WC8 Bifunctional esterase/perhydrolase DCH; 3,4-dihydrocoumarin hydrolase; DCH; Metal-free haloperoxidase; EC 3.1.1.35 from Acinetobacter calcoaceticus (see 3 papers)
Q83WC8 dihydrocoumarin hydrolase (EC 3.1.1.35) from Acinetobacter calcoaceticus (see paper)
    28% identity, 87% coverage of query (62.8 bits)

nahN / BAE92169.1 2-hydroxymuconic semialdehyde hydrolase NahN from Pseudomonas putida (see 2 papers)
    28% identity, 88% coverage of query (61.6 bits)

hppC / AAB81313.1 2-hydroxy-6-ketonona-2,4-dienoate hydrolase from Rhodococcus globerulus (see paper)
    26% identity, 98% coverage of query (59.3 bits)

bphD / BAM76235.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Janibacter sp. TYM3221 (see paper)
    25% identity, 99% coverage of query (58.9 bits)

Q3HWU8 carboxylesterase (EC 3.1.1.1) from Pseudomonas putida (see paper)
    24% identity, 88% coverage of query (57.8 bits)

AGH13449.1 hybrid C-C meta-cleavage hydrolase-carboxylesterase from Cycloclasticus zancles (see paper)
    27% identity, 81% coverage of query (57.4 bits)

6ap6A / J9U5U9 Crystal structure of dad2 in complex with tolfenamic acid (see paper)
    23% identity, 83% coverage of query (54.7 bits)

BPHD_PSES1 / P17548 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; 2,6-dioxo-6-phenylhexa-3-enoate hydrolase; EC 3.7.1.8 from Pseudomonas sp. (strain KKS102) (see paper)
    27% identity, 79% coverage of query (54.7 bits)

6o5jA Crystal structure of dad2 bound to quinazolinone derivative
    23% identity, 83% coverage of query (54.7 bits)

6ap7A Crystal structure of dad2 in complex with 2-(2-methyl-3-nitroanilino) benzoic acid
    23% identity, 83% coverage of query (54.7 bits)

2ociA Crystal structure of valacyclovir hydrolase complexed with a product analogue
    22% identity, 94% coverage of query (54.7 bits)

2ocgA / Q86WA6 Crystal structure of human valacyclovir hydrolase (see paper)
    22% identity, 94% coverage of query (54.7 bits)

BPHL_HUMAN / Q86WA6 Valacyclovir hydrolase; VACVase; Valacyclovirase; Biphenyl hydrolase-like protein; Biphenyl hydrolase-related protein; Bph-rp; Breast epithelial mucin-associated antigen; MCNAA; EC 3.1.-.- from Homo sapiens (Human) (see 2 papers)
    22% identity, 94% coverage of query (54.7 bits)

dehH1 / Q01398 fluoroacetate dehalogenase monomer (EC 3.8.1.3) from Moraxella sp. (strain B) (see 2 papers)
dehH1 / RF|NP_904256.1 haloacetate dehalogenase H-1; EC 3.8.1.3 from Moraxella sp. B (see paper)
dehH1 / BAB85585.1 haloacetate dehalogenase H-1 from Delftia acidovorans (see 3 papers)
dehH1 / BAC81979.1 haloacetate dehalogenase H-1 from Delftia acidovorans (see 3 papers)
    23% identity, 79% coverage of query (53.9 bits)

DAD2_PETHY / J9U5U9 Probable strigolactone esterase DAD2; Protein DECREASED APICAL DOMINANCE 2; EC 3.1.-.- from Petunia hybrida (Petunia) (see paper)
    23% identity, 83% coverage of query (53.5 bits)

bphD / BAA25612.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus erythropolis (see paper)
    25% identity, 88% coverage of query (53.1 bits)

EstN1 / K0IAM1 pimeloyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Nitrososphaera gargensis (strain Ga9.2) (see paper)
    20% identity, 94% coverage of query (53.1 bits)

RSBQ_BACSU / O07015 Sigma factor SigB regulation protein RsbQ from Bacillus subtilis (strain 168) (see 2 papers)
    21% identity, 88% coverage of query (51.2 bits)

RMS3_PEA / A0A109QYD3 Strigolactone esterase RMS3; Protein DWARF 14 homolog; PsD14; Protein RAMOSUS 3; EC 3.1.-.- from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see paper)
    21% identity, 77% coverage of query (49.3 bits)

D14_ARATH / Q9SQR3 Strigolactone esterase D14; Protein DWARF 14; AtD14; EC 3.1.-.- from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
    22% identity, 71% coverage of query (48.1 bits)

5hzgA / Q9SQR3 The crystal structure of the strigolactone-induced atd14-d3-ask1 complex (see paper)
    22% identity, 74% coverage of query (47.8 bits)

7k38A Crystal structure of pisum sativum kai2 in complex with gr24-ent5ds product (see paper)
    19% identity, 75% coverage of query (45.4 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory