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Searching for up to 100 curated homologs for GFF1300 FitnessBrowser__WCS417:GFF1300 (417 a.a.)

Found high-coverage hits (≥70%) to 13 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Pf6N2E2_2924 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, permease component 2 LivM from Pseudomonas fluorescens FW300-N2E2
    87% identity, 100% coverage of query (707 bits)

TC 3.A.1.4.8 / P21628 High-affinity branched-chain amino acid transport system permease protein BraE, component of Branched chain amino acid uptake transporter. Transports alanine from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    74% identity, 100% coverage of query (556 bits)

LivM / b3456 branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM (EC 7.4.2.2) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
LivM / P22729 branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM (EC 7.4.2.2) from Escherichia coli (strain K12) (see 5 papers)
LIVM_ECOLI / P22729 High-affinity branched-chain amino acid transport system permease protein LivM; LIV-I protein M from Escherichia coli (strain K12) (see paper)
TC 3.A.1.4.1 / P22729 LivM aka B3456, component of Leucine; leucine/isoleucine/valine porter from Escherichia coli (see 5 papers)
    60% identity, 99% coverage of query (450 bits)

AZOBR_RS08240 L-proline and D-alanine ABC transporter, permease component 2 from Azospirillum brasilense Sp245
    63% identity, 76% coverage of query (403 bits)

braE / CAB75550.1 integral membrane protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    59% identity, 72% coverage of query (339 bits)

HSERO_RS00890 L-proline ABC transporter, permease component 2 from Herbaspirillum seropedicae SmR1
    40% identity, 78% coverage of query (242 bits)

TC 3.A.1.4.10 / Q8DQH9 ABC transporter membrane-spanning permease-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM from Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
    37% identity, 71% coverage of query (155 bits)

TC 3.A.1.4.3 / Q8VM84 BraE aka Bra2E, component of General L- (and D-)amino acid uptake porter (transports acidic, basic, polar, semipolar and hydrophobic amino acids). The amino and carboxyl groups do not need to be α since γ-aminobutyric acid (GABA) is a substrate. The system may function with additional binding proteins since L-alanine uptake is not dependent on BraC from Rhizobium leguminosarum (biovar viciae) (see paper)
    33% identity, 73% coverage of query (144 bits)

TC 3.A.1.4.6 / Q8YY08 NatC, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (see paper)
    31% identity, 71% coverage of query (113 bits)

Build an alignment

Build an alignment for GFF1300 and 9 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

TC 3.A.1.4.4 / Q9L3S4 UrtC, component of The high-affinity (<1 μM) urea porter (see paper)
    24% identity, 77% coverage of query (73.6 bits)

TC 3.A.1.4.5 / Q8NRV9 UrtC, component of The high affinity urea/thiourea/hydroxyurea porter from Corynebacterium glutamicum (see paper)
    25% identity, 71% coverage of query (69.7 bits)

AZOBR_RS08235 L-proline and D-alanine ABC transporter, permease component 1 from Azospirillum brasilense Sp245
    26% identity, 71% coverage of query (53.5 bits)

braD / CAB75549.1 integral membrane protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    24% identity, 71% coverage of query (48.1 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory