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Searching for up to 100 curated homologs for GFF136 FitnessBrowser__psRCH2:GFF136 (229 a.a.)

Found high-coverage hits (≥70%) to 40 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO356_09910 ABC transporter for L-Lysine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    78% identity, 100% coverage of query (368 bits)

TC 3.A.1.3.23 / Q9HU29 Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    70% identity, 99% coverage of query (328 bits)

TC 3.A.1.3.11 / O50183 AotP aka AotM aka PA0890, component of Arginine/ornithine (but not lysine) porter from Pseudomonas aeruginosa (see 2 papers)
    50% identity, 97% coverage of query (204 bits)

TC 3.A.1.3.6 / P35113 NocM aka ATU6025 aka AGR_PTI_65, component of Nopaline porter from Agrobacterium tumefaciens (see 3 papers)
    46% identity, 97% coverage of query (201 bits)

TC 3.A.1.3.5 / P35114 OCM1 aka OccM, component of Octopine porter from Agrobacterium tumefaciens (see 2 papers)
    49% identity, 94% coverage of query (200 bits)

Pf1N1B4_3433 ABC transporter for L-Arginine and L-Citrulline, permease component 2 from Pseudomonas fluorescens FW300-N1B4
    48% identity, 93% coverage of query (188 bits)

PS417_21735 ABC transporter for L-Arginine, permease component 2 from Pseudomonas simiae WCS417
    51% identity, 79% coverage of query (184 bits)

AO353_03045 ABC transporter for L-Arginine and L-Citrulline, permease component 1 from Pseudomonas fluorescens FW300-N2E3
    50% identity, 79% coverage of query (183 bits)

AO356_18710 L-Arginine ABC transporter, permease component 1 from Pseudomonas fluorescens FW300-N2C3
    51% identity, 79% coverage of query (182 bits)

Pf6N2E2_5662 L-Arginine ABC transporter, permease protein AotM from Pseudomonas fluorescens FW300-N2E2
    50% identity, 79% coverage of query (180 bits)

BPHYT_RS24010 L-histidine ABC transporter, permease component 2 from Burkholderia phytofirmans PsJN
    49% identity, 77% coverage of query (171 bits)

SMc00138 L-lysine ABC transporter, permease component 2 from Sinorhizobium meliloti 1021
    42% identity, 94% coverage of query (171 bits)

HISM_SALTY / P0A2I7 Histidine/lysine/arginine/ornithine transport system permease protein HisM from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 5 papers)
TC 3.A.1.3.1 / P0A2I7 Histidine transport system permease protein HisM aka STM2352, component of Histidine/arginine/lysine/ornithine porter (Heuveling et al. 2014). In contrast to some homologous homodimeric systems, the heterodimeric histidine transporter of Salmonella enterica Typhimurium from Salmonella typhimurium (see 3 papers)
    45% identity, 90% coverage of query (156 bits)

BPHYT_RS07680 ABC transporter for L-Arginine, permease component 1 from Burkholderia phytofirmans PsJN
    42% identity, 96% coverage of query (154 bits)

HisM / b2307 lysine/arginine/ornithine ABC transporter / histidine ABC transporter, membrane subunit HisM (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
HISM_ECOLI / P0AEU3 Histidine/lysine/arginine/ornithine transport system permease protein HisM from Escherichia coli (strain K12) (see paper)
TC 3.A.1.3.29 / P0AEU3 Histidine transport system permease protein HisM, component of Histidine/Arginine/Lysine (basic amino acid) uptake porter, HisJ/ArgT/HisP/HisM/HisQ [R, R, C, M, M, respectively] (Gilson et al. 1982). HisJ binds L-His (preferred), but 1-methyl-L-His and 3-methyl-L-His also bind, while the dipeptide carnosine binds weakly; D-histidine and the histidine degradation products, histamine, urocanic acid and imidazole do not bind. L-Arg, homo-L-Arg, and post-translationally modified methylated Arg-analogs also bind with the exception of symmetric dimethylated-L-Arg. L-Lys and L-Orn show weaker interactions with HisJ and methylated and acetylated Lys variants show poor binding.The carboxylate groups of these amino acids and their variants are essential from Escherichia coli (strain K12)
    45% identity, 90% coverage of query (154 bits)

AO356_05505 ABC transporter for L-Lysine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    42% identity, 91% coverage of query (149 bits)

ArtM / b0861 L-arginine ABC transporter membrane subunit ArtM (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see paper)
ArtM / P0AE30 L-arginine ABC transporter membrane subunit ArtM (EC 7.4.2.1) from Escherichia coli (strain K12) (see 2 papers)
ARTM_ECOLI / P0AE30 Arginine ABC transporter permease protein ArtM from Escherichia coli (strain K12) (see paper)
TC 3.A.1.3.3 / P0AE30 Arginine transport system permease protein ArtM aka B0861, component of Arginine porter from Escherichia coli (see 6 papers)
    40% identity, 93% coverage of query (148 bits)

Pf6N2E2_2960 ABC transporter for L-Lysine, permease component 2 from Pseudomonas fluorescens FW300-N2E2
    41% identity, 91% coverage of query (147 bits)

PP_3595 L-lysine and D-lysine ABC transporter, permease component 2 from Pseudomonas putida KT2440
    39% identity, 94% coverage of query (141 bits)

4ymtC / Q8RCC3 Crystal structure of an amino acid abc transporter complex with arginines (see paper)
    36% identity, 94% coverage of query (130 bits)

TcyL / b1918 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
tcyL / P0AFT2 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli (strain K12) (see 3 papers)
TCYL_ECOLI / P0AFT2 L-cystine transport system permease protein TcyL from Escherichia coli (strain K12) (see 5 papers)
TC 3.A.1.3.10 / P0AFT2 Inner membrane amino-acid ABC transporter permease protein YECS CysY aka B1918, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 4 papers)
    40% identity, 90% coverage of query (130 bits)

TC 3.A.1.3.17 / Q8YSA2 Basic amino acid uptake transporter, BgtAB (see paper)
    33% identity, 96% coverage of query (128 bits)

4ymwC Crystal structure of an amino acid abc transporter with histidines
    36% identity, 94% coverage of query (128 bits)

Ac3H11_2554 ABC transporter for L-Histidine, permease component 1 from Acidovorax sp. GW101-3H11
    33% identity, 86% coverage of query (126 bits)

ARTQ_BACSU / P54536 Arginine transport system permease protein ArtQ from Bacillus subtilis (strain 168) (see paper)
    35% identity, 95% coverage of query (121 bits)

TC 3.A.1.3.12 / P73544 BgtB aka GLNH aka SLL1270, component of Arginine/lysine/histidine/glutamine porter (see paper)
    38% identity, 92% coverage of query (121 bits)

TCYB_BACSU / P42200 L-cystine transport system permease protein TcyB from Bacillus subtilis (strain 168) (see paper)
TC 3.A.1.3.14 / P42200 TcyB (YckJ), component of Uptake system for L-cystine from Bacillus subtilis (see 4 papers)
    36% identity, 93% coverage of query (119 bits)

TC 3.A.1.3.25 / Q9CES5 Glutamine ABC transporter permease and substrate binding protein protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity from Lactococcus lactis subsp. lactis (strain IL1403)
    31% identity, 90% coverage of query (115 bits)

YXEN_BACSU / P54953 Probable amino-acid permease protein YxeN from Bacillus subtilis (strain 168) (see paper)
    34% identity, 96% coverage of query (111 bits)

GlnP / b0810 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
GlnP / P0AEQ6 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.3.2 / P0AEQ6 Glutamine transport system permease protein GlnP aka B0810, component of Three component ABC L-glutamine porter. The basal ATPase activity (ATP hydrolysis in the absence of substrate) is mainly caused by the docking of the closed-unliganded state of GlnH onto the transporter domain of GlnPQ. Unlike glutamine, arginine binds both GlnH domains, but does not trigger their closing. Comparison of the ATPase activity in nanodiscs with glutamine transport in proteoliposomes suggested that the stoichiometry of ATP per substrate is close to two from Escherichia coli (see 5 papers)
    31% identity, 93% coverage of query (104 bits)

Build an alignment

Build an alignment for GFF136 and 30 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Additional hits (identity < 30%)

GLNM_BACSU / O34671 Probable glutamine ABC transporter permease protein GlnM from Bacillus subtilis (strain 168) (see paper)
    29% identity, 97% coverage of query (103 bits)

TCYL_BACSU / O34315 L-cystine transport system permease protein TcyL from Bacillus subtilis (strain 168) (see 2 papers)
TC 3.A.1.3.13 / O34315 TcyL (YtmL), component of Uptake system for L-cystine (Km=2.5 μM), L-cystathionine, L-djenkolate ( 2-amino-3-[(2-amino-3-hydroxy-3-oxopropyl)sulfanylmethylsulfanyl] propanoic acid), and S-methyl-L-cysteine from Bacillus subtilis (see 4 papers)
    31% identity, 97% coverage of query (103 bits)

AO356_05500 ABC transporter for L-Lysine, permease component 1 from Pseudomonas fluorescens FW300-N2C3
    33% identity, 86% coverage of query (98.6 bits)

TC 3.A.1.3.22 / Q9I403 Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    32% identity, 94% coverage of query (98.2 bits)

Pf6N2E2_2959 ABC transporter for L-Lysine, permease component 1 from Pseudomonas fluorescens FW300-N2E2
    32% identity, 93% coverage of query (97.4 bits)

PP_3594 L-lysine and D-lysine ABC transporter, permease component 1 from Pseudomonas putida KT2440
    35% identity, 91% coverage of query (96.7 bits)

AO353_16285 ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 2 from Pseudomonas fluorescens FW300-N2E3
    32% identity, 94% coverage of query (96.3 bits)

TC 3.A.1.3.18 / Q8YPM7 NatH, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (see paper)
    35% identity, 90% coverage of query (95.9 bits)

AO353_21720 ABC transporter for D-glucosamine, permease component 2 from Pseudomonas fluorescens FW300-N2E3
    29% identity, 85% coverage of query (95.9 bits)

TCYM_BACSU / O34931 L-cystine transport system permease protein TcyM from Bacillus subtilis (strain 168) (see 2 papers)
TC 3.A.1.3.13 / O34931 TcyM (YtmM), component of Uptake system for L-cystine (Km=2.5 μM), L-cystathionine, L-djenkolate ( 2-amino-3-[(2-amino-3-hydroxy-3-oxopropyl)sulfanylmethylsulfanyl] propanoic acid), and S-methyl-L-cysteine from Bacillus subtilis (see 4 papers)
    31% identity, 88% coverage of query (95.5 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory