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Searching for up to 100 curated homologs for GFF1644 FitnessBrowser__Phaeo:GFF1644 (417 a.a.)

Found high-coverage hits (≥70%) to 28 curated proteins.

Removed hits that are identical to the query, leaving 27

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

MANI_THEFU / A0A077LPS9 D-mannose isomerase; EC 5.3.1.7 from Thermobifida fusca (Thermomonospora fusca)
    43% identity, 91% coverage of query (265 bits)

AO353_03400 D-mannose isomerase (EC 5.3.1.7) from Pseudomonas fluorescens FW300-N2E3
    37% identity, 96% coverage of query (242 bits)

SQUS_SALTY / Q8ZKT7 Sulfoquinovose isomerase; SQ isomerase; Sulfoquinovose-sulfofructose isomerase; SQ-SF isomerase; EC 5.3.1.31 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
    35% identity, 93% coverage of query (232 bits)

MANI_PSEC1 / A0A0P9JFY5 D-mannose isomerase; PsMIaseA; EC 5.3.1.7 from Pseudomonas cannabina pv. alisalensis (strain ATCC BAA-566 / CFBP 6866 / ICMP 15200 / BS91) (see paper)
    39% identity, 93% coverage of query (231 bits)

AO356_05200 D-mannose isomerase (EC 5.3.1.7) from Pseudomonas fluorescens FW300-N2C3
    39% identity, 96% coverage of query (231 bits)

SquS / b3880 sulfoquinovose isomerase (EC 5.3.1.31; EC 5.3.1.7) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
yihS / P32140 sulfoquinovose isomerase (EC 5.3.1.31; EC 5.3.1.7) from Escherichia coli (strain K12) (see 6 papers)
SQUS_ECOLI / P32140 Sulfoquinovose isomerase; SQ isomerase; Sulfoquinovose-sulfofructose isomerase; SQ-SF isomerase; EC 5.3.1.31 from Escherichia coli (strain K12) (see 3 papers)
yihS / RF|NP_418316 uncharacterized sugar isomerase yihS from Escherichia coli K12 (see paper)
    34% identity, 95% coverage of query (229 bits)

7ag4D / Q8ZKT7 Crystal structure of active site mutant of sq isomerase (yihs-h248a) from salmonella enterica in complex with sulfofructose (sf) (see paper)
    34% identity, 93% coverage of query (229 bits)

2zblA Functional annotation of salmonella enterica yihs-encoded protein
    34% identity, 93% coverage of query (229 bits)

PS417_22150 D-mannose isomerase (EC 5.3.1.7) from Pseudomonas simiae WCS417
    40% identity, 93% coverage of query (225 bits)

Pf1N1B4_597 D-mannose isomerase (EC 5.3.1.7) from Pseudomonas fluorescens FW300-N1B4
    38% identity, 95% coverage of query (223 bits)

MANI_MARM1 / F2JVT6 D-mannose isomerase; MI; EC 5.3.1.7 from Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1) (see paper)
    32% identity, 91% coverage of query (166 bits)

BPHYT_RS02050 D-mannose isomerase (EC 5.3.1.7) from Burkholderia phytofirmans PsJN
    32% identity, 92% coverage of query (160 bits)

Ac3H11_2078 D-mannose isomerase (EC 5.3.1.7) from Acidovorax sp. GW101-3H11
    31% identity, 83% coverage of query (140 bits)

HSERO_RS03650 Mannose isomerase (EC 5.3.1.7) from Herbaspirillum seropedicae SmR1
    30% identity, 85% coverage of query (131 bits)

Sama_0560 D-mannose isomerase (EC 5.3.1.7) from Shewanella amazonensis SB2B
    30% identity, 90% coverage of query (129 bits)

Build an alignment

Build an alignment for GFF1644 and 15 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

C6VW66 cellobiose epimerase (EC 5.1.3.11) from Dyadobacter fermentans (see 2 papers)
    22% identity, 83% coverage of query (79.3 bits)

8h1lB / A0A7U4E834 Crystal structure of glucose-2-epimerase in complex with d-glucitol from runella slithyformis runsl_4512 (see paper)
    23% identity, 80% coverage of query (74.7 bits)

D0MF61 cellobiose epimerase (EC 5.1.3.11) from Rhodothermus marinus (see paper)
    22% identity, 82% coverage of query (73.6 bits)

Dfer_5652 / C6VWU2 mannose 2-epimerase monomer (EC 5.1.3.44) from Dyadobacter fermentans (strain ATCC 700827 / DSM 18053 / CIP 107007 / KCTC 52180 / NS114) (see paper)
C6VWU2 mannose 2-epimerase (EC 5.1.3.44) from Runella slithyformis (see paper)
    23% identity, 80% coverage of query (70.9 bits)

3wkgA / F8WRK9 Crystal structure of cellobiose 2-epimerase in complex with glucosylmannose (see paper)
    22% identity, 82% coverage of query (70.1 bits)

CEEP_RHOMR / F8WRK9 Cellobiose 2-epimerase; CE; EC 5.1.3.11 from Rhodothermus marinus (Rhodothermus obamensis) (see paper)
F8WRK9 cellobiose epimerase (EC 5.1.3.11) from Rhodothermus marinus (see 5 papers)
    22% identity, 82% coverage of query (70.1 bits)

3wkiA Crystal structure of cellobiose 2-epimerase in complex with cellobiitol
    22% identity, 82% coverage of query (70.1 bits)

3wkhA Crystal structure of cellobiose 2-epimerase in complex with epilactose
    22% identity, 82% coverage of query (70.1 bits)

D2QDA5 cellobiose epimerase (EC 5.1.3.11) from Spirosoma linguale (see paper)
    21% identity, 85% coverage of query (65.5 bits)

A5FA14 cellobiose epimerase (EC 5.1.3.11) from Flavobacterium johnsoniae (see 2 papers)
    18% identity, 79% coverage of query (58.2 bits)

A9AYF3 cellobiose epimerase (EC 5.1.3.11) from Herpetosiphon aurantiacus (see paper)
    21% identity, 73% coverage of query (57.0 bits)

F5ITJ6 cellobiose epimerase (EC 5.1.3.11) from Dysgonomonas gadei (see paper)
    20% identity, 75% coverage of query (53.1 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory