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Searching for up to 100 curated homologs for GFF2493 FitnessBrowser__WCS417:GFF2493 (440 a.a.)

Found high-coverage hits (≥70%) to 48 curated proteins.

Removed hits that are identical to the query, leaving 47

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

TC 3.A.1.1.49 / O30491 MtlE, component of The polyol (mannitol, glucitol (sorbitol), arabitol (arabinitol; lyxitol)) uptake porter, MtlEFGK from Pseudomonas fluorescens
    97% identity, 100% coverage of query (859 bits)

mtlE / Q4KC57 polyol ABC-type transporter periplasmic substrate-binding protein from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) (see paper)
    85% identity, 100% coverage of query (788 bits)

MANBP_PSEAE / Q9I1E0 Mannitol-binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    79% identity, 94% coverage of query (699 bits)

AO356_00025 ABC transporter for D-Sorbitol, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2C3
    73% identity, 100% coverage of query (687 bits)

Pf6N2E2_1963 ABC transporter for D-sorbitol, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    73% identity, 100% coverage of query (686 bits)

4ryaA / B9JRF8 Crystal structure of abc transporter solute binding protein avi_3567 from agrobacterium vitis s4, target efi-510645, with bound d-mannitol
    65% identity, 94% coverage of query (562 bits)

BPHYT_RS16115 ABC transporter for D-Sorbitol, periplasmic substrate-binding component from Burkholderia phytofirmans PsJN
    61% identity, 100% coverage of query (551 bits)

TC 3.A.1.1.5 / O30831 SmoE, component of Hexitol (glucitol; mannitol) porter from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
    60% identity, 98% coverage of query (533 bits)

PGA1_c13210 ABC transporter for D-Sorbitol, periplasmic substrate-binding component from Phaeobacter inhibens BS107
    56% identity, 99% coverage of query (514 bits)

Ac3H11_2944 ABC transporter for D-Sorbitol, periplasmic substrate-binding component from Acidovorax sp. GW101-3H11
    59% identity, 94% coverage of query (508 bits)

Build an alignment

Build an alignment for GFF2493 and 10 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

HSERO_RS17000 ABC transporter for D-sorbitol/xylitol, substrate-binding component from Herbaspirillum seropedicae SmR1
    27% identity, 98% coverage of query (113 bits)

5iaiA / B9JM84 Crystal structure of abc transporter solute binding protein arad_9887 from agrobacterium radiobacter k84, target efi-510945 in complex with ribitol
    27% identity, 92% coverage of query (107 bits)

TC 3.A.1.1.7 / O51923 MalE aka PF1739, component of Maltose/trehalose porter (see paper)
    26% identity, 98% coverage of query (89.4 bits)

1eu8A / Q7LYW7 Structure of trehalose maltose binding protein from thermococcus litoralis (see paper)
    27% identity, 88% coverage of query (88.2 bits)

MALE_THELN / Q7LYW7 Trehalose/maltose-binding protein MalE; TMBP from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see 4 papers)
    26% identity, 98% coverage of query (86.7 bits)

smoF / A9CEY9 3-(6-sulfo-α-D-quinovosyl)-sn-glycerol binding protein from Agrobacterium fabrum (strain C58 / ATCC 33970) (see paper)
SMOF_AGRFC / A9CEY9 Sulfoquinovosyl glycerol-binding protein SmoF; SQGro-binding protein SmoF; SQ monooxygenase cluster protein F from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see 2 papers)
    27% identity, 75% coverage of query (84.7 bits)

4qrzA / A9CGI0 Crystal structure of sugar transporter atu4361 from agrobacterium fabrum c58, target efi-510558, with bound maltotriose
    25% identity, 90% coverage of query (73.6 bits)

4qsdA Crystal structure of atu4361 sugar transporter from agrobacterium fabrum c58, target efi-510558, with bound sucrose
    25% identity, 90% coverage of query (73.6 bits)

5ysdB / Q92AS8 Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotriose (see paper)
    25% identity, 83% coverage of query (73.2 bits)

Q92AS8 ABC-type beta-glucan transporter (EC 7.5.2.3) from Listeria innocua (see paper)
    25% identity, 83% coverage of query (73.2 bits)

5ysbA Crystal structure of beta-1,2-glucooligosaccharide binding protein in ligand-free form
    25% identity, 83% coverage of query (73.2 bits)

5ci5A / A8F7X5 Crystal structure of an abc transporter solute binding protein from thermotoga lettingae tmo (tlet_1705, target efi-510544) bound with alpha-d-tagatose
    24% identity, 75% coverage of query (72.8 bits)

5ysdA Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotriose
    25% identity, 83% coverage of query (72.8 bits)

SM_b21221 ABC transporter for D-Glucosamine, periplasmic substrate-binding protein from Sinorhizobium meliloti 1021
    25% identity, 97% coverage of query (70.9 bits)

6dtqA / G4XU73 Maltose bound t. Maritima male3 (see paper)
    24% identity, 89% coverage of query (69.7 bits)

TC 3.A.1.1.41 / G4FGN8 Extracellular solute-binding protein family 1, component of Trehalose porter. Also binds sucrose (Boucher and Noll, 2011). Induced by glucose and trehalose. Directly regulated by trehalose-responsive regulator TreR from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    24% identity, 89% coverage of query (69.7 bits)

Dshi_0547 ABC transporter for Xylitol, periplasmic substrate-binding component from Dinoroseobacter shibae DFL-12
    23% identity, 99% coverage of query (68.9 bits)

3jzjA / B0B0V1 Crystal structures of the gach receptor of streptomyces glaucescens gla.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of salmonella typhimurium. (see paper)
    24% identity, 73% coverage of query (67.0 bits)

3k02A Crystal structures of the gach receptor of streptomyces glaucescens gla.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of salmonella typhimurium.
    24% identity, 73% coverage of query (67.0 bits)

TC 3.A.1.1.16 / P58300 MalE aka PF1938, component of Maltooligosaccharide porter (Maltose is not a substrate, but maltotriose is.) from Pyrococcus furiosus (see paper)
    23% identity, 70% coverage of query (66.6 bits)

ELTP_MYCS2 / A0QXD9 Erythritol/L-threitol-binding protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
    22% identity, 75% coverage of query (64.7 bits)

SM_b21103 ABC transporter for L-Fucose, periplasmic substrate-binding protein from Sinorhizobium meliloti 1021
    26% identity, 75% coverage of query (63.2 bits)

7c68A Crystal structure of beta-glycosides-binding protein of abc transporter in a closed state bound to cellotetraose
    26% identity, 77% coverage of query (55.8 bits)

7c67A Crystal structure of beta-glycosides-binding protein of abc transporter in a closed state bound to cellotriose
    26% identity, 77% coverage of query (55.8 bits)

7c66A Crystal structure of beta-glycosides-binding protein of abc transporter in a closed state bound to cellobiose
    26% identity, 77% coverage of query (55.5 bits)

7c64A Crystal structure of beta-glycosides-binding protein of abc transporter in an open state (form ii)
    26% identity, 77% coverage of query (55.5 bits)

7c63A Crystal structure of beta-glycosides-binding protein of abc transporter in an open state (form i)
    26% identity, 77% coverage of query (55.5 bits)

6preA / A0A0H2URJ7 Sbp rafe in complex with verbascose (see paper)
    23% identity, 73% coverage of query (50.8 bits)

2i58A Crystal structure of rafe from streptococcus pneumoniae complexed with raffinose
    23% identity, 73% coverage of query (50.8 bits)

7c6kA Crystal structure of beta-glycosides-binding protein (w177x) of abc transporter in a closed state bound to cellotriose (form i)
    25% identity, 77% coverage of query (50.4 bits)

7c6gA / Q53W80 Crystal structure of beta-glycosides-binding protein (w177x) of abc transporter in an open-liganded state bound to gentiobiose (see paper)
    25% identity, 77% coverage of query (50.1 bits)

7c6lA Crystal structure of beta-glycosides-binding protein (w177x) of abc transporter in a closed state bound to cellotriose (form ii)
    25% identity, 77% coverage of query (50.1 bits)

7c6jB Crystal structure of beta-glycosides-binding protein (w177x) of abc transporter in a closed state bound to cellobiose
    25% identity, 77% coverage of query (50.1 bits)

7c6iB Crystal structure of beta-glycosides-binding protein (w177x) of abc transporter in an open-liganded state bound to sophorose
    25% identity, 77% coverage of query (50.1 bits)

TC 3.A.1.1.33 / Q8KN19 Putative sugar transporter sugar-binding protein, component of The N,N'-diacetylchitobiose uptake transporter, DasABC/MsiK (MsiK energizes several ABC transporters (see 3.A.1.1.23)) from Streptomyces sp. 139 (see 2 papers)
    21% identity, 72% coverage of query (46.6 bits)

UgpB / b3453 sn-glycerol 3-phosphate ABC transporter periplasmic binding protein (EC 7.6.2.10) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
UgpB / P0AG80 sn-glycerol 3-phosphate ABC transporter periplasmic binding protein (EC 7.6.2.10) from Escherichia coli (strain K12) (see 5 papers)
UGPB_ECOLI / P0AG80 sn-glycerol-3-phosphate-binding periplasmic protein UgpB; Bile-responsive chaperone from Escherichia coli (strain K12) (see 5 papers)
P0AG80 ABC-type glycerol 3-phosphate transporter (EC 7.6.2.10) from Escherichia coli (see paper)
TC 3.A.1.1.3 / P0AG80 sn-glycerol-3-phosphate-binding periplasmic protein UgpB aka B3453, component of Glycerol-phosphate porter. Transports both glycerol-3-P and glycerol-3-P diesters including glycerophosphocholine but not glycerol-2-P from Escherichia coli (see 4 papers)
ugpB / GB|AAC76478.1 sn-glycerol-3-phosphate-binding periplasmic protein ugpB from Escherichia coli (see 4 papers)
    24% identity, 88% coverage of query (45.8 bits)

4aq4A / P0AG80 Substrate bound sn-glycerol-3-phosphate binding periplasmic protein ugpb from escherichia coli (see paper)
    24% identity, 88% coverage of query (45.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory