Sites on a Tree

 

Searching for up to 100 curated homologs for GFF325 FitnessBrowser__WCS417:GFF325 (468 a.a.)

Found high-coverage hits (≥70%) to 72 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

GLN1B_PSETA / Q3V5W6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Pseudomonas taetrolens (see 2 papers)
    93% identity, 100% coverage of query (917 bits)

glnA / P0A9C5 glutamine synthetase (EC 6.3.1.2) from Escherichia coli (strain K12) (see 59 papers)
glnA glutamate--ammonia ligase; EC 6.3.1.2 from Escherichia coli K12 (see paper)
adenylyl-[glutamine synthetase] / ECOCYC|ADENYLYL-GS glutamine synthetase from Escherichia coli K12 (see paper)
    69% identity, 99% coverage of query (689 bits)

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12)
    69% identity, 99% coverage of query (689 bits)

GLN1B_SALTY / P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
P0A1P6 glutamine synthetase (EC 6.3.1.2) from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
glnA glutamate--ammonia ligase; EC 6.3.1.2 from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
    68% identity, 99% coverage of query (687 bits)

1f1hA / P0A1P6 Crystal structure of glutamine synthetase from salmonella typhimurium with thallium ions (see paper)
    68% identity, 99% coverage of query (684 bits)

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin
    68% identity, 99% coverage of query (684 bits)

glnA / CAA48234.1 glutamate--ammonia ligase from Proteus vulgaris (see paper)
    68% identity, 99% coverage of query (682 bits)

Reut_A2057 / Q46ZL2 3-(hydroxyamino)phenol mutase (EC 5.4.4.3) from Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (see paper)
3HAPM_CUPPJ / Q46ZL2 3-hydroxylaminophenol mutase; 3HAP mutase; 3-(hydroxyamino)phenol mutase; EC 5.4.4.3 from Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134)) (see paper)
    65% identity, 100% coverage of query (654 bits)

1lgrA / P0A1P6 Interactions of nucleotides with fully unadenylylated glutamine synthetase from salmonella typhimurium (see paper)
    63% identity, 99% coverage of query (622 bits)

2lgsA Feedback inhibition of fully unadenylylated glutamine synthetase from salmonella typhimurium by glycine, alanine, and serine
    63% identity, 99% coverage of query (622 bits)

glnA / AAA87025.1 glutamine synthetase, partial from Rhodobacter capsulatus (see 2 papers)
    59% identity, 93% coverage of query (535 bits)

GLN1B_SYNY3 / P77961 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see 3 papers)
P77961 glutamine synthetase (EC 6.3.1.2) from Synechocystis sp. (see paper)
    53% identity, 99% coverage of query (510 bits)

glnA / CAA78366.1 glutamine synthetase from Synechococcus sp. PCC 7002 (see paper)
    53% identity, 99% coverage of query (504 bits)

glnA / CAA59423.1 glutamine synthetase (GS), partial from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
    68% identity, 72% coverage of query (499 bits)

glnA / Q31L83 glutamine synthetase, type I from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (see paper)
    53% identity, 99% coverage of query (498 bits)

3ng0A / P77961 Crystal structure of glutamine synthetase from synechocystis sp. Pcc 6803
    52% identity, 99% coverage of query (496 bits)

GLN1B_NOSS1 / P00964 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
    52% identity, 99% coverage of query (491 bits)

Q9RHZ1 glutamine synthetase (EC 6.3.1.2) from Trichormus azollae (see paper)
    52% identity, 99% coverage of query (484 bits)

A0R079 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
    51% identity, 99% coverage of query (483 bits)

O85177 glutamine synthetase (EC 6.3.1.2) from Amycolatopsis mediterranei (see paper)
    52% identity, 99% coverage of query (469 bits)

GLN1B_STRCO / P15106 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
P15106 glutamine synthetase (EC 6.3.1.2) from Streptomyces coelicolor (see paper)
    51% identity, 99% coverage of query (460 bits)

5zlpJ / P94845 Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
    49% identity, 99% coverage of query (459 bits)

5zlpL Crystal structure of glutamine synthetase from helicobacter pylori
    49% identity, 99% coverage of query (459 bits)

5zlpA Crystal structure of glutamine synthetase from helicobacter pylori
    49% identity, 99% coverage of query (459 bits)

GLN1B_MYCBO / P0A591 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) (see 2 papers)
GLN1B_MYCTU / P9WN39 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 9 papers)
P0A591 glutamine synthetase (EC 6.3.1.2) from Mycobacterium tuberculosis variant bovis (see paper)
P9WN39 glutamine synthetase (EC 6.3.1.2) from Mycobacterium tuberculosis (see 12 papers)
    49% identity, 99% coverage of query (457 bits)

1htoA / P9WN39 Crystallographic structure of a relaxed glutamine synthetase from mycobacterium tuberculosis (see paper)
    49% identity, 99% coverage of query (457 bits)

4acfA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with imidazopyridine inhibitor ((4-(6-bromo-3- (butylamino)imidazo(1,2-a)pyridin-2-yl)phenoxy) acetic acid) and l- methionine-s-sulfoximine phosphate.
    49% identity, 99% coverage of query (457 bits)

3zxvA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with tri-substituted imidazole inhibitor (4-(2-tert-butyl- 4-(6-methoxynaphthalen-2-yl)-1h-imidazol-5-yl)pyridin-2-amine) and l- methionine-s-sulfoximine phosphate
    49% identity, 99% coverage of query (457 bits)

3zxrA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with tri-substituted imidazole inhibitor (3-(2-tert-butyl- 5-(pyridin-4-yl)-1h-imidazol-4-yl)quinoline) and l-methionine-s- sulfoximine phosphate.
    49% identity, 99% coverage of query (457 bits)

2bvcA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with a transition state mimic
    49% identity, 99% coverage of query (457 bits)

2whiA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with a purine analogue inhibitor and l-methionine-s- sulfoximine phosphate.
    49% identity, 99% coverage of query (456 bits)

4xycA Nanomolar inhibitors of mycobacterium tuberculosis glutamine synthetase 1: synthesis, biological evaluation and x-ray crystallographic studies
    48% identity, 99% coverage of query (439 bits)

2wgsA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with a purine analogue inhibitor.
    48% identity, 99% coverage of query (427 bits)

4s17A / A1A1Z1 The crystal structure of glutamine synthetase from bifidobacterium adolescentis atcc 15703
    48% identity, 98% coverage of query (419 bits)

GLNA_SULAC / Q9HH09 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
Q9HH09 glutamine synthetase (EC 6.3.1.2) from Sulfolobus acidocaldarius (see paper)
    43% identity, 99% coverage of query (373 bits)

GLNA_ARCFU / O29313 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
O29313 glutamine synthetase (EC 6.3.1.2) from Archaeoglobus fulgidus (see paper)
    39% identity, 100% coverage of query (308 bits)

GLN1A_BACSU / P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 9 papers)
P12425 glutamine synthetase (EC 6.3.1.2) from Bacillus subtilis (see 2 papers)
    42% identity, 99% coverage of query (301 bits)

4s0rD / P12425 Structure of gs-tnra complex (see paper)
    42% identity, 99% coverage of query (301 bits)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex
    42% identity, 99% coverage of query (301 bits)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex
    42% identity, 99% coverage of query (301 bits)

7tfaB / A0A0F0G8G2 7tfaB (see paper)
    40% identity, 99% coverage of query (297 bits)

GLNA3_HALMT / I3R176 Glutamine synthetase 3; GS3; Glutamate--ammonia ligase; EC 6.3.1.2 from Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) (see paper)
B8ZJH0 glutamine synthetase (EC 6.3.1.2) from Haloferax mediterranei (see paper)
    41% identity, 89% coverage of query (296 bits)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom
    40% identity, 99% coverage of query (296 bits)

8tfkA Glutamine synthetase
    41% identity, 99% coverage of query (294 bits)

GLNA1_METMA / Q8PY99 Glutamine synthetase; GS; Glutamate--ammonia ligase; EC 6.3.1.2 from Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) (see paper)
Q8PY99 glutamine synthetase (EC 6.3.1.2) from Methanosarcina mazei (see paper)
    41% identity, 99% coverage of query (293 bits)

P36205 glutamine synthetase (EC 6.3.1.2) from Thermotoga maritima (see paper)
    43% identity, 89% coverage of query (293 bits)

P36687 glutamine synthetase (EC 6.3.1.2) from Pyrococcus woesei (see paper)
    41% identity, 94% coverage of query (293 bits)

8ufjB / Q8PY99 8ufjB (see paper)
    41% identity, 99% coverage of query (292 bits)

GLNA_METJA / Q60182 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q60182 glutamine synthetase (EC 6.3.1.2) from Methanocaldococcus jannaschii (see paper)
    41% identity, 90% coverage of query (290 bits)

Q0E5H7 glutamine synthetase (EC 6.3.1.2) from Halobacillus halophilus (see paper)
    39% identity, 95% coverage of query (287 bits)

8ooxB / A0A832VZP6 8ooxB (see paper)
    38% identity, 98% coverage of query (286 bits)

GLNA_THEKO / O08467 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
O08467 glutamine synthetase (EC 6.3.1.2) from Pyrococcus sp. (see paper)
    42% identity, 91% coverage of query (285 bits)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
    37% identity, 99% coverage of query (282 bits)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus
    37% identity, 99% coverage of query (282 bits)

7tf9S / A0A5D5GA79 L. Monocytogenes gs(14)-q-glnr peptide (see paper)
    39% identity, 97% coverage of query (278 bits)

8oozA Glutamine synthetase
    37% identity, 98% coverage of query (278 bits)

7tenA Glutamine synthetase
    39% identity, 97% coverage of query (278 bits)

C4L2T5 glutamine synthetase (EC 6.3.1.2) from Exiguobacterium sp. (see paper)
    42% identity, 91% coverage of query (273 bits)

GLNA_THET8 / Q5SIP0 Glutamine synthetase; GS; Glutamate--ammonia ligase; EC 6.3.1.2 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
    38% identity, 99% coverage of query (269 bits)

7tdvC / E3VXC2 7tdvC (see paper)
    38% identity, 98% coverage of query (262 bits)

7tf6A Glutamine synthetase
    38% identity, 98% coverage of query (259 bits)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
    33% identity, 99% coverage of query (219 bits)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    32% identity, 99% coverage of query (219 bits)

7cqwA / A0A369R1N0 Gmas/adp complex-conformation 1 (see paper)
    31% identity, 90% coverage of query (148 bits)

7cquA Gmas/adp/metsox-p complex
    31% identity, 88% coverage of query (147 bits)

7cqqA Gmas in complex with amppnp and metsox
    31% identity, 88% coverage of query (147 bits)

7cqnA Gmas in complex with amppcp
    31% identity, 88% coverage of query (147 bits)

Build an alignment

Build an alignment for GFF325 and 67 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

5dm3C / Q1QZR8 Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
    28% identity, 83% coverage of query (118 bits)

5dm3A / Q1QZR8 Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
    27% identity, 82% coverage of query (103 bits)

GLUL / P15104 glutamine synthetase subunit (EC 6.3.1.2) from Homo sapiens (see paper)
GLNA_HUMAN / P15104 Glutamine synthetase; GS; Glutamate--ammonia ligase; Palmitoyltransferase GLUL; EC 6.3.1.2; EC 2.3.1.225 from Homo sapiens (Human) (see 7 papers)
P15104 glutamine synthetase (EC 6.3.1.2) from Homo sapiens (see 3 papers)
    27% identity, 71% coverage of query (63.5 bits)

2qc8A Crystal structure of human glutamine synthetase in complex with adp and methionine sulfoximine phosphate
    27% identity, 71% coverage of query (63.5 bits)

2ojwA Crystal structure of human glutamine synthetase in complex with adp and phosphate
    26% identity, 71% coverage of query (59.3 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory