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Searching for up to 100 curated homologs for GFF335 FitnessBrowser__WCS417:GFF335 (454 a.a.)

Found high-coverage hits (≥70%) to 41 curated proteins.

Removed hits that are identical to the query, leaving 40

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO353_12235 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Pseudomonas fluorescens FW300-N2E3
    87% identity, 100% coverage of query (826 bits)

Pf1N1B4_1591 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Pseudomonas fluorescens FW300-N1B4
    86% identity, 100% coverage of query (818 bits)

PfGW456L13_330 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Pseudomonas fluorescens GW456-L13
    86% identity, 100% coverage of query (817 bits)

hutF / Q4KJP1 formiminoglutamate deiminase subunit (EC 3.5.3.13) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) (see paper)
    85% identity, 100% coverage of query (802 bits)

AO356_09645 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Pseudomonas fluorescens FW300-N2C3
    84% identity, 100% coverage of query (796 bits)

Pf6N2E2_3808 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Pseudomonas fluorescens FW300-N2E2
    83% identity, 100% coverage of query (790 bits)

HUTF_PSEAE / Q9HU77 Formimidoylglutamate deiminase; Formiminoglutamate deiminase; N-formimino-L-glutamate deiminase; N-formimino-L-glutamate iminohydrolase; EC 3.5.3.13 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 3 papers)
Q9HU77 formimidoylglutamate deiminase (EC 3.5.3.13); formimidoylglutamase (EC 3.5.3.8) from Pseudomonas aeruginosa (see paper)
    76% identity, 100% coverage of query (689 bits)

4rdvB / Q9HU77 The structure of n-formimino-l-glutamate iminohydrolase from pseudomonas aeruginosa complexed with n-formimino-l-aspartate
    76% identity, 100% coverage of query (683 bits)

3mduA The structure of n-formimino-l-glutamate iminohydrolase from pseudomonas aeruginosa complexed with n-guanidino-l-glutamate
    76% identity, 99% coverage of query (682 bits)

SMc00673 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Sinorhizobium meliloti 1021
    55% identity, 99% coverage of query (481 bits)

4f0lB / Q2YIL4 Crystal structure of amidohydrolase from brucella melitensis
    49% identity, 99% coverage of query (412 bits)

Ac3H11_2549 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Acidovorax sp. GW101-3H11
    51% identity, 95% coverage of query (410 bits)

PGA1_c36360 Formiminoglutamic iminohydrolase (EC 3.5.3.13) from Phaeobacter inhibens BS107
    46% identity, 100% coverage of query (373 bits)

Build an alignment

Build an alignment for GFF335 and 13 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

PA0142 / Q9I6Z0 8-oxoguanine deaminase monomer (EC 3.5.4.32) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
OXODE_PSEAE / Q9I6Z0 8-oxoguanine deaminase; EC 3.5.4.32 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
Q9I6Z0 8-oxoguanine deaminase (EC 3.5.4.32) from Pseudomonas aeruginosa (see paper)
    25% identity, 86% coverage of query (90.5 bits)

8is4A / A0A4Q9D6T1 Structure of an isocytosine specific deaminase vcz in complexed with 5-fu (see paper)
    23% identity, 86% coverage of query (73.6 bits)

3lnpA / D3KFX9 Crystal structure of amidohydrolase family protein olei01672_1_465 from oleispira antarctica (see paper)
    24% identity, 90% coverage of query (72.8 bits)

4dzhA / Q8P8H1 Crystal structure of an adenosine deaminase from xanthomonas campestris (target nysgrc-200456) with bound zn
    25% identity, 88% coverage of query (72.0 bits)

dadD / Q58936 5'-deoxyadenosine deaminase monomer (EC 3.5.4.28; EC 3.5.4.4; EC 3.5.4.31; EC 3.5.4.41) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
DADD_METJA / Q58936 5'-deoxyadenosine deaminase; 5'-dA deaminase; 5'-methylthioadenosine deaminase; MTA deaminase; Adenosine deaminase; S-adenosylhomocysteine deaminase; SAH deaminase; EC 3.5.4.41; EC 3.5.4.31; EC 3.5.4.4; EC 3.5.4.28 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 2 papers)
Q58936 S-adenosylhomocysteine deaminase (EC 3.5.4.28); S-methyl-5'-thioadenosine deaminase (EC 3.5.4.31); adenosine deaminase (EC 3.5.4.4); 5'-deoxyadenosine deaminase (EC 3.5.4.41) from Methanocaldococcus jannaschii (see paper)
    21% identity, 93% coverage of query (68.6 bits)

3hpaA Crystal structure of an amidohydrolase gi:44264246 from an evironmental sample of sargasso sea (see paper)
    24% identity, 92% coverage of query (66.6 bits)

Q9HZ64 S-methyl-5'-thioadenosine deaminase (EC 3.5.4.31) from Pseudomonas aeruginosa (see paper)
    23% identity, 89% coverage of query (65.1 bits)

4dykA / Q9HZ64 Crystal structure of an adenosine deaminase from pseudomonas aeruginosa pao1 (target nysgrc-200449) with bound zn
    23% identity, 89% coverage of query (64.7 bits)

4gbdA Crystal structure of adenosine deaminase from pseudomonas aeruginosa pao1 with bound zn and methylthio-coformycin
    23% identity, 89% coverage of query (64.7 bits)

4f0rA / Q7NZ90 Crystal structure of an adenosine deaminase homolog from chromobacterium violaceum (target nysgrc-019589) bound zn and 5'- methylthioadenosine (unproductive complex)
    23% identity, 87% coverage of query (63.5 bits)

4f0sA Crystal structure of an adenosine deaminase homolog from chromobacterium violaceum (target nysgrc-019589) with bound inosine.
    23% identity, 87% coverage of query (63.5 bits)

trzN / Q8VS01 triazine hydrolase subunit (EC 3.8.1.8) from Nocardioides sp. C190 (see paper)
    26% identity, 76% coverage of query (63.2 bits)

trzN / A1RCJ9 triazine hydrolase (EC 3.8.1.8) from Paenarthrobacter aurescens (strain TC1) (see 3 papers)
    25% identity, 76% coverage of query (62.4 bits)

3lscA Crystal structure of the mutant e241q of atrazine chlorohydrolase trzn from arthrobacter aurescens tc1 complexed with zinc and atraton
    25% identity, 76% coverage of query (60.8 bits)

3lsbA Crystal structure of the mutant e241q of atrazine chlorohydrolase trzn from arthrobacter aurescens tc1 complexed with zinc and ametrin
    25% identity, 76% coverage of query (60.8 bits)

T1Z209 atrazine chlorohydrolase (EC 3.8.1.8) from Arthrobacter sp. DNS10 (see paper)
    25% identity, 75% coverage of query (60.5 bits)

5hmdA / A1RCJ9 Crystal structure of triazine hydrolase variant (y215h/e241q) (see paper)
    25% identity, 76% coverage of query (59.7 bits)

IXPDE_UNKP / P0CI72 Isoxanthopterin deaminase; EC 3.5.4.11 from Unknown prokaryotic organism (see paper)
P0CI72 pterin deaminase (EC 3.5.4.11) from unidentified prokaryotic organism (see paper)
    24% identity, 82% coverage of query (57.0 bits)

GDA / Q9Y2T3 guanine deaminase subunit (EC 3.5.4.45; EC 3.5.4.3) from Homo sapiens (see paper)
GUAD_HUMAN / Q9Y2T3 Guanine deaminase; Guanase; Guanine aminase; Guanine aminohydrolase; GAH; p51-nedasin; EC 3.5.4.3 from Homo sapiens (Human) (see 2 papers)
Q9Y2T3 guanine deaminase (EC 3.5.4.3) from Homo sapiens (see 5 papers)
    22% identity, 74% coverage of query (56.2 bits)

2uz9A / Q9Y2T3 Human guanine deaminase (guad) in complex with zinc and its product xanthine.
    22% identity, 74% coverage of query (56.2 bits)

4aqlA Human guanine deaminase in complex with valacyclovir
    23% identity, 74% coverage of query (56.2 bits)

atzB / P95442 hydroxyatrazine ethylaminohydrolase monomer (EC 3.5.4.43) from Pseudomonas sp. (strain ADP) (see 2 papers)
ATZB_PSESD / P95442 Hydroxydechloroatrazine ethylaminohydrolase; Hydroxyatrazine hydrolase; EC 3.5.4.43 from Pseudomonas sp. (strain ADP) (see paper)
    23% identity, 87% coverage of query (55.5 bits)

A1RCX5 hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.4.43) from Paenarthrobacter aurescens (see paper)
    23% identity, 87% coverage of query (55.1 bits)

mtaD / Q9X034 5-methylthioadenosine/S-adenosylhomocysteine deaminase (EC 3.5.4.31; EC 3.5.4.28) from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
MTAD_THEMA / Q9X034 5-methylthioadenosine/S-adenosylhomocysteine deaminase; MTA/SAH deaminase; EC 3.5.4.28; EC 3.5.4.31 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
Q9X034 S-methyl-5'-thioadenosine deaminase (EC 3.5.4.31) from Thermotoga maritima (see paper)
    23% identity, 89% coverage of query (51.2 bits)

1p1mA / Q9X034 Structure of thermotoga maritima amidohydrolase tm0936 bound to ni and methionine
    22% identity, 89% coverage of query (50.8 bits)

2plmA Crystal structure of the protein tm0936 from thermotoga maritima complexed with zn and s-inosylhomocysteine
    22% identity, 89% coverage of query (50.8 bits)

GUAD_MOUSE / Q9R111 Guanine deaminase; Guanase; Guanine aminase; Guanine aminohydrolase; GAH; EC 3.5.4.3 from Mus musculus (Mouse) (see paper)
    21% identity, 84% coverage of query (47.4 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory