Searching for up to 100 curated homologs for GFF3445 FitnessBrowser__psRCH2:GFF3445 (486 a.a.)
Found high-coverage hits (≥70%) to 91 curated proteins.
You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.
TC 2.A.22.5.3 / Q9KDT3 Neurotransmitter:sodium symporter of 455 aas, MhsT from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
43% identity, 92% coverage of query (310 bits)
6yu6B / Q9KDT3 Crystal structure of mhst in complex with l-leucine (see paper)
43% identity, 91% coverage of query (308 bits)
6yu7A Crystal structure of mhst in complex with l-tyrosine
43% identity, 91% coverage of query (308 bits)
6yu4A Crystal structure of mhst in complex with l-4f-phenylalanine
43% identity, 91% coverage of query (308 bits)
6yu5A Crystal structure of mhst in complex with l-valine
43% identity, 91% coverage of query (308 bits)
6yu3A Crystal structure of mhst in complex with l-phenylalanine
43% identity, 91% coverage of query (308 bits)
6yu2A Crystal structure of mhst in complex with l-isoleucine
43% identity, 91% coverage of query (308 bits)
4us3A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state
43% identity, 91% coverage of query (308 bits)
4us4A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state (lipidic cubic phase form)
43% identity, 89% coverage of query (301 bits)
Build an alignment for GFF3445 and 9 homologs with ≥ 30% identity
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TC 2.A.22.4.1 / O50649 High affinity tryptophan:Na+ symporter, TnaT, of 501 aas and 12 TMSs (Androutsellis-Theotokis et al., 2003). The Km for Tryptophan is 145 nM; tryptamine and serotonin weakly inhibited with Ki values of 200 and 440 μM, respectively. An evolutionarily conserved role of adjacent transmembrane segments 7 and 8 has been proposed from Symbiobacterium thermophilum (see 2 papers)
tnaT / BAD39427.1 sodium-and chloride-dependent transporter, TnaT from Symbiobacterium thermophilum IAM 14863 (see paper)
29% identity, 90% coverage of query (194 bits)
TC 2.A.22.5.1 / Q58715 Hypothetical Na+-dependent permease from Methanococcus jannaschii (see paper)
30% identity, 96% coverage of query (167 bits)
TC 2.A.22.5.2 / Q8RHM5 The 11 TMS Na+-dependent tyrosine transporter, Tyt1 from Fusobacterium nucleatum subsp. nucleatum (see paper)
29% identity, 85% coverage of query (162 bits)
TC 2.A.22.4.3 / Q8NRL8 Transporter, component of The methionine/alanine uptake porter, MetPS (Trotschel et al., 2008) (MetP is the transporter; MetS is an essential auxiliary subunit) from Corynebacterium glutamicum
33% identity, 80% coverage of query (161 bits)
3gwwA Leucine transporter leut in complex with s-fluoxetine
29% identity, 79% coverage of query (133 bits)
7wlwA X-ray structure of thermostabilized drosophila dopamine transporter with gaba transporter1-like substitutions in the binding site, in complex with skf89976a
25% identity, 93% coverage of query (133 bits)
7wgtA X-ray structure of thermostabilized drosophila dopamine transporter with gaba transporter1-like substitutions in the binding site, in complex with no711.
25% identity, 93% coverage of query (133 bits)
TC 2.A.22.4.2 / O67854 The amino acid (leucine):2 Na+ symporter, LeuTAa (Yamashita et al., 2005). LeuT possesses two ion binding sites, NA1 and NA2, both highly specific for Na+ but with differing mechanisms of binding (Noskov and Roux, 2008). X-ray structures have been determined for LeuT in substrate-free outward-open and apo inward-open states from Aquifex aeolicus (see paper)
29% identity, 80% coverage of query (132 bits)
2q6hA / O67854 Crystal structure analysis of leut complexed with l-leucine, sodium, and clomipramine (see paper)
29% identity, 79% coverage of query (132 bits)
3gwvA Leucine transporter leut in complex with r-fluoxetine
29% identity, 79% coverage of query (132 bits)
2qeiA Crystal structure analysis of leut complexed with l-alanine, sodium, and clomipramine
29% identity, 79% coverage of query (132 bits)
2q72A Crystal structure analysis of leut complexed with l-leucine, sodium, and imipramine
29% identity, 79% coverage of query (132 bits)
3gwuA Leucine transporter leut in complex with sertraline
29% identity, 79% coverage of query (132 bits)
3f4jA Crystal structure of leut bound to glycine and sodium
29% identity, 79% coverage of query (132 bits)
3f3dA Crystal structure of leut bound to l-methionine and sodium
29% identity, 79% coverage of query (132 bits)
3f3cA Crystal structure of leut bound to 4-fluoro-l-phenylalanine and sodium
29% identity, 79% coverage of query (132 bits)
2qb4A Crystal structure analysis of leut complexed with l-leucine, sodium and desipramine
29% identity, 79% coverage of query (132 bits)
3uspA Crystal structure of leut in heptyl-beta-d-selenoglucoside
29% identity, 79% coverage of query (132 bits)
4xpfA X-ray structure of drosophila dopamine transporter with subsiteb mutations (d121g/s426m) bound to rti-55
24% identity, 93% coverage of query (131 bits)
3f3aA Crystal structure of leut bound to l-tryptophan and sodium
29% identity, 79% coverage of query (131 bits)
3uslA Crystal structure of leut bound to l-selenomethionine in space group c2 from lipid bicelles
29% identity, 79% coverage of query (131 bits)
3usgA Crystal structure of leut bound to l-leucine in space group c2 from lipid bicelles
29% identity, 79% coverage of query (131 bits)
3mpnA F177r1 mutant of leut
29% identity, 79% coverage of query (130 bits)
4xpgA X-ray structure of drosophila dopamine transporter with subsiteb mutations (d121g/s426m) bound to beta-cft or win35428
25% identity, 93% coverage of query (130 bits)
6m38A X-ray structure of a drosophila dopamine transporter with subsiteb mutations (d121g/s426m) in s-duloxetine bound form
25% identity, 93% coverage of query (129 bits)
4xnuA X-ray structure of drosophila dopamine transporter in complex with nisoxetine
25% identity, 93% coverage of query (129 bits)
4m48A X-ray structure of dopamine transporter elucidates antidepressant mechanism
25% identity, 93% coverage of query (129 bits)
4xp9C / Q7K4Y6 X-ray structure of drosophila dopamine transporter bound to psychostimulant d-amphetamine (see paper)
25% identity, 93% coverage of query (128 bits)
4xpaA X-ray structure of drosophila dopamine transporter bound to 3, 4dichlorophenethylamine
25% identity, 93% coverage of query (128 bits)
4xp6A X-ray structure of drosophila dopamine transporter bound to psychostimulant methamphetamine
25% identity, 93% coverage of query (128 bits)
4xp5A X-ray structure of drosophila dopamine transporter bound to cocaine analogue-rti55
25% identity, 93% coverage of query (128 bits)
4xp4A X-ray structure of drosophila dopamine transporter in complex with cocaine
25% identity, 93% coverage of query (128 bits)
4xnxA X-ray structure of drosophila dopamine transporter in complex with reboxetine
25% identity, 93% coverage of query (128 bits)
4xp1A X-ray structure of drosophila dopamine transporter bound to neurotransmitter dopamine
25% identity, 93% coverage of query (128 bits)
8gnkA Cryoem structure of cytosol-facing, substrate-bound ratgat1
25% identity, 89% coverage of query (128 bits)
6m3zA X-ray structure of a drosophila dopamine transporter with net-like mutations (d121g/s426m/f471l) in milnacipran bound form
25% identity, 93% coverage of query (127 bits)
6m0zA X-ray structure of drosophila dopamine transporter with net-like mutations (d121g/s426m/f471l) in l-norepinephrine bound form
25% identity, 93% coverage of query (127 bits)
6m47A X-ray structure of a drosophila dopamine transporter with net-like mutations (d121g/s426m/f471l) in tramadol bound form
25% identity, 93% coverage of query (127 bits)
6xwmA Mechanism of substrate release in neurotransmitter:sodium symporters: the structure of leut in an inward-facing occluded conformation
29% identity, 78% coverage of query (125 bits)
SC6A1_HUMAN / P30531 Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1 from Homo sapiens (Human) (see 2 papers)
TC 2.A.22.3.2 / P30531 γ-aminobutyric acid (GABA):Na+:Cl- symporter, GAT-1 (Stoichiometry, GABA:Na+ = 1:2 where both Na+ binding sites, Na1 and Na2, have been identified. Na2 but not Na1 can accommodate Li+ (Zhou et al., 2006)). Cai et al. 2005 have reported that N-glycosylation increases the stability, trafficking and GABA-uptake of GABA transporter 1. Glutamine 291 is essential for Cl- binding (Ben-Yona et al., 2011). Four human isoforms have been identified, GAT-1, GAT-2, GAT-3, and GAT-4, all about 70% identical to each other (Borden et al., 1992). GAT-2 transports γ-aminobutyric acid and β-alanine (Christiansen et al, 2007) It also concentratively takes up β-alanine and α-fluoro-β-alanine (Liu et al., 1999). GAT1 is capable of intracellular Na+-, Cl-- and GABA-induced outward currents (reverse GABA transport; GABA efflux) from Homo sapiens (Human) (see 3 papers)
26% identity, 79% coverage of query (123 bits)
SC6A1_RAT / P23978 Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1 from Rattus norvegicus (Rat) (see 4 papers)
SC6A1_MOUSE / P31648 Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1 from Mus musculus (Mouse) (see 4 papers)
25% identity, 79% coverage of query (122 bits)
7sk2A / P30531 Human wildtype gaba reuptake transporter 1 in complex with tiagabine, inward-open conformation (see paper)
27% identity, 78% coverage of query (122 bits)
SC6A1_MUSCO / P48057 Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1 from Mus cookii (Cook's mouse) (see paper)
25% identity, 79% coverage of query (122 bits)
4mmeA Crystal structure of leubat (delta6 mutant) in complex with mazindol
28% identity, 79% coverage of query (121 bits)
INE_DROME / Q9VR07 Sodium- and chloride-dependent GABA transporter ine; Protein inebriated; Protein receptor oscillation A from Drosophila melanogaster (Fruit fly) (see 6 papers)
TC 2.A.22.3.12 / Q9VR07 Sodium- and chloride-dependent GABA transporter, Ine (Protein inebriated) (Protein receptor oscillation A) from Drosophila melanogaster (see 10 papers)
25% identity, 79% coverage of query (120 bits)
4mm7A Crystal structure of leubat (delta13 mutant) in complex with desvenlafaxine
28% identity, 79% coverage of query (120 bits)
4mm4B Crystal structure of leubat (delta13 mutant) in complex with paroxetine
28% identity, 79% coverage of query (120 bits)
4mm4A Crystal structure of leubat (delta13 mutant) in complex with paroxetine
28% identity, 79% coverage of query (120 bits)
4mm9A Crystal structure of leubat (delta13 mutant) in complex with fluvoxamine
28% identity, 79% coverage of query (120 bits)
4mm6A Crystal structure of leubat (delta13 mutant) in complex with (s)- duloxetine
28% identity, 79% coverage of query (120 bits)
7y7wA Cryo-em structure of human gaba transporter gat1 bound with gaba in nacl solution in an inward-occluded state at 2.4 angstrom
27% identity, 78% coverage of query (120 bits)
DAT_DROME / Q7K4Y6 Sodium-dependent dopamine transporter; Protein fumin from Drosophila melanogaster (Fruit fly) (see 6 papers)
TC 2.A.22.1.7 / Q7K4Y6 Dopamine transporter from Drosophila melanogaster
23% identity, 93% coverage of query (119 bits)
7y7yA Cryo-em structure of human gaba transporter gat1 bound with nipecotic acid in nacl solution in an inward-occluded state at 2.4 angstrom
26% identity, 78% coverage of query (118 bits)
S6A13_RAT / P31646 Sodium- and chloride-dependent GABA transporter 2; GAT-2; Solute carrier family 6 member 13 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 92% coverage of query (117 bits)
S6A13_MOUSE / P31649 Sodium- and chloride-dependent GABA transporter 2; GAT-2; Sodium- and chloride-dependent GABA transporter 3; GAT-3; Solute carrier family 6 member 13 from Mus musculus (Mouse) (see 3 papers)
TC 2.A.22.3.8 / P31649 Mouse GABA, β-alanine, fluoro-β-alanine and taurine transporter-3 (GAT3) (Liu et al. 1999). Orthologous to rat and human GAT2; 72% identical to GAT4 (2.A.22.3.7) (takes up GABA with high affinity into presynaptic terminals). Also takes up the carnitine precursor, gamma-butyrobetaine from Mus musculus (see 3 papers)
25% identity, 92% coverage of query (117 bits)
S6A13_HUMAN / Q9NSD5 Sodium- and chloride-dependent GABA transporter 2; GAT-2; Solute carrier family 6 member 13 from Homo sapiens (Human) (see 3 papers)
TC 2.A.22.3.10 / Q9NSD5 Sodium- and chloride-dependent GABA transporter 2 (GAT-2) (Solute carrier family 6 member 13) from Homo sapiens (see 5 papers)
24% identity, 89% coverage of query (112 bits)
TC 2.A.22.2.9 / B2ZXL8 The densovirus type-2 (BmDNV-2) receptor; putative amino acid transporter, the densonucleosis refractoriness, Nsd-2 protein, of 625 aas and 11-12 TMSs (Abe et al. 2000). Deletion of the nsd2 gene, encoding this transporter in the midgut membrane, causes resistance to this parvo-like virus as well as bidensovirus from Bombyx mori (see paper)
24% identity, 91% coverage of query (107 bits)
S6A12_MOUSE / P31651 Sodium- and chloride-dependent betaine transporter; BGT-1; Na(+)/Cl(-) betaine/GABA transporter; Sodium- and chloride-dependent GABA transporter 2; GAT-2; Solute carrier family 6 member 12 from Mus musculus (Mouse) (see paper)
24% identity, 93% coverage of query (106 bits)
8de4A Native serotonin transporter in complex with 15b8 fab in the presence of methamphetamine
25% identity, 72% coverage of query (103 bits)
8de3A Native serotonin transporter in complex with 15b8 fab antibody in the presence of cocaine
25% identity, 72% coverage of query (103 bits)
SNF3_CAEEL / G5EBN9 Sodium- and chloride-dependent betaine transporter; Betaine transporter SNF-3; Na(+)/Cl(-) betaine/GABA transporter; Sodium:neurotransmitter symporter family protein 3 from Caenorhabditis elegans (see 2 papers)
22% identity, 71% coverage of query (102 bits)
NTDO_CAEEL / Q03614 Sodium-dependent dopamine transporter; DA transporter; DAT from Caenorhabditis elegans (see 3 papers)
TC 2.A.22.1.4 / Q03614 Antidepressant- and cocaine-sensitive dopamine transporter, T23G5.5 (Km for dopamine, 1.2 µM; dependent on extracellular Na+ and Cl-; blocked by cocaine and D-amphetamine) (Jayanthi et al. 1998) from Caenorhabditis elegans (see 3 papers)
23% identity, 73% coverage of query (100 bits)
S6A12_HUMAN / P48065 Sodium- and chloride-dependent betaine transporter; BGT-1; Na(+)/Cl(-) betaine/GABA transporter; Solute carrier family 6 member 12 from Homo sapiens (Human) (see paper)
TC 2.A.22.3.1 / P48065 Betaine/GABA:Na+ symporter, BGT1. (Substrates include: betaine, GABA, diaminobutyrate, β-alanine, proline, quinidine, dimethylglycine, glycine, and sarcosine with decreasing affinity in that order) from Homo sapiens (Human) (see 5 papers)
23% identity, 93% coverage of query (99.4 bits)
SC6A2_HUMAN / P23975 Sodium-dependent noradrenaline transporter; Norepinephrine transporter; NET; Solute carrier family 6 member 2 from Homo sapiens (Human) (see 5 papers)
TC 2.A.22.1.2 / P23975 Noradrenaline (norepinephrine):Na+ symporter (NET1, NAT1, SLC6?A2) (also transports 1-methyl-4-tetrahydropyridinium and amphetamines; it is a target of cocaine and amphetamines as well as of therapetics for depression, obsessive-compulsive disorders, and post-traumatic stress disorder. This homooligomeric transporter binds one substrate molecule per transporter subunit (Schwartz et al., 2005; Schlessinger et al., 2011; Andersen et al., 2011). Extracellular loop 3 contributes to substrate and inhibitor selectivity (Lynagh et al. 2013). The highly conserved MELAL and GQXXRXG motifs, located in the second transmembrane domain and the first intracellular loop of hNET, respectively, are determinants of NET cell surface expression, and substrate and inhibitor binding (Sucic and Bryan-Lluka 2007). Based on modeling, the high affinity substrate binding site (S1) of the human norepinephrine transporter has been predicted and then verified by mutational studies from Homo sapiens (Human) (see 5 papers)
SLC6A2 / RF|NP_001034.1 sodium-dependent noradrenaline transporter from Homo sapiens
23% identity, 74% coverage of query (98.2 bits)
SC6A2_MOUSE / O55192 Sodium-dependent noradrenaline transporter; Norepinephrine transporter; NET; Solute carrier family 6 member 2 from Mus musculus (Mouse) (see paper)
23% identity, 75% coverage of query (97.8 bits)
SC6A2_BOVIN / P51143 Sodium-dependent noradrenaline transporter; Norepinephrine transporter; NET; Solute carrier family 6 member 2 from Bos taurus (Bovine) (see paper)
23% identity, 74% coverage of query (97.4 bits)
SC6A3_RAT / P23977 Sodium-dependent dopamine transporter; DA transporter; DAT; Solute carrier family 6 member 3 from Rattus norvegicus (Rat) (see 6 papers)
25% identity, 72% coverage of query (94.7 bits)
SC6A4_DROME / P51905 Sodium-dependent serotonin transporter; 5HT transporter; 5HTT; Cocaine-sensitive serotonin transporter; dSERT1 from Drosophila melanogaster (Fruit fly) (see 2 papers)
TC 2.A.22.1.9 / P51905 The sodium-dependent serotonin transporter of 622 aas and 12 TMSs, SERT or SerT from Drosophila melanogaster
25% identity, 74% coverage of query (94.0 bits)
SC6A3_MOUSE / Q61327 Sodium-dependent dopamine transporter; DA transporter; DAT; Solute carrier family 6 member 3 from Mus musculus (Mouse) (see 6 papers)
25% identity, 72% coverage of query (94.0 bits)
SC6A7_HUMAN / Q99884 Sodium-dependent proline transporter; Solute carrier family 6 member 7 from Homo sapiens (Human) (see paper)
TC 2.A.22.2.11 / Q99884 Sodium-dependent proline transporter (Solute carrier family 6 member 7) from Homo sapiens (see 3 papers)
25% identity, 73% coverage of query (92.8 bits)
TC 2.A.22.1.5 / Q95VZ4 High affinity octopamine transporter, OAT (also transports tyramine and dopamine in the 0.4-3.0 μM range (Donly et al., 2007)) from Trichoplusia ni (see paper)
24% identity, 90% coverage of query (92.4 bits)
SC6A7_RAT / P28573 Sodium-dependent proline transporter; Solute carrier family 6 member 7 from Rattus norvegicus (Rat) (see paper)
TC 2.A.22.2.1 / P28573 Proline:Na+ symporter from Rattus norvegicus (Rat) (see paper)
Slc6a7 / RF|NP_446448.2 sodium-dependent proline transporter from Rattus norvegicus
25% identity, 73% coverage of query (92.4 bits)
S6A12_CANLF / P27799 Sodium- and chloride-dependent betaine transporter; Na(+)/Cl(-) betaine/GABA transporter; Solute carrier family 6 member 12 from Canis lupus familiaris (Dog) (Canis familiaris) (see 3 papers)
23% identity, 93% coverage of query (89.4 bits)
AAT07965.1 tryptophan transporter from Anopheles gambiae (see paper)
22% identity, 78% coverage of query (88.2 bits)
SC6A6_CANLF / Q00589 Sodium- and chloride-dependent taurine transporter; Solute carrier family 6 member 6 from Canis lupus familiaris (Dog) (Canis familiaris) (see 2 papers)
26% identity, 74% coverage of query (86.7 bits)
SC6A6_RAT / P31643 Sodium- and chloride-dependent taurine transporter; Solute carrier family 6 member 6 from Rattus norvegicus (Rat) (see 3 papers)
23% identity, 79% coverage of query (85.9 bits)
SC6A6_HUMAN / P31641 Sodium- and chloride-dependent taurine transporter; Solute carrier family 6 member 6 from Homo sapiens (Human) (see 5 papers)
TC 2.A.22.3.3 / P31641 The taurine:Na+ symporter, TauT or SLC6A6 (also transports β-alanine and γ-aminobutyric acid (GABA); Tomi et al., 2008; Anderson et al., 2009). Biallelic mutation of TauT is linked to early retinal degeneration (Preising et al. 2019). Oral taurine administration of retinal degeneration and cardiomyopathy reverses the phenotype from Homo sapiens (Human) (see 4 papers)
24% identity, 79% coverage of query (84.0 bits)
AAY56384.1 sodium-dependent nutrient amino acid transporter 1 from Drosophila melanogaster (see paper)
24% identity, 91% coverage of query (79.7 bits)
NAAT1_DROME / Q9W4C5 Sodium-dependent nutrient amino acid transporter 1; DmNAT1 from Drosophila melanogaster (Fruit fly) (see paper)
TC 2.A.22.2.13 / Q9W4C5 Sodium-dependent nutrient amino acid transporter 1 (DmNAAT1) from Drosophila melanogaster (see 5 papers)
24% identity, 91% coverage of query (79.3 bits)
TC 2.A.22.1.10 / Q963F3 Serotonin transporter, Mod-5, of 671 aas and 12 TMSs from Caenorhabditis elegans
23% identity, 76% coverage of query (76.6 bits)
SNF6_CAEEL / O76689 Sodium-dependent acetylcholine transporter; Sodium:neurotransmitter symporter family protein 6 from Caenorhabditis elegans (see paper)
21% identity, 93% coverage of query (75.9 bits)
TC 2.A.22.2.7 / O76689 Acetylcholine/choline:Na+ symporter, Snf-6 (interacts with dystrophin which determines its localization to the neuromuscular junction) from Caenorhabditis elegans (see 2 papers)
snf-6 / RF|NP_497416.4 sodium-dependent acetylcholine transporter from Caenorhabditis elegans
21% identity, 93% coverage of query (74.3 bits)
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