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Searching for up to 100 curated homologs for GFF428 FitnessBrowser__WCS417:GFF428 (494 a.a.)

Found high-coverage hits (≥70%) to 63 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

TC 2.A.21.2.3 / Q9I5F5 L-proline uptake porter, PutP from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    84% identity, 99% coverage of query (824 bits)

Psest_3078 L-proline:Na+ symporter from Pseudomonas stutzeri RCH2
    74% identity, 99% coverage of query (747 bits)

PutC / b1015 proline:Na+ symporter from Escherichia coli K-12 substr. MG1655 (see 7 papers)
putP / P07117 proline:Na+ symporter from Escherichia coli (strain K12) (see 12 papers)
PUTP_ECOLI / P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 9 papers)
TC 2.A.21.2.1 / P07117 Proline:Na+ symporter, PutP from Escherichia coli (see 12 papers)
    76% identity, 99% coverage of query (735 bits)

PUTP_SALTY / P10502 Sodium/proline symporter; Proline permease from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
    76% identity, 99% coverage of query (734 bits)

PUTP_BACSU / P94392 High-affinity proline transporter PutP from Bacillus subtilis (strain 168) (see 3 papers)
TC 2.A.21.2.4 / J7JIE8 The high affinity nutritional proline uptake porter, PutP from Bacillus subtilis QB928
    55% identity, 96% coverage of query (506 bits)

OPUE_BACSU / O06493 Osmoregulated proline transporter OpuE; Osmoprotectant uptake; Proline uptake system from Bacillus subtilis (strain 168) (see 2 papers)
TC 2.A.21.2.5 / M1TZ79 Proline uptake porter, OpuE (YerK) from Bacillus subtilis subsp. subtilis 6051-HGW
opuE / AAB72182.1 OpuE from Bacillus subtilis (see paper)
    50% identity, 100% coverage of query (477 bits)

PUTP_STAA8 / Q2FWY7 Sodium/proline symporter; Proline permease from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see 3 papers)
TC 2.A.21.2.2 / Q7A4Q7 Sodium/proline symporter (Proline permease) from Staphylococcus aureus (strain N315) (see paper)
    51% identity, 96% coverage of query (458 bits)

TC 2.A.21.2.6 / O24896 High affinity proline-specific Na+:proline symporter, PutP (Rivera-Ordaz et al. 2013).  Proline is a preferred source of energy for this microaerophilic bacterium.  PutP is efficiently inhibited by the proline analogs, 3,4-dehydro-D,L-proline and L-azetidine-2-carboxylic acid from Helicobacter pylori (strain ATCC 700392 / 26695)
    49% identity, 99% coverage of query (456 bits)

putP / AAA79365.1 proline permease homolog from Staphylococcus aureus (see paper)
    52% identity, 94% coverage of query (452 bits)

Build an alignment

Build an alignment for GFF428 and 9 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

YjcG / b4067 acetate/glycolate:cation symporter from Escherichia coli K-12 substr. MG1655 (see 2 papers)
actP / P32705 acetate/glycolate:cation symporter from Escherichia coli (strain K12) (see 5 papers)
ACTP_ECOLI / P32705 Cation/acetate symporter ActP; Acetate permease; Acetate transporter ActP from Escherichia coli (strain K12) (see 2 papers)
TC 2.A.21.7.2 / P32705 Acetate/glyoxylate permease, ActP from Escherichia coli (see 6 papers)
    26% identity, 88% coverage of query (127 bits)

PanF / b3258 pantothenate:Na+ symporter from Escherichia coli K-12 substr. MG1655 (see 3 papers)
panF / P16256 pantothenate:Na+ symporter from Escherichia coli (strain K12) (see 3 papers)
PANF_ECOLI / P16256 Sodium/pantothenate symporter; Pantothenate permease from Escherichia coli (strain K12) (see 3 papers)
TC 2.A.21.1.1 / P16256 Pantothenate:Na+ symporter, PanF from Escherichia coli (see 5 papers)
panF / GB|AAC76290.2 sodium/pantothenate symporter from Escherichia coli K12 (see 5 papers)
    27% identity, 88% coverage of query (116 bits)

TC 2.A.21.7.4 / D5AU53 Acetate uptake permease, ActP1; also takes up tellurite from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
    25% identity, 86% coverage of query (102 bits)

PS417_08865 large component of pyruvate transporter (actP-like) from Pseudomonas simiae WCS417
    23% identity, 98% coverage of query (94.0 bits)

Pf1N1B4_3673 large component of pyruvate transporter (actP-like) from Pseudomonas fluorescens FW300-N1B4
    23% identity, 88% coverage of query (93.6 bits)

Pf6N2E2_5849 large component of pyruvate transporter (actP-like) from Pseudomonas fluorescens FW300-N2E2
    23% identity, 88% coverage of query (92.0 bits)

TC 2.A.21.7.1 / O50471 Phenylacetate permease, Ppa from Pseudomonas putida (see paper)
    24% identity, 86% coverage of query (89.7 bits)

SGLT_VIBPH / P96169 Sodium/glucose cotransporter; Na(+)/glucose symporter from Vibrio parahaemolyticus (see paper)
TC 2.A.21.3.2 / P96169 Glucose or galactose:Na+ symporter, SglS or SglT of 543 aas and 14 TMSs (Turk et al. 2006). The 3.0 Å structure is known (Faham et al., 2008). Sodium exit causes a reorientation of transmembrane helix 1 that opens an inner gate required for substrate exit (Watanabe et al., 2010). The involvement of aromatic residue pi interactions, especially with Na+ binding, has been examined (see 3 papers)
AAF80602.1 Na+/galactose cotransporter SGLT from Vibrio parahaemolyticus (see paper)
    26% identity, 87% coverage of query (88.6 bits)

TC 2.A.21.7.5 / D5APM1 Acetate permease ActP-2/ActP2/ActP3 from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
    23% identity, 87% coverage of query (87.8 bits)

AZOBR_RS02940 large component of pyruvate transporter (actP-like) from Azospirillum brasilense Sp245
    24% identity, 98% coverage of query (87.0 bits)

MCTC_CORGL / Q8NS49 Monocarboxylic acid transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
TC 2.A.21.7.3 / Q8NS49 Pyruvate/acetate/propionate: H+ symporter, MctC (DhlC; cg0953) from Corynebacterium glutamicum (see paper)
    25% identity, 89% coverage of query (81.6 bits)

BT0355 L-arabinose/L-arabinobiose symporter from Bacteroides thetaiotaomicron VPI-5482
    25% identity, 87% coverage of query (76.6 bits)

TC 2.A.21.3.14 / P53794 Sodium/myo-inositol cotransporter (Na(+)/myo-inositol cotransporter) (Sodium/myo-inositol transporter 1) (SMIT1) (Solute carrier family 5 member 3) from Homo sapiens (see 3 papers)
    25% identity, 87% coverage of query (75.5 bits)

SC5A3_HUMAN / P53794 Sodium/myo-inositol cotransporter; Na(+)/myo-inositol cotransporter; Sodium/myo-inositol transporter 1; SMIT1; Solute carrier family 5 member 3 from Homo sapiens (Human) (see 4 papers)
    25% identity, 87% coverage of query (75.5 bits)

SC5A4_RAT / D3ZIS0 Solute carrier family 5 member 4 from Rattus norvegicus (Rat) (see paper)
    23% identity, 89% coverage of query (74.7 bits)

SC5A3_CANLF / P31637 Sodium/myo-inositol cotransporter; Na(+)/myo-inositol cotransporter; Sodium/myo-inositol transporter 1; SMIT1; Solute carrier family 5 member 3 from Canis lupus familiaris (Dog) (Canis familiaris) (see 2 papers)
TC 2.A.21.3.5 / P31637 Myoinositol:Na+ symporter, SMIT1 from Canis familiaris (Dog) (see paper)
    25% identity, 86% coverage of query (73.2 bits)

Echvi_1871 transporter for D-glucose/D-galactose/D-xylose/methyl-beta-D-galactopyranoside from Echinicola vietnamensis KMM 6221, DSM 17526
    24% identity, 87% coverage of query (72.8 bits)

SC5A3_MOUSE / Q9JKZ2 Sodium/myo-inositol cotransporter; Na(+)/myo-inositol cotransporter; Sodium/myo-inositol transporter 1; SMIT1; Solute carrier family 5 member 3 from Mus musculus (Mouse) (see 3 papers)
    24% identity, 87% coverage of query (72.0 bits)

Echvi_1880 L-arabinose symporter from Echinicola vietnamensis KMM 6221, DSM 17526
    26% identity, 98% coverage of query (71.2 bits)

S5A4A_MOUSE / Q9ET37 Solute carrier family 5 member 4A; SGLT3-a from Mus musculus (Mouse) (see 2 papers)
    23% identity, 87% coverage of query (69.7 bits)

SC5A7_MOUSE / Q8BGY9 High affinity choline transporter 1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Mus musculus (Mouse) (see 3 papers)
    24% identity, 88% coverage of query (68.6 bits)

SC5A8_XENLA / Q7SYH5 Sodium-coupled monocarboxylate transporter 1; Electrogenic sodium monocarboxylate cotransporter; xSMCTe; Sodium solute transporter Vito; Solute carrier family 5 member 8 from Xenopus laevis (African clawed frog) (see paper)
    23% identity, 82% coverage of query (68.2 bits)

SC5A4_HUMAN / Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see 4 papers)
TC 2.A.21.3.18 / Q9NY91 Low affinity sodium-glucose cotransporter (Sodium/glucose cotransporter 3) (Na+/glucose cotransporter 3) (Solute carrier family 5 member 4) from Homo sapiens (see paper)
    23% identity, 86% coverage of query (68.2 bits)

SC5A7_TORMA / Q8UWF0 High-affinity choline transporter 1 from Torpedo marmorata (Marbled electric ray) (see paper)
    23% identity, 81% coverage of query (68.2 bits)

SC5A7_RAT / Q9JMD7 High affinity choline transporter 1; rCHT1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.21.8.1 / Q9JMD7 High affinity neuronal choline:Na+ symporter, CHT1 (chloride-dependent) from Rattus norvegicus (Rat) (see paper)
Slc5a7 / RF|NP_445973.1 high affinity choline transporter 1 from Rattus norvegicus
    24% identity, 87% coverage of query (66.2 bits)

3dh4A / P96169 Crystal structure of sodium/sugar symporter with bound galactose from vibrio parahaemolyticus (see paper)
    25% identity, 74% coverage of query (65.9 bits)

8j74A / Q9GZV3 Human high-affinity choline transporter cht1 in the hc-3-bound outward-facing open conformation, dimeric state (see paper)
    26% identity, 87% coverage of query (63.9 bits)

SC5A8_HUMAN / Q8N695 Sodium-coupled monocarboxylate transporter 1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter; Solute carrier family 5 member 8 from Homo sapiens (Human) (see 16 papers)
TC 2.A.21.5.3 / Q8N695 Na+-dependent short chain fatty acid transporter SLC5A8 (tumor suppressor gene product, down-regulated in colon cancer) (substrates: lactate, pyruvate, acetate, propionate, butyrate (Km ≈1 mM)) [propionate:Na+ = 1:3] (Miyauchi et al., 2004). Pyroglutamate (5-oxoproline) is also transported in a Na+- coupled mechanism (Miyauchi et al., 2010). SMCT1 and SMCT2 may transport monocarboxylate drugs (e.g. nicotinate and its derivatives) across the intestinal brush boarder membrane (see 14 papers)
    24% identity, 82% coverage of query (63.9 bits)

SC5A7_HUMAN / Q9GZV3 High affinity choline transporter 1; hCHT1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Homo sapiens (Human) (see 10 papers)
TC 2.A.21.8.2 / Q9GZV3 High affinity choline transporter 1 (Hemicholinium-3-sensitive choline transporter) (CHT1) (Solute carrier family 5 member 7) from Homo sapiens (see 5 papers)
    25% identity, 87% coverage of query (63.5 bits)

7wmvA / P13866 Structure of human sglt1-map17 complex bound with lx2761 (see paper)
    24% identity, 85% coverage of query (63.2 bits)

SC5A1_HUMAN / P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 13 papers)
TC 2.A.21.3.1 / P13866 Glucose or galactose:Na+ symporter, SGLT1 (galactose > glucose > fucose). Cotransports water against an osmotic gradient (Naftalin, 2008). TMS IV of the high-affinity sodium-glucose cotransporter participates in sugar binding from Homo sapiens (Human) (see 9 papers)
SLC5A1 / GI|56202462 sodium/glucose cotransporter 1 from Homo sapiens (see paper)
    24% identity, 85% coverage of query (63.2 bits)

YidK / b3679 putative transporter YidK from Escherichia coli K-12 substr. MG1655 (see 5 papers)
    24% identity, 81% coverage of query (63.2 bits)

SC5A8_DANRE / Q3ZMH1 Sodium-coupled monocarboxylate transporter 1; Electrogenic sodium monocarboxylate cotransporter; zSMCTe; Sodium solute symporter family 5 member 8 protein; Solute carrier family 5 member 8 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
TC 2.A.21.5.5 / Q3ZMH1 The high affinity (Km (lactate) = 0.2mM) electrogenic Na+ monocarboxylate (lactate, pyruvate, butyrate, nicotinate) transporter, SMCTe from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
slc5a8 / RF|NP_001030141.1 sodium-coupled monocarboxylate transporter 1 from Danio rerio (see paper)
    23% identity, 85% coverage of query (62.8 bits)

SC5A7_CAEEL / O02228 High-affinity choline transporter 1 from Caenorhabditis elegans (see paper)
    23% identity, 72% coverage of query (59.7 bits)

SC5A1_MOUSE / Q8C3K6 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Mus musculus (Mouse) (see 2 papers)
    23% identity, 86% coverage of query (59.7 bits)

SC5A8_MOUSE / Q8BYF6 Sodium-coupled monocarboxylate transporter 1; Electrogenic sodium monocarboxylate cotransporter; Solute carrier family 5 member 8 from Mus musculus (Mouse) (see 8 papers)
    23% identity, 82% coverage of query (59.7 bits)

S5A4B_MOUSE / Q91ZP4 Solute carrier family 5 member 4B; Low affinity sodium-glucose cotransporter; SGLT3-b from Mus musculus (Mouse) (see 4 papers)
    22% identity, 76% coverage of query (59.3 bits)

SC5A2_MOUSE / Q923I7 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Mus musculus (Mouse) (see paper)
    24% identity, 78% coverage of query (58.5 bits)

SC5A5_HUMAN / Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 12 papers)
TC 2.A.21.5.1 / Q92911 Sodium iodide symporter (NIS; also transports other monovalent anions including: ClO3-, SCN-, SeCN-, NO3-, Br-, BF4-, IO4- and BrO3-). It mediates electroneutral active transport of the environmental pollutant perchlorate (Dohan et al., 2007). Five beta-OH group-containing residues (Thr-351, Ser-353, Thr-354, Ser-356, and Thr-357) and Asn-360, all of which putatively face the same side of the helix in TMS IX, plus Asp-369, located in the membrane/cytosol interface, play key roles in NIS function and seem to be involved in Na+ binding/translocation (De la Vieja et al. 2007). Thr-354 is essential for iodide uptake (Tatsumi et al., 2010). The stoichiometry is Na+:I-= 2:1. The G39R mutant (congenital) is inactive. G93 is a pivot for the inwardly to outwardly conformational change from Homo sapiens (Human) (see 8 papers)
    22% identity, 74% coverage of query (58.5 bits)

SC5A2_RAT / P53792 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Rattus norvegicus (Rat) (see paper)
    24% identity, 78% coverage of query (58.5 bits)

NANT_ALIF1 / Q5E733 N-acetylneuraminate transporter from Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri) (see paper)
    21% identity, 87% coverage of query (58.2 bits)

CA265_RS06130 D-galactose transporter from Pedobacter sp. GW460-11-11-14-LB5
    23% identity, 78% coverage of query (55.8 bits)

TC 2.A.21.3.12 / A3UWQ9 The alginate oligosaccharide uptake porter, ToaB from Vibrio splendidus 12B01
    24% identity, 77% coverage of query (55.5 bits)

SC5A2_RABIT / P26430 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Oryctolagus cuniculus (Rabbit) (see paper)
TC 2.A.21.3.3 / P26430 Nucleoside or glucose(?):Na+ symporter from Oryctolagus cuniculus (Rabbit) (see paper)
    24% identity, 78% coverage of query (55.5 bits)

7slaA / P13866 Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
    22% identity, 74% coverage of query (52.8 bits)

7sl8A Cryoem structure of sglt1 at 3.4 a resolution
    22% identity, 74% coverage of query (52.4 bits)

7sl9A / Q8N695 Cryoem structure of smct1 (see paper)
    24% identity, 80% coverage of query (51.6 bits)

SC5A2_HUMAN / P31639 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Homo sapiens (Human) (see 4 papers)
TC 2.A.21.3.16 / P31639 Sodium/glucose cotransporter 2 (Na+/glucose cotransporter 2) (Low affinity sodium-glucose cotransporter) (Solute carrier family 5 member 2) of 672 aas and 14 TMSs from Homo sapiens (see 2 papers)
    24% identity, 76% coverage of query (50.8 bits)

8hdhA Structure of human sglt2-map17 complex with canagliflozin
    24% identity, 76% coverage of query (50.4 bits)

8hb0A Structure of human sglt2-map17 complex with ta1887
    24% identity, 76% coverage of query (50.4 bits)

8hg7A / P31639 Structure of human sglt2-map17 complex with sotagliflozin (see paper)
    24% identity, 76% coverage of query (50.4 bits)

8hezA Structure of human sglt2-map17 complex with dapagliflozin
    24% identity, 76% coverage of query (50.4 bits)

7vsiA Structure of human sglt2-map17 complex bound with empagliflozin
    24% identity, 76% coverage of query (50.4 bits)

8j77A Human high-affinity choline transporter cht1 in the choline-bound inward-facing occluded conformation
    25% identity, 81% coverage of query (48.1 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory