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Searching for up to 100 curated homologs for GFF4325 FitnessBrowser__WCS417:GFF4325 (419 a.a.)

Found high-coverage hits (≥70%) to 21 curated proteins.

Removed hits that are identical to the query, leaving 20

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO353_03400 D-mannose isomerase (EC 5.3.1.7) from Pseudomonas fluorescens FW300-N2E3
    75% identity, 99% coverage of query (628 bits)

AO356_05200 D-mannose isomerase (EC 5.3.1.7) from Pseudomonas fluorescens FW300-N2C3
    75% identity, 96% coverage of query (583 bits)

Pf1N1B4_597 D-mannose isomerase (EC 5.3.1.7) from Pseudomonas fluorescens FW300-N1B4
    76% identity, 96% coverage of query (573 bits)

MANI_PSEC1 / A0A0P9JFY5 D-mannose isomerase; PsMIaseA; EC 5.3.1.7 from Pseudomonas cannabina pv. alisalensis (strain ATCC BAA-566 / CFBP 6866 / ICMP 15200 / BS91) (see paper)
    70% identity, 96% coverage of query (540 bits)

SquS / b3880 sulfoquinovose isomerase (EC 5.3.1.31; EC 5.3.1.7) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
yihS / P32140 sulfoquinovose isomerase (EC 5.3.1.31; EC 5.3.1.7) from Escherichia coli (strain K12) (see 6 papers)
SQUS_ECOLI / P32140 Sulfoquinovose isomerase; SQ isomerase; Sulfoquinovose-sulfofructose isomerase; SQ-SF isomerase; EC 5.3.1.31 from Escherichia coli (strain K12) (see 3 papers)
yihS / RF|NP_418316 uncharacterized sugar isomerase yihS from Escherichia coli K12 (see paper)
    47% identity, 96% coverage of query (379 bits)

SQUS_SALTY / Q8ZKT7 Sulfoquinovose isomerase; SQ isomerase; Sulfoquinovose-sulfofructose isomerase; SQ-SF isomerase; EC 5.3.1.31 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
    46% identity, 96% coverage of query (368 bits)

7ag4D / Q8ZKT7 Crystal structure of active site mutant of sq isomerase (yihs-h248a) from salmonella enterica in complex with sulfofructose (sf) (see paper)
    45% identity, 96% coverage of query (365 bits)

2zblA Functional annotation of salmonella enterica yihs-encoded protein
    45% identity, 96% coverage of query (364 bits)

MANI_THEFU / A0A077LPS9 D-mannose isomerase; EC 5.3.1.7 from Thermobifida fusca (Thermomonospora fusca)
    49% identity, 92% coverage of query (324 bits)

PGA1_c16670 D-mannose isomerase (EC 5.3.1.7) from Phaeobacter inhibens BS107
    39% identity, 92% coverage of query (228 bits)

BPHYT_RS02050 D-mannose isomerase (EC 5.3.1.7) from Burkholderia phytofirmans PsJN
    33% identity, 86% coverage of query (165 bits)

MANI_MARM1 / F2JVT6 D-mannose isomerase; MI; EC 5.3.1.7 from Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1) (see paper)
    30% identity, 86% coverage of query (149 bits)

Sama_0560 D-mannose isomerase (EC 5.3.1.7) from Shewanella amazonensis SB2B
    31% identity, 81% coverage of query (139 bits)

Ac3H11_2078 D-mannose isomerase (EC 5.3.1.7) from Acidovorax sp. GW101-3H11
    31% identity, 75% coverage of query (139 bits)

Build an alignment

Build an alignment for GFF4325 and 14 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

E0RU15 cellobiose epimerase (EC 5.1.3.11) from Spirochaeta thermophila (see 2 papers)
    25% identity, 72% coverage of query (63.5 bits)

G0GBL0 cellobiose epimerase (EC 5.1.3.11) from Spirochaeta thermophila (see paper)
    24% identity, 72% coverage of query (62.8 bits)

C6VW66 cellobiose epimerase (EC 5.1.3.11) from Dyadobacter fermentans (see 2 papers)
    23% identity, 80% coverage of query (57.4 bits)

A5FA14 cellobiose epimerase (EC 5.1.3.11) from Flavobacterium johnsoniae (see 2 papers)
    21% identity, 79% coverage of query (54.3 bits)

D0MF61 cellobiose epimerase (EC 5.1.3.11) from Rhodothermus marinus (see paper)
    25% identity, 87% coverage of query (51.2 bits)

F5ITJ6 cellobiose epimerase (EC 5.1.3.11) from Dysgonomonas gadei (see paper)
    21% identity, 78% coverage of query (50.4 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory