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Searching for up to 100 curated homologs for H281DRAFT_02563 FitnessBrowser__Burk376:H281DRAFT_02563 (265 a.a.)

Found high-coverage hits (≥70%) to 35 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

TC 3.A.1.3.12 / P73544 BgtB aka GLNH aka SLL1270, component of Arginine/lysine/histidine/glutamine porter (see paper)
    35% identity, 80% coverage of query (133 bits)

TC 3.A.1.3.17 / Q8YSA2 Basic amino acid uptake transporter, BgtAB (see paper)
    37% identity, 87% coverage of query (132 bits)

GltK / b0653 glutamate/aspartate ABC transporter membrane subunit GltK (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
GltK / P0AER5 glutamate/aspartate ABC transporter membrane subunit GltK (EC 7.4.2.1) from Escherichia coli (strain K12) (see 3 papers)
GLTK_ECOLI / P0AER5 Glutamate/aspartate import permease protein GltK from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.3.4 / P0AER5 Glutamate/aspartate transport system permease protein GltK aka B0653, component of Glutamate/aspartate porter from Escherichia coli (see 4 papers)
    37% identity, 83% coverage of query (125 bits)

4ymtC / Q8RCC3 Crystal structure of an amino acid abc transporter complex with arginines (see paper)
    33% identity, 82% coverage of query (124 bits)

4ymwC Crystal structure of an amino acid abc transporter with histidines
    33% identity, 82% coverage of query (124 bits)

ARTQ_BACSU / P54536 Arginine transport system permease protein ArtQ from Bacillus subtilis (strain 168) (see paper)
    34% identity, 82% coverage of query (120 bits)

TcyL / b1918 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
tcyL / P0AFT2 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli (strain K12) (see 3 papers)
TCYL_ECOLI / P0AFT2 L-cystine transport system permease protein TcyL from Escherichia coli (strain K12) (see 5 papers)
TC 3.A.1.3.10 / P0AFT2 Inner membrane amino-acid ABC transporter permease protein YECS CysY aka B1918, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 4 papers)
    34% identity, 82% coverage of query (114 bits)

GLNM_BACSU / O34671 Probable glutamine ABC transporter permease protein GlnM from Bacillus subtilis (strain 168) (see paper)
    33% identity, 77% coverage of query (113 bits)

TC 3.A.1.3.22 / Q9I404 Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    34% identity, 82% coverage of query (107 bits)

YXEN_BACSU / P54953 Probable amino-acid permease protein YxeN from Bacillus subtilis (strain 168) (see paper)
    31% identity, 78% coverage of query (106 bits)

AO353_16280 ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 1 from Pseudomonas fluorescens FW300-N2E3
    33% identity, 82% coverage of query (105 bits)

PfGW456L13_4772 ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 2 from Pseudomonas fluorescens GW456-L13
    33% identity, 82% coverage of query (103 bits)

TC 3.A.1.3.23 / Q9HU29 Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    34% identity, 82% coverage of query (103 bits)

SMc02120 ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, permease component 2 from Sinorhizobium meliloti 1021
    31% identity, 77% coverage of query (103 bits)

TC 3.A.1.3.19 / Q88NY4 PP1069, component of Acidic amino acid uptake porter, AatJMQP from Pseudomonas putida (strain KT2440) (see paper)
    34% identity, 82% coverage of query (100 bits)

GlnP / b0810 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
GlnP / P0AEQ6 L-glutamine ABC transporter membrane subunit (EC 7.4.2.1) from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.3.2 / P0AEQ6 Glutamine transport system permease protein GlnP aka B0810, component of Three component ABC L-glutamine porter. The basal ATPase activity (ATP hydrolysis in the absence of substrate) is mainly caused by the docking of the closed-unliganded state of GlnH onto the transporter domain of GlnPQ. Unlike glutamine, arginine binds both GlnH domains, but does not trigger their closing. Comparison of the ATPase activity in nanodiscs with glutamine transport in proteoliposomes suggested that the stoichiometry of ATP per substrate is close to two from Escherichia coli (see 5 papers)
    35% identity, 82% coverage of query (100 bits)

Build an alignment

Build an alignment for H281DRAFT_02563 and 16 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

BPHYT_RS07680 ABC transporter for L-Arginine, permease component 1 from Burkholderia phytofirmans PsJN
    29% identity, 85% coverage of query (98.6 bits)

TC 3.A.1.3.6 / P35113 NocM aka ATU6025 aka AGR_PTI_65, component of Nopaline porter from Agrobacterium tumefaciens (see 3 papers)
    31% identity, 83% coverage of query (98.6 bits)

TC 3.A.1.3.8 / Q52814 AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) from Rhizobium leguminosarum (biovar viciae) (see 2 papers)
aapM / CAA57935.1 general amino acid ABC type transporter from Rhizobium leguminosarum (see paper)
    30% identity, 80% coverage of query (98.2 bits)

TCYB_BACSU / P42200 L-cystine transport system permease protein TcyB from Bacillus subtilis (strain 168) (see paper)
TC 3.A.1.3.14 / P42200 TcyB (YckJ), component of Uptake system for L-cystine from Bacillus subtilis (see 4 papers)
    29% identity, 84% coverage of query (96.7 bits)

ARGU_CORGL / Q8NQU3 Arginine transport system permease protein ArgU from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
    32% identity, 82% coverage of query (94.7 bits)

TC 3.A.1.3.16 / A1VZQ3 PEP1B, component of Uptake system for glutamate and aspartate from Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
    28% identity, 74% coverage of query (94.7 bits)

GLUD_CORGL / P48245 Glutamate transport system permease protein GluD; Glutamate uptake system protein GluD from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
TC 3.A.1.3.9 / P48245 GluD aka CGL1953, component of Glutamate porter from Corynebacterium glutamicum (Brevibacterium flavum) (see 2 papers)
    30% identity, 89% coverage of query (94.4 bits)

AO353_21720 ABC transporter for D-glucosamine, permease component 2 from Pseudomonas fluorescens FW300-N2E3
    27% identity, 76% coverage of query (90.9 bits)

YhdY / b3270 putative ABC transporter membrane subunit YhdY from Escherichia coli K-12 substr. MG1655 (see 5 papers)
    31% identity, 80% coverage of query (90.5 bits)

Ac3H11_2554 ABC transporter for L-Histidine, permease component 1 from Acidovorax sp. GW101-3H11
    29% identity, 82% coverage of query (90.1 bits)

Pf6N2E2_5404 ABC transporter for D-Alanine, permease component 1 from Pseudomonas fluorescens FW300-N2E2
    31% identity, 76% coverage of query (89.7 bits)

AO356_00470 ABC transporter for D-Glucosamine, permease component 1 from Pseudomonas fluorescens FW300-N2C3
Pf6N2E2_2051 ABC transporter for D-Glucosamine Hydrochloride, permease component 1 from Pseudomonas fluorescens FW300-N2E2
    27% identity, 76% coverage of query (87.8 bits)

PP_3594 L-lysine and D-lysine ABC transporter, permease component 1 from Pseudomonas putida KT2440
    31% identity, 79% coverage of query (87.4 bits)

Pf1N1B4_3432 ABC transporter for L-Arginine and L-Citrulline, permease component 1 from Pseudomonas fluorescens FW300-N1B4
    31% identity, 74% coverage of query (86.7 bits)

Pf6N2E2_5661 L-Arginine ABC transporter, permease protein AotQ from Pseudomonas fluorescens FW300-N2E2
    31% identity, 74% coverage of query (85.9 bits)

AO356_18705 L-Arginine ABC transporter, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    31% identity, 74% coverage of query (85.5 bits)

PS417_21740 ABC transporter for L-Arginine, permease component 1 from Pseudomonas simiae WCS417
    31% identity, 74% coverage of query (85.1 bits)

AO356_05505 ABC transporter for L-Lysine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    28% identity, 79% coverage of query (84.3 bits)

AO356_09910 ABC transporter for L-Lysine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    31% identity, 82% coverage of query (84.0 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory