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Searching for up to 100 curated homologs for H281DRAFT_02672 FitnessBrowser__Burk376:H281DRAFT_02672 (264 a.a.)

Found high-coverage hits (≥70%) to 70 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    34% identity, 84% coverage of query (115 bits)

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    31% identity, 95% coverage of query (113 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    34% identity, 84% coverage of query (113 bits)

Build an alignment

Build an alignment for H281DRAFT_02672 and 3 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    29% identity, 94% coverage of query (103 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    32% identity, 89% coverage of query (103 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    29% identity, 94% coverage of query (103 bits)

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    29% identity, 87% coverage of query (102 bits)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
    29% identity, 97% coverage of query (99.8 bits)

6eb3C Structural and enzymatic characterization of an esterase from a metagenomic library
    29% identity, 97% coverage of query (99.8 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    29% identity, 94% coverage of query (99.0 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    29% identity, 94% coverage of query (99.0 bits)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    29% identity, 94% coverage of query (98.6 bits)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
    29% identity, 97% coverage of query (98.6 bits)

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    31% identity, 84% coverage of query (95.9 bits)

Q8KT44 carboxylesterase (EC 3.1.1.1) from Lacticaseibacillus casei (see paper)
    27% identity, 95% coverage of query (95.5 bits)

O73957 carboxylesterase (EC 3.1.1.1) from Sulfolobus acidocaldarius (see paper)
    27% identity, 94% coverage of query (95.1 bits)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    30% identity, 87% coverage of query (91.7 bits)

BPOC_MYCTU / P9WNH1 Putative non-heme bromoperoxidase BpoC from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    29% identity, 95% coverage of query (88.2 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    26% identity, 90% coverage of query (82.0 bits)

TGND_ACIAD / Q6F9F4 (E)-2-((N-methylformamido)methylene)succinate hydrolase; MFMS hydrolase; EC 3.5.1.- from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
    23% identity, 91% coverage of query (80.9 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    25% identity, 90% coverage of query (75.5 bits)

YVAM_BACSU / O32234 AB hydrolase superfamily protein YvaM; EC 3.-.-.- from Bacillus subtilis (strain 168) (see paper)
    25% identity, 75% coverage of query (71.2 bits)

THAQ_BURTA / Q2T4P1 Polyketide synthase ThaQ from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264) (see paper)
    29% identity, 97% coverage of query (69.3 bits)

5frdA / O28735 Structure of a thermophilic esterase (see paper)
    24% identity, 100% coverage of query (69.3 bits)

estRB8 / CAE54381.1 carboxylesterase, partial from Oleispira antarctica (see paper)
estRB8 / CAE54384.1 carboxylesterase from Oleispira antarctica (see paper)
    24% identity, 93% coverage of query (66.6 bits)

tesD / Q83VZ6 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (EC 3.7.1.17) from Comamonas testosteroni (see 3 papers)
    25% identity, 94% coverage of query (65.9 bits)

7c4dA / A0A2S1GUX0 Marine microorganism esterase (see paper)
    24% identity, 92% coverage of query (65.5 bits)

Q84II3 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13) from Janthinobacterium sp. J3 (see paper)
    26% identity, 98% coverage of query (64.7 bits)

carC / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)-hexa-2,4dienoate hydrolase monomer (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
CARC_PSERE / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase; HOPDA; EC 3.7.1.13 from Pseudomonas resinovorans (see 3 papers)
Q9AQM4 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
carC / BAC41548.1 meta cleavage compound hydrolase from Pseudomonas resinovorans (see 9 papers)
    26% identity, 99% coverage of query (64.3 bits)

A0A0H2WW38 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (EC 4.2.99.20) from Staphylococcus aureus (see paper)
    25% identity, 91% coverage of query (62.0 bits)

6i8wB / Q9KJG6 Crystal structure of a membrane phospholipase a, a novel bacterial virulence factor (see paper)
    27% identity, 93% coverage of query (60.5 bits)

Q9KJG6 triacylglycerol lipase (EC 3.1.1.3) from Pseudomonas aeruginosa (see paper)
    27% identity, 93% coverage of query (60.1 bits)

P29715 bromide peroxidase (EC 1.11.1.18) from Kitasatospora aureofaciens (see 2 papers)
bpoA2 / GI|150457 non-heme bromoperoxidase BPO-A2; EC 1.11.1.- from Streptomyces aureofaciens (see 4 papers)
    26% identity, 86% coverage of query (60.1 bits)

ethA2 / O53622 monooxygenase EthA2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    28% identity, 97% coverage of query (60.1 bits)

bphD / BAA25612.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus erythropolis (see paper)
    24% identity, 94% coverage of query (59.7 bits)

5h3hB / K0ACL0 Esterase (eaest) from exiguobacterium antarcticum (see paper)
    23% identity, 97% coverage of query (59.3 bits)

ABHDB_BOVIN / Q3SZ73 sn-1-specific diacylglycerol lipase ABHD11; Alpha/beta hydrolase domain-containing protein 11; Abhydrolase domain-containing protein 11; EC 3.1.1.116 from Bos taurus (Bovine) (see paper)
    25% identity, 89% coverage of query (58.5 bits)

cpoT / GB|AAB86626.1 non-heme chloroperoxidase from Streptomyces aureofaciens (see paper)
    26% identity, 86% coverage of query (57.8 bits)

4lxhA Crystal structure of the s105a mutant of a carbon-carbon bond hydrolase, dxnb2 from sphingomonas wittichii rw1, in complex with 3- cl hopda (see paper)
    27% identity, 98% coverage of query (57.4 bits)

PFMAB_PESFW / W3XA95 Proline iminopeptidase PfmaB; PIP; Conidial pigment biosynthesis cluster protein B; EC 3.4.11.5 from Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) (see 2 papers)
    27% identity, 89% coverage of query (57.4 bits)

4lyeA Crystal structure of the s105a mutant of a c-c hydrolase, dxnb2 from sphingomonas wittichii rw1, in complex with substrate hopda
    27% identity, 98% coverage of query (57.4 bits)

4lxiA Crystal structure of the s105a mutant of a carbon-carbon bond hydrolase, dxnb2 from sphingomonas wittichii rw1, in complex with 5, 8-dif hopda
    27% identity, 98% coverage of query (57.4 bits)

1hl7A / Q8GJP7 Gamma lactamase from an aureobacterium species in complex with 3a,4,7, 7a-tetrahydro-benzo [1,3] dioxol-2-one (see paper)
    28% identity, 87% coverage of query (56.6 bits)

hbpD / O06648 2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoate hydrolase [multifunctional] (EC 3.7.1.8) from Pseudomonas nitroreducens (see 2 papers)
    22% identity, 94% coverage of query (56.6 bits)

cpoF / PDB|1A8S non-heme chloroperoxidase; EC 1.11.1.10 from Pseudomonas fluorescens (see 2 papers)
    27% identity, 90% coverage of query (56.6 bits)

5z7wB / A0A0M4AV81 Crystal structure of striga hermonthica htl1 (shhtl1) (see paper)
    25% identity, 79% coverage of query (55.8 bits)

RSBQ_BACSU / O07015 Sigma factor SigB regulation protein RsbQ from Bacillus subtilis (strain 168) (see 2 papers)
    24% identity, 84% coverage of query (55.5 bits)

E3SVS0 amidase (EC 3.5.1.4) from Microbacterium hydrocarbonoxydans (see paper)
    26% identity, 87% coverage of query (54.7 bits)

7a7gA Soluble epoxide hydrolase in complex with tk90
    24% identity, 93% coverage of query (54.7 bits)

phaZ / Q8KQ22 intracellular poly(3-hydroxyoctanoate) depolymerase (EC 3.1.1.76) from Pseudomonas putida (see 6 papers)
    24% identity, 91% coverage of query (53.5 bits)

5fp0A Ligand complex structure of soluble epoxide hydrolase
    23% identity, 93% coverage of query (53.5 bits)

lnmJ / Q8GGP2 leinamycin polyketide synthase LnmJ from Streptomyces atroolivaceus (see 5 papers)
    25% identity, 91% coverage of query (53.1 bits)

PHAZ_PSEOL / P26495 Poly(3-hydroxyalkanoate) depolymerase; PHA depolymerase; ORF2; PHB depolymerase; EC 3.1.1.- from Pseudomonas oleovorans (see paper)
phaB / AAA25933.1 PHA-depolymerase from Pseudomonas oleovorans (see paper)
    24% identity, 91% coverage of query (52.8 bits)

5aljA / P34913 Ligand complex structure of soluble epoxide hydrolase (see paper)
    24% identity, 93% coverage of query (52.8 bits)

cpoL / PDB|1A88_A non-heme chloroperoxidase; EC 1.11.1.10 from Streptomyces lividans (see 2 papers)
cpoL / AAA18642.1 chloroperoxidase from Streptomyces lividans (see paper)
    27% identity, 82% coverage of query (52.8 bits)

7p4kA Soluble epoxide hydrolase in complex with fl217
    24% identity, 93% coverage of query (52.8 bits)

BioB / b3412 pimeloyl-acyl carrier protein methyl ester esterase (EC 3.1.1.85; EC 3.1.1.1) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
bioH / P13001 pimeloyl-acyl carrier protein methyl ester esterase (EC 3.1.1.85; EC 3.1.1.1) from Escherichia coli (strain K12) (see 8 papers)
BIOH_ECOLI / P13001 Pimeloyl-[acyl-carrier protein] methyl ester esterase; Biotin synthesis protein BioH; Carboxylesterase BioH; EC 3.1.1.85 from Escherichia coli (strain K12) (see 4 papers)
P13001 carboxylesterase (EC 3.1.1.1); pimelyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Escherichia coli (see paper)
    25% identity, 87% coverage of query (52.0 bits)

1m33A / P13001 Crystal structure of bioh at 1.7 a (see paper)
    25% identity, 87% coverage of query (51.6 bits)

4etwA Structure of the enzyme-acp substrate gatekeeper complex required for biotin synthesis
    24% identity, 87% coverage of query (50.8 bits)

3heaA The l29p/l124i mutation of pseudomonas fluorescens esterase
    23% identity, 85% coverage of query (50.1 bits)

3hi4A Switching catalysis from hydrolysis to perhydrolysis in p. Fluorescens esterase
    23% identity, 85% coverage of query (50.1 bits)

ESTE_PSEFL / P22862 Arylesterase; Aryl-ester hydrolase; Carboxylic acid perhydrolase; PFE; Putative bromoperoxidase; EC 3.1.1.2; EC 1.-.-.- from Pseudomonas fluorescens (see 8 papers)
GI|951089 arylesterase; EC 1.-.-.-; EC 3.1.1.2 from Pseudomonas fluorescens (see 3 papers)
AAB60168.1 esterase from Pseudomonas fluorescens (see 2 papers)
    23% identity, 85% coverage of query (50.1 bits)

3ia2A Pseudomonas fluorescens esterase complexed to the r-enantiomer of a sulfonate transition state analog
    23% identity, 85% coverage of query (50.1 bits)

aamsh1 / Q988D4 2-(acetamidomethylene)succinate hydrolase monomer (EC 3.5.1.29) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (see 7 papers)
AAMHY_RHILO / Q988D4 2-(acetamidomethylene)succinate hydrolase; alpha-(N-acetylaminomethylene)succinic acid amidohydrolase; AAMS amidohydrolase; EC 3.5.1.29 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
Q988D4 2-(acetamidomethylene)succinate hydrolase (EC 3.5.1.29) from Mesorhizobium loti (see 2 papers)
    24% identity, 88% coverage of query (48.9 bits)

8pi1B / P22862 Bicyclic incypro pseudomonas fluorescens esterase (see paper)
    23% identity, 85% coverage of query (48.1 bits)

ABHDB_HUMAN / Q8NFV4 sn-1-specific diacylglycerol lipase ABHD11; Alpha/beta hydrolase domain-containing protein 11; Abhydrolase domain-containing protein 11; Williams-Beuren syndrome chromosomal region 21 protein; EC 3.1.1.116 from Homo sapiens (Human) (see 3 papers)
    26% identity, 79% coverage of query (47.8 bits)

2rhwA Crystal structure of the s112a mutant of a c-c hydrolase, bphd from burkholderia xenovorans lb400, in complex with 3,10-di-fluoro hopda
    22% identity, 98% coverage of query (46.6 bits)

2rhtA Crystal structure of the s112a mutant of a c-c hydrolase, bphd from burkholderia xenovorans lb400, in complex with 3-cl hopda
    22% identity, 98% coverage of query (46.6 bits)

2puhA Crystal structure of the s112a mutant of a c-c hydrolase, bphd from burkholderia xenovorans lb400, in complex with its substrate hopda
    22% identity, 98% coverage of query (46.6 bits)

MhpC / b0349 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
mhpC / P77044 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli (strain K12) (see 10 papers)
MHPC_ECOLI / P77044 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase; 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; EC 3.7.1.14 from Escherichia coli (strain K12) (see 4 papers)
P77044 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli (see 4 papers)
    22% identity, 94% coverage of query (46.2 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory