Searching for up to 100 curated homologs for H281DRAFT_05434 H281DRAFT_05434 Predicted amidohydrolase (298 a.a.)
Found high-coverage hits (≥70%) to 100 curated proteins.
You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.
3klcA / Q9UYV8 Crystal structure of hyperthermophilic nitrilase (see paper)
43% identity, 90% coverage of query (214 bits)
3klcB Crystal structure of hyperthermophilic nitrilase
43% identity, 90% coverage of query (214 bits)
NITR_PYRAB / Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see 2 papers)
43% identity, 90% coverage of query (212 bits)
F8AEP0 amidase (EC 3.5.1.4) from Pyrococcus yayanosii (see paper)
43% identity, 90% coverage of query (209 bits)
6ypaB / O58376 The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
41% identity, 92% coverage of query (207 bits)
7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide
42% identity, 90% coverage of query (207 bits)
YCU9_SCHPO / O59829 Probable nitrilase C965.09; EC 3.5.-.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 92% coverage of query (129 bits)
aguB / A0A1J6PBK3 N-carbamoylputrescine amidohydrolase (EC 3.5.1.53) from Campylobacter jejuni (see paper)
30% identity, 90% coverage of query (127 bits)
pydC / Q846U4 3-amino-isobutyrate synthase (EC 3.5.1.6) from Brevibacillus agri (see paper)
32% identity, 89% coverage of query (121 bits)
AGUB_SOLLC / Q9XGI9 N-carbamoylputrescine amidase; EC 3.5.1.53 from Solanum lycopersicum (Tomato) (Lycopersicon esculentum) (see paper)
32% identity, 86% coverage of query (115 bits)
At2g27450 / Q8VYF5 At2g27450-monomer (EC 3.5.1.53) from Arabidopsis thaliana (see paper)
NILP1_ARATH / Q8VYF5 N-carbamoylputrescine amidase; Nitrilase-like protein 1; EC 3.5.1.53 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 86% coverage of query (114 bits)
5h8iC / G7ITU5 Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with n-(dihydroxymethyl)putrescine (see paper)
31% identity, 86% coverage of query (114 bits)
5h8jB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with cadaverine
31% identity, 86% coverage of query (114 bits)
5h8lB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) c158s mutant in complex with putrescine
31% identity, 86% coverage of query (110 bits)
A0A088BHP3 aryl-acylamidase (EC 3.5.1.13); amidase (EC 3.5.1.4) from Streptomyces sp. (see paper)
35% identity, 90% coverage of query (109 bits)
mtnU / O31664 2-oxoglutaramate:glutamine aminotransferase (EC 3.5.1.3) from Bacillus subtilis (strain 168) (see 4 papers)
MTNU_BACSU / O31664 2-oxoglutaramate amidase; EC 3.5.1.111 from Bacillus subtilis (strain 168) (see 2 papers)
32% identity, 90% coverage of query (109 bits)
Build an alignment for H281DRAFT_05434 and 16 homologs with ≥ 30% identity
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ctu / Q5NHL7 citrullinase (EC 3.5.1.20) from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
CTU_FRATT / Q5NHL7 Citrullinase; Citrulline ureidase; CTU; EC 3.5.1.20 from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
Q5NHL7 citrullinase (EC 3.5.1.20) from Francisella tularensis subsp. tularensis (see paper)
29% identity, 93% coverage of query (108 bits)
Q44185 N-carbamoyl-D-amino acid hydrolase; D-N-alpha-carbamilase; EC 3.5.1.77 from Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)
30% identity, 81% coverage of query (108 bits)
NIT2_RAT / Q497B0 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Rattus norvegicus (Rat) (see 2 papers)
31% identity, 86% coverage of query (106 bits)
NIT2_HUMAN / Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 4 papers)
Q9NQR4 omega-amidase (EC 3.5.1.3) from Homo sapiens (see paper)
31% identity, 86% coverage of query (105 bits)
8hpcC / P60327 Crystal structure of c171a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-hydroxyphenylglycine
30% identity, 81% coverage of query (103 bits)
Nit2 / Q9JHW2 ω-amidase NIT2 monomer (EC 3.5.1.3) from Mus musculus (see 2 papers)
NIT2_MOUSE / Q9JHW2 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Mus musculus (Mouse) (see 3 papers)
30% identity, 86% coverage of query (103 bits)
NILP3_ARATH / Q8RUF8 Omega-amidase, chloroplastic; Nitrilase-like protein 3; EC 3.5.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q8RUF8 omega-amidase (EC 3.5.1.3) from Arabidopsis thaliana (see paper)
33% identity, 87% coverage of query (103 bits)
B8A0E5 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
34% identity, 87% coverage of query (103 bits)
DCAS_ENSAD / Q5S260 N-carbamoyl-D-amino acid hydrolase; D-carbamoylase; EC 3.5.1.77 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
Q5S260 N-carbamoyl-D-amino-acid hydrolase (EC 3.5.1.77) from Ensifer adhaerens (see paper)
29% identity, 81% coverage of query (103 bits)
B8A2V8 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
34% identity, 87% coverage of query (102 bits)
Q84FR7 N-carbamoyl-D-amino-acid hydrolase (EC 3.5.1.77) from Arthrobacter crystallopoietes (see paper)
34% identity, 85% coverage of query (102 bits)
1uf8A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-phenylalanine
30% identity, 81% coverage of query (102 bits)
1uf7A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-valine
30% identity, 81% coverage of query (102 bits)
1uf5A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-methionine
30% identity, 81% coverage of query (102 bits)
aguB / Q9I6J8 N-carbamoylputrascine amidohydrolase subunit (EC 3.5.1.53) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
30% identity, 91% coverage of query (100 bits)
SGR_6069 / B1W481 4-guanidinobutanamidase (EC 3.5.1.4) from Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) (see 2 papers)
35% identity, 85% coverage of query (100 bits)
A0A023XI92 N-carbamoyl-D-amino-acid hydrolase (EC 3.5.1.77) from Bradyrhizobium japonicum (see paper)
32% identity, 90% coverage of query (99.8 bits)
ramA / Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
RSAM_PSESP / Q75SP7 (R)-stereoselective amidase; EC 3.5.1.100 from Pseudomonas sp. (see paper)
Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
32% identity, 89% coverage of query (97.4 bits)
Q8PXI9 nitrilase (EC 3.5.5.1) from Methanosarcina mazei (see paper)
27% identity, 92% coverage of query (97.4 bits)
D7B8P3 nitrilase (EC 3.5.5.1) from Nocardiopsis dassonvillei ATCC 23218 (see paper)
34% identity, 80% coverage of query (96.3 bits)
B3IVI7 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (see 4 papers)
33% identity, 90% coverage of query (95.1 bits)
PfGW456L13_1170 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens GW456-L13
32% identity, 90% coverage of query (94.0 bits)
davA / Q88QV2 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
Q88QV2 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (see paper)
32% identity, 90% coverage of query (92.8 bits)
Pf1N1B4_2504 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N1B4
32% identity, 90% coverage of query (92.4 bits)
A0A0E3D8K7 nitrilase (EC 3.5.5.1); Aliphatic nitrilase (EC 3.5.5.7) from Fusarium proliferatum (see paper)
31% identity, 87% coverage of query (92.0 bits)
Pf6N2E2_4777 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2E2
32% identity, 91% coverage of query (90.9 bits)
AMIE_HELPY / O25067 Aliphatic amidase; Acylamide amidohydrolase; EC 3.5.1.4 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see 3 papers)
32% identity, 82% coverage of query (90.9 bits)
AMIE_BACSP / Q9L543 Aliphatic amidase; Acylamide amidohydrolase; EC 3.5.1.4 from Bacillus sp. (see paper)
32% identity, 78% coverage of query (90.5 bits)
4gylA / Q9L543 The e142l mutant of the amidase from geobacillus pallidus showing the result of michael addition of acrylamide at the active site cysteine (see paper)
32% identity, 78% coverage of query (90.5 bits)
S5M6X9 nitrilase (EC 3.5.5.1) from Paenarthrobacter aurescens (see paper)
29% identity, 83% coverage of query (89.0 bits)
AMIE_GEOSE / Q9RQ17 Aliphatic amidase; Acylamide amidohydrolase; Wide spectrum amidase; EC 3.5.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
32% identity, 87% coverage of query (89.0 bits)
A6T0X3 nitrilase (EC 3.5.5.1) from Janthinobacterium sp. Marseille (see paper)
30% identity, 84% coverage of query (88.6 bits)
NIT1_MOUSE / Q8VDK1 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Mus musculus (Mouse) (see 7 papers)
Q8VDK1 deaminated glutathione amidase (EC 3.5.1.128) from Mus musculus (see paper)
29% identity, 91% coverage of query (87.4 bits)
AO356_14225 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2C3
32% identity, 91% coverage of query (87.0 bits)
NIT1_HUMAN / Q86X76 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Homo sapiens (Human) (see paper)
31% identity, 91% coverage of query (86.7 bits)
nitA / CCN27136.1 nitrilase from Rhodococcus ruber (see paper)
28% identity, 88% coverage of query (85.5 bits)
A4LA85 nitrilase (EC 3.5.5.1) from Rhodococcus rhodochrous (see paper)
28% identity, 88% coverage of query (85.5 bits)
nitA / CCN27135.1 nitrilase from Rhodococcus ruber (see paper)
28% identity, 88% coverage of query (85.5 bits)
Q6RWE0 nitrilase (EC 3.5.5.1) from uncultured organism (see paper)
28% identity, 88% coverage of query (85.1 bits)
Q03217 Aliphatic nitrilase (EC 3.5.5.7) from Rhodococcus rhodochrous (see 3 papers)
nitA / GB|BAA01994.1 aliphatic nitrilase; EC 3.5.5.7 from Rhodococcus rhodochrous (see paper)
nitA / BAA11037.1 nitrilase from Rhodococcus rhodochrous (see paper)
28% identity, 88% coverage of query (85.1 bits)
Q03217 Aliphatic nitrilase; EC 3.5.5.7 from Rhodococcus rhodochrous
28% identity, 88% coverage of query (85.1 bits)
nitA / CCN27134.1 nitrilase from Rhodococcus rhodochrous (see paper)
28% identity, 88% coverage of query (83.6 bits)
NIT3_YEAST / P49954 Omega-amidase NIT3; Nitrilase homolog 2; EC 3.5.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
27% identity, 90% coverage of query (83.6 bits)
Q19437 beta-ureidopropionase (EC 3.5.1.6) from Caenorhabditis elegans (see paper)
28% identity, 90% coverage of query (82.8 bits)
AMIE_RHOER / Q01360 Aliphatic amidase; Acylamide amidohydrolase; Wide spectrum amidase; EC 3.5.1.4 from Rhodococcus erythropolis (Arthrobacter picolinophilus)
30% identity, 78% coverage of query (82.8 bits)
AO353_07425 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2E3
31% identity, 90% coverage of query (81.3 bits)
BUP1_RAT / Q03248 Beta-ureidopropionase; Beta-alanine synthase; N-carbamoyl-beta-alanine amidohydrolase; EC 3.5.1.6 from Rattus norvegicus (Rat) (see 2 papers)
27% identity, 91% coverage of query (80.9 bits)
E1A0Z9 nitrilase (EC 3.5.5.1); Aliphatic nitrilase (EC 3.5.5.7) from uncultured bacterium (see paper)
30% identity, 90% coverage of query (80.5 bits)
NFT1_CAEEL / O76463 Nitrilase and fragile histidine triad fusion protein NitFhit; EC 3.6.1.29; EC 3.5.-.- from Caenorhabditis elegans (see 2 papers)
26% identity, 90% coverage of query (79.7 bits)
NIT4A / Q5QGZ8 β-cyano-L-alanine nitrilase (EC 3.5.5.4; EC 4.2.1.65) from Lupinus angustifolius (see paper)
NRL4A_LUPAN / Q5QGZ8 Bifunctional nitrilase/nitrile hydratase NIT4A; LaNIT4A; 3-cyanoalanine hydratase; Cyanoalanine nitrilase A; EC 3.5.5.4; EC 4.2.1.65 from Lupinus angustifolius (Narrow-leaved blue lupine) (see paper)
Q5QGZ8 cyanoalanine nitrilase (EC 3.5.5.4); 3-Cyanoalanine hydratase (EC 4.2.1.65) from Lupinus angustifolius (see paper)
28% identity, 87% coverage of query (79.3 bits)
Q5KR04 N-carbamoylputrescine amidase (EC 3.5.1.53) from Selenomonas ruminantium (see paper)
28% identity, 92% coverage of query (79.0 bits)
A0A0H3LIT0 nitrilase (EC 3.5.5.1) from Bordetella bronchiseptica (see paper)
28% identity, 88% coverage of query (78.6 bits)
W6R265 cyanoalanine nitrilase (EC 3.5.5.4) from Pseudomonas oleovorans (see paper)
29% identity, 87% coverage of query (78.6 bits)
Q0PIV8 nitrilase (EC 3.5.5.1) from Aeribacillus pallidus (see 2 papers)
27% identity, 88% coverage of query (78.2 bits)
NITR2_BRADU / Q89PT3 Nitrilase blr3397; EC 3.5.5.7 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
32% identity, 87% coverage of query (78.2 bits)
NFT1_DROME / O76464 Nitrilase and fragile histidine triad fusion protein NitFhit; NFT-1 protein; EC 3.6.1.29; EC 3.5.-.- from Drosophila melanogaster (Fruit fly) (see paper)
28% identity, 93% coverage of query (78.2 bits)
NIT_RHIFH / G9AIU0 Aliphatic nitrilase; NitSf; EC 3.5.5.1 from Rhizobium fredii (strain HH103) (Sinorhizobium fredii) (see paper)
29% identity, 87% coverage of query (77.4 bits)
NADE_ACIAD / Q6F8K4 Glutamine-dependent NAD(+) synthetase; NAD synthetase; NAD(+) synthase [glutamine-hydrolyzing]; EC 6.3.5.1 from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
26% identity, 86% coverage of query (77.4 bits)
UPB1 / Q9UBR1 β-ureidopropionase (EC 3.5.1.6) from Homo sapiens (see 2 papers)
BUP1_HUMAN / Q9UBR1 Beta-ureidopropionase; BUP-1; Beta-alanine synthase; N-carbamoyl-beta-alanine amidohydrolase; EC 3.5.1.6 from Homo sapiens (Human) (see 7 papers)
28% identity, 79% coverage of query (76.3 bits)
K9NKH3 nitrilase (EC 3.5.5.1) from Pseudomonas sp. (see paper)
29% identity, 88% coverage of query (76.3 bits)
P82605 nitrilase (EC 3.5.5.1) from Bacillus sp. OxB-1 (see paper)
26% identity, 85% coverage of query (75.9 bits)
AMIE_PSEAE / P11436 Aliphatic amidase; Acylamide amidohydrolase; EC 3.5.1.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
P11436 amidase (EC 3.5.1.4) from Pseudomonas aeruginosa (see paper)
31% identity, 78% coverage of query (75.1 bits)
NIT4B / Q3LRV4 β-cyano-L-alanine nitrilase (EC 3.5.5.4; EC 4.2.1.65) from Lupinus angustifolius (see paper)
NRL4B_LUPAN / Q3LRV4 Bifunctional nitrilase/nitrile hydratase NIT4B; LaNIT4B; 3-cyanoalanine hydratase; Cyanoalanine nitrilase B; EC 3.5.5.4; EC 4.2.1.65 from Lupinus angustifolius (Narrow-leaved blue lupine) (see paper)
Q3LRV4 cyanoalanine nitrilase (EC 3.5.5.4) from Lupinus angustifolius (see paper)
27% identity, 87% coverage of query (75.1 bits)
Q1W2L4 nitrilase (EC 3.5.5.1) from Acidovorax facilis (see 3 papers)
28% identity, 91% coverage of query (75.1 bits)
AMIF_HELPY / O25836 Formamidase; Formamide amidohydrolase; EC 3.5.1.49 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see 2 papers)
28% identity, 75% coverage of query (74.7 bits)
NIT1_SYNYG / P0DP66 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; syNit1; EC 3.5.1.128 from Synechocystis sp. (strain PCC 6803 / GT-S) (see paper)
P0DP66 deaminated glutathione amidase (EC 3.5.1.128) from Synechocystis sp. PCC 6803 (see paper)
28% identity, 86% coverage of query (73.9 bits)
YHCX_BACSU / P54608 Probable hydrolase YhcX; EC 3.5.-.- from Bacillus subtilis (strain 168) (see paper)
26% identity, 91% coverage of query (73.6 bits)
Q1I7X1 nitrilase (EC 3.5.5.1) from Pseudomonas entomophila (see paper)
30% identity, 91% coverage of query (73.2 bits)
YAFV_STAA4 / P0DP65 Omega-amidase YafV; saYafV; EC 3.5.1.3 from Staphylococcus aureus (strain 04-02981) (see paper)
26% identity, 90% coverage of query (72.8 bits)
NRL4B_TOBAC / Q42966 Bifunctional nitrilase/nitrile hydratase NIT4B; TNIT4B; Cyanoalanine nitrilase B; Nitrilase 4B; EC 3.5.5.1; EC 3.5.5.4 from Nicotiana tabacum (Common tobacco) (see 2 papers)
27% identity, 87% coverage of query (72.8 bits)
Q4KCL8 Aliphatic nitrilase (EC 3.5.5.7) from Pseudomonas fluorescens (see paper)
28% identity, 91% coverage of query (72.0 bits)
NRL4A_TOBAC / Q42965 Bifunctional nitrilase/nitrile hydratase NIT4A; TNIT4A; 3-cyanoalanine hydratase; Cyanoalanine nitrilase A; Nitrilase 4A; EC 3.5.5.1; EC 3.5.5.4; EC 4.2.1.65 from Nicotiana tabacum (Common tobacco) (see 3 papers)
27% identity, 87% coverage of query (72.0 bits)
B3GNT7 nitrilase (EC 3.5.5.1) from Bacillus pumilus (see paper)
28% identity, 82% coverage of query (71.6 bits)
A4YWK0 Aliphatic nitrilase (EC 3.5.5.7) from Bradyrhizobium sp. ORS 278 (see paper)
28% identity, 90% coverage of query (71.2 bits)
Q59329 nitrilase (EC 3.5.5.1); Aliphatic nitrilase (EC 3.5.5.7) from Comamonas testosteroni (see 2 papers)
nitA / AAA82085.1 aliphatic nitrilase from Comamonas testosteroni (see paper)
27% identity, 87% coverage of query (71.2 bits)
NIT1 / P32961 Nit1 (EC 3.5.5.1; EC 4.2.1.84) from Arabidopsis thaliana (see 5 papers)
NRL1_ARATH / P32961 Nitrilase 1; EC 3.5.5.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
P32961 deaminated glutathione amidase (EC 3.5.1.128); nitrilase (EC 3.5.5.1) from Arabidopsis thaliana (see 6 papers)
29% identity, 88% coverage of query (70.5 bits)
A1ZD79 nitrilase (EC 3.5.5.1) from Microscilla marina (see paper)
26% identity, 88% coverage of query (68.9 bits)
A3DHT2 nitrilase (EC 3.5.5.1) from Acetivibrio thermocellus (see paper)
26% identity, 91% coverage of query (67.8 bits)
Q02068 Aliphatic nitrilase (EC 3.5.5.7) from Rhodococcus rhodochrous (see paper)
26% identity, 83% coverage of query (67.8 bits)
R0H4W3 nitrilase (EC 3.5.5.1) from Capsella rubella (see paper)
27% identity, 88% coverage of query (67.8 bits)
Q02068 Aliphatic nitrilase; EC 3.5.5.7 from Rhodococcus rhodochrous
26% identity, 83% coverage of query (67.8 bits)
B8C1M9 nitrilase (EC 3.5.5.1) from Thalassiosira pseudonana (see paper)
26% identity, 89% coverage of query (67.4 bits)
A3QYW3 nitrilase (EC 3.5.5.1) from Brassica rapa (see paper)
27% identity, 87% coverage of query (67.4 bits)
5khaA / V5VHL3 Structure of glutamine-dependent NAD+ synthetase from acinetobacter baumannii in complex with adenosine diphosphate (adp)
25% identity, 82% coverage of query (63.9 bits)
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Lawrence Berkeley National Laboratory