Sites on a Tree

 

Searching for up to 100 curated homologs for H281DRAFT_06355 FitnessBrowser__Burk376:H281DRAFT_06355 (346 a.a.)

Found high-coverage hits (≥70%) to 30 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

proH / Q9AQI1 subunit of 4-oxalomesaconate hydratase (EC 4.2.1.83) from Pseudomonas straminea (see 3 papers)
proH / BAB21455.1 4-Oxalomesaconate hydratase from Pseudomonas straminea (see 2 papers)
    63% identity, 99% coverage of query (471 bits)

pmdE / Q93PS9 4-carboxy-2-hydroxymuconate hydratase (EC 4.2.1.83) from Comamonas testosteroni (see paper)
Q93PS9 4-oxalomesaconate hydratase (EC 4.2.1.83) from Comamonas testosteroni (see paper)
    63% identity, 99% coverage of query (468 bits)

LIGJ_SPHSK / G2IQQ5 2-keto-4-carboxy-3-hexenedioate hydratase; KCH hydratase; EC 4.2.1.- from Sphingobium sp. (strain NBRC 103272 / SYK-6) (see 3 papers)
ligJ / BAA97116.1 4-oxalomesaconate hydratase from Sphingomonas paucimobilis (see 3 papers)
ligJ / BAB88741.1 4-oxalomesaconate hydratase from Sphingomonas paucimobilis (see 5 papers)
    64% identity, 99% coverage of query (461 bits)

6dxsA / G2IQQ5 Crystal structure of the ligj hydratase e284q mutant substrate complex with (3z)-2-keto-4-carboxy-3-hexenedioate (see paper)
    63% identity, 100% coverage of query (459 bits)

6dxqA Crystal structure of the ligj hydratase product complex with 4- carboxy-4-hydroxy-2-oxoadipate
    64% identity, 99% coverage of query (459 bits)

2gwgA / Q6N0R4 Crystal structure of 4-oxalomesaconate hydratase, ligj, from rhodopseudomonas palustris, northeast structural genomics target rpr66.
    59% identity, 100% coverage of query (434 bits)

ligY / Q9Z9I9 OH-DDVA meta-cleavage compound hydrolase from Sphingomonas paucimobilis (see paper)
    37% identity, 95% coverage of query (213 bits)

6e6iA Crystal structure of 4-methyl hopda bound to ligy from sphingobium sp. Strain syk-6
    37% identity, 95% coverage of query (213 bits)

5vn5C / G2IN02 Crystal structure of ligy from sphingobium sp. Strain syk-6 (see paper)
    37% identity, 95% coverage of query (212 bits)

Build an alignment

Build an alignment for H281DRAFT_06355 and 9 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

ligW2 / Q84IP8 5-carboxyvanillate decarboxylase from Sphingomonas paucimobilis (see 2 papers)
    26% identity, 80% coverage of query (76.3 bits)

nbaD / A4IT47 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase (EC 4.1.1.45) from Geobacillus thermodenitrificans (strain NG80-2) (see paper)
    26% identity, 82% coverage of query (75.5 bits)

4qroA / Q12BV1 Crystal structure of dihydroxybenzoic acid decarbboxylase bpro_2061 (target efi-500288) from polaromonas sp. Js666 with bound manganese and an inhibitor, 2-nitroresorcinol
    26% identity, 83% coverage of query (71.2 bits)

GRDC_POLSJ / Q12BV1 Gamma-resorcylate decarboxylase; Gamma-RSD; 2,6-dihydroxybenzoate decarboxylase; 2,6-DHBD; EC 4.1.1.103 from Polaromonas sp. (strain JS666 / ATCC BAA-500) (see paper)
Q12BV1 gamma-resorcylate decarboxylase (EC 4.1.1.103) from Polaromonas sp. JS666 (see paper)
    26% identity, 83% coverage of query (71.2 bits)

ACMSD / Q8TDX5 aminocarboxymuconate-semialdehyde decarboxylase (EC 4.1.1.45) from Homo sapiens (see 2 papers)
ACMSD_HUMAN / Q8TDX5 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase; Picolinate carboxylase; EC 4.1.1.45 from Homo sapiens (Human) (see 2 papers)
Q8TDX5 aminocarboxymuconate-semialdehyde decarboxylase (EC 4.1.1.45) from Homo sapiens (see 5 papers)
    25% identity, 71% coverage of query (70.9 bits)

4qrnA / Q2GA79 High-resolution crystal structure of 5-carboxyvanillate decarboxylase (target efi-505250) from novosphingobium aromaticivorans dsm 12444 complexed with manganese and 4-hydroxy-3-methoxy-5-nitrobenzoic acid
    25% identity, 77% coverage of query (68.6 bits)

4infA Crystal structure of amidohydrolase saro_0799 (target efi-505250) from novosphingobium aromaticivorans dsm 12444 with bound calcium
    25% identity, 77% coverage of query (68.6 bits)

7a19A / P80402 2,3-dihydroxybenzoate decarboxylase of aspergillus oryzae (see paper)
    23% identity, 82% coverage of query (65.1 bits)

7wjrA Crystal structure of dihydroxybenzoate decarboxylase mutant a63s from aspergillus oryzae in complex with catechol
    23% identity, 82% coverage of query (63.9 bits)

8h41B / P0CT50 Crystal structure of a decarboxylase from trichosporon moniliiforme in complex with o-nitrophenol
    21% identity, 96% coverage of query (60.8 bits)

6jqxA Crystal structure of a hydrogenase from trichosporon moniliiforme
    21% identity, 87% coverage of query (60.5 bits)

6jqwA Crystal structure of a hydrogenase from trichosporon moniliiforme
    21% identity, 87% coverage of query (60.5 bits)

SDC_CUTMO / P0CT50 Salicylate decarboxylase; Salicylic acid decarboxylase; EC 4.1.1.91 from Cutaneotrichosporon moniliiforme (Yeast) (Trichosporon moniliiforme) (see paper)
P0CT50 salicylate decarboxylase (EC 4.1.1.91) from Cutaneotrichosporon moniliiforme (see paper)
    21% identity, 87% coverage of query (60.1 bits)

6m53B / N1S495 Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from fusarium oxysporum (see paper)
    21% identity, 81% coverage of query (58.2 bits)

X0JT48 o-pyrocatechuate decarboxylase (EC 4.1.1.46) from Fusarium odoratissimum NRRL 54006 (see paper)
    21% identity, 81% coverage of query (58.2 bits)

7bp1A Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from fusarium oxysporum in complex with catechol
    21% identity, 81% coverage of query (58.2 bits)

7bpcA Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from fusarium oxysporum in complex with 2,5-dhba
    21% identity, 81% coverage of query (58.2 bits)

3ij6A / Q5FK79 Crystal structure of an uncharacterized metal-dependent hydrolase from lactobacillus acidophilus
    21% identity, 71% coverage of query (57.8 bits)

AR281_ARTOA / G1XU02 6-methylsalicylic acid decarboxylase; Sesquiterpenyl epoxy-cyclohexenoids cluster protein AOL_s00215g281; SECs cluster protein AOL_s00215g281; EC 4.1.1.52 from Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491) (Nematode-trapping fungus) (Didymozoophaga oligospora) (see 3 papers)
G1XU02 6-methylsalicylate decarboxylase (EC 4.1.1.52) from Orbilia oligospora (see paper)
    25% identity, 83% coverage of query (50.1 bits)

T1PRE6 6-methylsalicylate decarboxylase (EC 4.1.1.52) from Aspergillus clavatus (see paper)
    23% identity, 82% coverage of query (48.5 bits)

patG / A1CFL4 6-methylsalicylate decarboxylase (EC 4.1.1.52) from Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) (see 2 papers)
PATG_ASPCL / A1CFL4 6-methylsalicylic acid decarboxylase; Patulin synthesis protein G; EC 4.1.1.52 from Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) (see 4 papers)
    23% identity, 82% coverage of query (48.1 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory