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Searching for up to 100 curated homologs for HSERO_RS00870 FitnessBrowser__HerbieS:HSERO_RS00870 (378 a.a.)

Found high-coverage hits (≥70%) to 43 curated proteins.

Removed hits that are identical to the query, leaving 42

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Ac3H11_2396 ABC transporter for glutamine/isoleucine/leucine/phenylalanine/proline/serine/tyrosine, substrate-binding component from Acidovorax sp. GW101-3H11
    53% identity, 93% coverage of query (357 bits)

AZOBR_RS08260 L-proline and D-alanine ABC transporter, substrate-binding component from Azospirillum brasilense Sp245
    34% identity, 90% coverage of query (181 bits)

BRAC_PSEAE / P21175 Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein; LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
TC 3.A.1.4.8 / P21175 Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, component of Branched chain amino acid uptake transporter. Transports alanine from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    32% identity, 97% coverage of query (179 bits)

LIVB3_RHIJ3 / Q1MDE9 Leu/Ile/Val-binding protein BraC3 from Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841) (Rhizobium leguminosarum bv. viciae) (see paper)
    32% identity, 95% coverage of query (174 bits)

3ip5A / Q7CX36 Structure of atu2422-gaba receptor in complex with alanine (see paper)
    37% identity, 90% coverage of query (174 bits)

3ip9A Structure of atu2422-gaba receptor in complex with gaba
    37% identity, 90% coverage of query (174 bits)

3ip7A Structure of atu2422-gaba receptor in complex with valine
    37% identity, 90% coverage of query (174 bits)

3ip6A Structure of atu2422-gaba receptor in complex with proline
    37% identity, 90% coverage of query (174 bits)

3ipcA Structure of atu2422-gaba f77a mutant receptor in complex with leucine
    37% identity, 90% coverage of query (172 bits)

Pf6N2E2_2921 ABC transporter for L-leucine/L-phenylalanine/D-alanine, substrate-binding component LivK from Pseudomonas fluorescens FW300-N2E2
    33% identity, 98% coverage of query (169 bits)

TC 3.A.1.4.3 / Q9L3M3 BraC, component of General L- (and D-)amino acid uptake porter (transports acidic, basic, polar, semipolar and hydrophobic amino acids). The amino and carboxyl groups do not need to be α since γ-aminobutyric acid (GABA) is a substrate. The system may function with additional binding proteins since L-alanine uptake is not dependent on BraC from Rhizobium leguminosarum (biovar viciae) (see 2 papers)
braC / CAB75553.1 periplasmic binding protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    34% identity, 90% coverage of query (159 bits)

AABP_PSEAE / Q9HUQ0 Amino acid binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    33% identity, 98% coverage of query (158 bits)

4n0qB / Q8YEE8 Crystal structure of an abc transporter, substrate-binding protein from brucella melitensis 16m in complex with l-leucine using a crystal grown in a crystal former (microlytic)
    31% identity, 92% coverage of query (157 bits)

HrbC / b3458 L-leucine/L-phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli K-12 substr. MG1655 (see 17 papers)
livK / P04816 L-leucine/L-phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli (strain K12) (see 16 papers)
TC 3.A.1.4.1 / P04816 Livk aka B3458, component of Leucine; leucine/isoleucine/valine porter from Escherichia coli (see 9 papers)
livK leucine-specific-binding protein LivK from Escherichia coli K12 (see 9 papers)
    31% identity, 95% coverage of query (150 bits)

1usiA / P04816 L-leucine-binding protein with phenylalanine bound (see paper)
    30% identity, 90% coverage of query (145 bits)

1uskA L-leucine-binding protein with leucine bound
    30% identity, 90% coverage of query (145 bits)

Build an alignment

Build an alignment for HSERO_RS00870 and 16 homologs with ≥ 30% identity

Select sequences

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Change minimum %identity:

Additional hits (identity < 30%)

LivJ / b3460 branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli K-12 substr. MG1655 (see 12 papers)
LivJ / P0AD96 branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli (strain K12) (see 12 papers)
TC 3.A.1.4.1 / P0AD96 Leu/Ile/Val-binding protein LivJ aka B3460 aka LIV-BP, component of Leucine; leucine/isoleucine/valine porter from Escherichia coli (see 9 papers)
    28% identity, 97% coverage of query (143 bits)

1z16A / P0AD96 Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound leucine (see paper)
    28% identity, 90% coverage of query (139 bits)

1z18A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound valine
    28% identity, 90% coverage of query (139 bits)

1z17A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound ligand isoleucine
    28% identity, 90% coverage of query (139 bits)

TC 3.A.1.4.10 / Q8DQI1 ABC transporter substrate-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM from Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
    32% identity, 88% coverage of query (131 bits)

4mlcA / B8FZ96 Abc transporter substrate-binding protein fromdesulfitobacterium hafniense
    28% identity, 84% coverage of query (130 bits)

4gnrA / J9PBT7 1.0 angstrom resolution crystal structure of the branched-chain amino acid transporter substrate binding protein livj from streptococcus pneumoniae str. Canada mdr_19a in complex with isoleucine
    31% identity, 87% coverage of query (129 bits)

4q6bA Crystal structure of abc transporter substrate-binding protein fromdesulfitobacterium hafniense complex with leu
    28% identity, 84% coverage of query (129 bits)

3td9A / Q9X0L9 Crystal structure of a leucine binding protein livk (tm1135) from thermotoga maritima msb8 at 1.90 a resolution
    29% identity, 89% coverage of query (108 bits)

4q6wA / Q7VS30 Crystal structure of periplasmic binding protein type 1 from bordetella pertussis tohama i complexed with 3-hydroxy benzoic acid
    29% identity, 90% coverage of query (102 bits)

4eygB / Q13AR6 Crystal structure of solute binding protein of abc transporter from rhodopseudomonas palustris bisb5 in complex with vanillic acid (see paper)
    27% identity, 78% coverage of query (66.2 bits)

PGA1_c12670 D-lactate transporter, substrate binding component from Phaeobacter inhibens BS107
    24% identity, 86% coverage of query (63.2 bits)

4dqdA / Q2IR47 The crystal structure of a transporter in complex with 3-phenylpyruvic acid (see paper)
    22% identity, 78% coverage of query (60.8 bits)

3sg0A The crystal structure of an extracellular ligand-binding receptor from rhodopseudomonas palustris haa2
    22% identity, 78% coverage of query (60.8 bits)

TC 3.A.1.4.6 / Q8YVY4 NatB, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (see paper)
    26% identity, 87% coverage of query (56.2 bits)

4nqrA / Q3MFZ5 The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with alanine
    23% identity, 85% coverage of query (55.1 bits)

4rdcA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with proline
    23% identity, 85% coverage of query (55.1 bits)

4qymA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with methionine
    23% identity, 85% coverage of query (55.1 bits)

4otzA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with cystein
    23% identity, 85% coverage of query (55.1 bits)

4og2A The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with leucine
    23% identity, 85% coverage of query (55.1 bits)

4oatA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with isoleucine.
    23% identity, 85% coverage of query (55.1 bits)

4nv3A The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with valine.
    23% identity, 85% coverage of query (55.1 bits)

4rv5A The crystal structure of a solute-binding protein from anabaena variabilis atcc 29413 in complex with pyruvic acid
    23% identity, 85% coverage of query (53.5 bits)

4obbA The crystal structure of a solute-binding protein from anabaena variabilis atcc 29413 in complex with (3s)-3-methyl-2-oxopentanoic acid.
    23% identity, 85% coverage of query (53.5 bits)

TC 3.A.1.4.5 / Q8NRW1 UrtB, component of The high affinity urea/thiourea/hydroxyurea porter from Corynebacterium glutamicum (see paper)
    23% identity, 88% coverage of query (51.6 bits)

3lkbA / Q5SL42 Crystal structure of a branched chain amino acid abc transporter from thermus thermophilus with bound valine
    20% identity, 93% coverage of query (48.9 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory