Sites on a Tree

 

Searching for up to 100 curated homologs for N515DRAFT_0566 FitnessBrowser__Dyella79:N515DRAFT_0566 (82 a.a.)

Found high-coverage hits (≥70%) to 25 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

2pc6A / Q82UZ2 Crystal structure of putative acetolactate synthase- small subunit from nitrosomonas europaea (see paper)
    55% identity, 98% coverage of query (100 bits)

ilvH / Q0KCU3 acetohydroxyacid synthase subunit H (EC 2.2.1.6) from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (see paper)
    52% identity, 89% coverage of query (89.7 bits)

BrnP / b0078 acetolactate synthase / acetohydroxybutanoate synthase, regulatory subunit (EC 2.2.1.6) from Escherichia coli K-12 substr. MG1655 (see 10 papers)
ilvH / P00894 acetolactate synthase / acetohydroxybutanoate synthase, regulatory subunit (EC 2.2.1.6) from Escherichia coli (strain K12) (see 10 papers)
P00894 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Escherichia coli (see paper)
ilvH / GB|BAB96647.2 acetolactate synthase, small subunit; EC 2.2.1.6 from Escherichia coli (see paper)
    55% identity, 91% coverage of query (86.3 bits)

ILV6_SCHPO / O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    47% identity, 88% coverage of query (74.3 bits)

ILVH_MYCTU / P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
P9WKJ3 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Mycobacterium tuberculosis (see paper)
    48% identity, 87% coverage of query (73.6 bits)

ilvN / AAB38427.1 acetolactate synthase from Mycobacterium avium (see paper)
    46% identity, 87% coverage of query (72.8 bits)

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
    46% identity, 87% coverage of query (72.8 bits)

ilvN / O08354 acetohydroxy-acid synthase small subunit (EC 2.2.1.6) from Methanococcus aeolicus (see 2 papers)
    42% identity, 87% coverage of query (70.9 bits)

G4NC97 acetolactate synthase (subunit 2/2) (EC 2.2.1.6) from Pyricularia oryzae (see paper)
    47% identity, 88% coverage of query (69.3 bits)

AN4430 small subunit of acetolactate synthase from Emericella nidulans (see paper)
    47% identity, 88% coverage of query (68.6 bits)

ILV6_YEAST / P25605 Acetolactate synthase small subunit, mitochondrial; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
    47% identity, 88% coverage of query (68.6 bits)

6u9dL / P25605 Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
    46% identity, 88% coverage of query (67.8 bits)

6vz8F / Q93YZ7 Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
    40% identity, 95% coverage of query (67.8 bits)

6wo1B Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit
    46% identity, 88% coverage of query (67.8 bits)

ILVH2_ARATH / Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
    40% identity, 95% coverage of query (67.0 bits)

ILVH1_ARATH / Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 6 papers)
    40% identity, 95% coverage of query (66.6 bits)

ILVH_NICPL / Q9SMC2 Acetolactate synthase small subunit 1, chloroplastic; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco) (see paper)
    45% identity, 87% coverage of query (64.3 bits)

6vz8G / Q93YZ7 Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
    45% identity, 87% coverage of query (63.2 bits)

Echvi_2057 acetohydroxybutanoate synthase regulatory subunit (EC 2.2.1.6) from Echinicola vietnamensis KMM 6221, DSM 17526
    37% identity, 87% coverage of query (58.5 bits)

Q8RL87 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Geobacillus stearothermophilus (see paper)
ilvN / AAL99357.1 acetohydroxy acid synthase small subunit from Geobacillus stearothermophilus (see paper)
    36% identity, 94% coverage of query (54.7 bits)

Build an alignment

Build an alignment for N515DRAFT_0566 and 20 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

CA265_RS15810 acetohydroxybutanoate synthase regulatory subunit (EC 2.2.1.6) from Pedobacter sp. GW460-11-11-14-LB5
    29% identity, 98% coverage of query (51.2 bits)

5yppE / P0ADF8 Crystal structure of ilvn.Val-1a (see paper)
    33% identity, 84% coverage of query (47.4 bits)

IlvN / b3670 acetohydroxy acid synthase I subunit IlvN (EC 2.2.1.6) from Escherichia coli K-12 substr. MG1655 (see 9 papers)
ilvN / P0ADF8 acetohydroxy acid synthase I subunit IlvN (EC 2.2.1.6) from Escherichia coli (strain K12) (see 6 papers)
P0ADF8 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Escherichia coli (see paper)
ilvN / RF|NP_418126 acetolactate synthase, small subunit; EC 2.2.1.6 from Escherichia coli K12 (see 3 papers)
    33% identity, 84% coverage of query (47.4 bits)

5yumA Crystallographic structures of ilvn.Val/ile complexes:conformational selectivity for feedback inhibition of ahass
    33% identity, 84% coverage of query (47.4 bits)

BT2076 acetohydroxybutanoate synthase regulatory subunit (EC 2.2.1.6) from Bacteroides thetaiotaomicron VPI-5482
    30% identity, 89% coverage of query (45.4 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory