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Searching for up to 100 curated homologs for PP_1071 FitnessBrowser__Putida:PP_1071 (306 a.a.)

Found high-coverage hits (≥70%) to 53 curated proteins.

Removed hits that are identical to the query, leaving 52

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO353_16290 ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E3
    78% identity, 100% coverage of query (483 bits)

Pf1N1B4_771 ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N1B4
    80% identity, 100% coverage of query (477 bits)

GASBP_PSEAE / Q9I402 L-glutamate/L-aspartate-binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
TC 3.A.1.3.22 / Q9I402 Probable binding protein component of ABC transporter, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    76% identity, 99% coverage of query (472 bits)

PfGW456L13_4770 ABC transporter for L-Asparagine and possibly other L-amino acids, periplasmic substrate-binding component from Pseudomonas fluorescens GW456-L13
    80% identity, 100% coverage of query (472 bits)

2ia4B / A0A0H2UXX1 Crystal structure of novel amino acid binding protein from shigella flexneri
    59% identity, 92% coverage of query (332 bits)

2vhaA Debp
    60% identity, 89% coverage of query (331 bits)

GltI / b0655 glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
GltI / P37902 glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli (strain K12) (see 3 papers)
GLTI_ECOLI / P37902 Glutamate/aspartate import solute-binding protein from Escherichia coli (strain K12) (see 3 papers)
TC 3.A.1.3.4 / P37902 YBEJ aka GltI aka B0655, component of Glutamate/aspartate porter from Escherichia coli (see 6 papers)
gltI / GB|BAA35307.2 glutamate-aspartate periplasmic-binding protein from Escherichia coli K12 (see 6 papers)
    60% identity, 89% coverage of query (327 bits)

8ovoA / P37902,P42212 X-ray structure of the sf-iglusnfr-s72a in complex with l-aspartate
    59% identity, 80% coverage of query (285 bits)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
    34% identity, 76% coverage of query (97.8 bits)

GLNH_BACSU / O34563 ABC transporter glutamine-binding protein GlnH from Bacillus subtilis (strain 168) (see paper)
    30% identity, 89% coverage of query (95.9 bits)

4zv2A An ancestral arginine-binding protein bound to glutamine
    34% identity, 76% coverage of query (95.5 bits)

5eyfB / Q3XZW5 Crystal structure of solute-binding protein from enterococcus faecium with bound glutamate
    32% identity, 78% coverage of query (92.4 bits)

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
    33% identity, 79% coverage of query (84.3 bits)

Build an alignment

Build an alignment for PP_1071 and 13 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

6svfA / Q9WZ62 Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
    28% identity, 79% coverage of query (74.7 bits)

TC 3.A.1.3.16 / Q0P9X8 PEB1A, component of Uptake system for glutamate and aspartate from Campylobacter jejuni (see 2 papers)
peb1A / RF|YP_002344319.1 major cell-binding factor from Campylobacter jejuni (see 3 papers)
peb1 / AAA02919.1 major cell-binding factor from Campylobacter jejuni (see paper)
    29% identity, 84% coverage of query (72.4 bits)

2yjpA / Q5F5B5 Crystal structure of the solute receptors for l-cysteine of neisseria gonorrhoeae (see paper)
    28% identity, 78% coverage of query (69.7 bits)

2v25A / Q0P9X8 Structure of the campylobacter jejuni antigen peb1a, an aspartate and glutamate receptor with bound aspartate (see paper)
    28% identity, 79% coverage of query (69.3 bits)

4ymxA / Q8RCC4 Crystal structure of the substrate binding protein of an amino acid abc transporter (see paper)
    27% identity, 77% coverage of query (68.2 bits)

TcyJ / b1920 cystine ABC transporter periplasmic binding protein (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 12 papers)
tcyJ / P0AEM9 cystine ABC transporter periplasmic binding protein (EC 7.4.2.12) from Escherichia coli (strain K12) (see 13 papers)
TCYJ_ECOLI / P0AEM9 L-cystine-binding protein TcyJ; CBP; Protein FliY; Sulfate starvation-induced protein 7; SSI7 from Escherichia coli (strain K12) (see 6 papers)
TC 3.A.1.3.10 / P0AEM9 Cystine-binding periplasmic protein FLIY aka CysX aka B1920, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 7 papers)
    26% identity, 88% coverage of query (63.2 bits)

Pf6N2E2_5402 ABC transporter for D-Alanine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    24% identity, 79% coverage of query (62.8 bits)

4z9nB / A0A0M3KL33 Abc transporter / periplasmic binding protein from brucella ovis with glutathione bound
    26% identity, 72% coverage of query (59.3 bits)

cjaA / CAJ20048.1 glutamine-binding protein from Campylobacter jejuni (see paper)
    24% identity, 88% coverage of query (57.4 bits)

AO356_00480 ABC transporter for D-Glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2C3
    24% identity, 88% coverage of query (57.0 bits)

4h5fA Crystal structure of an amino acid abc transporter substrate-binding protein from streptococcus pneumoniae canada mdr_19a bound to l- arginine, form 1
    25% identity, 73% coverage of query (56.6 bits)

AAPJ_RHIJ3 / Q52812 General L-amino acid-binding periplasmic protein AapJ from Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841) (Rhizobium leguminosarum bv. viciae) (see 2 papers)
TC 3.A.1.3.8 / Q52812 AapJ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) from Rhizobium leguminosarum (biovar viciae) (see 2 papers)
aapJ / CAA57933.1 general amino acid ABC type transporter from Rhizobium leguminosarum (see paper)
    26% identity, 77% coverage of query (56.6 bits)

ArtJ / b0860 L-arginine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see paper)
ArtJ / P30860 L-arginine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli (strain K12) (see paper)
ARTJ_ECOLI / P30860 ABC transporter arginine-binding protein 1 from Escherichia coli (strain K12) (see paper)
TC 3.A.1.3.3 / P30860 ArtJ aka B0860, component of Arginine porter from Escherichia coli (see 7 papers)
artJ / GB|BAA35574.1 arginine ABC transporter, periplasmic arginine-binding protein ArtJ from Escherichia coli K12 (see 8 papers)
    28% identity, 76% coverage of query (55.1 bits)

2y7iA / Q8ZKA9 Structural basis for high arginine specificity in salmonella typhimurium periplasmic binding protein stm4351. (see paper)
    25% identity, 76% coverage of query (55.1 bits)

6h2tA / P96257 Glnh bound to glu, mycobacterium tuberculosis (see paper)
    24% identity, 83% coverage of query (55.1 bits)

6h20A Glnh bound to asn, mycobacterium tuberculosis
    24% identity, 83% coverage of query (55.1 bits)

6h1uA Glnh bound to asp, mycobacterium tuberculosis
    24% identity, 83% coverage of query (55.1 bits)

AO353_21710 ABC transporter for D-glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E3
    25% identity, 91% coverage of query (54.3 bits)

ARGT_SALTY / P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 6 papers)
argT / RF|NP_461297.1 lysine/arginine/ornithine ABC transporter, periplasmic lysine/arginine/ornithine-binding protein ArgT from Salmonella enterica subsp. enterica serovar Typhimurium (see 4 papers)
    23% identity, 76% coverage of query (53.5 bits)

Pf6N2E2_2053 ABC transporter for D-Glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    24% identity, 83% coverage of query (53.5 bits)

SMc02118 ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, periplasmic substrate-binding component from Sinorhizobium meliloti 1021
    24% identity, 78% coverage of query (53.5 bits)

YXEM_BACSU / P54952 Probable amino-acid-binding protein YxeM from Bacillus subtilis (strain 168) (see 2 papers)
    24% identity, 78% coverage of query (53.5 bits)

5l9oB Crystal structure of agrobacterium tumefaciens c58 strain pbp soca in complex with glucopine
    24% identity, 77% coverage of query (53.5 bits)

5l9oA Crystal structure of agrobacterium tumefaciens c58 strain pbp soca in complex with glucopine
    24% identity, 77% coverage of query (53.5 bits)

1lafE / P02911 Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
    23% identity, 76% coverage of query (53.1 bits)

5lomB / Q7D447 Crystal structure of the pbp soca from agrobacterium tumefaciens c58 in complex with dfg at 1.5 a resolution (see paper)
    24% identity, 77% coverage of query (53.1 bits)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand
    23% identity, 76% coverage of query (53.1 bits)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein
    23% identity, 76% coverage of query (53.1 bits)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein
    23% identity, 76% coverage of query (53.1 bits)

5owfA Structure of a lao-binding protein mutant with glutamine
    23% identity, 76% coverage of query (53.1 bits)

2pvuA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus
    27% identity, 75% coverage of query (52.8 bits)

2q2aA / D0VWX8 Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
    27% identity, 75% coverage of query (52.4 bits)

2q2cA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus
    27% identity, 75% coverage of query (52.4 bits)

ArgT / b2310 lysine/arginine/ornithine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
TC 3.A.1.3.1 / TC 3.A.1.3.29 / P09551 ArgT aka B2310, component of Histidine/Arginine/Lysine (basic amino acid) uptake porter, HisJ/ArgT/HisP/HisM/HisQ [R, R, C, M, M, respectively] (Gilson et al. 1982). HisJ binds L-His (preferred), but 1-methyl-L-His and 3-methyl-L-His also bind, while the dipeptide carnosine binds weakly; D-histidine and the histidine degradation products, histamine, urocanic acid and imidazole do not bind. L-Arg, homo-L-Arg, and post-translationally modified methylated Arg-analogs also bind with the exception of symmetric dimethylated-L-Arg. L-Lys and L-Orn show weaker interactions with HisJ and methylated and acetylated Lys variants show poor binding.The carboxylate groups of these amino acids and their variants are essential from Escherichia coli (see 5 papers)
    23% identity, 76% coverage of query (51.6 bits)

TC 3.A.1.3.18 / Q8YPM9 NatF, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (see paper)
    25% identity, 75% coverage of query (51.2 bits)

1xt8B / Q0P9S0 Crystal structure of cysteine-binding protein from campylobacter jejuni at 2.0 a resolution (see paper)
    24% identity, 78% coverage of query (51.2 bits)

3k4uE / Q7MAG0 Crystal structure of putative binding component of abc transporter from wolinella succinogenes dsm 1740 complexed with lysine
    25% identity, 76% coverage of query (50.1 bits)

4ohnA / A0A0H2UKY8 Crystal structure of an abc uptake transporter substrate binding protein from streptococcus pneumoniae with bound histidine
    20% identity, 80% coverage of query (49.3 bits)

8eyzA / P0AEQ3 Engineered glutamine binding protein bound to gln and a cobaloxime ligand (see paper)
    26% identity, 73% coverage of query (47.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory