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Searching for up to 100 curated homologs for Pf1N1B4_5586 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5586 (348 a.a.)

Found high-coverage hits (≥70%) to 26 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

vanA / O05616 vanillate O-demethylase oxygenase component (EC 1.14.13.82) from Pseudomonas sp. (strain HR199 / DSM 7063) (see paper)
    79% identity, 97% coverage of query (582 bits)

Q2KQ82 vanillate monooxygenase (EC 1.14.13.82) from Comamonas testosteroni (see paper)
vanA / AAW33713.1 VanA from Comamonas testosteroni (see paper)
    75% identity, 98% coverage of query (535 bits)

Q8FRX4 vanillate monooxygenase (subunit 2/2) (EC 1.14.13.82) from Corynebacterium efficiens (see paper)
    40% identity, 97% coverage of query (254 bits)

TSAM2_COMTE / Q9AHG3 Putative toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM2; Toluenesulfonate methyl-monooxygenase oxygenase component TsaM2; EC 1.14.14.- from Comamonas testosteroni (Pseudomonas testosteroni)
    38% identity, 98% coverage of query (223 bits)

tsaM / P94679 TsaM from Comamonas testosteroni (see paper)
TSAM1_COMTE / P94679 Toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM1; TS methylmonooxygenase system, oxygenase M; Toluenesulfonate methyl-monooxygenase oxygenase component TsaM1; EC 1.14.14.- from Comamonas testosteroni (Pseudomonas testosteroni) (see paper)
    36% identity, 98% coverage of query (203 bits)

3gobA / Q5S3I3 Crystal structure of dicamba monooxygenase with non-heme cobalt and dcsa (see paper)
    36% identity, 98% coverage of query (197 bits)

DDMC_STEMA / Q5S3I3 Dicamba O-demethylase, oxygenase component; Dicamba monooxygenase; DMO; Three-component Rieske non-heme iron oxygenase system; EC 1.14.15.- from Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia) (see 5 papers)
    36% identity, 98% coverage of query (197 bits)

3gb4A Crystal structure of dicamba monooxygenase with non-heme cobalt and dicamba
    36% identity, 98% coverage of query (197 bits)

3gkeA Crystal structure of dicamba monooxygenase
    36% identity, 98% coverage of query (197 bits)

Q2KQ79 vanillate monooxygenase (EC 1.14.13.82) from Comamonas testosteroni (see paper)
ivaA / AAW33716.1 IvaA from Comamonas testosteroni (see paper)
    35% identity, 100% coverage of query (195 bits)

3gkeB Crystal structure of dicamba monooxygenase
    35% identity, 98% coverage of query (190 bits)

Q08KC3 vanillate monooxygenase (subunit 1/2) (EC 1.14.13.82) from Streptomyces sp. NL15-2K (see paper)
    34% identity, 99% coverage of query (185 bits)

6vshC / Q5S3I3 Crystal structure of apo dicamba monooxygenase (see paper)
    35% identity, 98% coverage of query (179 bits)

cndA / A0A059WLZ7 chloroacetanilide N-alkylformylase (EC 1.14.15.23) from Rhizorhabdus wittichii (strain DC-6 / KACC 16600) (see paper)
CNDA_RHIWD / A0A059WLZ7 Chloroacetanilide N-alkylformylase, oxygenase component; Three-component Rieske non-heme iron oxygenase system; RHO; EC 1.14.15.23 from Rhizorhabdus wittichii (strain DC-6 / KACC 16600) (Sphingomonas wittichii) (see paper)
A0A059WLZ7 chloroacetanilide N-alkylformylase (EC 1.14.15.23) from Rhizorhabdus wittichii (see paper)
    34% identity, 98% coverage of query (149 bits)

Build an alignment

Build an alignment for Pf1N1B4_5586 and 14 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

7qwtA Rieske non-heme iron monooxygenase for guaiacol o-demethylation
    29% identity, 97% coverage of query (111 bits)

7szeB / C3RVP5 Structure of the rieske non-heme iron oxygenase gxta with saxitoxin bound (see paper)
    25% identity, 95% coverage of query (85.1 bits)

CAO / Q9MBA1 chlorophyllide a oxygenase (EC 1.14.13.122) from Arabidopsis thaliana (see 6 papers)
CAO_ARATH / Q9MBA1 Chlorophyllide a oxygenase, chloroplastic; Chlorophyll a oxygenase; Chlorophyll b synthase; AtCAO; EC 1.14.13.122 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9MBA1 chlorophyllide-a oxygenase (EC 1.14.13.122) from Arabidopsis thaliana (see 3 papers)
    25% identity, 94% coverage of query (81.3 bits)

CAO_ORYSJ / Q8S7E1 Chlorophyllide a oxygenase, chloroplastic; Chlorophyll a oxygenase; Chlorophyll b synthase; EC 1.14.13.122 from Oryza sativa subsp. japonica (Rice)
Q8S7E1 chlorophyllide-a oxygenase (EC 1.14.13.122) from Oryza sativa Japonica Group (see paper)
    24% identity, 89% coverage of query (76.6 bits)

Q9XJ38 chlorophyllide-a oxygenase (EC 1.14.13.122) from Dunaliella salina (see paper)
    26% identity, 94% coverage of query (73.2 bits)

4qckA Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (ksha) from m. Tuberculosis in complex with 4-androstene-3,17-dione
    22% identity, 94% coverage of query (65.5 bits)

KSHA3_RHORH / F1CMX8 3-ketosteroid-9-alpha-monooxygenase, oxygenase component; 3-ketosteroid-9-alpha-hydroxylase, oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; Rieske-type oxygenase; RO; EC 1.14.15.30 from Rhodococcus rhodochrous (see paper)
F1CMX8 3-ketosteroid 9alpha-monooxygenase (EC 1.14.15.30) from Rhodococcus rhodochrous (see paper)
    23% identity, 95% coverage of query (63.9 bits)

kshA / P71875 3-ketosteroid 9α-hydroxylase oxygenase component monomer (EC 1.14.15.30) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
KSHA_MYCTU / P71875 3-ketosteroid-9-alpha-monooxygenase, oxygenase component; 3-ketosteroid-9-alpha-hydroxylase, oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; Rieske-type oxygenase; RO; EC 1.14.15.30 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
P71875 3-ketosteroid 9alpha-monooxygenase (EC 1.14.15.30) from Mycobacterium tuberculosis (see 4 papers)
    21% identity, 94% coverage of query (63.5 bits)

2zylA / P71875 Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (ksha) from m. Tuberculosis (see paper)
    22% identity, 94% coverage of query (63.5 bits)

KSHA_MYCS2 / A0R4R3 3-ketosteroid-9-alpha-monooxygenase, oxygenase component; 3-ketosteroid-9-alpha-hydroxylase, oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; Rieske-type oxygenase; RO; EC 1.14.15.30 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
Q27W16 3-ketosteroid 9alpha-monooxygenase (EC 1.14.15.30) from Mycolicibacterium smegmatis (see paper)
    22% identity, 91% coverage of query (59.7 bits)

A0A0F7JI94 3-ketosteroid 9alpha-monooxygenase (subunit 2/2) (EC 1.14.15.30) from Mycolicibacterium neoaurum (see paper)
    21% identity, 93% coverage of query (56.2 bits)

C9E9N6 3-ketosteroid 9alpha-monooxygenase (EC 1.14.15.30); steroid 9alpha-monooxygenase (EC 1.14.99.24) from Mycolicibacterium neoaurum (see 2 papers)
    21% identity, 93% coverage of query (53.9 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory