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Searching for up to 100 curated homologs for Pf1N1B4_562 Histidine transporter, periplasmic histidine-binding protein (337 a.a.)

Found high-coverage hits (≥70%) to 15 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO356_09620 ABC transporter for L-Histidine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2C3
    48% identity, 100% coverage of query (311 bits)

TC 3.A.1.12.6 / Q9KKE3 HutX aka HISX, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine from Rhizobium meliloti (Sinorhizobium meliloti) (see paper)
    45% identity, 91% coverage of query (304 bits)

Build an alignment

Build an alignment for Pf1N1B4_562 and 2 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

7yleA / A3SME7 Rndmpx in complex with dmsp (see paper)
    27% identity, 88% coverage of query (127 bits)

TC 3.A.1.12.12 / Q9HTI6 Putative uncharacterized protein, component of The CbcWV/CbcX (choline)/CaiX (carnitine)/BetX (betaine) transporter with 3 binding receptors for distinct quaternary ammonium compounds. Only the ligand-bound receptor binds to the transporter with high affinity from Pseudomonas aeruginosa (see paper)
    23% identity, 94% coverage of query (75.5 bits)

TMOX_RUEPO / Q5LT66 Trimethylamine N-oxide-binding protein; TMAO-binding protein from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) (see 2 papers)
    22% identity, 97% coverage of query (73.9 bits)

4xz6A / Q5LT66 Tmox in complex with tmao (see paper)
    24% identity, 88% coverage of query (72.0 bits)

TMOX_PELUB / Q4FL33 Trimethylamine N-oxide-binding protein; TMAO-binding protein from Pelagibacter ubique (strain HTCC1062) (see paper)
    21% identity, 95% coverage of query (65.9 bits)

TC 3.A.1.12.7 / Q8RPR1 ChoX, component of High affinity (3 μM) choline-specific uptake system from Rhizobium meliloti (Sinorhizobium meliloti) (see paper)
    24% identity, 97% coverage of query (64.3 bits)

2regA / Q92N37 Abc-transporter choline binding protein in complex with choline (see paper)
    23% identity, 91% coverage of query (60.1 bits)

2rinA Abc-transporter choline binding protein in complex with acetylcholine
    23% identity, 91% coverage of query (60.1 bits)

A0A077T7R8 ABC-type quaternary amine transporter (subunit 1/2) (EC 7.6.2.9) from Vibrio anguillarum serotype O1 (see paper)
    22% identity, 95% coverage of query (58.9 bits)

AO356_13815 ABC transporter for Carnitine, periplasmic substrate-binding component 1 from Pseudomonas fluorescens FW300-N2C3
    22% identity, 97% coverage of query (57.0 bits)

OsrA / b2679 glycine betaine ABC transporter periplasmic binding protein ProX (EC 7.6.2.9) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
proX / P0AFM2 glycine betaine ABC transporter periplasmic binding protein ProX (EC 7.6.2.9) from Escherichia coli (strain K12) (see 2 papers)
PROX_ECOLI / P0AFM2 Glycine betaine/proline betaine-binding periplasmic protein; GBBP from Escherichia coli (strain K12) (see 5 papers)
TC 3.A.1.12.1 / P0AFM2 ProX aka ProU aka B2679, component of Glycine betaine/proline porter, ProU or ProVWX (also transports proline betaine, carnitine, dimethyl proline, homobetaine, γ-butyrobetaine and choline with low affinity) from Escherichia coli (see 6 papers)
proX / RF|NP_417165 glycine betaine-binding periplasmic protein from Escherichia coli K12 (see 7 papers)
    22% identity, 70% coverage of query (52.0 bits)

1r9qA / P0AFM2 Structure analysis of prox in complex with proline betaine (see paper)
    22% identity, 70% coverage of query (51.6 bits)

TC 3.A.1.12.12 / Q9HTH6 Putative uncharacterized protein, component of The CbcWV/CbcX (choline)/CaiX (carnitine)/BetX (betaine) transporter with 3 binding receptors for distinct quaternary ammonium compounds. Only the ligand-bound receptor binds to the transporter with high affinity from Pseudomonas aeruginosa (see paper)
    20% identity, 97% coverage of query (48.5 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory