Sites on a Tree

 

Searching for up to 100 curated homologs for Pf1N1B4_5645 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5645 (281 a.a.)

Found high-coverage hits (≥70%) to 55 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO356_00480 ABC transporter for D-Glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2C3
    94% identity, 100% coverage of query (549 bits)

AO353_21710 ABC transporter for D-glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E3
    92% identity, 100% coverage of query (538 bits)

Pf6N2E2_2053 ABC transporter for D-Glucosamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    93% identity, 100% coverage of query (528 bits)

4zv2A An ancestral arginine-binding protein bound to glutamine
    34% identity, 82% coverage of query (119 bits)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
    33% identity, 82% coverage of query (117 bits)

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
    33% identity, 84% coverage of query (113 bits)

Build an alignment

Build an alignment for Pf1N1B4_5645 and 6 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

TC 3.A.1.3.16 / Q0P9X8 PEB1A, component of Uptake system for glutamate and aspartate from Campylobacter jejuni (see 2 papers)
peb1A / RF|YP_002344319.1 major cell-binding factor from Campylobacter jejuni (see 3 papers)
peb1 / AAA02919.1 major cell-binding factor from Campylobacter jejuni (see paper)
    27% identity, 94% coverage of query (104 bits)

5eyfB / Q3XZW5 Crystal structure of solute-binding protein from enterococcus faecium with bound glutamate
    31% identity, 77% coverage of query (103 bits)

6svfA / Q9WZ62 Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
    30% identity, 84% coverage of query (100 bits)

GLNH_BACSU / O34563 ABC transporter glutamine-binding protein GlnH from Bacillus subtilis (strain 168) (see paper)
    27% identity, 92% coverage of query (100 bits)

cjaA / CAJ20048.1 glutamine-binding protein from Campylobacter jejuni (see paper)
    28% identity, 90% coverage of query (97.4 bits)

1xt8B / Q0P9S0 Crystal structure of cysteine-binding protein from campylobacter jejuni at 2.0 a resolution (see paper)
    27% identity, 86% coverage of query (94.4 bits)

TcyJ / b1920 cystine ABC transporter periplasmic binding protein (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 12 papers)
tcyJ / P0AEM9 cystine ABC transporter periplasmic binding protein (EC 7.4.2.12) from Escherichia coli (strain K12) (see 13 papers)
TCYJ_ECOLI / P0AEM9 L-cystine-binding protein TcyJ; CBP; Protein FliY; Sulfate starvation-induced protein 7; SSI7 from Escherichia coli (strain K12) (see 6 papers)
TC 3.A.1.3.10 / P0AEM9 Cystine-binding periplasmic protein FLIY aka CysX aka B1920, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 7 papers)
    30% identity, 85% coverage of query (93.2 bits)

2yjpA / Q5F5B5 Crystal structure of the solute receptors for l-cysteine of neisseria gonorrhoeae (see paper)
    29% identity, 75% coverage of query (92.8 bits)

2v25A / Q0P9X8 Structure of the campylobacter jejuni antigen peb1a, an aspartate and glutamate receptor with bound aspartate (see paper)
    27% identity, 84% coverage of query (92.4 bits)

GlnH / b0811 L-glutamine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 13 papers)
GlnH / P0AEQ3 L-glutamine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli (strain K12) (see 13 papers)
GLNH_ECOLI / P0AEQ3 Glutamine-binding periplasmic protein; GlnBP from Escherichia coli (strain K12) (see paper)
TC 3.A.1.3.2 / P0AEQ3 Glutamine-binding periplasmic protein GlnH aka B0811 aka GlnBP, component of Three component ABC L-glutamine porter. The basal ATPase activity (ATP hydrolysis in the absence of substrate) is mainly caused by the docking of the closed-unliganded state of GlnH onto the transporter domain of GlnPQ. Unlike glutamine, arginine binds both GlnH domains, but does not trigger their closing. Comparison of the ATPase activity in nanodiscs with glutamine transport in proteoliposomes suggested that the stoichiometry of ATP per substrate is close to two from Escherichia coli (see 7 papers)
glnH / GB|AAN79369.1 glutamine ABC transporter, periplasmic glutamine-binding protein GlnH from Escherichia coli (see 7 papers)
    28% identity, 83% coverage of query (89.7 bits)

TC 3.A.1.3.18 / Q8YPM9 NatF, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (see paper)
    28% identity, 84% coverage of query (88.6 bits)

8eyzA / P0AEQ3 Engineered glutamine binding protein bound to gln and a cobaloxime ligand (see paper)
    27% identity, 83% coverage of query (84.7 bits)

2y7iA / Q8ZKA9 Structural basis for high arginine specificity in salmonella typhimurium periplasmic binding protein stm4351. (see paper)
    29% identity, 83% coverage of query (80.5 bits)

3k4uE / Q7MAG0 Crystal structure of putative binding component of abc transporter from wolinella succinogenes dsm 1740 complexed with lysine
    28% identity, 81% coverage of query (80.1 bits)

TC 3.A.1.3.12 / P73544 BgtB aka GLNH aka SLL1270, component of Arginine/lysine/histidine/glutamine porter (see paper)
    27% identity, 83% coverage of query (78.2 bits)

5kkwA / Q4FLR5 Crystal structure of sar11_1068 bound to a sulfobetaine (3-(1- methylpiperidinium-1-yl)propane-1-sulfonate)
    30% identity, 77% coverage of query (77.0 bits)

4h5fA Crystal structure of an amino acid abc transporter substrate-binding protein from streptococcus pneumoniae canada mdr_19a bound to l- arginine, form 1
    31% identity, 73% coverage of query (76.3 bits)

Ac3H11_2555 ABC transporter for L-Histidine, periplasmic substrate-binding component 1 from Acidovorax sp. GW101-3H11
    29% identity, 80% coverage of query (75.9 bits)

6h2tA / P96257 Glnh bound to glu, mycobacterium tuberculosis (see paper)
    27% identity, 88% coverage of query (75.5 bits)

6h20A Glnh bound to asn, mycobacterium tuberculosis
    27% identity, 88% coverage of query (75.5 bits)

6h1uA Glnh bound to asp, mycobacterium tuberculosis
    27% identity, 88% coverage of query (75.5 bits)

ArtJ / b0860 L-arginine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see paper)
ArtJ / P30860 L-arginine ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli (strain K12) (see paper)
ARTJ_ECOLI / P30860 ABC transporter arginine-binding protein 1 from Escherichia coli (strain K12) (see paper)
TC 3.A.1.3.3 / P30860 ArtJ aka B0860, component of Arginine porter from Escherichia coli (see 7 papers)
artJ / GB|BAA35574.1 arginine ABC transporter, periplasmic arginine-binding protein ArtJ from Escherichia coli K12 (see 8 papers)
    26% identity, 82% coverage of query (73.6 bits)

SMc02118 ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, periplasmic substrate-binding component from Sinorhizobium meliloti 1021
    27% identity, 94% coverage of query (72.4 bits)

TC 3.A.1.3.17 / Q8YSA2 Basic amino acid uptake transporter, BgtAB (see paper)
    26% identity, 84% coverage of query (71.2 bits)

AO356_09900 ABC transporter for L-Lysine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2C3
    27% identity, 84% coverage of query (70.9 bits)

4ymxA / Q8RCC4 Crystal structure of the substrate binding protein of an amino acid abc transporter (see paper)
    27% identity, 81% coverage of query (69.3 bits)

AAPJ_RHIJ3 / Q52812 General L-amino acid-binding periplasmic protein AapJ from Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841) (Rhizobium leguminosarum bv. viciae) (see 2 papers)
TC 3.A.1.3.8 / Q52812 AapJ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) from Rhizobium leguminosarum (biovar viciae) (see 2 papers)
aapJ / CAA57933.1 general amino acid ABC type transporter from Rhizobium leguminosarum (see paper)
    25% identity, 86% coverage of query (68.9 bits)

Pf6N2E2_5402 ABC transporter for D-Alanine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E2
    26% identity, 84% coverage of query (68.2 bits)

2ia4B / A0A0H2UXX1 Crystal structure of novel amino acid binding protein from shigella flexneri
    24% identity, 92% coverage of query (67.0 bits)

4zefA / Q837S0 Crystal structure of substrate binding domain 2 (sbd2) of abc transporter glnpq from enterococcus faecalis
    26% identity, 75% coverage of query (66.2 bits)

2vhaA Debp
    24% identity, 91% coverage of query (65.5 bits)

4z9nB / A0A0M3KL33 Abc transporter / periplasmic binding protein from brucella ovis with glutathione bound
    26% identity, 85% coverage of query (64.3 bits)

ARTJ_CHLPN / Q9Z869 Probable ABC transporter arginine-binding protein ArtJ from Chlamydia pneumoniae (Chlamydophila pneumoniae) (see 2 papers)
    28% identity, 77% coverage of query (62.8 bits)

2q2aA / D0VWX8 Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
    25% identity, 84% coverage of query (60.5 bits)

2pvuA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus
    25% identity, 82% coverage of query (60.1 bits)

8b5dA Exploring the ligand binding and conformational dynamics of receptor domain 1 of the abc transporter glnpq
    27% identity, 70% coverage of query (59.7 bits)

2q2cA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus
    25% identity, 81% coverage of query (59.7 bits)

3vvfA Crystal structure of ttc0807 complexed with arginine
    25% identity, 88% coverage of query (58.9 bits)

3vveA Crystal structure of ttc0807 complexed with lysine
    25% identity, 88% coverage of query (58.9 bits)

3vvdA / Q72JG5 Crystal structure of ttc0807 complexed with ornithine (see paper)
    25% identity, 88% coverage of query (58.9 bits)

3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec)
    25% identity, 85% coverage of query (58.5 bits)

6fxgB / Q9CES5 Crystal structure of substrate binding domain 1 (sbd1) of abc transporter glnpq in complex with asparagine
    27% identity, 70% coverage of query (58.2 bits)

8b5eA Exploring the ligand binding and conformational dynamics of receptor domain 1 of the abc transporter glnpq
    27% identity, 70% coverage of query (58.2 bits)

8ovoA / P37902,P42212 X-ray structure of the sf-iglusnfr-s72a in complex with l-aspartate
    24% identity, 85% coverage of query (58.2 bits)

5lomB / Q7D447 Crystal structure of the pbp soca from agrobacterium tumefaciens c58 in complex with dfg at 1.5 a resolution (see paper)
    25% identity, 81% coverage of query (57.4 bits)

5l9oA Crystal structure of agrobacterium tumefaciens c58 strain pbp soca in complex with glucopine
    25% identity, 81% coverage of query (57.4 bits)

5l9oB Crystal structure of agrobacterium tumefaciens c58 strain pbp soca in complex with glucopine
    25% identity, 81% coverage of query (57.4 bits)

6detA / S3MDF0 The crystal structure of tv2483 bound to l-arginine (see paper)
    23% identity, 89% coverage of query (55.1 bits)

4i62A / A0A0H2ZN67 1.05 angstrom crystal structure of an amino acid abc transporter substrate-binding protein abpa from streptococcus pneumoniae canada mdr_19a bound to l-arginine
    22% identity, 77% coverage of query (50.4 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory