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Searching for up to 100 curated homologs for Pf6N2E2_2924 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2924 (418 a.a.)

Found high-coverage hits (≥70%) to 11 curated proteins.

Removed hits that are identical to the query, leaving 10

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

TC 3.A.1.4.8 / P21628 High-affinity branched-chain amino acid transport system permease protein BraE, component of Branched chain amino acid uptake transporter. Transports alanine from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    76% identity, 100% coverage of query (572 bits)

LivM / b3456 branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM (EC 7.4.2.2) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
LivM / P22729 branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM (EC 7.4.2.2) from Escherichia coli (strain K12) (see 5 papers)
LIVM_ECOLI / P22729 High-affinity branched-chain amino acid transport system permease protein LivM; LIV-I protein M from Escherichia coli (strain K12) (see paper)
TC 3.A.1.4.1 / P22729 LivM aka B3456, component of Leucine; leucine/isoleucine/valine porter from Escherichia coli (see 5 papers)
    61% identity, 99% coverage of query (454 bits)

AZOBR_RS08240 L-proline and D-alanine ABC transporter, permease component 2 from Azospirillum brasilense Sp245
    64% identity, 77% coverage of query (407 bits)

braE / CAB75550.1 integral membrane protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    60% identity, 72% coverage of query (340 bits)

HSERO_RS00890 L-proline ABC transporter, permease component 2 from Herbaspirillum seropedicae SmR1
    42% identity, 74% coverage of query (249 bits)

Ac3H11_1694 ABC transporter for glutamine/isoleucine/leucine/phenylalanine/proline/serine/tyrosine, permease component 2 from Acidovorax sp. GW101-3H11
    45% identity, 77% coverage of query (218 bits)

TC 3.A.1.4.10 / Q8DQH9 ABC transporter membrane-spanning permease-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM from Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
    38% identity, 70% coverage of query (157 bits)

TC 3.A.1.4.3 / Q8VM84 BraE aka Bra2E, component of General L- (and D-)amino acid uptake porter (transports acidic, basic, polar, semipolar and hydrophobic amino acids). The amino and carboxyl groups do not need to be α since γ-aminobutyric acid (GABA) is a substrate. The system may function with additional binding proteins since L-alanine uptake is not dependent on BraC from Rhizobium leguminosarum (biovar viciae) (see paper)
    32% identity, 73% coverage of query (139 bits)

Build an alignment

Build an alignment for Pf6N2E2_2924 and 8 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Additional hits (identity < 30%)

braD / CAB75549.1 integral membrane protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    25% identity, 71% coverage of query (52.4 bits)

AZOBR_RS08235 L-proline and D-alanine ABC transporter, permease component 1 from Azospirillum brasilense Sp245
    25% identity, 70% coverage of query (52.0 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory