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Searching for up to 100 curated homologs for Pf6N2E2_3172 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3172 (264 a.a.)

Found high-coverage hits (≥70%) to 59 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

YafV / b0219 2-oxoglutaramate amidase (EC 3.5.1.3; EC 3.5.1.111) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
yafV / Q47679 2-oxoglutaramate amidase (EC 3.5.1.3) from Escherichia coli (strain K12) (see paper)
    51% identity, 97% coverage of query (248 bits)

YAFV_YEREN / P0DP67 Omega-amidase YafV; yeYafV; EC 3.5.1.3 from Yersinia enterocolitica (see paper)
    51% identity, 96% coverage of query (248 bits)

YAFV_ECOBD / A0A140NDS5 Omega-amidase YafV; ecYafV; EC 3.5.1.3 from Escherichia coli (strain B / BL21-DE3) (see paper)
    51% identity, 97% coverage of query (248 bits)

D2SGH7 nitrilase (EC 3.5.5.1) from Geodermatophilus obscurus (see paper)
    41% identity, 95% coverage of query (179 bits)

A3DHT2 nitrilase (EC 3.5.5.1) from Acetivibrio thermocellus (see paper)
    36% identity, 92% coverage of query (148 bits)

mtnU / O31664 2-oxoglutaramate:glutamine aminotransferase (EC 3.5.1.3) from Bacillus subtilis (strain 168) (see 4 papers)
MTNU_BACSU / O31664 2-oxoglutaramate amidase; EC 3.5.1.111 from Bacillus subtilis (strain 168) (see 2 papers)
    32% identity, 92% coverage of query (133 bits)

YAFV_STAA4 / P0DP65 Omega-amidase YafV; saYafV; EC 3.5.1.3 from Staphylococcus aureus (strain 04-02981) (see paper)
    30% identity, 92% coverage of query (105 bits)

AO353_07425 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2E3
    33% identity, 83% coverage of query (100 bits)

Pf1N1B4_2504 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N1B4
    33% identity, 83% coverage of query (100 bits)

PfGW456L13_1170 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens GW456-L13
    34% identity, 83% coverage of query (97.8 bits)

Pf6N2E2_4777 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2E2
    33% identity, 83% coverage of query (89.7 bits)

AO356_14225 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2C3
    33% identity, 83% coverage of query (88.6 bits)

B3IVI7 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (see 4 papers)
    30% identity, 92% coverage of query (85.1 bits)

ramA / Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
RSAM_PSESP / Q75SP7 (R)-stereoselective amidase; EC 3.5.1.100 from Pseudomonas sp. (see paper)
Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
    30% identity, 91% coverage of query (84.0 bits)

Q9HVU6 deaminated glutathione amidase (EC 3.5.1.128) from Pseudomonas aeruginosa (see paper)
    31% identity, 92% coverage of query (83.2 bits)

Build an alignment

Build an alignment for Pf6N2E2_3172 and 15 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

F8AEP0 amidase (EC 3.5.1.4) from Pyrococcus yayanosii (see paper)
    29% identity, 84% coverage of query (82.8 bits)

ORF77 / Q93NG1 2-ketoglutaramate amidase monomer (EC 3.5.1.3) from Paenarthrobacter nicotinovorans (see 2 papers)
NIT_PAENI / Q93NG1 2-oxoglutaramate amidase; Omega-amidase; EC 3.5.1.111 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
Q93NG1 2-oxoglutaramate amidase (EC 3.5.1.111) from Paenarthrobacter nicotinovorans (see paper)
    31% identity, 92% coverage of query (81.6 bits)

davA / Q88QV2 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
Q88QV2 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas putida (see paper)
    29% identity, 92% coverage of query (81.3 bits)

NIT3_YEAST / P49954 Omega-amidase NIT3; Nitrilase homolog 2; EC 3.5.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
    25% identity, 92% coverage of query (81.3 bits)

YCU9_SCHPO / O59829 Probable nitrilase C965.09; EC 3.5.-.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    27% identity, 91% coverage of query (80.1 bits)

NILP3_ARATH / Q8RUF8 Omega-amidase, chloroplastic; Nitrilase-like protein 3; EC 3.5.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q8RUF8 omega-amidase (EC 3.5.1.3) from Arabidopsis thaliana (see paper)
    28% identity, 93% coverage of query (76.6 bits)

Q8PXI9 nitrilase (EC 3.5.5.1) from Methanosarcina mazei (see paper)
    28% identity, 84% coverage of query (75.9 bits)

SGR_6069 / B1W481 4-guanidinobutanamidase (EC 3.5.1.4) from Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) (see 2 papers)
    33% identity, 79% coverage of query (75.5 bits)

3klcA / Q9UYV8 Crystal structure of hyperthermophilic nitrilase (see paper)
    27% identity, 91% coverage of query (74.3 bits)

3klcB Crystal structure of hyperthermophilic nitrilase
    27% identity, 91% coverage of query (74.3 bits)

NIT2_HUMAN / Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 4 papers)
Q9NQR4 omega-amidase (EC 3.5.1.3) from Homo sapiens (see paper)
    27% identity, 91% coverage of query (73.6 bits)

NITR_PYRAB / Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see 2 papers)
    27% identity, 84% coverage of query (73.6 bits)

Nit2 / Q9JHW2 ω-amidase NIT2 monomer (EC 3.5.1.3) from Mus musculus (see 2 papers)
NIT2_MOUSE / Q9JHW2 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Mus musculus (Mouse) (see 3 papers)
    27% identity, 91% coverage of query (72.8 bits)

NIT2_RAT / Q497B0 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Rattus norvegicus (Rat) (see 2 papers)
    26% identity, 91% coverage of query (72.4 bits)

NIT1_YEREN / P0DP68 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; yeNit1; EC 3.5.1.128 from Yersinia enterocolitica (see paper)
P0DP68 deaminated glutathione amidase (EC 3.5.1.128) from Yersinia enterocolitica (see paper)
    27% identity, 96% coverage of query (70.5 bits)

6ypaB / O58376 The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
    26% identity, 88% coverage of query (70.5 bits)

7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide
    26% identity, 88% coverage of query (70.5 bits)

NIT1_SYNYG / P0DP66 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; syNit1; EC 3.5.1.128 from Synechocystis sp. (strain PCC 6803 / GT-S) (see paper)
P0DP66 deaminated glutathione amidase (EC 3.5.1.128) from Synechocystis sp. PCC 6803 (see paper)
    29% identity, 86% coverage of query (69.7 bits)

A0A088BHP3 aryl-acylamidase (EC 3.5.1.13); amidase (EC 3.5.1.4) from Streptomyces sp. (see paper)
    30% identity, 79% coverage of query (69.3 bits)

NILP2_ARATH / Q94JV5 Deaminated glutathione amidase, chloroplastic/cytosolic; dGSH amidase; Nitrilase-like protein 2; Protein nitrilase 1 homolog; AtNit1; Protein Nit1 homolog; EC 3.5.1.128 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q94JV5 deaminated glutathione amidase (EC 3.5.1.128) from Arabidopsis thaliana (see paper)
    26% identity, 92% coverage of query (68.2 bits)

A0QQY9 D-inositol-3-phosphate glycosyltransferase (EC 2.4.1.250) from Mycolicibacterium smegmatis (see paper)
    30% identity, 83% coverage of query (66.6 bits)

A0A0E3D8K7 nitrilase (EC 3.5.5.1); Aliphatic nitrilase (EC 3.5.5.7) from Fusarium proliferatum (see paper)
    30% identity, 80% coverage of query (65.9 bits)

pydC / Q846U4 3-amino-isobutyrate synthase (EC 3.5.1.6) from Brevibacillus agri (see paper)
    28% identity, 80% coverage of query (65.5 bits)

B8C1M9 nitrilase (EC 3.5.5.1) from Thalassiosira pseudonana (see paper)
    24% identity, 91% coverage of query (64.7 bits)

4iztA / D0VWZ1 The e41q mutant of the amidase from nesterenkonia sp. An1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine
    28% identity, 89% coverage of query (62.8 bits)

C6K3Z5 amidase (EC 3.5.1.4) from Nesterenkonia sp. (see paper)
    28% identity, 87% coverage of query (61.6 bits)

4izuA The e41q mutant of the amidase from nesterenkonia sp. An1 showing the result of michael addition of acrylamide at the active site cysteine
    28% identity, 88% coverage of query (61.2 bits)

aguB / Q9I6J8 N-carbamoylputrascine amidohydrolase subunit (EC 3.5.1.53) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    27% identity, 82% coverage of query (60.8 bits)

5nybA A c145a mutant of nesterenkonia an1 amidase bound to adipamide
    28% identity, 89% coverage of query (59.7 bits)

5ny7A A c145a mutant of nesterenkonia an1 amidase bound to nicotinamide
    28% identity, 89% coverage of query (59.7 bits)

B8A0E5 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    28% identity, 83% coverage of query (59.3 bits)

B8A2V8 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    27% identity, 83% coverage of query (58.9 bits)

5nycA A c145a mutant of nesterenkonia an1 amidase bound to propionitrile
    28% identity, 88% coverage of query (58.5 bits)

4izsA The c145a mutant of the amidase from nesterenkonia sp. An1 in complex with butyramide
    28% identity, 88% coverage of query (58.5 bits)

NIT1_MOUSE / Q8VDK1 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Mus musculus (Mouse) (see 7 papers)
Q8VDK1 deaminated glutathione amidase (EC 3.5.1.128) from Mus musculus (see paper)
    28% identity, 88% coverage of query (56.2 bits)

NFT1_CAEEL / O76463 Nitrilase and fragile histidine triad fusion protein NitFhit; EC 3.6.1.29; EC 3.5.-.- from Caenorhabditis elegans (see 2 papers)
    27% identity, 90% coverage of query (55.8 bits)

B0V8W9 NAD+ synthase (glutamine-hydrolysing) (EC 6.3.5.1) from Acinetobacter baumannii (see paper)
    26% identity, 75% coverage of query (55.8 bits)

5khaA / V5VHL3 Structure of glutamine-dependent NAD+ synthetase from acinetobacter baumannii in complex with adenosine diphosphate (adp)
    26% identity, 75% coverage of query (55.5 bits)

AMIE_HELPY / O25067 Aliphatic amidase; Acylamide amidohydrolase; EC 3.5.1.4 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see 3 papers)
    26% identity, 87% coverage of query (55.5 bits)

NADE_ACIAD / Q6F8K4 Glutamine-dependent NAD(+) synthetase; NAD synthetase; NAD(+) synthase [glutamine-hydrolyzing]; EC 6.3.5.1 from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
    26% identity, 73% coverage of query (55.5 bits)

NIT1_HUMAN / Q86X76 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Homo sapiens (Human) (see paper)
    28% identity, 90% coverage of query (53.1 bits)

AMIE_RHOER / Q01360 Aliphatic amidase; Acylamide amidohydrolase; Wide spectrum amidase; EC 3.5.1.4 from Rhodococcus erythropolis (Arthrobacter picolinophilus)
    26% identity, 87% coverage of query (51.6 bits)

NFT1_DROME / O76464 Nitrilase and fragile histidine triad fusion protein NitFhit; NFT-1 protein; EC 3.6.1.29; EC 3.5.-.- from Drosophila melanogaster (Fruit fly) (see paper)
    29% identity, 80% coverage of query (50.1 bits)

P59701 formamidase (EC 3.5.1.49) from Bacillus cereus (see paper)
    24% identity, 82% coverage of query (47.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory