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Searching for up to 100 curated homologs for Pf6N2E2_5686 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5686 (447 a.a.)

Found high-coverage hits (≥70%) to 60 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

BPHYT_RS23160 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) from Burkholderia phytofirmans PsJN
    41% identity, 96% coverage of query (354 bits)

SO1268 Glutamate--putrescine ligase (EC 6.3.1.11) from Shewanella oneidensis MR-1
    41% identity, 96% coverage of query (338 bits)

PS417_27140 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) from Pseudomonas simiae WCS417
    32% identity, 94% coverage of query (219 bits)

Psest_4288 Glutamate--putrescine ligase (EC 6.3.1.11) from Pseudomonas stutzeri RCH2
    32% identity, 95% coverage of query (218 bits)

AO353_08595 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) from Pseudomonas fluorescens FW300-N2E3
    32% identity, 94% coverage of query (215 bits)

PfGW456L13_925 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) from Pseudomonas fluorescens GW456-L13
    32% identity, 94% coverage of query (215 bits)

AO356_13135 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) from Pseudomonas fluorescens FW300-N2C3
    32% identity, 94% coverage of query (215 bits)

Pf1N1B4_2254 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) from Pseudomonas fluorescens FW300-N1B4
    32% identity, 93% coverage of query (215 bits)

ipuC / Q936T0 γ-glutamylisopropylamide synthetase from Pseudomonas sp. (see paper)
IPUC_PSESP / Q936T0 Glutamate--isopropylamine ligase; Gamma-glutamylisopropylamide synthetase; GIPA synthetase; EC 6.3.2.- from Pseudomonas sp. (see paper)
    33% identity, 95% coverage of query (207 bits)

Pf6N2E2_4509 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) from Pseudomonas fluorescens FW300-N2E2
    33% identity, 83% coverage of query (206 bits)

GLNA4_STRCO / O88070 Gamma-glutamylethanolamide synthetase GlnA4; EC 6.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 4 papers)
    33% identity, 95% coverage of query (199 bits)

P36687 glutamine synthetase (EC 6.3.1.2) from Pyrococcus woesei (see paper)
    30% identity, 97% coverage of query (184 bits)

O33342 glutamine synthetase (EC 6.3.1.2) from Mycobacterium tuberculosis (see paper)
    33% identity, 83% coverage of query (182 bits)

GLNA_THEKO / O08467 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
O08467 glutamine synthetase (EC 6.3.1.2) from Pyrococcus sp. (see paper)
    33% identity, 84% coverage of query (181 bits)

BWI76_RS10710 Glutamate--putrescine ligase (EC 6.3.1.11) from Klebsiella michiganensis M5al
    31% identity, 98% coverage of query (173 bits)

YcjK / b1297 glutamate-putrescine ligase (EC 6.3.1.11) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
puuA / P78061 glutamate-putrescine ligase (EC 6.3.1.11) from Escherichia coli (strain K12) (see 3 papers)
PUUA_ECOLI / P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see 3 papers)
puuA / BAD88705.1 gamma-glutamylputrescine synthetase from Escherichia coli K-12 (see paper)
    31% identity, 98% coverage of query (171 bits)

8tfkA Glutamine synthetase
    31% identity, 83% coverage of query (166 bits)

GLNA1_METMA / Q8PY99 Glutamine synthetase; GS; Glutamate--ammonia ligase; EC 6.3.1.2 from Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) (see paper)
Q8PY99 glutamine synthetase (EC 6.3.1.2) from Methanosarcina mazei (see paper)
    31% identity, 83% coverage of query (165 bits)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
    31% identity, 83% coverage of query (165 bits)

GLN1A_BACSU / P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 9 papers)
P12425 glutamine synthetase (EC 6.3.1.2) from Bacillus subtilis (see 2 papers)
    30% identity, 89% coverage of query (164 bits)

4s0rD / P12425 Structure of gs-tnra complex (see paper)
    30% identity, 89% coverage of query (164 bits)

8ufjB / Q8PY99 8ufjB (see paper)
    31% identity, 83% coverage of query (164 bits)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex
    30% identity, 89% coverage of query (164 bits)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex
    30% identity, 89% coverage of query (164 bits)

Q0E5H7 glutamine synthetase (EC 6.3.1.2) from Halobacillus halophilus (see paper)
    30% identity, 84% coverage of query (159 bits)

Build an alignment

Build an alignment for Pf6N2E2_5686 and 25 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
    29% identity, 93% coverage of query (159 bits)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus
    29% identity, 93% coverage of query (159 bits)

GLNA3_HALMT / I3R176 Glutamine synthetase 3; GS3; Glutamate--ammonia ligase; EC 6.3.1.2 from Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) (see paper)
B8ZJH0 glutamine synthetase (EC 6.3.1.2) from Haloferax mediterranei (see paper)
    30% identity, 83% coverage of query (158 bits)

3ng0A / P77961 Crystal structure of glutamine synthetase from synechocystis sp. Pcc 6803
    32% identity, 79% coverage of query (158 bits)

glnA / CAA78366.1 glutamine synthetase from Synechococcus sp. PCC 7002 (see paper)
    32% identity, 79% coverage of query (157 bits)

8ooxB / A0A832VZP6 8ooxB (see paper)
    29% identity, 84% coverage of query (157 bits)

8oozA Glutamine synthetase
    30% identity, 85% coverage of query (157 bits)

5dm3C / Q1QZR8 Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
    33% identity, 79% coverage of query (156 bits)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom
    30% identity, 88% coverage of query (156 bits)

7tfaB / A0A0F0G8G2 7tfaB (see paper)
    30% identity, 88% coverage of query (155 bits)

GLNA_ARCFU / O29313 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
O29313 glutamine synthetase (EC 6.3.1.2) from Archaeoglobus fulgidus (see paper)
    28% identity, 95% coverage of query (154 bits)

7tf9S / A0A5D5GA79 L. Monocytogenes gs(14)-q-glnr peptide (see paper)
    29% identity, 88% coverage of query (153 bits)

7tenA Glutamine synthetase
    29% identity, 88% coverage of query (153 bits)

GLNA_METJA / Q60182 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q60182 glutamine synthetase (EC 6.3.1.2) from Methanocaldococcus jannaschii (see paper)
    29% identity, 93% coverage of query (151 bits)

GLN1B_SYNY3 / P77961 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see 3 papers)
P77961 glutamine synthetase (EC 6.3.1.2) from Synechocystis sp. (see paper)
    31% identity, 79% coverage of query (150 bits)

Q9RHZ1 glutamine synthetase (EC 6.3.1.2) from Trichormus azollae (see paper)
    31% identity, 79% coverage of query (150 bits)

P64246 glutamine synthetase (EC 6.3.1.2) from Mycobacterium tuberculosis variant bovis (see paper)
    30% identity, 81% coverage of query (149 bits)

GLN1B_NOSS1 / P00964 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
    30% identity, 79% coverage of query (149 bits)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    30% identity, 81% coverage of query (149 bits)

P36205 glutamine synthetase (EC 6.3.1.2) from Thermotoga maritima (see paper)
    27% identity, 93% coverage of query (148 bits)

GLN1B_STRCO / P15106 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
P15106 glutamine synthetase (EC 6.3.1.2) from Streptomyces coelicolor (see paper)
    33% identity, 76% coverage of query (148 bits)

5dm3A / Q1QZR8 Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
    31% identity, 83% coverage of query (148 bits)

GLNA2_STRCO / Q9RDS6 Gamma-glutamylpolyamine synthetase GlnA2; EC 6.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 3 papers)
    30% identity, 83% coverage of query (147 bits)

7tf6A Glutamine synthetase
    28% identity, 89% coverage of query (146 bits)

7tdvC / E3VXC2 7tdvC (see paper)
    28% identity, 89% coverage of query (145 bits)

7cquA Gmas/adp/metsox-p complex
    30% identity, 96% coverage of query (135 bits)

7cqqA Gmas in complex with amppnp and metsox
    30% identity, 96% coverage of query (135 bits)

7cqnA Gmas in complex with amppcp
    30% identity, 96% coverage of query (135 bits)

7cqwA / A0A369R1N0 Gmas/adp complex-conformation 1 (see paper)
    30% identity, 96% coverage of query (135 bits)

8wwuB / A0A7Y1Q2L1 8wwuB
    26% identity, 99% coverage of query (129 bits)

8wwvA Glutamine synthetase
    26% identity, 99% coverage of query (129 bits)

5zlpJ / P94845 Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
    28% identity, 84% coverage of query (124 bits)

5zlpL Crystal structure of glutamine synthetase from helicobacter pylori
    28% identity, 84% coverage of query (124 bits)

5zlpA Crystal structure of glutamine synthetase from helicobacter pylori
    28% identity, 84% coverage of query (124 bits)

4hppA / Q9HT65 Crystal structure of novel glutamine synthase homolog (see paper)
    30% identity, 94% coverage of query (95.9 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory