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Searching for up to 100 curated homologs for Pf6N2E2_6062 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_6062 (375 a.a.)

Found high-coverage hits (≥70%) to 92 curated proteins.

Removed hits that are identical to the query, leaving 91

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Q9I5E3 2-methylcitrate synthase (EC 2.3.3.5) from Pseudomonas aeruginosa (see paper)
    87% identity, 100% coverage of query (681 bits)

6s87D / Q9I5E3 Crystal structure of 2-methylcitrate synthase (prpc) from pseudomonas aeruginosa in complex with oxaloacetate.
    87% identity, 97% coverage of query (662 bits)

SO0344 2-methylcitrate synthase (EC 2.3.3.5) from Shewanella oneidensis MR-1
PRPC_SHEON / Q8EJW2 2-methylcitrate synthase; 2-MCS; MCS; Citrate synthase; EC 2.3.3.5; EC 2.3.3.16 from Shewanella oneidensis (strain ATCC 700550 / JCM 31522 / CIP 106686 / LMG 19005 / NCIMB 14063 / MR-1) (see paper)
    74% identity, 100% coverage of query (586 bits)

Q9KSC1 2-methylcitrate synthase (EC 2.3.3.5) from Vibrio cholerae (see paper)
    69% identity, 100% coverage of query (537 bits)

Q2Z1A8 2-methylcitrate synthase (EC 2.3.3.5) from Cupriavidus necator (see paper)
    53% identity, 100% coverage of query (430 bits)

PRPC_CUPNE / Q937N9 2-methylcitrate synthase; 2-MCS; MCS; Citrate synthase; EC 2.3.3.5; EC 2.3.3.16 from Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha) (see paper)
Q937N9 2-methylcitrate synthase (EC 2.3.3.5) from Cupriavidus metallidurans (see 2 papers)
    53% identity, 100% coverage of query (425 bits)

prpC / Q56063 2-methylcitrate synthase (EC 2.3.3.5) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
PRPC_SALTY / Q56063 2-methylcitrate synthase; 2-MCS; mcs; Citrate synthase; EC 2.3.3.5; EC 2.3.3.16 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
Q56063 2-methylcitrate synthase (EC 2.3.3.5) from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
    53% identity, 97% coverage of query (407 bits)

Q8X694 2-methylcitrate synthase (EC 2.3.3.5) from Escherichia coli (see paper)
    53% identity, 97% coverage of query (404 bits)

YahS / b0333 2-methylcitrate synthase (EC 2.3.3.5) from Escherichia coli K-12 substr. MG1655 (see 11 papers)
prpC / P31660 2-methylcitrate synthase (EC 2.3.3.5) from Escherichia coli (strain K12) (see 9 papers)
PRPC_ECOLI / P31660 2-methylcitrate synthase; 2-MCS; MCS; (2S,3S)-2-methylcitrate synthase; Citrate synthase; EC 2.3.3.5; EC 2.3.3.16 from Escherichia coli (strain K12) (see 7 papers)
P31660 2-methylcitrate synthase (EC 2.3.3.5) from Escherichia coli (see 4 papers)
prpC / GB|AP_000985.1 2-methylcitrate synthase; EC 2.3.3.5 from Escherichia coli K12 (see 4 papers)
    53% identity, 97% coverage of query (404 bits)

P39120 Citrate synthase 2; Citrate synthase II; EC 2.3.3.16 from Bacillus subtilis (strain 168)
    44% identity, 97% coverage of query (308 bits)

CISY_HALVD / D4GS06 Citrate synthase; EC 2.3.3.16 from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
D4GS06 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Haloferax volcanii (see paper)
    39% identity, 97% coverage of query (283 bits)

Q974S5 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Sulfurisphaera tokodaii (see paper)
    38% identity, 97% coverage of query (280 bits)

ctsA / AAA52231.1 citrate synthase from Bacillus coagulans (see paper)
    41% identity, 94% coverage of query (277 bits)

PRPC1_CORGL / Q8NSH7 2-methylcitrate synthase 1; 2-MCS; MCS; Citrate synthase 1; CS; EC 2.3.3.5; EC 2.3.3.16 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
Q8NSH7 2-methylcitrate synthase (EC 2.3.3.5) from Corynebacterium glutamicum (see paper)
    40% identity, 97% coverage of query (273 bits)

Q9R6Y4 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Nostoc sp. PCC 7120 = FACHB-418 (see paper)
    38% identity, 97% coverage of query (272 bits)

Q53554 citrate (Si)-synthase (EC 2.3.3.1) from Pyrococcus furiosus (see paper)
    38% identity, 97% coverage of query (271 bits)

PRPC2_CORGL / Q8NSL1 2-methylcitrate synthase 2; 2-MCS; MCS; Citrate synthase 2; CS; EC 2.3.3.5; EC 2.3.3.16 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
Q8NSL1 2-methylcitrate synthase (EC 2.3.3.5) from Corynebacterium glutamicum (see paper)
    41% identity, 97% coverage of query (271 bits)

1aj8A / Q53554 Citrate synthase from pyrococcus furiosus (see paper)
    38% identity, 97% coverage of query (271 bits)

PRPC_ABDS2 / O34002 2-methylcitrate synthase; 2-MCS; MCS; Citrate synthase; EC 2.3.3.5; EC 2.3.3.16 from Antarctic bacterium DS2-3R (see 3 papers)
O34002 citrate (Si)-synthase (EC 2.3.3.1) from antarctic bacterium DS2-3R (see paper)
    41% identity, 96% coverage of query (270 bits)

1a59A / O34002 Cold-active citrate synthase (see paper)
    41% identity, 96% coverage of query (270 bits)

PRPC_MYCTE / H8F0D7 2-methylcitrate synthase; 2-MCS; MCS; Citrate synthase; CS; EC 2.3.3.5; EC 2.3.3.16 from Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman) (see paper)
PRPC_MYCTU / I6Y9Q3 2-methylcitrate synthase; 2-MCS; MCS; Citrate synthase; EC 2.3.3.5; EC 2.3.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
    42% identity, 95% coverage of query (268 bits)

citZ / Q5UZI9 citrate synthase (EC 2.3.3.3) from Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (see paper)
    37% identity, 97% coverage of query (264 bits)

Q8ZWP2 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Pyrobaculum aerophilum (see paper)
    38% identity, 97% coverage of query (263 bits)

1ixeA Crystal structure of citrate synthase from thermus thermophilus hb8
    37% identity, 97% coverage of query (260 bits)

Q5SIM6 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Thermus thermophilus (see paper)
    37% identity, 97% coverage of query (259 bits)

1iomA / Q5SIM6 Crystal structure of citrate synthase from thermus thermophilus hb8 (see paper)
    37% identity, 97% coverage of query (259 bits)

MMGD_BACSU / P45858 Citrate/2-methylcitrate synthase; 2-methylcitrate synthase; 2-MCS; MCS; Citrate synthase; EC 2.3.3.-; EC 2.3.3.16 from Bacillus subtilis (strain 168) (see 2 papers)
P45858 citrate synthase (unknown stereospecificity) (EC 2.3.3.16); 2-methylcitrate synthase (EC 2.3.3.5) from Bacillus subtilis (see paper)
    39% identity, 96% coverage of query (259 bits)

A8YCJ7 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Microcystis aeruginosa (see paper)
    38% identity, 97% coverage of query (254 bits)

Q59977 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Synechocystis sp. PCC 6803 (see paper)
    36% identity, 97% coverage of query (251 bits)

6abyA / A4YGX6 Crystal structure of citrate synthase (msed_1522) from metallosphaera sedula in complex with oxaloacetate (see paper)
    35% identity, 97% coverage of query (247 bits)

A4YGX6 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Metallosphaera sedula (see 2 papers)
    35% identity, 97% coverage of query (246 bits)

6abxA Crystal structure of citrate synthase (msed_1522) from metallosphaera sedula in complex with citrate
    35% identity, 97% coverage of query (246 bits)

2c6xA / P39119 Structure of bacillus subtilis citrate synthase
    37% identity, 94% coverage of query (243 bits)

CISY_BACSU / P39119 Citrate synthase 1; Citrate synthase I; EC 2.3.3.16 from Bacillus subtilis (strain 168) (see paper)
    38% identity, 94% coverage of query (243 bits)

citA / CAA74489.1 Citrate synthase I from Bacillus subtilis (see 3 papers)
    38% identity, 94% coverage of query (242 bits)

8bp7E / Q31QM5 Citrate-bound hexamer of synechococcus elongatus citrate synthase (see paper)
    36% identity, 97% coverage of query (235 bits)

Q96ZM7 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Sulfurisphaera tokodaii (see paper)
    34% identity, 97% coverage of query (230 bits)

CISY2_ARATH / Q9LXS6 Citrate synthase 2, peroxisomal; EC 2.3.3.16 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    36% identity, 92% coverage of query (223 bits)

CISY3_ARATH / Q9SJH7 Citrate synthase 3, peroxisomal; EC 2.3.3.16 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    33% identity, 95% coverage of query (223 bits)

6abwA / A4YDF6 Crystal structure of citrate synthase (msed_0281) from metallosphaera sedula in complex with acetyl-coa (see paper)
    34% identity, 95% coverage of query (220 bits)

P9WPD5 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Mycobacterium tuberculosis (see paper)
    35% identity, 97% coverage of query (218 bits)

G8XQY5 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Streptomyces diastaticus (see paper)
    35% identity, 98% coverage of query (218 bits)

P9WPD5 Citrate synthase 1; EC 2.3.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    35% identity, 97% coverage of query (218 bits)

CISY_SACS2 / P80148 Citrate synthase; EC 2.3.3.16 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
P80148 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Saccharolobus solfataricus (see 4 papers)
    31% identity, 97% coverage of query (211 bits)

2h12B / P20901 Structure of acetobacter aceti citrate synthase complexed with oxaloacetate and carboxymethyldethia coenzyme a (cmx) (see paper)
    35% identity, 92% coverage of query (211 bits)

aarA / P20901 citrate synthase monomer (EC 2.3.3.3) from Acetobacter aceti (see 2 papers)
    34% identity, 92% coverage of query (207 bits)

GluT / b0720 citrate synthase (EC 2.3.3.16; EC 2.3.3.1) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
gltA / P0ABH7 citrate synthase (EC 2.3.3.3) from Escherichia coli (strain K12) (see 45 papers)
P0ABH7 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Escherichia coli (see 2 papers)
    34% identity, 96% coverage of query (201 bits)

P0ABH7 Citrate synthase; EC 2.3.3.16 from Escherichia coli (strain K12)
    34% identity, 96% coverage of query (201 bits)

CISYP_DICDI / Q8MQU6 Citrate synthase, peroxisomal; EC 2.3.3.16 from Dictyostelium discoideum (Social amoeba) (see paper)
    31% identity, 97% coverage of query (199 bits)

4jaeA / P0ABH7 Structural determination of the a50t:s279g:s280k:v281k:k282e:h283n variant of citrate synthase from e. Coli complexed with s- carboxymethyl-coa (see paper)
    34% identity, 96% coverage of query (199 bits)

1owbA Three dimensional structure analysis of the variant r109l nadh complex of type ii citrate synthase from e. Coli
    34% identity, 96% coverage of query (199 bits)

4jagA Structural determination of the a50t:s279g:s280k:v281k:k282e:h283n variant of citrate synthase from e. Coli complexed with oxaloacetate
    34% identity, 96% coverage of query (199 bits)

1nxgA The f383a variant of type ii citrate synthase complexed with nadh
    34% identity, 96% coverage of query (197 bits)

CISYC_DICDI / Q86AV6 Citrate synthase; EC 2.3.3.16 from Dictyostelium discoideum (Social amoeba) (see paper)
    31% identity, 92% coverage of query (194 bits)

2ifcC / P21553 The structure of the binary complex of oxalateacetate with citrate synthase from the thermophilic archaeon thermolasma acidophilum
    33% identity, 95% coverage of query (192 bits)

4yboB Structure of citrate synthase from the thermoacidophilic euryarchaeon thermolasma acidophilum
    33% identity, 95% coverage of query (192 bits)

2r9eA The structure of the binary complex of citryl dethia coa and citrate synthase from the thermophilic archaeonthermoplasma acidophilum
    33% identity, 95% coverage of query (192 bits)

2r26A The structure of the ternary complex of carboxymethyl coenzyme a and oxalateacetate with citrate synthase from the thermophilic archaeonthermoplasma acidophilum
    33% identity, 95% coverage of query (192 bits)

ccsA / AAB97876.1 citrate synthase from Rhizobium tropici (see paper)
    31% identity, 97% coverage of query (189 bits)

A0A0H3C985 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Caulobacter vibrioides (see paper)
    31% identity, 97% coverage of query (189 bits)

CISY_PSEAE / P14165 Citrate synthase; EC 2.3.3.16 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    32% identity, 97% coverage of query (188 bits)

3msuA Crystal structure of citrate synthase from francisella tularensis
    31% identity, 96% coverage of query (188 bits)

pcsA / CAA84274.1 citrate synthase from Rhizobium tropici (see paper)
    31% identity, 97% coverage of query (187 bits)

CISY1_ARATH / Q9LXS7 Citrate synthase 1, peroxisomal; EC 2.3.3.16 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    32% identity, 95% coverage of query (186 bits)

3msuB / Q5NIJ6 Crystal structure of citrate synthase from francisella tularensis
    30% identity, 96% coverage of query (183 bits)

4tvmA / P9WPD5 Structure of citrate synthase from mycobacterium tuberculosis (see paper)
    32% identity, 97% coverage of query (180 bits)

8gi7A C143a variant of citrate synthase (cita) in mycobacterium tuberculosis
    30% identity, 95% coverage of query (156 bits)

8gmiA Citrate synthase (cita) in mycobacterium tuberculosis modified by ebselen at c143 residue
    30% identity, 95% coverage of query (156 bits)

8glbD / P9WPD3 Selenomethionine derivatized citrate synthase (cita) in mycobacterium tuberculosis with pyruvate (see paper)
    30% identity, 95% coverage of query (156 bits)

Build an alignment

Build an alignment for Pf6N2E2_6062 and 69 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

8gr9A / P08679 Crystal structure of peroxisomal citrate synthase (cit2) from saccharomyces cerevisiae in complex with oxaloacetate and coenzyme-a (see paper)
    24% identity, 96% coverage of query (105 bits)

CISY2_YEAST / P08679 Citrate synthase, peroxisomal; EC 2.3.3.16 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
    24% identity, 96% coverage of query (103 bits)

CISY1_YEAST / P00890 Citrate synthase, mitochondrial; EC 2.3.3.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
P00890 citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Saccharomyces cerevisiae (see paper)
    24% identity, 91% coverage of query (94.7 bits)

6cscA / P23007 Chicken citrate synthase complex with trifluoroacetonyl-coa and citrate
    22% identity, 92% coverage of query (86.7 bits)

1cssA Alpha-fluoro acid and alpha-fluoro amide analogs of acetyl-coa as inhibitors of of citrate synthase: effect of pka matching on binding affinity and hydrogen bond length
    22% identity, 92% coverage of query (86.7 bits)

1csrA Alpha-fluoro acid and alpha-fluoro amide analogs of acetyl-coa as inhibitors of of citrate synthase: effect of pka matching on binding affinity and hydrogen bond length
    22% identity, 92% coverage of query (86.7 bits)

1csiA A very short hydrogen bond provides only moderate stabilization of an enzyme: inhibitor complex of citrate synthase
    22% identity, 92% coverage of query (86.7 bits)

1cshA A very short hydrogen bond provides only moderate stabilization of an enzyme: inhibitor complex of citrate synthase
    22% identity, 92% coverage of query (86.7 bits)

1amzA Chicken citrate synthase complex with nitromethylde-coa and malate
    22% identity, 92% coverage of query (86.7 bits)

1al6A Chicken citrate synthase complex with n-hydroxyamido-coa and oxaloacetate
    22% identity, 92% coverage of query (86.7 bits)

3enjA Structure of pig heart citrate synthase at 1.78 a resolution
    22% identity, 91% coverage of query (86.3 bits)

2ctsA Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 angstroms resolution
    22% identity, 91% coverage of query (86.3 bits)

CISY_PIG / P00889 Citrate synthase, mitochondrial; Citrate (Si)-synthase; EC 2.3.3.1 from Sus scrofa (Pig) (see paper)
P00889 citrate (Si)-synthase (EC 2.3.3.1); citrate synthase (unknown stereospecificity) (EC 2.3.3.16) from Sus scrofa (see 3 papers)
    22% identity, 91% coverage of query (85.9 bits)

Q29RK1 Citrate synthase, mitochondrial; Citrate (Si)-synthase; EC 2.3.3.1 from Bos taurus (Bovine)
    23% identity, 91% coverage of query (85.5 bits)

6ctsA Proposed mechanism for the condensation reaction of citrate synthase. 1.9-angstroms structure of the ternary complex with oxaloacetate and carboxymethyl coenzyme a
    22% identity, 92% coverage of query (82.0 bits)

3cscA Structure of ternary complexes of citrate synthase with d-and l- malate: mechanistic implications
    22% identity, 92% coverage of query (82.0 bits)

2cscA Structure of ternary complexes of citrate synthase with d-and l- malate: mechanistic implications
    22% identity, 92% coverage of query (82.0 bits)

1cscA Structure of ternary complexes of citrate synthase with d-and l- malate: mechanistic implications
    22% identity, 92% coverage of query (82.0 bits)

CS / O75390 citrate synthase monomer (EC 2.3.3.3) from Homo sapiens (see 4 papers)
CISY_HUMAN / O75390 Citrate synthase, mitochondrial; Citrate (Si)-synthase; EC 2.3.3.1 from Homo sapiens (Human) (see 3 papers)
O75390 citrate (Si)-synthase (EC 2.3.3.1) from Homo sapiens (see 2 papers)
    22% identity, 91% coverage of query (81.6 bits)

5uquB Crystal structure of mutant 2-methylcitrate synthase (mcsag352a) from aspergillus fumigatus
    23% identity, 81% coverage of query (73.6 bits)

5uqrA / B0YD89 Crystal structure of 2-methylcitrate synthase from aspergillus fumigatus (see paper)
    23% identity, 81% coverage of query (72.0 bits)

5uqrB Crystal structure of 2-methylcitrate synthase from aspergillus fumigatus
    23% identity, 81% coverage of query (72.0 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory