Sites on a Tree

 

Searching for up to 100 curated homologs for PfGW456L13_2295 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2295 (270 a.a.)

Found high-coverage hits (≥70%) to 54 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    30% identity, 100% coverage of query (106 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    31% identity, 99% coverage of query (105 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    33% identity, 92% coverage of query (99.8 bits)

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    33% identity, 90% coverage of query (97.1 bits)

Build an alignment

Build an alignment for PfGW456L13_2295 and 4 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    29% identity, 96% coverage of query (86.3 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    27% identity, 92% coverage of query (85.9 bits)

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    29% identity, 97% coverage of query (84.3 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    32% identity, 91% coverage of query (82.8 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    29% identity, 95% coverage of query (80.5 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    29% identity, 95% coverage of query (80.1 bits)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    29% identity, 95% coverage of query (79.7 bits)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    26% identity, 99% coverage of query (74.3 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    30% identity, 96% coverage of query (74.3 bits)

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    28% identity, 99% coverage of query (70.9 bits)

hppC / AAB81313.1 2-hydroxy-6-ketonona-2,4-dienoate hydrolase from Rhodococcus globerulus (see paper)
    27% identity, 98% coverage of query (67.0 bits)

EstN1 / K0IAM1 pimeloyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Nitrososphaera gargensis (strain Ga9.2) (see paper)
    23% identity, 97% coverage of query (61.6 bits)

O73957 carboxylesterase (EC 3.1.1.1) from Sulfolobus acidocaldarius (see paper)
    26% identity, 90% coverage of query (59.7 bits)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
    30% identity, 75% coverage of query (59.7 bits)

6eb3C Structural and enzymatic characterization of an esterase from a metagenomic library
    30% identity, 75% coverage of query (58.9 bits)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
    29% identity, 75% coverage of query (58.2 bits)

BPOC_MYCTU / P9WNH1 Putative non-heme bromoperoxidase BpoC from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    27% identity, 95% coverage of query (56.6 bits)

8agsAAA / A0A1U9WZ52 8agsAAA
    25% identity, 98% coverage of query (56.2 bits)

D0EPY0 carboxylesterase (EC 3.1.1.1) from Bacillus subtilis (see paper)
    26% identity, 91% coverage of query (56.2 bits)

thcF / AAC45285.1 chloroperoxidase from Rhodococcus erythropolis (see 2 papers)
    28% identity, 99% coverage of query (56.2 bits)

carC / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)-hexa-2,4dienoate hydrolase monomer (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
CARC_PSERE / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase; HOPDA; EC 3.7.1.13 from Pseudomonas resinovorans (see 3 papers)
Q9AQM4 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
carC / BAC41548.1 meta cleavage compound hydrolase from Pseudomonas resinovorans (see 9 papers)
    26% identity, 99% coverage of query (56.2 bits)

Q84II3 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13) from Janthinobacterium sp. J3 (see paper)
    26% identity, 99% coverage of query (56.2 bits)

8agnAAA Alpha/beta epoxide hydrolase
    25% identity, 98% coverage of query (56.2 bits)

8agmAAA Alpha/beta epoxide hydrolase
    25% identity, 98% coverage of query (56.2 bits)

8agpAAA Alpha/beta epoxide hydrolase
    25% identity, 98% coverage of query (56.2 bits)

5ng7B Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments
    25% identity, 98% coverage of query (56.2 bits)

NAP_BACSU / P96688 Uncharacterized carboxylesterase nap; EC 3.1.1.1 from Bacillus subtilis (strain 168)
    25% identity, 91% coverage of query (55.8 bits)

estRB8 / CAE54381.1 carboxylesterase, partial from Oleispira antarctica (see paper)
estRB8 / CAE54384.1 carboxylesterase from Oleispira antarctica (see paper)
    23% identity, 94% coverage of query (55.1 bits)

YBFK_BACSU / O31452 Carboxylesterase YbfK; EC 3.1.1.1 from Bacillus subtilis (strain 168) (see paper)
    24% identity, 98% coverage of query (53.5 bits)

6i8wB / Q9KJG6 Crystal structure of a membrane phospholipase a, a novel bacterial virulence factor (see paper)
    24% identity, 97% coverage of query (53.1 bits)

Q9KJG6 triacylglycerol lipase (EC 3.1.1.3) from Pseudomonas aeruginosa (see paper)
    24% identity, 97% coverage of query (53.1 bits)

MhpC / b0349 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
mhpC / P77044 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli (strain K12) (see 10 papers)
MHPC_ECOLI / P77044 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase; 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; EC 3.7.1.14 from Escherichia coli (strain K12) (see 4 papers)
P77044 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase (EC 3.7.1.14) from Escherichia coli (see 4 papers)
    29% identity, 95% coverage of query (52.8 bits)

5h3hB / K0ACL0 Esterase (eaest) from exiguobacterium antarcticum (see paper)
    25% identity, 97% coverage of query (51.6 bits)

PHYLLO / Q15KI9 PHYLLO [multifunctional] (EC 4.2.99.20; EC 3.1.2.28; EC 4.2.1.113; EC 2.2.1.9) from Arabidopsis thaliana (see 6 papers)
PHYLO_ARATH / Q15KI9 Protein PHYLLO, chloroplastic; EC 2.2.1.9; EC 4.2.1.113; EC 4.2.99.20 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    24% identity, 97% coverage of query (49.7 bits)

nahN / BAE92169.1 2-hydroxymuconic semialdehyde hydrolase NahN from Pseudomonas putida (see 2 papers)
    29% identity, 97% coverage of query (49.3 bits)

5jzbA Crystal structure of hsad bound to 3,5-dichlorobenzene sulphonamide
    29% identity, 78% coverage of query (48.1 bits)

7zm4A Crystal structure of hsad from mycobacterium tuberculosis in complex with cyclipostin-like inhibitor cyc31
    29% identity, 78% coverage of query (47.8 bits)

7zm3A Crystal structure of hsad from mycobacterium tuberculosis in complex with cyclipostin-like inhibitor cyc17
    29% identity, 78% coverage of query (47.8 bits)

7zm2A Crystal structure of hsad from mycobacterium tuberculosis in complex with cyclophostin-like inhibitor cyc8b
    29% identity, 78% coverage of query (47.8 bits)

7zm1A Crystal structure of hsad from mycobacterium tuberculosis in complex with cyclophostin-like inhibitor cyc7b
    29% identity, 78% coverage of query (47.8 bits)

5jzsB Hsad bound to 3,5-dichloro-4-hydroxybenzoic acid
    29% identity, 78% coverage of query (47.8 bits)

5jz9A / P9WNH5 Crystal structure of hsad bound to 3,5-dichloro-4- hydroxybenzenesulphonic acid (see paper)
    29% identity, 78% coverage of query (47.8 bits)

hsaD / P9WNH5 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase monomer (EC 3.7.1.17) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
HSAD_MYCTU / P9WNH5 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase; 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase; EC 3.7.1.17; EC 3.7.1.8 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
P9WNH5 2,6-dioxo-6-phenylhexa-3-enoate hydrolase (EC 3.7.1.8) from Mycobacterium tuberculosis (see paper)
    29% identity, 78% coverage of query (47.8 bits)

2wufB Crystal structure of s114a mutant of hsad from mycobacterium tuberculosis in complex with 4,9dsha
    28% identity, 78% coverage of query (47.0 bits)

2wueB Crystal structure of s114a mutant of hsad from mycobacterium tuberculosis in complex with hopoda
    28% identity, 78% coverage of query (47.0 bits)

2wugA Crystal structure of s114a mutant of hsad from mycobacterium tuberculosis in complex with hopda
    28% identity, 78% coverage of query (47.0 bits)

RMS3_PEA / A0A109QYD3 Strigolactone esterase RMS3; Protein DWARF 14 homolog; PsD14; Protein RAMOSUS 3; EC 3.1.-.- from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see paper)
    24% identity, 91% coverage of query (46.6 bits)

5cbkA / A0A0M5I297 Crystal structure of the strigolactone receptor shhtl5 from striga hermonthica (see paper)
    23% identity, 91% coverage of query (44.7 bits)

4jymA / Q9SZU7 Crystal structure of kai2 in complex with 3-methyl-2h-furo[2,3- c]pyran-2-one (see paper)
    23% identity, 91% coverage of query (44.3 bits)

KAI2_ARATH / Q9SZU7 Probable esterase KAI2; Protein DWARF-14-like; Protein D14-like; Protein HYPOSENSITIVE TO LIGHT; Protein KARRIKIN INSENSITIVE 2 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
    23% identity, 91% coverage of query (44.3 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory