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Searching for up to 100 curated homologs for RR42_RS26020 FitnessBrowser__Cup4G11:RR42_RS26020 (282 a.a.)

Found high-coverage hits (≥70%) to 34 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

NIT2_RAT / Q497B0 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Rattus norvegicus (Rat) (see 2 papers)
    31% identity, 85% coverage of query (101 bits)

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Build an alignment for RR42_RS26020 and 1 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

NIT2_HUMAN / Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 4 papers)
Q9NQR4 omega-amidase (EC 3.5.1.3) from Homo sapiens (see paper)
    28% identity, 85% coverage of query (95.9 bits)

Nit2 / Q9JHW2 ω-amidase NIT2 monomer (EC 3.5.1.3) from Mus musculus (see 2 papers)
NIT2_MOUSE / Q9JHW2 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Mus musculus (Mouse) (see 3 papers)
    29% identity, 85% coverage of query (95.1 bits)

AMIF_HELPY / O25836 Formamidase; Formamide amidohydrolase; EC 3.5.1.49 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see 2 papers)
    28% identity, 88% coverage of query (86.3 bits)

mtnU / O31664 2-oxoglutaramate:glutamine aminotransferase (EC 3.5.1.3) from Bacillus subtilis (strain 168) (see 4 papers)
MTNU_BACSU / O31664 2-oxoglutaramate amidase; EC 3.5.1.111 from Bacillus subtilis (strain 168) (see 2 papers)
    27% identity, 84% coverage of query (84.3 bits)

AMIE_HELPY / O25067 Aliphatic amidase; Acylamide amidohydrolase; EC 3.5.1.4 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see 3 papers)
    25% identity, 79% coverage of query (83.6 bits)

P59701 formamidase (EC 3.5.1.49) from Bacillus cereus (see paper)
    28% identity, 84% coverage of query (83.6 bits)

2e2lA / O25836 Helicobacter pylori formamidase amif contains a fine-tuned cysteine- glutamate-lysine catalytic triad (see paper)
    28% identity, 87% coverage of query (83.2 bits)

YHCX_BACSU / P54608 Probable hydrolase YhcX; EC 3.5.-.- from Bacillus subtilis (strain 168) (see paper)
    28% identity, 85% coverage of query (82.8 bits)

6ypaB / O58376 The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
    31% identity, 73% coverage of query (77.4 bits)

7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide
    31% identity, 73% coverage of query (77.4 bits)

YCU9_SCHPO / O59829 Probable nitrilase C965.09; EC 3.5.-.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    25% identity, 84% coverage of query (77.0 bits)

AMIE_PSEAE / P11436 Aliphatic amidase; Acylamide amidohydrolase; EC 3.5.1.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
P11436 amidase (EC 3.5.1.4) from Pseudomonas aeruginosa (see paper)
    26% identity, 83% coverage of query (75.5 bits)

AMIE_BACSP / Q9L543 Aliphatic amidase; Acylamide amidohydrolase; EC 3.5.1.4 from Bacillus sp. (see paper)
    26% identity, 79% coverage of query (73.9 bits)

3klcA / Q9UYV8 Crystal structure of hyperthermophilic nitrilase (see paper)
    29% identity, 73% coverage of query (73.9 bits)

3klcB Crystal structure of hyperthermophilic nitrilase
    29% identity, 73% coverage of query (73.9 bits)

NITR_PYRAB / Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see 2 papers)
    29% identity, 73% coverage of query (73.2 bits)

YAFV_STAA4 / P0DP65 Omega-amidase YafV; saYafV; EC 3.5.1.3 from Staphylococcus aureus (strain 04-02981) (see paper)
    26% identity, 73% coverage of query (72.8 bits)

AMIE_RHOER / Q01360 Aliphatic amidase; Acylamide amidohydrolase; Wide spectrum amidase; EC 3.5.1.4 from Rhodococcus erythropolis (Arthrobacter picolinophilus)
    27% identity, 79% coverage of query (72.4 bits)

AMIE_GEOSE / Q9RQ17 Aliphatic amidase; Acylamide amidohydrolase; Wide spectrum amidase; EC 3.5.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
    26% identity, 79% coverage of query (72.0 bits)

4gylA / Q9L543 The e142l mutant of the amidase from geobacillus pallidus showing the result of michael addition of acrylamide at the active site cysteine (see paper)
    26% identity, 79% coverage of query (71.2 bits)

NIT1_YEREN / P0DP68 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; yeNit1; EC 3.5.1.128 from Yersinia enterocolitica (see paper)
P0DP68 deaminated glutathione amidase (EC 3.5.1.128) from Yersinia enterocolitica (see paper)
    25% identity, 85% coverage of query (70.5 bits)

B8A2V8 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    26% identity, 85% coverage of query (70.1 bits)

B8A0E5 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    27% identity, 85% coverage of query (69.7 bits)

ramA / Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
RSAM_PSESP / Q75SP7 (R)-stereoselective amidase; EC 3.5.1.100 from Pseudomonas sp. (see paper)
Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
    29% identity, 72% coverage of query (68.6 bits)

pydC / Q846U4 3-amino-isobutyrate synthase (EC 3.5.1.6) from Brevibacillus agri (see paper)
    29% identity, 73% coverage of query (66.2 bits)

4iztA / D0VWZ1 The e41q mutant of the amidase from nesterenkonia sp. An1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine
    29% identity, 71% coverage of query (62.0 bits)

4izuA The e41q mutant of the amidase from nesterenkonia sp. An1 showing the result of michael addition of acrylamide at the active site cysteine
    29% identity, 71% coverage of query (61.2 bits)

NFT1_DROME / O76464 Nitrilase and fragile histidine triad fusion protein NitFhit; NFT-1 protein; EC 3.6.1.29; EC 3.5.-.- from Drosophila melanogaster (Fruit fly) (see paper)
    25% identity, 72% coverage of query (60.8 bits)

A0A023XI92 N-carbamoyl-D-amino-acid hydrolase (EC 3.5.1.77) from Bradyrhizobium japonicum (see paper)
    27% identity, 83% coverage of query (59.3 bits)

5nybA A c145a mutant of nesterenkonia an1 amidase bound to adipamide
    29% identity, 71% coverage of query (58.9 bits)

5ny7A A c145a mutant of nesterenkonia an1 amidase bound to nicotinamide
    29% identity, 71% coverage of query (58.9 bits)

5nycA A c145a mutant of nesterenkonia an1 amidase bound to propionitrile
    29% identity, 71% coverage of query (58.5 bits)

4izsA The c145a mutant of the amidase from nesterenkonia sp. An1 in complex with butyramide
    29% identity, 71% coverage of query (58.5 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory