Sites on a Tree

 

Searching for up to 100 curated homologs for RR42_RS36980 FitnessBrowser__Cup4G11:RR42_RS36980 (224 a.a.)

Found high-coverage hits (≥70%) to 30 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO353_16285 ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 2 from Pseudomonas fluorescens FW300-N2E3
    38% identity, 73% coverage of query (103 bits)

Pf1N1B4_772 ABC transporter for L-asparagine and L-glutamate, permease component 1 from Pseudomonas fluorescens FW300-N1B4
    38% identity, 73% coverage of query (100 bits)

PfGW456L13_4771 ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 1 from Pseudomonas fluorescens GW456-L13
    38% identity, 73% coverage of query (100 bits)

TC 3.A.1.3.19 / Q88NY3 PP1070, component of Acidic amino acid uptake porter, AatJMQP from Pseudomonas putida (strain KT2440) (see paper)
    38% identity, 77% coverage of query (100 bits)

TC 3.A.1.3.22 / Q9I403 Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    33% identity, 89% coverage of query (94.0 bits)

TC 3.A.1.3.25 / Q9CES5 Glutamine ABC transporter permease and substrate binding protein protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity from Lactococcus lactis subsp. lactis (strain IL1403)
    30% identity, 92% coverage of query (90.9 bits)

TC 3.A.1.3.17 / Q8YSA2 Basic amino acid uptake transporter, BgtAB (see paper)
    31% identity, 97% coverage of query (89.4 bits)

GltJ / b0654 glutamate/aspartate ABC transporter membrane subunit GltJ (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
GltJ / P0AER3 glutamate/aspartate ABC transporter membrane subunit GltJ (EC 7.4.2.1) from Escherichia coli (strain K12) (see 2 papers)
GLTJ_ECOLI / P0AER3 Glutamate/aspartate import permease protein GltJ from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.3.4 / P0AER3 Glutamate/aspartate transport system permease protein GltJ aka B0654, component of Glutamate/aspartate porter from Escherichia coli (see 4 papers)
    30% identity, 89% coverage of query (87.8 bits)

GLNM_BACSU / O34671 Probable glutamine ABC transporter permease protein GlnM from Bacillus subtilis (strain 168) (see paper)
    30% identity, 86% coverage of query (85.5 bits)

TcyL / b1918 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
tcyL / P0AFT2 cystine ABC transporter membrane subunit (EC 7.4.2.12) from Escherichia coli (strain K12) (see 3 papers)
TCYL_ECOLI / P0AFT2 L-cystine transport system permease protein TcyL from Escherichia coli (strain K12) (see 5 papers)
TC 3.A.1.3.10 / P0AFT2 Inner membrane amino-acid ABC transporter permease protein YECS CysY aka B1918, component of Cystine/cysteine/diaminopimelate transporter, CysXYZ; these proteins are also designated FliY/YecS/YecC from Escherichia coli (see 4 papers)
    31% identity, 88% coverage of query (85.5 bits)

Build an alignment

Build an alignment for RR42_RS36980 and 10 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

TC 3.A.1.3.12 / P73544 BgtB aka GLNH aka SLL1270, component of Arginine/lysine/histidine/glutamine porter (see paper)
    29% identity, 90% coverage of query (77.0 bits)

4ymtC / Q8RCC3 Crystal structure of an amino acid abc transporter complex with arginines (see paper)
    26% identity, 91% coverage of query (74.7 bits)

4ymwC Crystal structure of an amino acid abc transporter with histidines
    26% identity, 91% coverage of query (74.7 bits)

TCYB_BACSU / P42200 L-cystine transport system permease protein TcyB from Bacillus subtilis (strain 168) (see paper)
TC 3.A.1.3.14 / P42200 TcyB (YckJ), component of Uptake system for L-cystine from Bacillus subtilis (see 4 papers)
    32% identity, 80% coverage of query (73.6 bits)

TC 3.A.1.3.16 / A1VZQ3 PEP1B, component of Uptake system for glutamate and aspartate from Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
    28% identity, 87% coverage of query (72.4 bits)

TC 3.A.1.3.5 / P35114 OCM1 aka OccM, component of Octopine porter from Agrobacterium tumefaciens (see 2 papers)
    31% identity, 89% coverage of query (72.0 bits)

GLUC_CORGL / P48244 Glutamate transport system permease protein GluC; Glutamate uptake system protein GluC from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
TC 3.A.1.3.9 / P48244 GluC aka CGL1952, component of Glutamate porter from Corynebacterium glutamicum (Brevibacterium flavum) (see 2 papers)
    30% identity, 75% coverage of query (69.7 bits)

TCYL_BACSU / O34315 L-cystine transport system permease protein TcyL from Bacillus subtilis (strain 168) (see 2 papers)
TC 3.A.1.3.13 / O34315 TcyL (YtmL), component of Uptake system for L-cystine (Km=2.5 μM), L-cystathionine, L-djenkolate ( 2-amino-3-[(2-amino-3-hydroxy-3-oxopropyl)sulfanylmethylsulfanyl] propanoic acid), and S-methyl-L-cysteine from Bacillus subtilis (see 4 papers)
    28% identity, 92% coverage of query (67.0 bits)

ARGU_CORGL / Q8NQU3 Arginine transport system permease protein ArgU from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
    31% identity, 76% coverage of query (67.0 bits)

TC 3.A.1.3.6 / P35113 NocM aka ATU6025 aka AGR_PTI_65, component of Nopaline porter from Agrobacterium tumefaciens (see 3 papers)
    32% identity, 73% coverage of query (66.6 bits)

YXEN_BACSU / P54953 Probable amino-acid permease protein YxeN from Bacillus subtilis (strain 168) (see paper)
    29% identity, 80% coverage of query (66.6 bits)

GLNP_BACSU / O34606 Probable glutamine ABC transporter permease protein GlnP from Bacillus subtilis (strain 168) (see paper)
    27% identity, 82% coverage of query (65.5 bits)

ArtQ / b0862 L-arginine ABC transporter membrane subunit ArtQ (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
ArtQ / P0AE34 L-arginine ABC transporter membrane subunit ArtQ (EC 7.4.2.1) from Escherichia coli (strain K12) (see 2 papers)
ARTQ_ECOLI / P0AE34 Arginine ABC transporter permease protein ArtQ from Escherichia coli (strain K12) (see paper)
TC 3.A.1.3.3 / P0AE34 Arginine transport system permease protein ArtQ aka B0862, component of Arginine porter from Escherichia coli (see 6 papers)
    27% identity, 92% coverage of query (65.1 bits)

AO356_00475 ABC transporter for D-Glucosamine, permease component 2 from Pseudomonas fluorescens FW300-N2C3
    29% identity, 84% coverage of query (64.3 bits)

Pf6N2E2_2052 ABC transporter for D-Glucosamine Hydrochloride, permease component 2 from Pseudomonas fluorescens FW300-N2E2
    29% identity, 84% coverage of query (64.3 bits)

TCYM_BACSU / O34931 L-cystine transport system permease protein TcyM from Bacillus subtilis (strain 168) (see 2 papers)
TC 3.A.1.3.13 / O34931 TcyM (YtmM), component of Uptake system for L-cystine (Km=2.5 μM), L-cystathionine, L-djenkolate ( 2-amino-3-[(2-amino-3-hydroxy-3-oxopropyl)sulfanylmethylsulfanyl] propanoic acid), and S-methyl-L-cysteine from Bacillus subtilis (see 4 papers)
    26% identity, 96% coverage of query (63.9 bits)

TC 3.A.1.3.11 / O50183 AotP aka AotM aka PA0890, component of Arginine/ornithine (but not lysine) porter from Pseudomonas aeruginosa (see 2 papers)
    30% identity, 74% coverage of query (63.2 bits)

AO353_21715 ABC transporter for D-glucosamine, permease component 1 from Pseudomonas fluorescens FW300-N2E3
    29% identity, 84% coverage of query (63.2 bits)

Ac3H11_2554 ABC transporter for L-Histidine, permease component 1 from Acidovorax sp. GW101-3H11
    25% identity, 85% coverage of query (62.0 bits)

PS417_21735 ABC transporter for L-Arginine, permease component 2 from Pseudomonas simiae WCS417
    28% identity, 74% coverage of query (60.1 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory